Research Article


DOI :10.26650/IstanbulJPharm.2022.998774   IUP :10.26650/IstanbulJPharm.2022.998774    Full Text (PDF)

Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens

Müjgan ÖzfenerciElif Burcu BaliDemet ErdönmezUfuk Koca Çalışkan

Background and Aims: Aromatherapy, as one of the complementary therapies, uses essential oils as the main therapeutic agents to treat several diseases. In the present study, it was aimed to investigate inhibition of quorum sensing (QS) and biofilm formation of aromatherapeutic essential oils (AEOs) and their pharmaceutical combinations (PC-I and PC-II). Methods: The anti-QS potential of AEOs were determined using the biosensor strains Chromobacterium violaceum ATCC 12472 and Pseudomonas aeruginosa PAO1. Anti-QS activity was detected by agar-well diffusion and violacein pigment inhibition assays. Blocking of PAO1 swim and swarm motilities and biofilm formation was also performed. Results: Most of the AEOs demonstrated highly active (>95%) violacein pigment inhibition. Additionally, they inhibited swarming (40.34%-72.80%) and swimming (20.06%-50.08%) motilities of PAO1. Moreover, the majority of AEOs also decreased the biofilm formation, particularly on P. aeruginosa and S. aureus. Conclusion: Consequently, aromatherapeutic formulations might be a complementary or prophylactic cure for infectious disease by their anti-QS and antibiofilm activities rather than just antimicrobial effects.


