İstanbul Journal of Pharmacy

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Research Article


DOI :10.26650/IstanbulJPharm.2021.846741   IUP :10.26650/IstanbulJPharm.2021.846741    Full Text (PDF)

Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach

Zeynep Doğan, Yasin Genç, Ümmühan Şebnem Harput, Asuman Karadeniz Pekgöz, İclal Saraçoğlu

Background and Aims: Genus Veronica (Plantaginaceae) is represented by 79 species in Turkish flora, 26 of which are endemic. Veronica species have a variety of uses including diuretic, anticancer, and rheumatic pains, wounds, and respiratory problems. According to phytochemical studies, Veronica species contain predominantly iridoid glucosides with some phenylethanoid and flavonoid glycosides. Methods: The aqueous extract of Veronica peduncularis M.Bieb. was tested for its cytotoxic activity on human rhabdomyosarcoma (RD) and human epidermoid carcinoma (HEp-2) cell lines using the MTT method. Chemical profile of the extract was determined by HPLC-DAD, and isolation studies were conducted. Results: The extract was found to show concentration-dependent cytotoxicity against tested cell lines. In addition, a comparison of the iridoid fraction of Veronica peduncularis with previously isolated iridoid glucosides on the HPLC-DAD system, showed the presence of aucubin, amphicoside, veratroyl catalpol, and veronicoside in this fraction. On the other hand, isolation and structure elucidation of plantamajoside and 4'-O-methylisoscutellarein-7-O-2''-O-(6'''-O-acetyl-ß-D-allopyranosyl)- ß-D-glucopyranoside from the phenolic fractions were performed by serial chromatographic and spectroscopic methods. Conclusion: To the best of our knowledge, this is the first cytotoxic activity and phytochemical study on the titled plant. The presence of iridoid glucosides and 8-hydroxyflavone glycosides is important for the chemotaxonomy of the genus Veronica.

Keywords: 4'-O-methylisoscutellarein-7-O-2''-O-(6'''-O-acetyl-ß-D-allopyranosyl)-ß-D-glucopyranoside, HPLC, iridoid glucosides, phenolics, plantamajoside, Veronica

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APA

Doğan, Z., Genç, Y., Harput, Ü.Ş., Karadeniz Pekgöz, A., & Saraçoğlu, İ. (2021). Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach. İstanbul Journal of Pharmacy, 51(3), 372-377. https://doi.org/10.26650/IstanbulJPharm.2021.846741


AMA

Doğan Z, Genç Y, Harput Ü Ş, Karadeniz Pekgöz A, Saraçoğlu İ. Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach. İstanbul Journal of Pharmacy. 2021;51(3):372-377. https://doi.org/10.26650/IstanbulJPharm.2021.846741


ABNT

Doğan, Z.; Genç, Y.; Harput, Ü.Ş.; Karadeniz Pekgöz, A.; Saraçoğlu, İ. Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach. İstanbul Journal of Pharmacy, [Publisher Location], v. 51, n. 3, p. 372-377, 2021.


Chicago: Author-Date Style

Doğan, Zeynep, and Yasin Genç and Ümmühan Şebnem Harput and Asuman Karadeniz Pekgöz and İclal Saraçoğlu. 2021. “Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach.” İstanbul Journal of Pharmacy 51, no. 3: 372-377. https://doi.org/10.26650/IstanbulJPharm.2021.846741


Chicago: Humanities Style

Doğan, Zeynep, and Yasin Genç and Ümmühan Şebnem Harput and Asuman Karadeniz Pekgöz and İclal Saraçoğlu. “Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach.” İstanbul Journal of Pharmacy 51, no. 3 (Jul. 2024): 372-377. https://doi.org/10.26650/IstanbulJPharm.2021.846741


Harvard: Australian Style

Doğan, Z & Genç, Y & Harput, ÜŞ & Karadeniz Pekgöz, A & Saraçoğlu, İ 2021, 'Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach', İstanbul Journal of Pharmacy, vol. 51, no. 3, pp. 372-377, viewed 2 Jul. 2024, https://doi.org/10.26650/IstanbulJPharm.2021.846741


Harvard: Author-Date Style

Doğan, Z. and Genç, Y. and Harput, Ü.Ş. and Karadeniz Pekgöz, A. and Saraçoğlu, İ. (2021) ‘Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach’, İstanbul Journal of Pharmacy, 51(3), pp. 372-377. https://doi.org/10.26650/IstanbulJPharm.2021.846741 (2 Jul. 2024).


MLA

Doğan, Zeynep, and Yasin Genç and Ümmühan Şebnem Harput and Asuman Karadeniz Pekgöz and İclal Saraçoğlu. “Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach.” İstanbul Journal of Pharmacy, vol. 51, no. 3, 2021, pp. 372-377. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2021.846741


Vancouver

Doğan Z, Genç Y, Harput ÜŞ, Karadeniz Pekgöz A, Saraçoğlu İ. Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach. İstanbul Journal of Pharmacy [Internet]. 2 Jul. 2024 [cited 2 Jul. 2024];51(3):372-377. Available from: https://doi.org/10.26650/IstanbulJPharm.2021.846741 doi: 10.26650/IstanbulJPharm.2021.846741


ISNAD

Doğan, Zeynep - Genç, Yasin - Harput, ÜmmühanŞebnem - Karadeniz Pekgöz, Asuman - Saraçoğlu, İclal. “Chemical profiling and cytotoxic activity of aqueous extract of Veronica peduncularis M.Bieb.: A chemotaxonomical approach”. İstanbul Journal of Pharmacy 51/3 (Jul. 2024): 372-377. https://doi.org/10.26650/IstanbulJPharm.2021.846741



TIMELINE


Submitted25.12.2020
Accepted16.09.2021
Published Online30.12.2021

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