Research Article


DOI :10.26650/IstanbulJPharm.2019.19035   IUP :10.26650/IstanbulJPharm.2019.19035    Full Text (PDF)

Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract

Mehmet Evren OkurNihal KarakaşAyşe Esra KaradağDamla UludağDerya Çiçek Polat

Opuntia ficus-indica fruits are claimed to possess several health-beneficial properties. This study was carried out to determine the phytochemicals as well as to compare antioxidant activity and cell viability of the methanol extract of OFI-fruits. Antioxidant activity was evaluated by various in vitro tests such as DPPH and ABTS. LC-MS and HPLC analyses were then performed. The activity of the OFI-fruit extract on viability of U87-MG (glioblastoma multiform), HT-29 (colon cancer) and human primary fibroblast cells was investigated by determining viable cells on a luciferase based system (Cell TiterGlo; Promega; USA). The results showed that OFI-fruits were a source of antioxidant compounds. Moreover, the extract was found to have rich phenolic-flavonoid contents. Quercetin was found in the extract according to LC-MS and HPLC studies. A remarkable increased cell death in the HT-29 and U87 cell lines was detected in a concentration-dependent manner (1-10mg/mL). Among these cell lines, HT-29 responded to OFI-fruit treatment more sensitively to even the lowest dose (1mg/mL). The results of this study showed that the OFI-fruit has significant antioxidant capacity and leads to strong cytotoxic activity on various cancer cells. Therefore, it can be suggested that OFI-fruits may have chemo-preventive potential and can be studied further to clarify cytotoxic abilities.


