Research Article


DOI :10.26650/IstanbulJPharm.2019.19020   IUP :10.26650/IstanbulJPharm.2019.19020    Full Text (PDF)

Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line

Ezgi ÖztaşMahmoud AbudayyakBeyza AykanatZübeyde CanEnes BaramGül Özhan

Bismuth (III) oxide nanoparticles’ (Bi2 O3 -NPs) unique physicochemical properties have attracted attention in biological, industrial, technological and medical fields. Concurrently, increasing numbers of studies revealing their potential toxic effects and possible toxicity mechanisms are ongoing. In this study, we assessed the toxic potentials of Bi2 O3 -NPs in human SH-SY5Y neuroblastoma cell line. After Bi2 O3 -NPs characterization using TEM, the cytotoxic potentials were evaluated by MTT and LDH assays. The induction of reactive oxygen species production was evaluated by H2 DCFDA. In order to evaluate the oxidative damages, the changes in antioxidant catalase and superoxide dismutase and glutathione levels were determined. The cellular death pathway and the role of immune response were studied by measuring the mRNA expression levels of related genes. Our results showed that Bi2 O3 -NPs decreased the cell viability through disruption on mitochondrial activity (IC50:77.57 µg/mL) and membrane integrity (LDH%50:16.97 µg/mL). At 50 µg/mL Bi2 O3 -NPs, the production of reactive oxygen species (ROS) was induced significantly as well as the catalase and superoxide dismutase levels. In immune response, the mRNA expression levels of interleukin (IL)-6 increased more than 1.5-fold in all doses; whereas, TNF-α, NF-ĸB and MAPK8 expressions remained unchanged. Consequently, Bi2 O3 -NPs induced oxidative stress-related inflammation via activation of proinflammatory cytokine, IL-6. 


