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DOI :10.26650/IstanbulJPharm.2021.877929   IUP :10.26650/IstanbulJPharm.2021.877929    Full Text (PDF)

Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva

Aparna Ann Mathew, Raju Asirvatham, A Gowtham, Daisy Pa

Background and Aims: Toxic chemicals, pathogens and damaged cells trigger the inflammatory process and cause disease in the kidney, heart, brain, liver, lung and reproductive system. The present study evaluates the anti-inflammatory and immunomodulatory effect of Ayurvedic plants under the Murva category. Methods: Marsdenia tenacissima is an acceptable source of Murva. Sansevieria roxburghiana is considered as Murva in west Bengal. Methanol extracts of both plants were used to evaluate anti-inflammatory and immunomodulatory effects by certain in vitro methods. The in vitro anti-inflammatory effects of methanol extract Marsdenia tenacissima (MEMT) and methanol extract of Sansevieria roxburghiana (MESR) were assessed by heat and hypotonic induced haemolysis on the red blood cells of rats. Nitro blue tetrazolium (NBT) assay and inhibition of TNF-α release in DAL cell lines were conducted to assess the immunomodulatory potential of extracts. Results: MEMT, MESR and a combination of MEMT&MESR showed significant (p<0.001) inhibition of haemolysis on heat and hypotonic induced methods compared with the standard Diclofenac Sodium. In NBT reduction test, MEMT showed a more significant (p<0.001) result than MESR. Similarly, the inhibition of TNF-α release was also significantly enhanced by 400 µg/ mL of MEMT. Conclusion: The present research results revealed that Murva is a safe Ayurveda drug for the treatment of cancer and cardiac disease with the protection of systemic immunity and set free from an inflammatory condition.

Keywords: Murva; Marsdenia tenacissima, Sansevieria roxburghiana, in vitro anti-inflammatory, immunomodulatory, NBT assay

