İstanbul Journal of Pharmacy

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DOI :10.26650/IstanbulJPharm.2024.1467008   IUP :10.26650/IstanbulJPharm.2024.1467008    Full Text (PDF)

Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy

Batuhan Yağmur, Şura Akat Pişkin, Erkan Civelek, Deniz Kaleli Durman, Nihan Çarçak Yılmaz

Background and Aims: Anatolian propolis, which is rich in phenolic compounds, may offer neuroprotective benefits due to its anti-inflammatory and antioxidant properties. This study explored how Anatolian propolis affects the frequency of absence seizures and anxiety levels in rats with genetic absence epilepsy from Strasbourg (GAERS).

Methods: Adult male GAERS were orally administered Anatolian propolis samples at concentrations of 15% (120 mg/kg/day) and 30% (180 mg/kg/day), whereas the control group received an equivalent volume of tap water by oral gavage for 35 days. A 3-h EEG was recorded 9.00 and 12.00 a.m after 35 days of sub-chronic administration. The effects of Anatolian propolis on spike-and-wave discharge (SWD) duration, number, and mean duration of each SWDs were evaluated and compared with the control group. The elevated plus maze test was then performed to measure the anxiety level of GAERS rats. Finally, brains were isolated, and interleukin-1 beta (IL-1β) levels were measured in freshly frozen isolated brains using an ELISA method.

Results: Oral administration of Anatolian propolis (180 mg/kg/day) significantly reduced the number of SWDs and decreased IL-1β levels in the brain tissue of adult GAERS after 35 days of sub-chronic administration (p<0.05). Propolis treatment did not alter anxiety levels in terms of time spent in the closed and open arms.

Conclusion: This study represents an initial exploration of the effects of Anatolian propolis on absence seizures in GAERS. Our findings indicate that Anatolian propolis could offer therapeutic advantages by reducing the levels of the brain’s pro-inflammatory cytokine IL-1β in GAERS, potentially mitigating absence seizures. However, additional research is necessary to understand the potential mechanisms driving this benefit.

Keywords: Absence epilepsy, Anatolian Propolis, Anxiety, GAERS, IL-1β, Neuroinflammation

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APA

Yağmur, B., Akat Pişkin, Ş., Civelek, E., Kaleli Durman, D., & Çarçak Yılmaz, N. (2024). Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy. İstanbul Journal of Pharmacy, 54(3), 378-385. https://doi.org/10.26650/IstanbulJPharm.2024.1467008


AMA

Yağmur B, Akat Pişkin Ş, Civelek E, Kaleli Durman D, Çarçak Yılmaz N. Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy. İstanbul Journal of Pharmacy. 2024;54(3):378-385. https://doi.org/10.26650/IstanbulJPharm.2024.1467008


ABNT

Yağmur, B.; Akat Pişkin, Ş.; Civelek, E.; Kaleli Durman, D.; Çarçak Yılmaz, N. Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy. İstanbul Journal of Pharmacy, [Publisher Location], v. 54, n. 3, p. 378-385, 2024.


Chicago: Author-Date Style

Yağmur, Batuhan, and Şura Akat Pişkin and Erkan Civelek and Deniz Kaleli Durman and Nihan Çarçak Yılmaz. 2024. “Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy.” İstanbul Journal of Pharmacy 54, no. 3: 378-385. https://doi.org/10.26650/IstanbulJPharm.2024.1467008


Chicago: Humanities Style

Yağmur, Batuhan, and Şura Akat Pişkin and Erkan Civelek and Deniz Kaleli Durman and Nihan Çarçak Yılmaz. “Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy.” İstanbul Journal of Pharmacy 54, no. 3 (Mar. 2025): 378-385. https://doi.org/10.26650/IstanbulJPharm.2024.1467008


Harvard: Australian Style

Yağmur, B & Akat Pişkin, Ş & Civelek, E & Kaleli Durman, D & Çarçak Yılmaz, N 2024, 'Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy', İstanbul Journal of Pharmacy, vol. 54, no. 3, pp. 378-385, viewed 14 Mar. 2025, https://doi.org/10.26650/IstanbulJPharm.2024.1467008


Harvard: Author-Date Style

Yağmur, B. and Akat Pişkin, Ş. and Civelek, E. and Kaleli Durman, D. and Çarçak Yılmaz, N. (2024) ‘Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy’, İstanbul Journal of Pharmacy, 54(3), pp. 378-385. https://doi.org/10.26650/IstanbulJPharm.2024.1467008 (14 Mar. 2025).


MLA

Yağmur, Batuhan, and Şura Akat Pişkin and Erkan Civelek and Deniz Kaleli Durman and Nihan Çarçak Yılmaz. “Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy.” İstanbul Journal of Pharmacy, vol. 54, no. 3, 2024, pp. 378-385. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2024.1467008


Vancouver

Yağmur B, Akat Pişkin Ş, Civelek E, Kaleli Durman D, Çarçak Yılmaz N. Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy. İstanbul Journal of Pharmacy [Internet]. 14 Mar. 2025 [cited 14 Mar. 2025];54(3):378-385. Available from: https://doi.org/10.26650/IstanbulJPharm.2024.1467008 doi: 10.26650/IstanbulJPharm.2024.1467008


ISNAD

Yağmur, Batuhan - Akat Pişkin, Şura - Civelek, Erkan - Kaleli Durman, Deniz - Çarçak Yılmaz, Nihan. “Effects of Anatolian propolis on absence seizures and anxiety in rats with genetic absence epilepsy”. İstanbul Journal of Pharmacy 54/3 (Mar. 2025): 378-385. https://doi.org/10.26650/IstanbulJPharm.2024.1467008



TIMELINE


Submitted09.04.2024
Accepted29.06.2024
Published Online30.12.2024

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