PDF View

References

  • Ahmad, A., Viljoen, A., & Chenia, H. (2015). The impact of plant volatiles on bacterial quorum sensing. Letters in Applied Microbiol-ogy, 60(1), 8-19. https://doi.org/10.1111/lam.12343 google scholar
  • Ali, B., Al-Wabel, N. A., Shams, S., Ahamad, A., Khan, S. A., & Anwar, F. (2015). Essential oils used in aromatherapy: A systemic review. Asian Pacific Journal of Tropical Biomedicine, 5(8), 601-611. https:// doi.org/10.1016/j.apjtb.2015.05.007 google scholar
  • Alibi, S., Selma, W. B., Ramos-Vivas, J., Smach, M. A., Touati, R., Boukadida, J., Navas, J. & Mansour, H. B. (2020). Anti-oxidant, anti-bacterial, anti-biofilm, and anti-quorum sensing activities of four essential oils against multidrug-resistant bacterial clinical isolates. Current Research in Translational Medicine, 68(2), 59-66.https://doi. org/10.1016/j.retram.2020.01.001 google scholar
  • Bai A, J., & Vittal, R. R. (2014). Quorum sensing inhibitory and anti-biofilm activity of essential oils and their in vivo efficacy in food systems. Food Biotechnology, 28(3), 269-292. https://doi.org/10.1 080/08905436.2014.932287 google scholar
  • Bharti, S. K., Pathak, V., Alam, T., Arya, A., Singh, V. K., Verma, A. K., & Rajkumar, V. (2020). Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicro-bial and antioxidative aspect of chicken nuggets during extend-ed storage. Journal of Food Science, 85(9), 2857-2865. https://doi. org/10.1111/1750-3841.15365 google scholar
  • Blosser, R. S., & Gray, K. M. (2000). Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioas-say for N-acyl homoserine lactone autoinducers. Journal of Micro-biological Methods, 40(1), 47-55. https://doi.org/10.1016/S0167-7012(99)00136-0 google scholar
  • Boire, N. A., Riedel, S., & Parrish, N. M. (2013). Essential oils and future antibiotics: new weapons against emerging ‘superbugs’. Journal of Ancient Diseases & Preventive Remedies, 1(2), 1000105. https://doi.org/10.4172/jadpr.1000105 google scholar
  • Brun, P,, Bernabe, G., Filippini, R., & Piovan, A. (2019). In vitro anti-microbial activities of commercially available tea tree (Melaleuca alternifolia) essential oils. Current Microbiology, 76(1), 108-116. https://doi.org/10.1007/s00284-018-1594-x. google scholar
  • Bali, E. B., Erkan Türkmen, K. , Erdönmez, D., & Sağlam, N. (2019). Comparative study of inhibitory potential of dietary phytochemi-cals against quorum sensing activity of and biofilm formation by Chromobacterium violaceum 12472, and swimming and swarm-ing behaviour of Pseudomonas aeruginosa PAO1. Food Technol-ogy and Biotechnology, 57(2), 212-221. https://doi.org/10.17113/ ftb.57.02.19.5823 google scholar
  • Caceres, M., Hidalgo, W., Stashenko, E., Torres, R., & Ortiz, C. (2020). Essential oils of aromatic plants with antibacterial, anti-biofilm and anti-quorum sensing activities against pathogenic bacteria. Antibiotics, 9(4), 147. https://doi.org/10.3390/antibiotics9040147 google scholar
  • Doğan, Ş., Gökalsın, B., Şenkardeş, İ., Doğan, A., & Sesal, N. C. (2019). Antiquorum sensing and anti-biofilm activities of Hypericum perforatum extracts against Pseudomonas aeruginosa. Journal of Ethnopharmacol-ogy, 235, 293-300. https://doi.org/10.1016/j.jep.2019.02.020 google scholar
  • Eris, R., & Ulusoy, S. (2013). Rose, clove, chamomile essential oils and pine turpentine inhibit quorum sensing in Chromobacterium violaceum and Pseudomonas aeruginosa. Journal of Essential Oil Bearing Plants, 16(2), 126-135. https://doi.org/10.1080/097206 0X.2013.794026 google scholar
  • Husain, F. M., Ahmad, I., Khan, M. S., Ahmad, E., Tahseen, Q., Khan, M. S., & Alshabib, N. A. (2015). Sub-MICs of Mentha piperita essen-tial oil and menthol inhibits AHL mediated quorum sensing and biofilm of Gram-negative bacteria. Frontiers in Microbiology, 6, 420. https://doi.org/10.3389/fmicb.2015.00420 google scholar
  • Ilic-TomiĞ, T., Sokovic, M., Vojnovic, S., Ciric, A. D., Veljic, M., Nikodinovic-Runic, J., & Novakovic, M. M. (2017). Diarylheptanoids from Alnus viridis ssp viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa. Planta med-ica, 83(01-02), 117-125. https://doi.org/10.1055/s-0042-107674 Kavanaugh, N. L., & Ribbeck, K. (2012). Selected antimicrobial essential oils eradicate Pseudomonas spp. and Staphylococcus aureus biofilms. Applied and Environmental Microbiology, 78(11), 4057-4061. https://doi.org/10.1128/AEM.07499-11 google scholar
  • Kerekes, E. B., Deak, E., Tako, M., Tserennadmid, R., Petkovits, T., Vagvölgyi, C., & Krisch, J. (2013). Anti-biofilm forming and anti-quorum sensing activity of selected essential oils and their main components on food-related micro-organisms. Journal of Applied Microbiology, 115(4), 933-942. https://doi.org/10.1111/jam.12289 Li, S., Chen, S., Fan, J., Cao, Z., Ouyang, W., Tong, N., Hu, X., Hu, J., Li, P., Feng, Z. & Huang, X. (2018). Anti-biofilm effect of novel thiazole acid analogs against Pseudomonas aeruginosa through IQS path-ways. European Journal of Medicinal Chemistry, 145, 64-73. https:// doi.org/10.1016/j.ejmech.2017.12.076 google scholar
  • Lufs, Â., Duarte, A., Gominho, J., Domingues, F., & Duarte, A. P, (2016). Chemical composition, antioxidant, antibacterial and anti-quorum sensing activities of Eucalyptus globulus and Eucalyptus radiata essential oils. Industrial Crops and Products, 79, 274-282. https://doi.org/10.1016/j.indcrop.2015.10.055 google scholar
  • Merghni, A., Noumi, E., Hadded, O., Dridi, N., Panwar, H., Ceylan, O., Mastouri, M. & Snoussi, M. (2018). Assessment of the antibiofilm and antiquorum sensing activities of Eucalyptus globulus essen-tial oil and its main component 1, 8-cineole against methicillin-resistant Staphylococcus aureus strains. Microbial Pathogenesis, 118, 74-80. https://doi.org/10.1016/j.micpath.2018.03.006 google scholar
