İstanbul Journal of Pharmacy

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Research Article


DOI :10.26650/IstanbulJPharm.2021.1016208   IUP :10.26650/IstanbulJPharm.2021.1016208    Full Text (PDF)

Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera

Nurdan Yazıcı Bektaş, Burak Barut, Emel Mataracı Kara, Yeter Yeşil Cantürk

Background and Aims: This study aims to determine total phenolic, total flavonoid contents and in vitro biological activities of methanol (CEP-1), n-butanol (CEP-2), water (CEP-3), n-hexane (CEP-4) extracts obtained from Cephalaria procera. Methods: The total phenolic and flavonoid content analysis, in vitro DPPH radical scavenging activities, cholinesterase, and tyrosinase inhibitory properties of the extracts were evaluated using spectrophotometric assays. DNA-damage and DNA-damage protective effects of the extracts were examined using agarose gel electrophoresis method. Antimicrobial activities of the extracts were determined by microdilution method. Results: CEP-3 had the best total phenolic content (79.64±1.11 mg GAE/g dry weight), and CEP-1 had the highest total flavonoid content (15.33±0.27 mg QEE/g dry weight) among tested extracts. CEP-1 showed the highest radical scavenging activity with 83.21±3.20 µg/mL of IC50 value. CEP-3 exerted the highest AChE and BuChE inhibitory action with 134.63±4.49 µg/mL and 62.76±0.63 µg/mL of IC50 values, respectively. CEP-3 showed significant tyrosinase inhibitory action with 51.95±0.35 µg/mL IC50 value compared to kojic acid (58.26±0.25 µg/mL). CEP-1 and CEP-3 were tested, and the both extracts did not damage supercoiled DNA at studied concentrations. Incidentally, results indicated that CEP-1 and CEP-3 protected supercoiled DNA against Fenton’s reagents. CEP-4 exhibited the highest antimicrobial activity on C. tropicalis with the MIC value of 156.2 µg/mL. Conclusion: The results showed that crude and subextracts of C. procera exerted several moderate activities on tested systems. It suggested that the species might be a promising medicinal plant for the treatment or prevention of several diseases associated with skin damage and wounds.

Keywords: Antioxidant, antimicrobial, anticholinesterase, Cephalaria procera, DNA protective, tyrosinase

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APA

Yazıcı Bektaş, N., Barut, B., Mataracı Kara, E., & Yeşil Cantürk, Y. (2021). Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera. İstanbul Journal of Pharmacy, 51(3), 365-371. https://doi.org/10.26650/IstanbulJPharm.2021.1016208


AMA

Yazıcı Bektaş N, Barut B, Mataracı Kara E, Yeşil Cantürk Y. Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera. İstanbul Journal of Pharmacy. 2021;51(3):365-371. https://doi.org/10.26650/IstanbulJPharm.2021.1016208


ABNT

Yazıcı Bektaş, N.; Barut, B.; Mataracı Kara, E.; Yeşil Cantürk, Y. Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera. İstanbul Journal of Pharmacy, [Publisher Location], v. 51, n. 3, p. 365-371, 2021.


Chicago: Author-Date Style

Yazıcı Bektaş, Nurdan, and Burak Barut and Emel Mataracı Kara and Yeter Yeşil Cantürk. 2021. “Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera.” İstanbul Journal of Pharmacy 51, no. 3: 365-371. https://doi.org/10.26650/IstanbulJPharm.2021.1016208


Chicago: Humanities Style

Yazıcı Bektaş, Nurdan, and Burak Barut and Emel Mataracı Kara and Yeter Yeşil Cantürk. “Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera.” İstanbul Journal of Pharmacy 51, no. 3 (Jul. 2024): 365-371. https://doi.org/10.26650/IstanbulJPharm.2021.1016208


Harvard: Australian Style

Yazıcı Bektaş, N & Barut, B & Mataracı Kara, E & Yeşil Cantürk, Y 2021, 'Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera', İstanbul Journal of Pharmacy, vol. 51, no. 3, pp. 365-371, viewed 2 Jul. 2024, https://doi.org/10.26650/IstanbulJPharm.2021.1016208


Harvard: Author-Date Style

Yazıcı Bektaş, N. and Barut, B. and Mataracı Kara, E. and Yeşil Cantürk, Y. (2021) ‘Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera’, İstanbul Journal of Pharmacy, 51(3), pp. 365-371. https://doi.org/10.26650/IstanbulJPharm.2021.1016208 (2 Jul. 2024).


MLA

Yazıcı Bektaş, Nurdan, and Burak Barut and Emel Mataracı Kara and Yeter Yeşil Cantürk. “Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera.” İstanbul Journal of Pharmacy, vol. 51, no. 3, 2021, pp. 365-371. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2021.1016208


Vancouver

Yazıcı Bektaş N, Barut B, Mataracı Kara E, Yeşil Cantürk Y. Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera. İstanbul Journal of Pharmacy [Internet]. 2 Jul. 2024 [cited 2 Jul. 2024];51(3):365-371. Available from: https://doi.org/10.26650/IstanbulJPharm.2021.1016208 doi: 10.26650/IstanbulJPharm.2021.1016208


ISNAD

Yazıcı Bektaş, Nurdan - Barut, Burak - Mataracı Kara, Emel - Yeşil Cantürk, Yeter. “Total phenolic, total flavonoid contents, and in vitro biological activities of Cephalaria procera”. İstanbul Journal of Pharmacy 51/3 (Jul. 2024): 365-371. https://doi.org/10.26650/IstanbulJPharm.2021.1016208



TIMELINE


Submitted01.10.2021
Accepted01.11.2021
Published Online04.11.2021

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