Research Article


DOI :10.26650/IstanbulJPharm.2022.1057224   IUP :10.26650/IstanbulJPharm.2022.1057224    Full Text (PDF)

Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay

Seher KarslıSelma YazarEsra F. İncedere DüzdağTürkan Yurdun

Background and Aims: Pesticide poisoning is the most widespread occupational hazard for agricultural workers in the developing world, due to the extensive presence of pesticides in the environment. The aim of this study was to investigate the cytotoxicity and DNA damaging effects of organophosphosphate and carbamate pesticides. Methods: In the present study, the cytotoxicity of chlorpyrifos methyl, azinphos ethyl, [(O-Ethyl O-(p-nitrophenyl) pheriylphosphonothioate] (EPN), aldicarb sulfone, and ethiofencarb were assessed by the trypan blue dye exclusion method. An alkaline comet assay was performed to assess the genotoxic effects of applied pesticides in human peripheral blood lymphocytes. Results: We demonstrated the cytotoxic effect of EPN following 30 and 120 min exposure at 100 µg/mL concentration. Although chlorpyrifos-methyl and azinphos ethyl seem to be safer concerning cytotoxicity compared to other pesticides, significantly higher DNA damage levels were determined after exposure of these pesticides for 120 min at 100 µg/mL concentration by in vitro comet assay. The potential DNA-damaging effects of these pesticides were sorted from high to low, as chlorpyrifos-methyl, aldicarb sulfone, EPN, and azinphos ethyl after 30 min of exposure, and were sorted as chlorpyrifosmethyl, azinphos ethyl, aldicarb sulfone, and EPN after 120 min of exposure. Our results revealed that these pesticides tend to increase DNA damage in a dose- and time-dependent manner. Conclusion: The genotoxic effects of these widely used pesticides may cause prominent and serious health risks for human populations; hence, the DNA-damaging potential of pesticides can lead to genotoxic risk and adverse health effects like cancer.