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References

  • Abudayyak M, Öztaş E, Arici M, Özhan G (2017). Investigation of the toxicity of bismuth oxide nanoparticles in various cell lines. Chemosphere 169: 117-123. google scholar
  • Abudayyak M, Guzel EE, Özhan G (2017b). Nickel oxide nanoparticles are highly toxic to SH-SY5Y neuronal cells. Neurochem Int 108: 7-14. google scholar
  • Afanas’ev I (2011). Reactive oxygen species signalling in cancer: comparison with aging. Aging Dis 2: 219-230. google scholar
  • Ahamed M, Akhtar MJ, Khan MAM, Alrokayan SA, Alhadlaq HA (2019) Oxidative stress mediated cytotoxicity and apoptosis response of bismuth oxide (Bi2O3) nanoparticles in human breast cancer (MCF-7) cells. Chemosphere 216: 823-831. google scholar
  • Akbarzadeh F, Khoshgard K, Hosseinzadeh L, Arkan E, Rezazadeh D (2018) Investigating the Cytotoxicity of Folate-Conjugated Bismuth Oxide Nanoparticles on KB and A549 Cell Lines. Adv Pharm Bull 8: 627-635. google scholar
  • Arora S, Rajwade JM, Paknikar KM (2012). Nanotoxicology and in vitro studies: the need of the hour. Toxicol Appl Pharmacol 258: 151-165. google scholar
  • Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254. google scholar
  • Bogusza K, Teheib M, Cardilloa D, Lerchb M, Rosenfeldb A, Doua SX, Liua HK, Konstantino K (2018) High toxicity of Bi(OH)3 and α-Bi2O3 nanoparticles towards malignant 9L and MCF-7 cells. Mater Sci Eng C 93: 958-967. google scholar
  • Chen 1 J, Zhu 1J, Cho, H H, Cui K, Li F, Zhou, Rogers JT, Wong SCT, Huang X (2008). Differential cytotoxicity of metal oxide nanoparticles. J Exp Nanosci 3: 321-328. google scholar
  • Chandra J, Samali A, Orrenius S (2000). Triggering and modulation of apoptosis by oxidative stress. Free Radic Biol Med 29: 323-333. google scholar
  • Choi AO, Cho SJ, Desbarats J, Lovrić J, Maysinger D (2007). Quantum dot-induced cell death involves Fas upregulation and lipid peroxidation in human neuroblastoma cells. J Nanobiotechnol 5: 1. google scholar
  • Cornélio ALG, Salles LP, da Paz MC, Cirelli JA, Guerreiro-Tanomaru JM, Tanomaru Filho M (2011). Cytotoxicity of Portland cement with different radiopacifying agents: a cell death study. J Endod 37: 203-210. google scholar
  • Dhawan A, Sharma V (2010). Toxicity assessment of nanomaterials: methods and challenges. Anal Bioanal Chem 398: 589-605. google scholar
  • Elsaesser A, Howard CV (2012). Toxicology of nanoparticles. Adv Drug Deliv Rev 64: 129-137. google scholar
  • EPA, U.S. (2007). EPA Nanotechnology White Paper. U.S. Environmental Protection Agency, Washington, USA. google scholar
  • Eruslanov E, Kusmartsev S (2010). Identification of ROS using oxidized DCFDA and flow-cytometry. In: Advanced protocols in oxidative stress II. Armstrong, Donald (Ed.) 57-72. google scholar
  • Fotakis G, Timbrell JA (2006). In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 160: 171-177. google scholar
  • Geyikoglu F, Turkez H (2005). Genotoxicity and oxidative stress induced by some bismuth compounds in human blood cells in vitro. Fresenius Environ Bul 14: 854e860. google scholar
  • Han X, Gelein R, Corson N, Wade-Mercer P, Jiang J, Biswas P, Finkelstein JN, Elder A, Oberdörster G (2011). Validation of an LDH assay for assessing nanoparticle toxicity. Toxicology 287: 99-104. google scholar
  • Hernandez-Delgadillo R, Velasco-Arias D, Martinez-Sanmiguel JJ, Diaz D, Zumeta-Dube I, Arevalo-Niño K, Cabral-Romero C (2013). Bismuth oxide aqueous colloidal nanoparticles inhibit Candida albicans growth and biofilm formation. Int J Nanomedicine 8: 1645-1652. google scholar
  • Hyodo T, Kanazawa E, Takao Y, Shimizu Y, Egashira M (2000). H-2 sensing properties and mechanism of Nb2O5-Bi2O3 varistor-type gas sensors. Electrochemistry 68: 24-31. google scholar
  • Iavicoli I, Fontana L, Leso V, Bergamaschi A (2013). The effects of nanomaterials as endocrine disruptors. Int J Mol Sci 14: 16732-16801. google scholar
  • Kim YJ, Yu M, Park HO, Yang SI (2010). Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by silica nanomaterials in human neuronal cell line. Mol Cell Toxicol 6: 336-343. google scholar
  • Liman R (2013). Genotoxic effects of Bismuth (III) oxide nanoparticles by Allium and Comet assay. Chemosphere 93: 269-273. google scholar
  • Love SA, Maurer-Jones MA, Thompson JW, Lin YS, Haynes CL (2012). Assessing nanoparticle toxicity. Annu Rev Anal Chem 5: 181-205. google scholar
  • Marklov E (2007). Inflammation and genes. Acta Medica-Hradec Kralove 50: 17-21. google scholar
  • Maynard AD, Aitken RJ, Butz T, Colvin V, Donaldson K, Oberdörster G, Philbert MA, Ryan J, Seaton A, Stone, V, Tinkle SS, Tran L, Walker NJ, Warheit DB (2006). Safe handling of nanotechnology. Nature 444: 267-269. google scholar
  • Mnyusiwalla A, Daar AS, Singer PA (2003). ‘Mind the gap’: science and ethics in nanotechnology. Nanotechnology 14: R9. google scholar
  • Monteiller C, Tran L, MacNee W, Faux SP, Jones AD, Miller BG, Donaldson K (2007). The pro-inflammatory effects of low solubility low toxicity particles, nanoparticles and fine particles, on epithelial cells in vitro: The role of surface area. Occup Environ Med 64: 609-615. google scholar
  • Niwa Y, Hiura Y, Sawamura H, Iwai N (2008). Inhalation exposure to carbon black induces inflammatory response in rats. Circulation 72: 144-149. google scholar
  • Oztas E, Abudayyak M, Celiksoz M, Özhan G. (2019). Inflammation and oxidative stress are key mediators in AKB48-induced neurotoxicity in vitro. Toxicol in Vitro 55: 101-107. google scholar
  • Park EJ, Park K (2009). Oxidative stress and pro-inflammatory responses induced by silica nanoparticles in vivo and in vitro. Toxicol Lett 184: 18-25. google scholar
  • Rabin O, Perez JM, Grimm J, Wojtkiewicz G, Weissleder R (2006). An X-ray computed tomography imaging agent based on long-circulating bismuth sulphide nanoparticles. Nat Mater 5: 118-122. google scholar
  • Rao KMK, Porter DW, Meighan T, Castranova V (2004). The sources of inflammatory mediators in the lung after silica exposure. Environ Health Perspec 112: 1679-1685. google scholar
  • Ray PC, Yu H, Fu PP (2009). Toxicity and environmental risks of nanomaterials: challenges and future needs. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 27: 1-35. google scholar
  • Schrand AM, Rahman MF, Hussain SM, Schlager JJ, Smith DA, Syed AF (2010). Metal-based nanoparticles and their toxicity assessment. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2: 544-568. google scholar
  • Seabra AB, Durán N (2015). Nanotoxicology of metal oxide nanoparticles. Metals 5: 934-975. google scholar
  • Shishodia S, Aggarwal BB (2002). Nuclear factor-κB activation: a question of life or death. BMB Rep 35: 28-40. google scholar
  • Sorg O (2004). Oxidative stress: a theoretical model or a biological reality? C R Biol 327: 649-662. google scholar
  • Song Q, Liu Y, Jiang Z, Tang M, Li N, Wei F, Cheng G (2014) The acute cytotoxicity of bismuth ferrite nanoparticles on PC12 cells. J Nanopart Res 16: 2408. google scholar
  • Taufik S, Yusof NA, Tee TW, Ramli I (2011). Bismuth oxide nanoparticles/chitosan/modified electrode as biosensor for DNA hybridization. Int J Electrochem Sci 6: 1880-1891. google scholar
  • Thomas F, Bialek B, Hensel R (2011). Medical use of bismuth: the two sides of the coin. J Clin Toxicol S, 3, 2161-0495. google scholar
  • Van Meerloo J, Kaspers GJ, Cloos J (2011). Cell sensitivity assays: the MTT assay. Methods Mol Biol 237-245. google scholar
  • Vance M E, Kuiken T, Vejerano EP, McGinnis SP, Hochella Jr, MF, Rejeski D, Hull MS (2015). Nanotechnology in the real world: Redeveloping the nanomaterial consumer products inventory. Beilstein J Nanotech 6: 1769-1780. google scholar
  • Xie HR, Hu LS, Li GY (2010). SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons in Parkinson’s disease. Chin Med J 123: 1086-1092. google scholar

