İstanbul Journal of Pharmacy

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Research Article


DOI :10.26650/IstanbulJPharm.2022.996448   IUP :10.26650/IstanbulJPharm.2022.996448    Full Text (PDF)

Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances

Cemre Özkanca, Sibel Döşler

Background and Aims: Recently, one of the biggest problems of the world is a bacterial antimicrobial resistance, that is developing against most of the existing antibiotics. In addition to conducting studies that continue to discover new antimicrobial agents for combating multidrug resistant bacteria, steps should be taken for the protection of existing antibiotics. With this in mind, many modern and classical strategies have been developed, and among them, using essential oils or extracts obtained from plants, which may be a practical and effective alternative. Methods: We used the experimental evolutionary microbiology method to determine the effects of herbal substances, such as cinnamaldehyde from cinnamon, epigallocatechin gallate from greentea, curcumin from turmeric, punicalagin from pomegranate, and clove oil from clove, on the prevention or delay of antimicrobial resistance. In this study, Staphylococcus aureus and Escherichia coli standard and clinical strains were gradually exposed to increasing sub-inhibitory concentrations of meropenem and ciprofloxacin with or without the presence of herbal substances. Results: Resistance was developed in the E. coli and S. aureus control groups which were exposed only to ciprofloxacin, but, when herbal substances were included to the test, there was no resistance development. When the control groups were exposed only to meropenem, there was only an increase in the minimum inhibitory concentrations (MIC), but they did not become resistant, and we observed similar MIC values when we added the herbal substances to the test. Conclusion: These results showed that herbal substances might contribute to lowering MIC values of antibiotics and may help prevent the development of resistance in the studied bacteria.

Keywords: Antibiotic resistance, Escherichia coli, Evolutionary microbiology, Herbal substance, Staphylococcus aureus

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APA

Özkanca, C., & Döşler, S. (2022). Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances. İstanbul Journal of Pharmacy, 52(2), 173-178. https://doi.org/10.26650/IstanbulJPharm.2022.996448


AMA

Özkanca C, Döşler S. Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances. İstanbul Journal of Pharmacy. 2022;52(2):173-178. https://doi.org/10.26650/IstanbulJPharm.2022.996448


ABNT

Özkanca, C.; Döşler, S. Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 2, p. 173-178, 2022.


Chicago: Author-Date Style

Özkanca, Cemre, and Sibel Döşler. 2022. “Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances.” İstanbul Journal of Pharmacy 52, no. 2: 173-178. https://doi.org/10.26650/IstanbulJPharm.2022.996448


Chicago: Humanities Style

Özkanca, Cemre, and Sibel Döşler. “Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances.” İstanbul Journal of Pharmacy 52, no. 2 (May. 2024): 173-178. https://doi.org/10.26650/IstanbulJPharm.2022.996448


Harvard: Australian Style

Özkanca, C & Döşler, S 2022, 'Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances', İstanbul Journal of Pharmacy, vol. 52, no. 2, pp. 173-178, viewed 18 May. 2024, https://doi.org/10.26650/IstanbulJPharm.2022.996448


Harvard: Author-Date Style

Özkanca, C. and Döşler, S. (2022) ‘Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances’, İstanbul Journal of Pharmacy, 52(2), pp. 173-178. https://doi.org/10.26650/IstanbulJPharm.2022.996448 (18 May. 2024).


MLA

Özkanca, Cemre, and Sibel Döşler. “Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances.” İstanbul Journal of Pharmacy, vol. 52, no. 2, 2022, pp. 173-178. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.996448


Vancouver

Özkanca C, Döşler S. Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances. İstanbul Journal of Pharmacy [Internet]. 18 May. 2024 [cited 18 May. 2024];52(2):173-178. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.996448 doi: 10.26650/IstanbulJPharm.2022.996448


ISNAD

Özkanca, Cemre - Döşler, Sibel. “Prevention of antibiotic resistance created by experimental evolutionary microbiology in Staphylococcus aureus and Escherichia coli with herbal substances”. İstanbul Journal of Pharmacy 52/2 (May. 2024): 173-178. https://doi.org/10.26650/IstanbulJPharm.2022.996448



TIMELINE


Submitted16.09.2021
Accepted27.03.2022
Published Online29.08.2022

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