İstanbul Journal of Pharmacy

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DOI :10.5152/IstanbulJPharm.2018.397094   IUP :10.5152/IstanbulJPharm.2018.397094    Full Text (PDF)

A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation

Cem Kaplan, Duri Şehvar Ünal

A high performance liquid chromatography method has been developed for determination of letrozole in tablets. The method has developed in reverse phase column with acetonitrile-50.0 mM phosphoric acid solution, pH:7 (50:50, v/v) and 0.7 mL/min flow rate by using a fluorimetric detector. Letrozole has natural fluorescence properties, and there is no need for derivatization for this molecule. The detector was set at 256, 585 nm for excitation and emission wavelength respectively. The method was validated in accuracy, precision, specificity, linearity and system suitability parameters are studied. The linear range was found 50-700 ng/mL. The limit of detection and quantification for letrozole was found to be 14-50 ng/mL respectively. The recovery of drug is found to be 98.8%. The developed method has been validated and successfully applied to tablet analysis of drug substance. This method is simple, reproducible and can be used safely for routine analysis of letrozole in tablets.

Keywords: Letrozole, HPLC-FLD, pharmaceutical preparation

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Citations

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APA

Kaplan, C., & Ünal, D. (2018). A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation. İstanbul Journal of Pharmacy, 48(2), 38-42. https://doi.org/10.5152/IstanbulJPharm.2018.397094


AMA

Kaplan C, Ünal D. A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation. İstanbul Journal of Pharmacy. 2018;48(2):38-42. https://doi.org/10.5152/IstanbulJPharm.2018.397094


ABNT

Kaplan, C.; Ünal, D. A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation. İstanbul Journal of Pharmacy, [Publisher Location], v. 48, n. 2, p. 38-42, 2018.


Chicago: Author-Date Style

Kaplan, Cem, and Duri Şehvar Ünal. 2018. “A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation.” İstanbul Journal of Pharmacy 48, no. 2: 38-42. https://doi.org/10.5152/IstanbulJPharm.2018.397094


Chicago: Humanities Style

Kaplan, Cem, and Duri Şehvar Ünal. “A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation.” İstanbul Journal of Pharmacy 48, no. 2 (Apr. 2025): 38-42. https://doi.org/10.5152/IstanbulJPharm.2018.397094


Harvard: Australian Style

Kaplan, C & Ünal, D 2018, 'A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation', İstanbul Journal of Pharmacy, vol. 48, no. 2, pp. 38-42, viewed 29 Apr. 2025, https://doi.org/10.5152/IstanbulJPharm.2018.397094


Harvard: Author-Date Style

Kaplan, C. and Ünal, D. (2018) ‘A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation’, İstanbul Journal of Pharmacy, 48(2), pp. 38-42. https://doi.org/10.5152/IstanbulJPharm.2018.397094 (29 Apr. 2025).


MLA

Kaplan, Cem, and Duri Şehvar Ünal. “A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation.” İstanbul Journal of Pharmacy, vol. 48, no. 2, 2018, pp. 38-42. [Database Container], https://doi.org/10.5152/IstanbulJPharm.2018.397094


Vancouver

Kaplan C, Ünal D. A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation. İstanbul Journal of Pharmacy [Internet]. 29 Apr. 2025 [cited 29 Apr. 2025];48(2):38-42. Available from: https://doi.org/10.5152/IstanbulJPharm.2018.397094 doi: 10.5152/IstanbulJPharm.2018.397094


ISNAD

Kaplan, Cem - Ünal, Duri Şehvar. “A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation”. İstanbul Journal of Pharmacy 48/2 (Apr. 2025): 38-42. https://doi.org/10.5152/IstanbulJPharm.2018.397094



TIMELINE


Accepted19.07.2018

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