İstanbul Journal of Pharmacy

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DOI :10.26650/IstanbulJPharm.2022.1057338   IUP :10.26650/IstanbulJPharm.2022.1057338    Full Text (PDF)

Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines

Belkıs Atasever Arslan, Kaan Yılancıoğlu, Seda Kuşoğlu Gültekin, İrem Gülfem Albayrak

Background and Aims: This study was aimed to investigate the anti-cancer effect of Isochrysis galbana microalgae extract. Methods: In the study, the chemical composition of Isochrysis galbana microalgae extract was analyzed, and its cytotoxic effect against K562, HL60, U937, MOLT-4, and Raji cancer cells was investigated. ECV304 endothelial cells were used as a healthy cell line for the understanding of its selective cytotoxicity. To determine the effects of Isochrysis galbana extract, an MTT cytotoxicity assay was performed. Results: According to the results of the experiments, the highest cytotoxic effect of Isochrysis galbana microalgae extract was shown at about 24.07±6.48% cytotoxicity against Raji cells. There were a large number of bioactive molecules in the extract, and these molecules showed a specific response to Raji cells when considering the synergistic and antagonistic effects of these molecules on each other. Conclusion: According to the results of GC-MS analysis of Isochrysis galbana microalgae extract, the most intense molecules in the content were Dodecanoic acid, 3-hydroxy- (CAS) Beta-Hydroxy Dodecanoic Acid, and Propanoic acid, 2-methyl-, 1-(1,1-dimethylethyl)-2-methyl-1,3-isonide. The investigation of the effect of these molecules specifically against Raji cells is important to determine the possible anti-leukemic molecules and their combinations that show cytotoxicity against this cell line.

Keywords: Microalgae, Cancer, Cytotoxicity, GC-MS

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APA

Atasever Arslan, B., Yılancıoğlu, K., Kuşoğlu Gültekin, S., & Albayrak, İ.G. (2022). Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. İstanbul Journal of Pharmacy, 52(1), 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338


AMA

Atasever Arslan B, Yılancıoğlu K, Kuşoğlu Gültekin S, Albayrak İ G. Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. İstanbul Journal of Pharmacy. 2022;52(1):64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338


ABNT

Atasever Arslan, B.; Yılancıoğlu, K.; Kuşoğlu Gültekin, S.; Albayrak, İ.G. Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 1, p. 64-68, 2022.


Chicago: Author-Date Style

Atasever Arslan, Belkıs, and Kaan Yılancıoğlu and Seda Kuşoğlu Gültekin and İrem Gülfem Albayrak. 2022. “Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines.” İstanbul Journal of Pharmacy 52, no. 1: 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338


Chicago: Humanities Style

Atasever Arslan, Belkıs, and Kaan Yılancıoğlu and Seda Kuşoğlu Gültekin and İrem Gülfem Albayrak. “Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines.” İstanbul Journal of Pharmacy 52, no. 1 (May. 2024): 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338


Harvard: Australian Style

Atasever Arslan, B & Yılancıoğlu, K & Kuşoğlu Gültekin, S & Albayrak, İG 2022, 'Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines', İstanbul Journal of Pharmacy, vol. 52, no. 1, pp. 64-68, viewed 12 May. 2024, https://doi.org/10.26650/IstanbulJPharm.2022.1057338


Harvard: Author-Date Style

Atasever Arslan, B. and Yılancıoğlu, K. and Kuşoğlu Gültekin, S. and Albayrak, İ.G. (2022) ‘Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines’, İstanbul Journal of Pharmacy, 52(1), pp. 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338 (12 May. 2024).


MLA

Atasever Arslan, Belkıs, and Kaan Yılancıoğlu and Seda Kuşoğlu Gültekin and İrem Gülfem Albayrak. “Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines.” İstanbul Journal of Pharmacy, vol. 52, no. 1, 2022, pp. 64-68. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.1057338


Vancouver

Atasever Arslan B, Yılancıoğlu K, Kuşoğlu Gültekin S, Albayrak İG. Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. İstanbul Journal of Pharmacy [Internet]. 12 May. 2024 [cited 12 May. 2024];52(1):64-68. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.1057338 doi: 10.26650/IstanbulJPharm.2022.1057338


ISNAD

Atasever Arslan, Belkıs - Yılancıoğlu, Kaan - Kuşoğlu Gültekin, Seda - Albayrak, İremGülfem. “Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines”. İstanbul Journal of Pharmacy 52/1 (May. 2024): 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.1057338



TIMELINE


Submitted13.01.2022
Accepted09.03.2022
Published Online28.04.2022

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