İstanbul Journal of Pharmacy

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Research Article


DOI :10.26650/IstanbulJPharm.2024.1396798   IUP :10.26650/IstanbulJPharm.2024.1396798    Full Text (PDF)

Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples

Ebrar Şeyma Özdemir, Sümeyye Koç, Demet Özçelik, Selman Aktaş, Mehmet Murat Yekrek, Esra Paydaş Hataysal, Mevlüt Sait Keleş, Fatih Gültekin, Eray Metin Güler

Background and Aims: In vitro and in vivo studies have shown that Zingiber officinale (Z. officinale, Ginger) may have an anticoagulant effect. Although there are studies on the anticoagulant effect, the results are inconclusive. Our study investigated the anticoagulant effect of Z. officinale on the whole blood sample ex vivo.

Methods: The inner and shell parts of Z. officinale were extracted with methanol, ethanol, and water. 0.1 mg/mL of different volumes (100, 150 and 200 μL) of Z. officinale extracts were added to the blood of a healthy volunteer ex vivo. Prothrombin time (PT) and activated partial thromboplastin time (aPTT) were measured in the coagulation analyser. Measurements were performed twice, before and after the ginger treatment. International Normalised Ratio (INR) values were calculated using the following mathematical formula: INR = Patient PT/Control PT. The IBM SPSS 25.0 software was used for statistical analyses.

Results: A notable prolongation in PT, aPTT, and INR was detected after the addition of Z. officinale extract to blood samples (p<0.001). As the volume of Z. officinale extract added to the blood sample increased, coagulation parameters were observed to display a corresponding increase (p<0.001).

Conclusion: Z. officinale was associated with prolonged PT, aPTT, and INR ex vivo. In vivo studies are needed to demonstrate the mechanism of the anticoagulant effect.


Keywords: Activated partial thromboplastin time, Anticoagulant herbs, Ginger, Prothrombin time, Zingiber officinale

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APA

Özdemir, E.Ş., Koç, S., Özçelik, D., Aktaş, S., Yekrek, M.M., Paydaş Hataysal, E., Keleş, M.S., Gültekin, F., & Güler, E.M. (2024). Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples. İstanbul Journal of Pharmacy, 54(3), 417-423. https://doi.org/10.26650/IstanbulJPharm.2024.1396798


AMA

Özdemir E Ş, Koç S, Özçelik D, Aktaş S, Yekrek M M, Paydaş Hataysal E, Keleş M S, Gültekin F, Güler E M. Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples. İstanbul Journal of Pharmacy. 2024;54(3):417-423. https://doi.org/10.26650/IstanbulJPharm.2024.1396798


ABNT

Özdemir, E.Ş.; Koç, S.; Özçelik, D.; Aktaş, S.; Yekrek, M.M.; Paydaş Hataysal, E.; Keleş, M.S.; Gültekin, F.; Güler, E.M. Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples. İstanbul Journal of Pharmacy, [Publisher Location], v. 54, n. 3, p. 417-423, 2024.


Chicago: Author-Date Style

Özdemir, Ebrar Şeyma, and Sümeyye Koç and Demet Özçelik and Selman Aktaş and Mehmet Murat Yekrek and Esra Paydaş Hataysal and Mevlüt Sait Keleş and Fatih Gültekin and Eray Metin Güler. 2024. “Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples.” İstanbul Journal of Pharmacy 54, no. 3: 417-423. https://doi.org/10.26650/IstanbulJPharm.2024.1396798


Chicago: Humanities Style

Özdemir, Ebrar Şeyma, and Sümeyye Koç and Demet Özçelik and Selman Aktaş and Mehmet Murat Yekrek and Esra Paydaş Hataysal and Mevlüt Sait Keleş and Fatih Gültekin and Eray Metin Güler. “Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples.” İstanbul Journal of Pharmacy 54, no. 3 (Mar. 2025): 417-423. https://doi.org/10.26650/IstanbulJPharm.2024.1396798


Harvard: Australian Style

Özdemir, EŞ & Koç, S & Özçelik, D & Aktaş, S & Yekrek, MM & Paydaş Hataysal, E & Keleş, MS & Gültekin, F & Güler, EM 2024, 'Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples', İstanbul Journal of Pharmacy, vol. 54, no. 3, pp. 417-423, viewed 14 Mar. 2025, https://doi.org/10.26650/IstanbulJPharm.2024.1396798


Harvard: Author-Date Style

Özdemir, E.Ş. and Koç, S. and Özçelik, D. and Aktaş, S. and Yekrek, M.M. and Paydaş Hataysal, E. and Keleş, M.S. and Gültekin, F. and Güler, E.M. (2024) ‘Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples’, İstanbul Journal of Pharmacy, 54(3), pp. 417-423. https://doi.org/10.26650/IstanbulJPharm.2024.1396798 (14 Mar. 2025).


MLA

Özdemir, Ebrar Şeyma, and Sümeyye Koç and Demet Özçelik and Selman Aktaş and Mehmet Murat Yekrek and Esra Paydaş Hataysal and Mevlüt Sait Keleş and Fatih Gültekin and Eray Metin Güler. “Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples.” İstanbul Journal of Pharmacy, vol. 54, no. 3, 2024, pp. 417-423. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2024.1396798


Vancouver

Özdemir EŞ, Koç S, Özçelik D, Aktaş S, Yekrek MM, Paydaş Hataysal E, Keleş MS, Gültekin F, Güler EM. Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples. İstanbul Journal of Pharmacy [Internet]. 14 Mar. 2025 [cited 14 Mar. 2025];54(3):417-423. Available from: https://doi.org/10.26650/IstanbulJPharm.2024.1396798 doi: 10.26650/IstanbulJPharm.2024.1396798


ISNAD

Özdemir, EbrarŞeyma - Koç, Sümeyye - Özçelik, Demet - Aktaş, Selman - Yekrek, MehmetMurat - Paydaş Hataysal, Esra - Keleş, MevlütSait - Gültekin, Fatih - Güler, ErayMetin. “Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples”. İstanbul Journal of Pharmacy 54/3 (Mar. 2025): 417-423. https://doi.org/10.26650/IstanbulJPharm.2024.1396798



TIMELINE


Submitted27.11.2023
Accepted21.08.2024
Published Online30.12.2024

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