İstanbul Journal of Pharmacy

SUBMITINFORMATION
EnglishTürkçe

Research Article


DOI :10.26650/IstanbulJPharm.2024.1431886   IUP :10.26650/IstanbulJPharm.2024.1431886    Full Text (PDF)

Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells

Zeynep Özgen Bölükbaşı, Cemre Özkanca, Ayşe Seher Birteksöz Tan

Background and Aims: Streptococcus anginosus and Candida albicans exert negative effects on oral health and can cause biofilm formation on tooth surfaces. Partial or total denture surfaces used by people who have lost their teeth due to various reasons are sensitive to microorganism invasion and biofilm formation, similar to tooth surfaces. Therefore, this study was conducted to investigate the antibiofilm activities of various denture cleaning tablets (DCTs) used to disinfect dentures against S. anginosus clinical isolates and C. albicans standard strain and their cytotoxic effects against oral epithelial cells.

Methods: The biofilm-forming abilities of strains were determined using the crystal violet assay. The modified time-killing curve (TKC) method was used to evaluate the dynamic bactericidal or fungicidal activities of DCTs against biofilms of S. anginosus isolates and C. albicans ATCC 10231. In vitro cytotoxicity experiments of DCTs on human gingival fibroblast cell lines (HGF-1) were also conducted using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide staining method.

Results: Overall, 18.18% and 36.36% of S. anginosus isolates were strong and moderate biofilm formers, respectively. C. albicans ATCC 10231 also formed a strong biofilm. The TKC analysis revealed that all the examined DCTs inhibited almost all living cells in mature biofilms at every time point. The cytotoxic activities of DCTs against HGF-1 cell lines were in the range of 93%–95% at their direct usage concentrations.

Conclusion: DCTs exhibit rapid and strong activity against biofilms, which is extremely important for biofilm-related infections. Nevertheless, it is necessary to consider the cytotoxic effects of DCTs on HGF-1 cells for consumers’ oral healt.

Keywords: Biofilm, denture cleaning tablet, cytotoxic effects, Streptococcus anginosus

PDF View

References

  • Andrighetti, Fröhner., C.R., Antonio, R.V., Creczynski-Pasa, T.B., Barardi, C. R.M., Simöes, C.M.O. (2003). Cytotoxicity and po-tential antiviral evaluation of violacein produced by Chromobac-terium violaceum. Memorias do Instituto Oswaldo Cruz, 98, 843848. google scholar
  • Apratim, A., Shah, S. S., Sinha, M., Agraval M, Chhaparia, N., Abubakkar, A. (2013). Denture hygiene habits among elderly pa-tients wearing complete dentures. Journal of Contemporary Den-tal Practice, 14, 1161-1164. google scholar
  • Budtz-Jorgensen E., Loe, H. (1972). Chlorhexidine as a denture disin-fectant in the treatment of denture stomatitis. Scandinavian Jour-nal of Dental Research, 80, 457-464. google scholar
  • Dosler, S., Karaaslan, E. (2014). Inhibition and destruction of Pseu-domonas aeruginosa biofilms by antibiotics and antimicrobial pep-tides. Peptides, 62, 32-37. google scholar
  • Emami, E., Kabawat, M., Rompre, P.H., Feine, J.S. (2014). Linking evidence to treatment for denture stomatitis: A meta-analysis of randomized controlled trials. Journal of Dentistry, 42, 99-106. google scholar
  • Gendreau, L., & Zg, L. (2011). Epidemiology and etiology of denture stomatitis. Journal of Prosthodontics, 20, 251-260. google scholar
  • Heller, D., Helmerhorst, E. J., Gower, A. C., Siqueira, W. L., Paster, B. J., & Oppenheim, F. G. (2016). Microbial Diversity in the Early In Vivo-Formed Dental Biofilm. Applied and Environmental Microbiology, 82, 1881-1888. google scholar
  • Jenkinson, H.F. (1994). Cell surface protein receptors in oral strepto-cocci. FEMS Microbiology Letters,121(2),133-140. google scholar
  • Jose, A., Coco, B. J., Milligan, S., Young, B., Lappin, D.F., Bagg, J....Ramage, G. (2010). Reducing the incidence of denture stomatitis: Are denture cleansers sufficient? Journal of Prosthodontics, 19, 252-257. 10.1111/j.1532-849X.2009.00561.x google scholar
  • Li, J., Helmerhorst, E. J., Leone, C. W., Troxler, R. F., Yaskell, T., Haffajee, A. D., Socransky, S. S., & Oppenheim, F. G. (2004). Identification of early microbial colonizers in human dental biofilm. Journal of Applied Microbiology, 97, 1311-1318. https://doi.org/10.1111/j.1365-2672.2004.02420.x google scholar
  • Martori, E., Ayuso-Montero, R., Martinez-Gomis, J., Vinas, Miquel., Peraire, M. (2014). Risk factors for denture-related oral mucosal lesions in a geriatric population. The Journal of Prosthetic Den-tistry, 111, 273-279. doi: 10.1016/j.prosdent.2013.07.015. google scholar
  • Nirwati, H., Sinanjung, K., Fahrunissa, F., Fernando, W., Napitupulu, S., Hati, V.P.... Nuryastuti, T. (2019). Biofilm formation and an-tibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia. BMC, 13, 1-8 .https://doi.org/10.1186/s12919-019-0176-7 google scholar
  • Nobbs, A. H., Lamont, R. J., & Jenkinson, H. F. (2009). Streptococcus adherence and colonization. Microbiology and Molecular Biology Reviews, 73, 407-450. https://doi.org/10.1128/MMBR.00014-09. google scholar
  • Orgini, D. B., Silva-Lovato, C. H., de Souza, R. F., & et al. (2012). Abrasiveness of conventional and specific denture-cleansing den-tifrices. Brazilian Dental Journal, 23, 154-159. google scholar
  • Ramage, G., Saville, S.P., Thomas, D. P., et al. (2005). Candida biofilms: An update. Eukaryotic Cell, 4, 633-688. google scholar
  • Ramage, G., O’Donnell, L., Sherry, L., Culshaw, S., Bagg, J., Czesnikiewicz-Guzik, M., Brown, C.... Robertson, D. (2018). Im-pact of frequency of denture cleaning on microbial and clinical pa-rameters - a bench to chairside approach. Journal of Oral Microbi-ology, 11, 1-15. https://doi.org/10.1080/20002297.2018.1538437 google scholar
  • Ritz, H. L. (1967). Microbial population shifts in developing human dental plaque. Archives of Oral Biology, 12, 1568-1570. google scholar
  • Ruhl, S., Eidt, A., Melzl, H., Reischl, U., & Cisar, J. O. (2014). Probing of microbial biofilm communities for coadhesion part-ners. Applied and Environmental Microbiology, 80, 6583-6590. https://doi.org/10.1128/AEM.01826-14. google scholar
  • Shankar, T., Gowd, S., Suresan, V., Mantri, S., Saxena, S., Mishra, P,. Panday, P. (2017) Denture Hygiene Knowledge and Practices among Complete Denture Wearers attending a Postgraduate Den-tal Institute. Journal of Contemporary Dental Practice, 18, 714721. google scholar
  • Srinivasan, M., & Gulabani, M. (2010). A microbiological evaluation of the use of denture cleansers in combination with an oral rinse in complete denture patients. Indian Journal of Dental Research, 21, 353-356. google scholar
  • Takamiya, A.S.; Monteiro, D.R.; Barâo, V.A.R.; Barbosa, D.B.; Pero, A.C, Compagnoni, M.A. (2011). Complete denture hy-giene and nocturnal wearing habits among patients attending the Prosthodontic Department in a Dental University in Brazil. Gerodontology, 28, 91-96. google scholar
  • Verran, J., Jackson, S., Coulthwaite, L., & et al. (2014). The effect of dentifrice abrasion on denture topography and the subsequent retention of microorganisms on abraded surfaces. Journal of Pros-thetic Dentistry, 112, 1513-1522. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Özgen Bölükbaşı, Z., Özkanca, C., & Birteksöz Tan, A.S. (2024). Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells. İstanbul Journal of Pharmacy, 54(1), 89-95. https://doi.org/10.26650/IstanbulJPharm.2024.1431886