  • Mokhetho, K. C., Sandasi, M., Ahmad, A., Kamatou, G. P., & Viljoen, A. M. (2018). Identification of potential anti-quorum sensing com-pounds in essential oils: A gas chromatography-based metabo-lomics approach. Journal of Essential Oil Research, 30(6), 399-408. https://doi.org/10.1080/10412905.2018.1503100 google scholar
  • Mukherji, R., & Prabhune, A. (2014). Novel glycolipids synthesized using plant essential oils and their application in quorum sensing inhibition and as antibiofilm agents. The Scientific World Journal, 2014. https://doi.org/10.1155/2014/890709 google scholar
  • O’Toole, G. A., & Kolter, R. (1998). Initiation of biofilm forma-tion in Pseudomonas fluorescens WCS365 proceeds via mul-tiple, convergent signalling pathways: a genetic analysis. Molecular Microbiology, 28(3), 449-461. https://doi.org/10.1046/ j.1365-2958.1998.00797.x google scholar
  • Oh, S., Yun, W., Lee, J., Lee, C., Kwak, W., & Cho, J. (2017). Effects of es-sential oil (blended and single essential oils) on anti-biofilm forma-tion of Salmonella and Escherichia coli. Journal of Animal Science and Technology, 59(1), 1-5. https://doi.org/10.1186/s40781-017-0127-7 Önem, E., Tüzün, B., & Akkoç, S. (2021). Anti-quorum sensing activ-ity in Pseudomonas aeruginos a PA01 of benzimidazolium salts: electronic, spectral and structural investigations as theoretical approach. Journal of Biomolecular Structure and Dynamics, 1-12. https://doi.org/10.1080/07391102.2021.1890222 google scholar
  • Packiavathy, I. A. S. V., Agilandeswari, P., Musthafa, K. S., Pandian, S. K., & Ravi, A. V. (2012). Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens. Food Research Interna-tional, 45(1), 85-92. https://doi.org/10.1016/j.foodres.2011.10.022 Pellegrini, M. C., Alvarez, M. V., Ponce, A. G., Cugnata, N. M., De Piano, F. G., & Fuselli, S. R. (2014). Anti-quorum sensing and antimi-crobial activity of aromatic species from South America. Journal of Essential Oil Research, 26(6), 458-465. https://doi.org/10.1080/1 0412905.2014.947387 google scholar
  • Raut, J. S., & Karuppayil, S. M. (2014). A status review on the me-dicinal properties of essential oils. Industrial Crops and Products, 62, 250-264. https://doi.org/10.1016/j.indcrop.2014.05.055 google scholar
  • Tariq, S., Wani, S., Rasool, W., Shafi, K., Bhat, M. A., Prabhakar, A., Shalla, A.H. & Rather, M.A. (2019). A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant micro-bial pathogens. Microbial Pathogenesis, 134, 103580.https://doi. org/10.1016/j.micpath.2019.103580 google scholar
  • Vasavi, H. S., Arun, A. B., & Rekha, P. D. (2014). Anti-quorum sensing activity of Psidium guajava L. flavonoids against Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. Microbiology and Im-munology, 58(5), 286-293. https://doi.org/10.1111/1348-0421.12150 Vieira, M., Bessa, L. J., Martins, M. R., Arantes, S., Teixeira, A. P., Mendes, Â., Martins da Costa, P. & Belo, A. D. (2017). Chemical composition, antibacterial, antibiofilm and synergistic proper-ties of essential oils from Eucalyptus globulus Labill. and seven Mediterranean aromatic plants. Chemistry & Biodiversity, 14(6), e1700006. https://doi.org/10.1002/cbdv.201700006 google scholar
  • Vipin, C., Mujeeburahiman, M., Ashwini, P., Arun, A., & Rekha, P. D. (2019). Anti-biofilm and cytoprotective activities of quercetin against Pseudomonas aeruginosa isolates. Letters in Applied Mi-crobiology, 68(5), 464-471. https://doi.org/10.1111/lam.13129 google scholar
  • Yan, M.-R., Wang, C.-H., Cruz Flores, N. J., & Su, Y.-Y. (2019). Targeting open market with strategic business innovations: a case study of growth dynamics in essential oil and aromatherapy industry. Journal of Open Innovation: Technology, Market, and Complexity, 5(1), 7. https://doi.org/10.3390/joitmc5010007 google scholar
  • Yap, P. S. X., Yiap, B. C., Ping, H. C., & Lim, S. H. E. (2014). Essen-tial oils, a new horizon in combating bacterial antibiotic re-sistance. The Open Microbiology Journal, 8, 6. https://doi. org/10.2174/1874285801408010006 google scholar
  • Zahin, M., Hasan, S., Aqil, F., Khan, M., Ahmad, S., Husain, F. M., & Ahmad, I. (2010). Screening of certain medicinal plants from India for their anti-quorum sensing activity. http://hdl.handle.net/123456789/10652 google scholar
  • Zgoda, J., & Porter, J. (2001). A convenient microdilution method for screening natural products against bacteria and fungi. Pharmaceuti-cal Biology, 39(3), 221-225. https://doi.org/10.1076/phbi.39.3.221.5934">https://doi.org/10.1076/phbi.39.3.221.5934 Zgurskaya, H. I., Lopez, C. A., & Gnanakaran, S. (2015). Perme-ability barrier of Gram-negative cell envelopes and approaches to bypass it. ACS Infectious Diseases, 1(11), 512-522. https://doi. org/10.1021/acsinfecdis.5b00097 google scholar
  • Zhang, D., Gan, R. Y., Zhang, J. R., Farha, A. K., Li, H. B., Zhu, F., Wang, X.H. & Corke, H. (2020). Antivirulence properties and related mechanisms of spice essential oils: A comprehensive review. Comprehensive Reviews in Food Science and Food Safety, 19(3), 1018-1055. https://doi.org/10.1111/1541-4337.12549 google scholar
  • Zhang, Y., Djakpo, O., Xie, Y., Guo, Y., Yu, H., Cheng, Y., Qian, H., Shi, R. & Yao, W. (2019). Anti-quorum sensing of Galla chinensis and Coptis chinensis on bacteria. Lebensmittel-Wissenchaft und Tech-nologie, 101, 806-811. https://doi.org/10.1016/j.lwt.2018.11.090 google scholar
  • Zhang, Y., Kong, J., Xie, Y., Guo, Y., Cheng, Y., Qian, H., & Yao, W. (2018). Essential oil components inhibit biofilm formation in Erwinia caroto-vora and Pseudomonas fluorescens via anti-quorum sensing activ-ity. Lebensmittel-Wissenchaft und Technologie, 92, 133-139. https://doi. org/10.1016/j.lwt.2018.02.027 google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Özfenerci, M., Bali, E.B., Erdönmez, D., & Koca Çalışkan, U. (2022). Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens. İstanbul Journal of Pharmacy, 52(1), 54-63. https://doi.org/10.26650/IstanbulJPharm.2022.998774


