Research Article


DOI :10.5152/IstanbulJPharm.2018.416835   IUP :10.5152/IstanbulJPharm.2018.416835    Full Text (PDF)

Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD

Ceren EmirNehir Ünver Somer

In this study, a reversed-phase, high pressure liquid chromatography (RP-HPLC) method was applied in the quantitative analysis of lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn. var. alpinus collected from Rize during the flowering and fruiting seasons. A simple method to extract lycorine from the plant specimens was performed, using pre-packed columns with diatomaceous earth (Extrelut®). The chromatographic separation was achieved employing an isocratic system with a mobile phase including trifluoroacetic acid-water-acetonitrile (0.01:95:5) measured at a flow rate of 1 mL/min using a diode array detector (DAD). The lycorine content in the bulbs during the flowering and fruiting seasons was detected as 0.01576 and 0.02351 %, respectively. Also, validation studies showed that the method was specific, accurate and precise.


PDF View

References

  • Berkov S, Codina C, Bastida J (2012). The genus Galanthus: A Source of bioactive compounds. In: Rao V (ed.) Phytochemicals- A Global Perspective of Their Role in Nutrition and Health. InTech, Rijeka, pp. 235-254. google scholar
  • Bishop M, Davis A, Grimshaw J (2006). Snowdrops. Griffin Press Publishing Ltd., Cheltenham: UK. google scholar
  • Doskočil I, Hošťálková A, Šafratová M, Benešová N, Havlík J, Havelek R, Kuneš J, Královec K, Chlebek J, Cahlíková L (2015). Cytotoxic activities of Amaryllidaceae alkaloids against gastrointestinal cancer cells. Phytochem Lett 13:394–8. google scholar
  • Emir A, Emir C, Bozkurt B, Onur MA, Somer NU, Kaya GI (2017). Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook. Brazilian J Pharm Sci 53(1):1–6. google scholar
  • Giordani RB, De Brum Vieira P, Weizenmann M, Rosemberg DB, Souza AP, Bonorino C, De Carli GA, Bogo MR, Zuanazzi JA, Tasca T (2011). Lycorine induces cell death in the amitochondriate parasite, Trichomonas vaginalis, via an alternative non-apoptotic death pathway. Phytochemistry 72(7):645–50. google scholar
  • Giordani RB, Junior COR, de Andrade JP, Bastida J, Zuanazzi JAS, Tasca T, de Almeida MV (2012). Lycorine derivatives against Trichomonas vaginalis. Chem Biol Drug Des 80(1):129–33. google scholar
  • Heinrich M, Teoh HL (2004). Galanthamine from snowdrop - The development of a modern drug against Alzheimer’s disease from local Caucasian knowledge. J Ethnopharmacol 92(2–3):147–62. google scholar
  • ICH Guidelines Q2(R1) (2005). Validation of Analytical procedures: Text and Methodology. Geneva. google scholar
  • Ilavenil S, Kaleeswaran B, Ravikumar S (2012). Protective effects of lycorine against carbon tetrachloride induced hepatotoxicity in Swiss albino mice. Fundam Clin Pharmacol 26:393–401. google scholar
  • Kaya G, Fillik A, Hışıl Y, Ünver N (2004). High pressure liquid chromatographic analysis of lycorine in four Galanthus species growing in Turkey. Turkish J Pharm Sci 1(2):105–14. google scholar
  • Kaya G, Çiçek D, Sarikaya B, Onur M, Somer NÜ (2014). Quantitative Determination of Lycorine and Galanthamine in Galanthus trojanus and G. cilicicus by HPLC-DAD. Nat Prod Commun 9(8):1157–1158. google scholar
  • Larsen MM, Andersen A, Davis AP, Lledó MD, Jäger AK, Rønsted N (2010). Using a phylogenetic approach to selection of target plants in drug discovery of acetylcholinesterase inhibiting alkaloids in Amaryllidaceae tribe Galantheae. Biochem Syst Ecol. 38:1026-1034. google scholar
  • Li SY, Chen C, Zhang HQ, Guo HY, Wang H, Wang L, Zhang X, Hua S, Yu J, Xiao P, Li R, Tan X (2005). Identification of natural compounds with antiviral activities against SARS-associated coronavirus. Antiviral Res 67(1):18–23. google scholar
  • Locarek M, Novakova J, Kloucek P, Host’alkova A, Kokoska L, Gabrlova L, Safratova M, Opletal L, Cahlíkova L (2015). Antifungal and antibacterial activity of extracts and alkaloids of selected Amaryllidaceae species. Nat Prod Commun. 10(9):1537–40. google scholar
  • Nair JJ, van Staden J (2014). Cytotoxicity studies of Lycorine alkaloids of the Amaryllidaceae. Nat Prod Commun 9(8):1193–210. google scholar
  • Nair JJ, van Staden J, Bastida J (2016). Apoptosis-Inducing Effects of Amaryllidaceae Alkaloids. Curr Med Chem 23:161–85. google scholar
  • Oluyemisi OO, Oriabure AE, Adekunle AJ, Soup K, Ramsay T, Shyyaula S (2015). Bioassay-guided isolation of Poliovirus-inhibiting constituents from Zephyranthes candida. Pharm Biol 53(6):882–7. google scholar
  • Saltan Çitoğlu G, Bahadir Acikara O, Sever Yilmaz B, Özbek H (2012). Evaluation of analgesic, anti-inflammatory and hepatoprotective effects of lycorine from Sternbergia fisheriana (Herbert) Rupr. Fitoterapia 83(1):81–7. google scholar
  • Sarikaya BB, Somer NU, Onur MA (2012). Quantitative Determinations on Galanthus rizehensis. Turkish J Pharm Sci 9(1):61–6. google scholar
  • Shen JW, Ruan Y, Ren W, Ma BJ, Wang XL, Zheng CF (2014). Lycorine: A potential broad-spectrum agent against crop pathogenic fungi. J Microbiol Biotechnol 24(3):354–8. google scholar
  • Şener B, Orhan I, Satayavivad J (2003). Antimalarial activity screening of some alkaloids and the plant extracts from Amaryllidaceae. Phytother Res 17(10):1220–3. google scholar
  • Unver N (2007). New skeletons and new concepts in Amaryllidaceae alkaloids. Phytochem Rev 6:125–35. google scholar
  • Unver Somer N, Cicek Polat D, Emir A, Onur M, Kaya G (2013). Quantitative analysis of lycorine in endemic Galanthus xvalentinei notho subsp. subplicatus by HPLC-DAD. Acta Chromatogr 25(2):331-338. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Emir, C., & Ünver Somer, N. (2018). Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD. İstanbul Journal of Pharmacy, 48(2), 32-37. https://doi.org/10.5152/IstanbulJPharm.2018.416835