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References

  • Arya, V., & Guota, V. K. (2011). Review on some cardioprotective plants from Ayurveda. International Journal of Research in Ayurve-da and Pharmacy, 2(1), 80-83. google scholar
  • Bag, G. C., Devi, P. G., & Bhaigyabati, T. H. (2015). Assessment of total flavonoid content and antioxidant activity of methanolic rhizome extract of three Hedychium species of Manipur valley. International Journal of Pharmaceutical Sciences Review and Re-search, 30(1), 154-159. google scholar
  • Chen, L., Deng, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Li, Y., Wang, X., & Zhao, L. (2018). Inflammatory responses and inflammation-associated diseases in organs. Oncotarget, 9, 7204-7218. https:// doi.org/10.18632/oncotarget.23208 google scholar
  • Fan, W., Sun, L., Zhou, J. Q., Zhang, C., Qin, S., Tang, Y., Liu, Y., Lin, S. S., & Yuan, S. T. (2015). Marsdenia tenacissima extract induces G0/ G1 cell cycle arrest in human esophageal carcinoma cells by in-hibiting mitogen-activated protein kinase (MAPK) signaling path-way. Chinesejournal of natural medicines, 13(6), 428-437. https:// doi.org/10.1016/S1875-5364(15)30036-4 google scholar
  • Galhena, P. B., Samarakoon, S. R., Thabrew, M. I., Weerasinghe, G. A., Thammitiyagodage, M. G., Ratnasooriya, W. D., & Tennekoon, K. H. (2012). Anti-Inflammatory activity is a possible mechanism by which the polyherbal formulation comprised of Nigella sativa (Seeds), Hemidesmus indicus (Root), and Smilax glabra (Rhizome) mediates its antihepatocarcinogenic effects. Evidence-based com-plementary and alternative medicine: eCAM, 2012, 108626. https:// doi.org/10.1155/2012/108626 google scholar
  • Gogtay, N. J., Bhatt, H. A., Dalvi, S. S., & Kshirsagar, N. A. (2002). The use and safety of non-allopathic Indian medicines. Drug safety, 25(14), 1005-1019. https://doi.org/10.2165/00002018-200225140-0000 google scholar
  • Gul, R., Jan, S.U., Faridullah, S., Sherani, S., & Jahan, N. (2017). Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. The Scientific World Journal, 2017. https://doi.org/10.1155/2017/5873648 google scholar
  • Haldar, P. K., Kar, B., Bala, A., Bhattacharya, S., & Mazumder, U. K. (2010). Antitumor activity of Sansevieria roxburghiana rhizome against Ehrlich ascites carcinoma in mice. Pharmaceutical biology, 48(12), 1337-1343. https://doi.org/10.3109/13880201003792592 google scholar
  • Hatapakki. B. C., & Hukkeri, V. I. (2011). Antipyretic activity of root of Marsdenia tenacissima in rats. Journal of Natural Remedies, 11(2), 98-102. https://doi.org/10.18311/jnr/2011/433 google scholar
  • Hepsibah, A. H., & Jothi, G. J. (2017). A comparative study on the effect of solvents on the phytochemical profile and biological potential of Ormocarpum cochinchinense Auct. Non (Lour.) Mer-rill. International Journal of Pharmacy and Pharmaceutical Sciences, 9(1), 67-72. https://doi.org/10.22159/ijpps.2017v9i1.15126 Heroor, S., Beknal, A. K., & Mahurkar, N. (2013). Immunomodulato-ry activity of methanolic extracts of fruits and bark of Ficus glom-erata Roxb. in mice and on human neutrophils. Indian Journal of Pharmacology, 45(2), 130-135. google scholar
  • Huang, C. F., Lin, S. S., Liao, P. H., Young, S. C., & Yang, C. C. (2008). The immunopharmaceutical effects and mechanisms of herb medicine. Cellular & Molecular Immunology, 5(1), 23-31. https:// doi.org/10.1038/cmi.2008.3. google scholar
  • Huang, M. Y., Zhang, L. L., Ding, J., & Lu, J. J. (2018). Anticancer drug discovery from Chinese medicinal herbs. Chinese medicine, 13(1), 1-9. https://doi.org/10.1186/s13020-018-0192-y google scholar
  • Lokhande P D, Jagdale S C, Chabukswar A R (2006). Natural rem-edies for heart diseases. Indian Journal of Traditional Knowledge, 5(3), 420-427. google scholar
  • Mathew, A. A., Asirvatham, R., & Tomy, D. V. (2021). Cardioprotec-tive Effect of Marsdenia tenacissima and Sansevieria roxburghiana in Doxorubicin-induced Cardiotoxicity in Rats in vivo: The Role of Dresgenin and Lupeol. Turkish journal of pharmaceutical sciences, 18(3), 271-281. https://doi.org/10.4274/tjps.galenos.2020.27880 Munoz, M., Cedeno, R., Rodrıguez, J., Vander, Knaap, WP, Mialhe. E., & Bachere, E. (2000). Measurement of reactive oxygen interme-diate production in haemocytes of the penaeid shrimp, Penaeus vannamei. Aquaculture, 191, 89-107. google scholar
  • Obydulla, O. (2016). Sansevieria roxburghiana Schult. & Schult. F., Agavaceae: phytochemistry, traditional uses and its pharmaco-logical activities-a review. World scientific new, 59, 24-34. google scholar
  • Prabhakar, U., Brooks, D. P., Lipshlitz, D., & Esser, K. M. (1995). Inhi-bition of LPS- induced TNF alpha production in human mono-cytes by adenosine (A2) receptor selective agonists. International journal of immunopharmacology, 17(3), 221-224. https://doi. org/10.1016/0192-0561(94)00096-7 google scholar
  • Raju, A., Seeja, S. R., Deshami, S. S., Sinchu, Y., & Malu, P. (2015). Cell membrane stabilizing effect of ethanol and aqueous extract of Humboldtia vahliana Wight an in vitro study. Spatula DD, 5(3), 171-175. google scholar
  • Sakthivel, K. M., & Guruvayoorappan, C. (2013). Acacia ferruginea inhibits tumor progression by regulating inflammatory medi-ators-(TNF-a, iNOS, COX-2, IL-10, IL-6, IFN-y, IL-2, GM-CSF) and pro-angiogenic growth factor- VEGF. Asian Pacific journal of can-cer prevention: APJCP, 14(6), 3909-3919. https://doi.org/10.7314/ apjcp.2013.14.6.3909 google scholar
  • da Silva, A. P., Nascimento da Silva, L. C., Martins da Fonseca, C. S., de Araujo, J. M., Correia, M. T., Cavalcanti, M., & Lima, V. L. (2016). Antimicrobial Activity and Phytochemical Analysis of Organic Ex-tracts from Cleome spinosa Jaqc. Frontiers in microbiology, 7, 963. https://doi.org/10.3389/fmicb.2016.00963 google scholar
  • Tiwari, V., Singh, A., & Tiwari, A. (2018). Phytopharmacological overview on controversial drug: Murva. Traditional and Folk Herbal Medicine: Recent Researches, 2, 475-526. google scholar
  • Trease, G. E., & Evans, W. C. (1989). Pharmacognosy. 13th (ed). ELBS/Bailliere Tindall, London. Pp.345-346, 535-536, 772-773. google scholar
  • Ven Murthy, M. R., Ranjekar, P. K., Ramassamy, C., & Deshpande, M. (2010) Scientific basis for the use of Indian ayurvedic medicinal plants in the treatment of neurodegenerative disorders: ashwagandha. Central Nervous System Agents in Medici-nal Chemistry, 10(3), 238-246. google scholar
  • Yamada, Y., Webber, E. M., Kirillova, I., Peschon, J. J., & Fausto, N. (1998). Analysis of liver regeneration in mice lacking type 1 or type 2 tumor necrosis factor receptor: Requirement for type 1 but not type 2 receptor. Hepatology (Baltimore, Md.), 28(4), 959-970. https://doi.org/10.1002/hep.510280410 google scholar