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References

  • Abuwarda, H. N., Alashi, S. F., & Sharif, F. A. (2021). Investigating aneuploidy-inducing effect of Nemacur, Rogor, and Dursban in human peripheral blood lymphocyte cultures. Environmental and Molecular Mutagenesis, 62(5), 328-334. doi:10.1002/em.22445 google scholar
  • Ahmed, F. E. (2001). Analyses of pesticides and their metabolites in foods and drinks. TrAC Trends in Analytical Chemistry, 20(11), 649-661. google scholar
  • Al-Samarraie, M. S. J., Karinen, R., Rognum, T., Hasvold, I., Stokke, M. O., & Christophersen, A. S. (2009). Lethal Poisoning with Ethiofen-carb and Ethanol. Journal of Analytical Toxicology, 33(7), 389-392. doi: 10.1093/jat/33.7.389 google scholar
  • Canna-Michaelidou, S., & Nicolaou, A. S. (1996). Evaluation of the genotoxicity potential (by Mutatox test) of ten pesticides found as water pollutants in Cyprus. Science of the Total Environment, 193(1), 27-35. doi:10.1016/s0048-9697(96)05322-3 google scholar
  • Cid, M. G., & Matos, E. (1984). Induction of sister-chromatid ex-changes in cultured human lymphocytes by aldicarb, a carba-mate pesticide. Mutation Research/Genetic Toxicology, 138(2), 175-179. google scholar
  • Collins, A. R. (2014). Measuring oxidative damage to DNA and its repair with the comet assay. Biochimica et Biophysica Acta, 1840(2), 794-800. doi:10.1016/j.bbagen.2013.04.022 google scholar
  • Das, P., Shaik, A. P., & Jamil, K. (2007). Genotoxicity induced by pes-ticide mixtures: in-vitro studies on human peripheral blood lym-phocytes. Toxicology and Industrial Health, 23(8), 449-458. google scholar
  • Ecobichon, D. J. (2001). Pesticide use in developing countries. Toxicology, 160(1-3), 27-33. doi: 10.1016/S0300-483x(00)00452-2 google scholar
  • Gaikwad, A. S., Karunamoorthy, P., Kondhalkar, S. J., Ambikapathy, M., & Beerappa, R. (2015). Assessment of hematological, bio-chemical effects and genotoxicity among pesticide sprayers in grape garden. Journal of Occupational Medicine and Toxicology, 10(1), 11. google scholar
  • Hodgson, E., & Levi, P. E. (1996). Pesticides: an important but un-derused model for the environmental health sciences. Environ-mental Health Perspectives, 104(Suppl 1), 97. google scholar
  • Jamil, K., Shaik, A. P., Mahboob, M., & Krishna, D. (2004). Effect of organophosphorus and organochlorine pesticides (monochro-tophos, chlorpyriphos, dimethoate, and endosulfan) on human lymphocytes in-vitro. Drug& Chemical Toxicology, 27(2), 133-144. doi:10.1081/dct-120030725 google scholar
  • Kaur, P., Radotra, B., Minz, R. W., & Gill, K. (2007). Impaired mito-chondrial energy metabolism and neuronal apoptotic cell death after chronic dichlorvos (OP) exposure in rat brain. Neurotoxicol-ogy, 28(6), 1208-1219. google scholar
  • Kisby, G. E., Muniz, J. F., Scherer, J., Lasarev, M. R., Koshy, M., Kow, Y. W., & McCauley, L. (2009). Oxidative stress and DNA damage in agricultural workers. Journal of Agromedicine, 14(2), 206-214. google scholar
  • McCauley, L. A., Lasarev, M. R., Higgins, G., Rothlein, J., Muniz, J., Ebbert, C., & Phillips, J. (2001). Work characteristics and pesticide exposures among migrant agricultural families: a community-based research approach. Environmental Health Perspectives, 109(5), 533. google scholar
  • McKelvey-Martin, V. J., Green, M. H., Schmezer, P., Pool-Zobel, B. L., De Meo, M. P., & Collins, A. (1993). The single cell gel electropho-resis assay (comet assay): a European review. Mutation Research, 288(1), 47-63. doi:10.1016/0027-5107(93)90207-v google scholar
  • Mehta, A., Verma, R. S., & Srivastava, N. (2008). Chlorpyrifos-in-duced DNA damage in rat liver and brain. Environmental and Mo-lecular Mutagenesis, 49(6), 426-433. google scholar
  • Mohanty, G., Mohanty, J., Jena, S. D., & Dutta, S. (2011). Geno-toxicity Testing in Pesticide Safety Evaluation. In M. Stoytcheva (Ed.) Pesticides in the Modern World-Pests Control and Pesticides Exposure and Toxicity Assessment (pp. 403-426). Rijeka, Croatia: InTech. google scholar
  • Muniz, J. F., McCauley, L., Scherer, J., Lasarev, M., Koshy, M., Kow, Y., . . . Kisby, G. (2008). Biomarkers of oxidative stress and DNA dam-age in agricultural workers: a pilot study. Toxicology and Applied Pharmacology, 227(1), 97-107. google scholar
  • Munoz-Quezada, M. T., Lucero, B. A., Iglesias, V. P., Munoz, M. P., Cornejo, C. A., Achu, E., . . . Villalobos, M. (2016). Chronic exposure to organophosphate (OP) pesticides and neuropsychological functioning in farm workers: a review. International Journal of Oc-cupational and Environmental Health, 22(1), 68-79. doi:10.1080/10 773525.2015.1123848 google scholar
  • Ni, Z., Li, S., Liu, Y., Tang, Y., & Pang, D. (1993). Induction of micro-nucleus by organophosphorus pesticides both in vivo and in vi-tro. Hua xi yi ke da xue xue bao= Journal of West China University of Medical Sciences= Huaxi yike daxue xuebao/[bian ji zhe, Hua xi yi ke da xue xue bao bian wei hui], 24(1), 82-86. google scholar
  • Ojha, A., & Srivastava, N. (2014). In vitro studies on organophos-phate pesticides induced oxidative DNA damage in rat lympho-cytes. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 761, 10-17. google scholar
  • Okonko, L., Ikpeme, E., & Udensi, O. (2016). Genotoxic Effect of Chlorpyrifos and Cypermethrin in Albino Rats. Research Journal of Mutagenesis, 6(1), 31. google scholar
  • Petroianu, G. A., Nurulain, S. M., Hasan, M. Y., Kuca, K., & Lorke, D. E. (2015). Reversible cholinesterase inhibitors as pre-treatment for exposure to organophosphates: assessment using azinphos-methyl. Journal of Applied Toxicology, 35(5), 493-499. doi:10.1002/ jat.3052 google scholar
  • Proença, P., Teixeira, H., De Mendonça, M., Castanheira, F., Marques, E. P., Corte-Real, F., & Vieira, D. N. (2004). Aldicarb poisoning: one case report. Forensic Science International, 146, S79-S81. google scholar
  • Rahman, M., Mahboob, M., Danadevi, K., Banu, B. S., & Grover, P. (2002). Assessment of genotoxic effects of chloropyriphos and acephate by the comet assay in mice leucocytes. Mutation Re-search/Genetic Toxicology and Environmental Mutagenesis, 516(1), 139-147. google scholar
  • Sandal, S., & Yilmaz, B. (2011). Genotoxic effects of chlorpyrifos, cypermethrin, endosulfan and 2, 4-D on human peripheral lym-phocytes cultured from smokers and nonsmokers. Environmental Toxicology, 26(5), 433-442. google scholar
  • Shadnia, S., Azizi, E., Hosseini, R., Khoei, S., Fouladdel, S., Pajou-mand, A., . . . Abdollahi, M. (2005). Evaluation of oxidative stress and genotoxicity in organophosphorus insecticide formulators. Human & Experimental Toxicology, 24(9), 439-445. google scholar
  • Singh, N. P., McCoy, M. T., Tice, R. R., & Schneider, E. L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1), 184-191. doi:10.1016/0014-4827(88)90265-0 google scholar
  • Smith, G. J. (1987). Pesticide Use and Toxicology in Relation to Wildlife: Organophosphorus and Carbamate, Compounds. All U.S. Government Documents (Utah Regional Depository). google scholar
  • Sun, X., Jin, Y., Wu, B., Wang, W., Pang, X., & Wang, J. (2010). Study on genotoxicity of aldicarb and methomyl. Huan jing ke xue= Huanjing kexue, 31(12), 2973-2980. google scholar
  • Suratman, S., Edwards, J. W., & Babina, K. (2015). Organophos-phate pesticides exposure among farmworkers: pathways and risk of adverse health effects. Reviews on Environmental Health, 30(1), 65-79. doi:10.1515/reveh-2014-0072 google scholar
  • Ündeğer, Ü., & Başaran, N. (2002). Assessment of DNA damage in workers occupationally exposed to pesticide mixtures by the alkaline comet assay. Archives of Toxicology, 76(7), 430-436. google scholar
  • van der Werf, H. M. (1996). Assessing the impact of pesticides on the environment. Agriculture, Ecosystems & Environment, 60(2-3), 81-96. google scholar
  • Venkat, J. A., Shami, S., Davis, K., Nayak, M., Plimmer, J. R., Pfeil, R., & Nair, P. P. (1995). Relative genotoxic activities of pesticides evaluated by a modified SOS microplate assay. Environmental and Molecular Mutagenesis, 25(1), 67-76. doi:10.1002/em.2850250110 Vigreux, C., Poul, J., Deslandes, E., Lebailly, P., Godard, T., Sichel, F., . . . Gauduchon, P. (1998). DNA damaging effects of pesticides measured by the single cell gel electrophoresis assay (comet as-say) and the chromosomal aberration test, in CHOK1 cells. Muta-tion Research/Genetic Toxicology and Environmental Mutagenesis, 419(1), 79-90. google scholar