AMA

Emir C, Ünver Somer N. Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD. İstanbul Journal of Pharmacy. 2018;48(2):32-37. https://doi.org/10.5152/IstanbulJPharm.2018.416835


ABNT

Emir, C.; Ünver Somer, N. Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD. İstanbul Journal of Pharmacy, [Publisher Location], v. 48, n. 2, p. 32-37, 2018.


Chicago: Author-Date Style

Emir, Ceren, and Nehir Ünver Somer. 2018. “Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD.” İstanbul Journal of Pharmacy 48, no. 2: 32-37. https://doi.org/10.5152/IstanbulJPharm.2018.416835


Chicago: Humanities Style

Emir, Ceren, and Nehir Ünver Somer. Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD.” İstanbul Journal of Pharmacy 48, no. 2 (Jun. 2025): 32-37. https://doi.org/10.5152/IstanbulJPharm.2018.416835


Harvard: Australian Style

Emir, C & Ünver Somer, N 2018, 'Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD', İstanbul Journal of Pharmacy, vol. 48, no. 2, pp. 32-37, viewed 4 Jun. 2025, https://doi.org/10.5152/IstanbulJPharm.2018.416835


Harvard: Author-Date Style

Emir, C. and Ünver Somer, N. (2018) ‘Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD’, İstanbul Journal of Pharmacy, 48(2), pp. 32-37. https://doi.org/10.5152/IstanbulJPharm.2018.416835 (4 Jun. 2025).


MLA

Emir, Ceren, and Nehir Ünver Somer. Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD.” İstanbul Journal of Pharmacy, vol. 48, no. 2, 2018, pp. 32-37. [Database Container], https://doi.org/10.5152/IstanbulJPharm.2018.416835


Vancouver

Emir C, Ünver Somer N. Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD. İstanbul Journal of Pharmacy [Internet]. 4 Jun. 2025 [cited 4 Jun. 2025];48(2):32-37. Available from: https://doi.org/10.5152/IstanbulJPharm.2018.416835 doi: 10.5152/IstanbulJPharm.2018.416835


ISNAD

Emir, Ceren - Ünver Somer, Nehir. Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD”. İstanbul Journal of Pharmacy 48/2 (Jun. 2025): 32-37. https://doi.org/10.5152/IstanbulJPharm.2018.416835



TIMELINE


Accepted19.07.2018

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE



Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.