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APA

Mathew, A.A., Asirvatham, R., Gowtham, A., & Pa, D. (2021). Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva. İstanbul Journal of Pharmacy, 51(3), 333-340. https://doi.org/10.26650/IstanbulJPharm.2021.877929


AMA

Mathew A A, Asirvatham R, Gowtham A, Pa D. Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva. İstanbul Journal of Pharmacy. 2021;51(3):333-340. https://doi.org/10.26650/IstanbulJPharm.2021.877929


ABNT

Mathew, A.A.; Asirvatham, R.; Gowtham, A.; Pa, D. Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva. İstanbul Journal of Pharmacy, [Publisher Location], v. 51, n. 3, p. 333-340, 2021.


Chicago: Author-Date Style

Mathew, Aparna Ann, and Raju Asirvatham and A Gowtham and Daisy Pa. 2021. “Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva.” İstanbul Journal of Pharmacy 51, no. 3: 333-340. https://doi.org/10.26650/IstanbulJPharm.2021.877929


Chicago: Humanities Style

Mathew, Aparna Ann, and Raju Asirvatham and A Gowtham and Daisy Pa. “Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva.” İstanbul Journal of Pharmacy 51, no. 3 (Jun. 2024): 333-340. https://doi.org/10.26650/IstanbulJPharm.2021.877929


Harvard: Australian Style

Mathew, AA & Asirvatham, R & Gowtham, A & Pa, D 2021, 'Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva', İstanbul Journal of Pharmacy, vol. 51, no. 3, pp. 333-340, viewed 27 Jun. 2024, https://doi.org/10.26650/IstanbulJPharm.2021.877929


Harvard: Author-Date Style

Mathew, A.A. and Asirvatham, R. and Gowtham, A. and Pa, D. (2021) ‘Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva’, İstanbul Journal of Pharmacy, 51(3), pp. 333-340. https://doi.org/10.26650/IstanbulJPharm.2021.877929 (27 Jun. 2024).


MLA

Mathew, Aparna Ann, and Raju Asirvatham and A Gowtham and Daisy Pa. “Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva.” İstanbul Journal of Pharmacy, vol. 51, no. 3, 2021, pp. 333-340. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2021.877929


Vancouver

Mathew AA, Asirvatham R, Gowtham A, Pa D. Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva. İstanbul Journal of Pharmacy [Internet]. 27 Jun. 2024 [cited 27 Jun. 2024];51(3):333-340. Available from: https://doi.org/10.26650/IstanbulJPharm.2021.877929 doi: 10.26650/IstanbulJPharm.2021.877929


ISNAD

Mathew, AparnaAnn - Asirvatham, Raju - Gowtham, A - Pa, Daisy. “Study of in-vitro anti-inflammatory and immunomodulatory effect of Ayurvedic plants – Murva”. İstanbul Journal of Pharmacy 51/3 (Jun. 2024): 333-340. https://doi.org/10.26650/IstanbulJPharm.2021.877929



TIMELINE


Submitted18.02.2021
Accepted24.06.2021
Published Online22.11.2021

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