AMA

Özgen Bölükbaşı Z, Özkanca C, Birteksöz Tan A S. Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells. İstanbul Journal of Pharmacy. 2024;54(1):89-95. https://doi.org/10.26650/IstanbulJPharm.2024.1431886


ABNT

Özgen Bölükbaşı, Z.; Özkanca, C.; Birteksöz Tan, A.S. Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells. İstanbul Journal of Pharmacy, [Publisher Location], v. 54, n. 1, p. 89-95, 2024.


Chicago: Author-Date Style

Özgen Bölükbaşı, Zeynep, and Cemre Özkanca and Ayşe Seher Birteksöz Tan. 2024. “Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells.” İstanbul Journal of Pharmacy 54, no. 1: 89-95. https://doi.org/10.26650/IstanbulJPharm.2024.1431886


Chicago: Humanities Style

Özgen Bölükbaşı, Zeynep, and Cemre Özkanca and Ayşe Seher Birteksöz Tan. “Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells.” İstanbul Journal of Pharmacy 54, no. 1 (Nov. 2024): 89-95. https://doi.org/10.26650/IstanbulJPharm.2024.1431886


Harvard: Australian Style

Özgen Bölükbaşı, Z & Özkanca, C & Birteksöz Tan, AS 2024, 'Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells', İstanbul Journal of Pharmacy, vol. 54, no. 1, pp. 89-95, viewed 15 Nov. 2024, https://doi.org/10.26650/IstanbulJPharm.2024.1431886


Harvard: Author-Date Style

Özgen Bölükbaşı, Z. and Özkanca, C. and Birteksöz Tan, A.S. (2024) ‘Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells’, İstanbul Journal of Pharmacy, 54(1), pp. 89-95. https://doi.org/10.26650/IstanbulJPharm.2024.1431886 (15 Nov. 2024).


MLA

Özgen Bölükbaşı, Zeynep, and Cemre Özkanca and Ayşe Seher Birteksöz Tan. “Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells.” İstanbul Journal of Pharmacy, vol. 54, no. 1, 2024, pp. 89-95. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2024.1431886


Vancouver

Özgen Bölükbaşı Z, Özkanca C, Birteksöz Tan AS. Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells. İstanbul Journal of Pharmacy [Internet]. 15 Nov. 2024 [cited 15 Nov. 2024];54(1):89-95. Available from: https://doi.org/10.26650/IstanbulJPharm.2024.1431886 doi: 10.26650/IstanbulJPharm.2024.1431886


ISNAD

Özgen Bölükbaşı, Zeynep - Özkanca, Cemre - Birteksöz Tan, AyşeSeher. “Antibiofilm activities of denture cleaning tablets against Streptococcus anginosus - Cytotoxic effects on human gingival fibroblast cells”. İstanbul Journal of Pharmacy 54/1 (Nov. 2024): 89-95. https://doi.org/10.26650/IstanbulJPharm.2024.1431886



TIMELINE


Submitted05.02.2024
Accepted17.03.2024
Published Online30.04.2024

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.