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APA

Öztaş, E., Abudayyak, M., Aykanat, B., Can, Z., Baram, E., & Özhan, G. (2019). Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line. İstanbul Journal of Pharmacy, 49(3), 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020


AMA

Öztaş E, Abudayyak M, Aykanat B, Can Z, Baram E, Özhan G. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line. İstanbul Journal of Pharmacy. 2019;49(3):173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020


ABNT

Öztaş, E.; Abudayyak, M.; Aykanat, B.; Can, Z.; Baram, E.; Özhan, G. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line. İstanbul Journal of Pharmacy, [Publisher Location], v. 49, n. 3, p. 173-179, 2019.


Chicago: Author-Date Style

Öztaş, Ezgi, and Mahmoud Abudayyak and Beyza Aykanat and Zübeyde Can and Enes Baram and Gül Özhan. 2019. “Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line.” İstanbul Journal of Pharmacy 49, no. 3: 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020


Chicago: Humanities Style

Öztaş, Ezgi, and Mahmoud Abudayyak and Beyza Aykanat and Zübeyde Can and Enes Baram and Gül Özhan. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line.” İstanbul Journal of Pharmacy 49, no. 3 (May. 2024): 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020


Harvard: Australian Style

Öztaş, E & Abudayyak, M & Aykanat, B & Can, Z & Baram, E & Özhan, G 2019, 'Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line', İstanbul Journal of Pharmacy, vol. 49, no. 3, pp. 173-179, viewed 9 May. 2024, https://doi.org/10.26650/IstanbulJPharm.2019.19020


Harvard: Author-Date Style

Öztaş, E. and Abudayyak, M. and Aykanat, B. and Can, Z. and Baram, E. and Özhan, G. (2019) ‘Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line’, İstanbul Journal of Pharmacy, 49(3), pp. 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020 (9 May. 2024).


MLA

Öztaş, Ezgi, and Mahmoud Abudayyak and Beyza Aykanat and Zübeyde Can and Enes Baram and Gül Özhan. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line.” İstanbul Journal of Pharmacy, vol. 49, no. 3, 2019, pp. 173-179. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.19020


Vancouver

Öztaş E, Abudayyak M, Aykanat B, Can Z, Baram E, Özhan G. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line. İstanbul Journal of Pharmacy [Internet]. 9 May. 2024 [cited 9 May. 2024];49(3):173-179. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.19020 doi: 10.26650/IstanbulJPharm.2019.19020


ISNAD

Öztaş, Ezgi - Abudayyak, Mahmoud - Aykanat, Beyza - Can, Zübeyde - Baram, Enes - Özhan, Gül. Bismuth oxide nanoparticles induced oxidative stress-related inflammation in SH-SY5Y cell line”. İstanbul Journal of Pharmacy 49/3 (May. 2024): 173-179. https://doi.org/10.26650/IstanbulJPharm.2019.19020



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Accepted20.09.2019

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