PDF View

References

  • Abdel-Hady H, Mohamed El-Sayed M, Mohamed Abdel-Gawad M, Ahmed El-Wakil E, Saleh Abdel-Hameed E-S, El-Sayed Abdel-Lateef E (2000). LC-ESI-MS analysis, antitumor and antioxidant activities of methanolic extract of Egyptian Allium kurrat. J Appl Pharm Sci 8: 85-92. google scholar
  • Ahmad MS, Ahmad S, Arshad M, Rai KB, Afzal M (2014). Terminalia catappa, an anticlastogenic agent against MMS induced genotoxicity in the human lymphocyte culture and in bone marrow cells of Albino mice. Egypt J Med Hum Genet 15: 227-233. google scholar
  • Allegra M, De Cicco P, Ercolano G, Attanzio A, Busà R, Cirino G, Ianaro A (2018). Indicaxanthin from Opuntia Ficus Indica (L. Mill) impairs melanoma cell proliferation, invasiveness, and tumor progression. Phytomedicine 50: 19-24. google scholar
  • Ammar I, Ben Salem M, Harrabi B, Mzid M, Bardaa S, Sahnoun Z (2018). Anti-inflammatory activity and phenolic composition of prickly pear (Opuntia ficus-indica) flowers. Ind Crops Prod 112: 313-319. google scholar
  • Becer E, Kabadayı H, Meriçli F, Meriçli AH, Kıvançlı B, Vatansever S (2018). Apoptotic Effects of Opuntia ficus indica L. Seed Oils on Colon Adenocarcinoma Cell Lines. In MDPI 2: 1566. google scholar
  • Bishayee A, Sethi G (2016). Bioactive natural products in cancer prevention and therapy: Progress and promise. Semin Cancer Biol 40-41: 1-3. google scholar
  • Blois MS (1958). Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. google scholar
  • Boots AW, Haenen GRMM, Bast A (2008). Health effects of quercetin: From antioxidant to nutraceutical. Eur J Pharmacol 585: 325-337. google scholar
  • Bouaouine O, Bourven I, Khalil F, Baudu M (2018). Identification of functional groups of Opuntia ficus-indica involved in coagulation process after its active part extraction. Environ Sci Pollut Res (11): 11111-11119. google scholar
  • Butera D, Tesoriere L, Di Gaudio F, Bongiorno A, Allegra M, Pintaudi AM (2002). Antioxidant activities of sicilian prickly pear (Opuntia ficus indica) fruit extracts and reducing properties of its betalains: Betanin and indicaxanthin. J Agric Food Chem 50: 6895-6901. google scholar
  • Cai Y, Luo Q, Sun M, Corke H (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 74: 2157-2184. google scholar
  • Carroll J, Protani M, Walpole E, Martin JH (2012). Effect of obesity on toxicity in women treated with adjuvant chemotherapy for early-stage breast cancer: a systematic review. Breast Cancer Res Treat 136: 323-330. google scholar
  • Chahar M, Sharma N, Dobhal M, Joshi Y (2011). Flavonoids: A versatile source of anticancer drugs. Pharmacogn Rev 5: 1. google scholar
  • Demain AL, Vaishnav P (2011). Natural products for cancer chemotherapy. Microb Biotechnol 4: 687-699. google scholar
  • Dok-Go H, Lee KH, Kim HJ, Lee EH, Lee J, Song YS (2003). Neuroprotective effects of antioxidative flavonoids, quercetin, (+)-dihydroquercetin and quercetin 3-methyl ether, isolated from Opuntia ficus-indica var. saboten. Brain Res 965: 130-136. google scholar
  • El-Beltagi HS, Mohamed HI, Elmelegy AA, Eldesoky SE, Safwat, G (2019). Phytochemical Screening, Antimicrobial, Antioxidant, Anticancer Activities And Nutritional Values Of Cactus (Opuntia Ficus Indicia) Pulp And Peel. Fresen Environ Bull 28: 1545-1562. google scholar
  • Gulsoy-Toplan G, Goger F, Yildiz-Peko A, Gibbons S, Sariyar G, Mat A (2018). Chemical Constituents of the Different Parts of Colchicum micranthum and C. chalcedonicum and their Cytotoxic Activities. Nat Prod Commun 13: 535-538. google scholar
  • Güner A, Aslan S, Ekim T, Vural M, Babac MT, Yıldırım H. Turkey Plant List (Vascular Plants). İstanbul: Nezahat Gökyiğit Botanic Park Press; 2012. google scholar
  • Jiménez-Estrada M, Velázquez-Contreras C, Garibay-Escobar A, Sierras-Canchola D, Lapizco-Vázquez R, Ortiz-Sandoval C (2013). In vitro antioxidant and antiproliferative activities of plants of the ethnopharmacopeia from northwest of Mexico. BMC Complement Altern Med 13: 12. google scholar
  • Kabas O, Ozmerzi A, Akinci I (2006). Physical properties of cactus pear (Opuntia ficus india L.) grown wild in Turkey. J Food Eng 73: 198-202. google scholar
  • Kaur M, Kaur A, Sharma R (2012). Pharmacological actions of Opuntia ficus indica: A Review. J Appl Pharm 2: 15-18. google scholar
  • Khanapur M, Avadhanula RK, Setty OH (2014). In vitro antioxidant, antiproliferative, and phytochemical study in different extracts of Nyctanthes arbortristis flowers. Biomed Res Int 2014: 291271. google scholar
  • Lee CS, Ryan EJ, Doherty GA (2014). Gastro-intestinal toxicity of chemotherapeutics in colorectal cancer: the role of inflammation. World J Gastroenterol 20: 3751-3761. google scholar
  • Lee J-C, Kim H-R, Kim J, Jang Y-S (2002). Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. saboten. J Agric Food Chem 50: 6490-6496. google scholar
  • Lee WC, Mahmud R, Pillai S, Perumal S, Ismail S (2012). Antioxidant Activities of Essential Oil of Psidium Guajava L. Leaves. APCBEE Procedia 2: 86-91. google scholar
  • Livshits Z, Rao RB, Smith SW (2014). An Approach to Chemotherapy-Associated Toxicity. Emerg Med Clin North Am 32: 167-203. google scholar
  • Lu J, Papp L V., Fang J, Rodriguez-Nieto S, Zhivotovsky B, Holmgren A (2006). Inhibition of Mammalian Thioredoxin Reductase by Some Flavonoids: Implications for Myricetin and Quercetin Anticancer Activity. Cancer Res 66: 4410-4418. google scholar
  • Majumdar D, Jung K-H, Zhang H, Nannapaneni S, Wang X, Amin ARMR (2014). Luteolin Nanoparticle in Chemoprevention: In Vitro and In Vivo Anticancer Activity. Cancer Prev Res 7: 65-73. google scholar
  • Mann J (2002). Natural products in cancer chemotherapy: past, present and future. Nat Rev Cancer 2: 143-148. google scholar
  • Mena P, Tassotti M, Andreu L, Nuncio-Jáuregui N, Legua P, Del Rio D (2018). Phytochemical characterization of different prickly pear (Opuntia ficus-indica (L.) Mill.) cultivars and botanical parts: UHPLC-ESI-MS n metabolomics profiles and their chemometric analysis. Food Res Int 108: 301-318. google scholar
  • Okur ME, Ayla Ş, Çiçek Polat D, Günal MY, Yoltaş A, Biçeroğlu Ö (2018a). Novel insight into wound healing properties of methanol extract of Capparis ovata Desf. var. palaestina Zohary fruits. J Pharm Pharmacol 70: 1401-1413. google scholar
  • Okur ME, Karakaş N, Karadag AE, Yılmaz R, Demirci F (2019). In vitro cytotoxicity evaluation of Marrubium vulgare L. methanol extract. J Res Pharm 23: 711-718. google scholar
  • Okur ME, Polat DC, Ozbek H, Yilmaz S, Yoltas A, Arslan R (2018b). Evaluation of the antidiabetic property of capparis ovata desf. Var. Paleastina zoh. Extracts using in vivo and in vitro approaches. Endocrine, Metab Immune Disord - Drug Targets 18: 489-501. google scholar
  • Panche AN, Diwan AD, Chandra SR (2016). Flavonoids: an overview. J Nutr Sci 5:e47. google scholar
  • Rajesh E, Sankari L, Malathi L, Krupaa J (2015) Naturally occurring products in cancer therapy. J Pharm Bioallied Sci 7: 183. google scholar
  • Rauf A, Imran M, Khan IA, ur-Rehman M-, Gilani SA, Mehmood Z (2018). Anticancer potential of quercetin: A comprehensive review. Phyther Res 32: 2109-2130. google scholar
  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. google scholar
  • Rodenhizer D, Dean T, Xu B, Cojocari D, McGuigan AP (2018). A three-dimensional engineered heterogeneous tumor model for assessing cellular environment and response. Nat Protoc 13: 1917. google scholar
  • Sittampalam GS, Coussens NP, Brimacombe K, Grossman A, Arkin M, Auld D, Chung TDY (2004). Measurement of β-Arrestin Recruitment for GPCR Targets--Assay Guidance Manual. google scholar
  • Spanos GA, Wrolstad RE (1990). Influence of processing and storage on the phenolic composition of Thompson Seedless grape juice. J Agric Food Chem 8: 1565-1571. google scholar
  • Sreekanth D, Arunasree MK, Roy KR, Chandramohan Reddy T, Reddy G V, Reddanna P (2007). Betanin a betacyanin pigment purified from fruits of Opuntia ficus-indica induces apoptosis in human chronic myeloid leukemia Cell line-K562. Phytomedicine 14: 739-746. google scholar
  • Sun J, Chu Y-F, Wu X, Liu RH (2002). Antioxidant and antiproliferative activities of common fruits. J Agric Food Chem 50: 7449-7454. google scholar
  • Sun W, Tao Y, Yu D, Zhao T, Wu L, Yu W (2018). Myricetin exerts potent anticancer effects on human skin tumor cells. Trop J Pharm Res 17: 1067. google scholar
  • Üstündağ-Okur N, Gökçe EH, Bozbıyık Dİ, Eğrilmez S, Ertan G, Özer Ö (2015). Novel nanostructured lipid carrier-based inserts for controlled ocular drug delivery: evaluation of corneal bioavailability and treatment efficacy in bacterial keratitis. Expert Opin Drug Deliv 12: 1791-1807. google scholar
  • Zhang J, Ren L, Yang X, White M, Greenhaw J, Harris T, Shi Q (2018). Cytotoxicity of 34 FDA approved small-molecule kinase inhibitors in primary rat and human hepatocytes. Toxicol Lett 291: 138-148. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Okur, M., Karakaş, N., Karadağ, A., Uludağ, D., & Polat, D. (0001). Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. İstanbul Journal of Pharmacy, 49(3), 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035