AMA

Özfenerci M, Bali E B, Erdönmez D, Koca Çalışkan U. Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens. İstanbul Journal of Pharmacy. 2022;52(1):54-63. https://doi.org/10.26650/IstanbulJPharm.2022.998774


ABNT

Özfenerci, M.; Bali, E.B.; Erdönmez, D.; Koca Çalışkan, U. Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 1, p. 54-63, 2022.


Chicago: Author-Date Style

Özfenerci, Müjgan, and Elif Burcu Bali and Demet Erdönmez and Ufuk Koca Çalışkan. 2022. “Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens.” İstanbul Journal of Pharmacy 52, no. 1: 54-63. https://doi.org/10.26650/IstanbulJPharm.2022.998774


Chicago: Humanities Style

Özfenerci, Müjgan, and Elif Burcu Bali and Demet Erdönmez and Ufuk Koca Çalışkan. Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens.” İstanbul Journal of Pharmacy 52, no. 1 (May. 2024): 54-63. https://doi.org/10.26650/IstanbulJPharm.2022.998774


Harvard: Australian Style

Özfenerci, M & Bali, EB & Erdönmez, D & Koca Çalışkan, U 2022, 'Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens', İstanbul Journal of Pharmacy, vol. 52, no. 1, pp. 54-63, viewed 11 May. 2024, https://doi.org/10.26650/IstanbulJPharm.2022.998774


Harvard: Author-Date Style

Özfenerci, M. and Bali, E.B. and Erdönmez, D. and Koca Çalışkan, U. (2022) ‘Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens’, İstanbul Journal of Pharmacy, 52(1), pp. 54-63. https://doi.org/10.26650/IstanbulJPharm.2022.998774 (11 May. 2024).


MLA

Özfenerci, Müjgan, and Elif Burcu Bali and Demet Erdönmez and Ufuk Koca Çalışkan. Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens.” İstanbul Journal of Pharmacy, vol. 52, no. 1, 2022, pp. 54-63. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.998774


Vancouver

Özfenerci M, Bali EB, Erdönmez D, Koca Çalışkan U. Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens. İstanbul Journal of Pharmacy [Internet]. 11 May. 2024 [cited 11 May. 2024];52(1):54-63. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.998774 doi: 10.26650/IstanbulJPharm.2022.998774


ISNAD

Özfenerci, Müjgan - Bali, ElifBurcu - Erdönmez, Demet - Koca Çalışkan, Ufuk. Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens”. İstanbul Journal of Pharmacy 52/1 (May. 2024): 54-63. https://doi.org/10.26650/IstanbulJPharm.2022.998774



TIMELINE


Submitted22.09.2021
Accepted15.12.2021
Published Online28.04.2022

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.