Original Research


DOI :10.26650/IstanbulJPharm.2019.0057   IUP :10.26650/IstanbulJPharm.2019.0057    Full Text (PDF)

Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies

Naveen Kumar KotlaShibnath KamilaShivani PatelJoel KothapallyAparna KongaraSatheesh Madhav

Background and Aims: The main aim of the study was to enhance the cognitive function of the brain by nootropic herbal formulations in animal models. Polyphyto herbal formulations were known to enhance the cognition and memory function by several pathways such as anti-oxidative, anti-inflammatory, and cell signaling pathways. In this study, six formulations were prepared by mixing specified plant parts and were coded as NHF1, NHF2, NHF3, NHF4, NHF5, and NHF6.

Methods: The potency of the formulations was assessed by In vivo (photo actometer, rod walking test, pole climbing test, and Ellman’s acetylcholinesterase test) studies.

Results: NHF1 and NHF5 exhibited greater activity than the standard drug donepezil in vivo (Ellman’s acetylcholinesterase test) analysis. NHF1 and NHF5 formulations containing plant parts were further investigated against several published literatures for the identification of chemical constituents and those chemical constituents were subjected to molecular docking and in silico ADME prediction studies to figure out the possible compounds responsible for the cholinesterase inhibition activity.

Conclusion: In conclusion, the computational studies also reveal that presence of chemical constituents such as sarsasapogenin (13.13 nM), racemosol (16.26 nM), and beta-sitosterol (30.47 nM) having binding energy (-10.75 kcal/mol), (-10.63 kcal/mol), (-10.25 kcal/mol), might be directly responsible for the nootropic activity.


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APA

Kotla, N.K., Kamila, S., Patel, S., Kothapally, J., Kongara, A., & Madhav, S. (2020). Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies. İstanbul Journal of Pharmacy, 50(2), 116-124. https://doi.org/10.26650/IstanbulJPharm.2019.0057


AMA

Kotla N K, Kamila S, Patel S, Kothapally J, Kongara A, Madhav S. Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies. İstanbul Journal of Pharmacy. 2020;50(2):116-124. https://doi.org/10.26650/IstanbulJPharm.2019.0057


ABNT

Kotla, N.K.; Kamila, S.; Patel, S.; Kothapally, J.; Kongara, A.; Madhav, S. Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies. İstanbul Journal of Pharmacy, [Publisher Location], v. 50, n. 2, p. 116-124, 2020.


Chicago: Author-Date Style

Kotla, Naveen Kumar, and Shibnath Kamila and Shivani Patel and Joel Kothapally and Aparna Kongara and Satheesh Madhav. 2020. “Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies.” İstanbul Journal of Pharmacy 50, no. 2: 116-124. https://doi.org/10.26650/IstanbulJPharm.2019.0057


Chicago: Humanities Style

Kotla, Naveen Kumar, and Shibnath Kamila and Shivani Patel and Joel Kothapally and Aparna Kongara and Satheesh Madhav. Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies.” İstanbul Journal of Pharmacy 50, no. 2 (May. 2024): 116-124. https://doi.org/10.26650/IstanbulJPharm.2019.0057


Harvard: Australian Style

Kotla, NK & Kamila, S & Patel, S & Kothapally, J & Kongara, A & Madhav, S 2020, 'Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies', İstanbul Journal of Pharmacy, vol. 50, no. 2, pp. 116-124, viewed 21 May. 2024, https://doi.org/10.26650/IstanbulJPharm.2019.0057


Harvard: Author-Date Style

Kotla, N.K. and Kamila, S. and Patel, S. and Kothapally, J. and Kongara, A. and Madhav, S. (2020) ‘Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies’, İstanbul Journal of Pharmacy, 50(2), pp. 116-124. https://doi.org/10.26650/IstanbulJPharm.2019.0057 (21 May. 2024).


MLA

Kotla, Naveen Kumar, and Shibnath Kamila and Shivani Patel and Joel Kothapally and Aparna Kongara and Satheesh Madhav. Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies.” İstanbul Journal of Pharmacy, vol. 50, no. 2, 2020, pp. 116-124. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2019.0057


Vancouver

Kotla NK, Kamila S, Patel S, Kothapally J, Kongara A, Madhav S. Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies. İstanbul Journal of Pharmacy [Internet]. 21 May. 2024 [cited 21 May. 2024];50(2):116-124. Available from: https://doi.org/10.26650/IstanbulJPharm.2019.0057 doi: 10.26650/IstanbulJPharm.2019.0057


ISNAD

Kotla, NaveenKumar - Kamila, Shibnath - Patel, Shivani - Kothapally, Joel - Kongara, Aparna - Madhav, Satheesh. Nootropic herbal formulations for the treatment of Alzheimer’s disease: In vivo pharmacological assay and molecular docking studies”. İstanbul Journal of Pharmacy 50/2 (May. 2024): 116-124. https://doi.org/10.26650/IstanbulJPharm.2019.0057



TIMELINE


Submitted30.09.2019
Accepted06.02.2020
Published Online27.08.2020

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