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APA

Karslı, S., Yazar, S., İncedere Düzdağ, E.F., & Yurdun, T. (2022). Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay. İstanbul Journal of Pharmacy, 52(2), 136-142. https://doi.org/10.26650/IstanbulJPharm.2022.1057224


AMA

Karslı S, Yazar S, İncedere Düzdağ E F, Yurdun T. Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay. İstanbul Journal of Pharmacy. 2022;52(2):136-142. https://doi.org/10.26650/IstanbulJPharm.2022.1057224


ABNT

Karslı, S.; Yazar, S.; İncedere Düzdağ, E.F.; Yurdun, T. Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 2, p. 136-142, 2022.


Chicago: Author-Date Style

Karslı, Seher, and Selma Yazar and Esra F. İncedere Düzdağ and Türkan Yurdun. 2022. “Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay.” İstanbul Journal of Pharmacy 52, no. 2: 136-142. https://doi.org/10.26650/IstanbulJPharm.2022.1057224


Chicago: Humanities Style

Karslı, Seher, and Selma Yazar and Esra F. İncedere Düzdağ and Türkan Yurdun. Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay.” İstanbul Journal of Pharmacy 52, no. 2 (May. 2024): 136-142. https://doi.org/10.26650/IstanbulJPharm.2022.1057224


Harvard: Australian Style

Karslı, S & Yazar, S & İncedere Düzdağ, EF & Yurdun, T 2022, 'Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay', İstanbul Journal of Pharmacy, vol. 52, no. 2, pp. 136-142, viewed 18 May. 2024, https://doi.org/10.26650/IstanbulJPharm.2022.1057224


Harvard: Author-Date Style

Karslı, S. and Yazar, S. and İncedere Düzdağ, E.F. and Yurdun, T. (2022) ‘Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay’, İstanbul Journal of Pharmacy, 52(2), pp. 136-142. https://doi.org/10.26650/IstanbulJPharm.2022.1057224 (18 May. 2024).


MLA

Karslı, Seher, and Selma Yazar and Esra F. İncedere Düzdağ and Türkan Yurdun. Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay.” İstanbul Journal of Pharmacy, vol. 52, no. 2, 2022, pp. 136-142. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.1057224


Vancouver

Karslı S, Yazar S, İncedere Düzdağ EF, Yurdun T. Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay. İstanbul Journal of Pharmacy [Internet]. 18 May. 2024 [cited 18 May. 2024];52(2):136-142. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.1057224 doi: 10.26650/IstanbulJPharm.2022.1057224


ISNAD

Karslı, Seher - Yazar, Selma - İncedere Düzdağ, EsraF. - Yurdun, Türkan. Assessment of genotoxic effects of organophosphate and carbamate pesticides by comet assay”. İstanbul Journal of Pharmacy 52/2 (May. 2024): 136-142. https://doi.org/10.26650/IstanbulJPharm.2022.1057224



TIMELINE


Submitted13.01.2022
Accepted27.05.2022
Published Online29.08.2022

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