Research Article


DOI :10.26650/JARHS2024-1399963   IUP :10.26650/JARHS2024-1399963    Full Text (PDF)

ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS

Nermin AkçalıSaliha Handan YıldızMüjgan Özdemir ErdoğanMustafa SolakMine Kanat Pektaş

Objective: This study evaluated chromosome polymorphisms (1qh+, 9qh+, inv9, 13ps+, 14ps+, 15ps+, 16qh+, 21 ps+, 22 ps+ and Yqh+) in a case group (n=1688) with two or more recurrent pregnancy losses (RPL) and a control group (n=80).

Materials and Methods: The control group was selected from 40 married couples who had no known hereditary disease, were not relatives, had healthy children, and had no history of miscarriage and/or stillbirth. Phytohemagglutinin-induced peripheral blood lymphocytes were cultured for 72 h. The Giemsa–Trypsin–Leischman (GTL) banding technique was applied to the obtained metaphase plates; thirty metaphase plates were examined at the 450-550 band level in each individual.

Results: A total of 488 individuals in the case group and 13 in the control group carried polymorphic chromosome variants.9qh+ chromosome polymorphisms were more prevalent in the case group than in the control group. Other variants were also increasingly observed in the case group.

Conclusion: Our findings reveal a potential relationship between RPL and chromosome polymorphisms. Karyotype analysis and appropriate genetic counseling increase the chance of having healthy children in individuals with RPL.

DOI :10.26650/JARHS2024-1399963   IUP :10.26650/JARHS2024-1399963    Full Text (PDF)

TEKRARLAYAN GEBELİK KAYBI OLAN ÇİFTLERDE POLİMORFİK KROMOZOM VARYANTLARININ ANALİZİ

Nermin AkçalıSaliha Handan YıldızMüjgan Özdemir ErdoğanMustafa SolakMine Kanat Pektaş

Amaç: Bu çalışmada, iki veya daha fazla tekrarlayan gebelik kaybı (TGK) olan vaka grubu (n=1688) ve kontrol grubu (n=80) kromozom polimorfizmleri (1qh+, 9qh+, inv9, 13ps+, 14ps+, 15ps+, 16qh+, 21 ps+, 22 ps+ ve Yqh+) açısından değerlendirildi.

Gereç ve Yöntem: Kontrol grubu bilinen bir kalıtsal hastalığı olmayan, akraba olmayan, sağlıklı çocuğu olan, düşük ve/veya ölü doğum öyküsü olmayan 40 evli çiftten seçildi. Fitohemaglutinin ile indüklenmiş periferik kan lenfositlerinin 72 saatlik kültürü yapıldı. Elde edilen metafaz plaklarına Giemsa-Trypsin-Leischman (GTL) bantlama tekniği uygulandı. Her birey için yapılan kromozom analizinde 450-550 bant seviyesinde 30 metafaz plağı incelendi.

Bulgular: Polimorfik kromozom varyantı taşıyan bireylerin sayısı vaka grubunda 488 ve kontrol grubunda ise 13 olarak belirlendi. 9qh+ kromozom polimorfizminin kontrole göre vaka grubunda daha yüksek oranda olduğu belirlendi. Bununla birlikte, diğer bazı varyantların da vaka grubunda artış eğilimi gösterdiği gözlendi.

Sonuç: Bulgularımız TGK ile kromozom polimorfizmleri arasında bir ilişki olabileceğini kanıtladı. TGK olan bireylerde karyotip analizi ve uygun genetik danışma sağlıklı çocuk sahibi olma şansını artırmaktadır


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APA

Akçalı, N., Yıldız, S.H., Özdemir Erdoğan, M., Solak, M., & Kanat Pektaş, M. (2019). ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS. Journal of Advanced Research in Health Sciences, 0(0), -. https://doi.org/10.26650/JARHS2024-1399963


AMA

Akçalı N, Yıldız S H, Özdemir Erdoğan M, Solak M, Kanat Pektaş M. ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS. Journal of Advanced Research in Health Sciences. 2019;0(0):-. https://doi.org/10.26650/JARHS2024-1399963


ABNT

Akçalı, N.; Yıldız, S.H.; Özdemir Erdoğan, M.; Solak, M.; Kanat Pektaş, M. ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 0, n. 0, p. -, 2019.


Chicago: Author-Date Style

Akçalı, Nermin, and Saliha Handan Yıldız and Müjgan Özdemir Erdoğan and Mustafa Solak and Mine Kanat Pektaş. 2019. “ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS.” Journal of Advanced Research in Health Sciences 0, no. 0: -. https://doi.org/10.26650/JARHS2024-1399963


Chicago: Humanities Style

Akçalı, Nermin, and Saliha Handan Yıldız and Müjgan Özdemir Erdoğan and Mustafa Solak and Mine Kanat Pektaş. ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS.” Journal of Advanced Research in Health Sciences 0, no. 0 (May. 2024): -. https://doi.org/10.26650/JARHS2024-1399963


Harvard: Australian Style

Akçalı, N & Yıldız, SH & Özdemir Erdoğan, M & Solak, M & Kanat Pektaş, M 2019, 'ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS', Journal of Advanced Research in Health Sciences, vol. 0, no. 0, pp. -, viewed 4 May. 2024, https://doi.org/10.26650/JARHS2024-1399963


Harvard: Author-Date Style

Akçalı, N. and Yıldız, S.H. and Özdemir Erdoğan, M. and Solak, M. and Kanat Pektaş, M. (2019) ‘ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS’, Journal of Advanced Research in Health Sciences, 0(0), pp. -. https://doi.org/10.26650/JARHS2024-1399963 (4 May. 2024).


MLA

Akçalı, Nermin, and Saliha Handan Yıldız and Müjgan Özdemir Erdoğan and Mustafa Solak and Mine Kanat Pektaş. ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS.” Journal of Advanced Research in Health Sciences, vol. 0, no. 0, 2019, pp. -. [Database Container], https://doi.org/10.26650/JARHS2024-1399963


Vancouver

Akçalı N, Yıldız SH, Özdemir Erdoğan M, Solak M, Kanat Pektaş M. ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS. Journal of Advanced Research in Health Sciences [Internet]. 4 May. 2024 [cited 4 May. 2024];0(0):-. Available from: https://doi.org/10.26650/JARHS2024-1399963 doi: 10.26650/JARHS2024-1399963


ISNAD

Akçalı, Nermin - Yıldız, SalihaHandan - Özdemir Erdoğan, Müjgan - Solak, Mustafa - Kanat Pektaş, Mine. ANALYSIS OF POLYMORPHIC CHROMOSOME VARIANTS IN COUPLES WITH RECURRENT PREGNANCY LOSS”. Journal of Advanced Research in Health Sciences 0/0 (May. 2024): -. https://doi.org/10.26650/JARHS2024-1399963



TIMELINE


Submitted04.12.2023
Accepted12.02.2024
Published Online16.04.2024

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