AMA

Okur M, Karakaş N, Karadağ A, Uludağ D, Polat D. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. İstanbul Journal of Pharmacy. 0001;49(3):154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035


ABNT

Okur, M.; Karakaş, N.; Karadağ, A.; Uludağ, D.; Polat, D. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. İstanbul Journal of Pharmacy, [Publisher Location], v. 49, n. 3, p. 154-160, 0001.


Chicago: Author-Date Style

Okur, Mehmet Evren, and Nihal Karakaş and Ayşe Esra Karadağ and Damla Uludağ and Derya Çiçek Polat. 0001. “Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract.” İstanbul Journal of Pharmacy 49, no. 3: 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035


Chicago: Humanities Style

Okur, Mehmet Evren, and Nihal Karakaş and Ayşe Esra Karadağ and Damla Uludağ and Derya Çiçek Polat. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract.” İstanbul Journal of Pharmacy 49, no. 3 (Nov. 2024): 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035


Harvard: Australian Style

Okur, M & Karakaş, N & Karadağ, A & Uludağ, D & Polat, D 0001, 'Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract', İstanbul Journal of Pharmacy, vol. 49, no. 3, pp. 154-160, viewed 15 Nov. 2024, https://doi.org/10.26650/IstanbulJPharm.2019.19035


Harvard: Author-Date Style

Okur, M. and Karakaş, N. and Karadağ, A. and Uludağ, D. and Polat, D. (0001) ‘Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract’, İstanbul Journal of Pharmacy, 49(3), pp. 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035 (15 Nov. 2024).


MLA

Okur, Mehmet Evren, and Nihal Karakaş and Ayşe Esra Karadağ and Damla Uludağ and Derya Çiçek Polat. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract.” İstanbul Journal of Pharmacy, vol. 49, no. 3, 0001, pp. 154-160. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.19035


Vancouver

Okur M, Karakaş N, Karadağ A, Uludağ D, Polat D. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. İstanbul Journal of Pharmacy [Internet]. 15 Nov. 2024 [cited 15 Nov. 2024];49(3):154-160. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.19035 doi: 10.26650/IstanbulJPharm.2019.19035


ISNAD

Okur, Mehmet Evren - Karakaş, Nihal - Karadağ, Ayşe Esra - Uludağ, Damla - Polat, Derya Çiçek. Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract”. İstanbul Journal of Pharmacy 49/3 (Nov. 2024): 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035



TIMELINE


Accepted28.08.2019

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.