Journal of Advanced Research in Health Sciences

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Research Article


DOI :10.26650/JARHS2025-1671717   IUP :10.26650/JARHS2025-1671717    Full Text (PDF)

The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis

Büşra Karaçam, Imran Khan, Elif Burce Elbasan, Sadaf Mahfooz, Kerime Akdur, Mustafa Aziz Hatiboğlu

Objective: The treatment modality for brain metastases (BM) is surgical resection or radiotherapy. Gamma Knife radiosurgery (GKR) treatment prevents toxicity to healthy brain tissue; therefore, this modality is preferred for treating selected patients with BM. Our aim in this study was to investigate the role of cfDNA levels, exosome levels, and miR-208a expressions as biomarkers for diag nosis and predicting the outcome in patients with BM treated with GKR.

Material and Methods: We included nine patients with brain metastasis who received GKR in this study. Samples were taken from patients at different time intervals (before GKR, 1 month after GKR, and at recurrence). Eight age- and gender-matched eight controls were used as healthy individuals. cfDNA, exosome, serum miRNA, and serum exosomal miRNA were isolated from the patient and control groups.

Results: The levels of cfDNA and exosomes were higher in BM patients than in the healthy group. Exosome and cfDNA levels decreased after GKR and increased at recurrence. The expression of serum miR-208a and serum exosomal miR-208a was increased after GKR. The expression of serum miR-208a was significantly higher in patients with lung cancer than in healthy controls and non-lung cancer patients. The expression of serum exosomal miR-208a was found to be significantly higher in patients with lung cancer than in healthy controls.

Conclusion: Our results showed that cfDNA and exosome levels might be used as diagnostic markers for BM patients.

Keywords: Brain metastasis, cfDNA, exosome, miRNA
DOI :10.26650/JARHS2025-1671717   IUP :10.26650/JARHS2025-1671717    Full Text (PDF)

Beyin Metastazı için Gamma Knife Radyocerrahisi ile Tedavi Edilen Hastalarda Likit Biyopsinin Rolü

Büşra Karaçam, Imran Khan, Elif Burce Elbasan, Sadaf Mahfooz, Kerime Akdur, Mustafa Aziz Hatiboğlu

Amaç: Beyin metastazlarının (BM) tedavi yöntemi cerrahi rezek siyon ve/veya radyoterapidir. Gamma Knife radyocerrahisi (GKR) tedavisi, sağlıklı beyin dokusuna toksisiteyi önler. Bu nedenle, BM'li seçilmiş hastaların tedavisinde tercih edilen bir modalitedir. Bu çalışmada, GKR ile tedavi edilen BM hastalarında tanı koyma ve tedavi sonucunu öngörmede biyobelirteç olarak cfDNA seviyeleri, eksozom seviyeleri ve miR-208a ekspresyonunun rolünün araştırıl ması amaçlandı.

Gereç ve Yöntemler: Bu çalışmaya GKR uygulanan beyin metastazlı dokuz hasta dahil edildi. Hastalardan farklı zaman aralıklarında (GKR öncesi, GKR sonrası 1. ay ve nüks döneminde) örnekler alındı. Yaş ve cinsiyet açısından eşleştirilmiş sekiz sağlıklı birey kontrol grubu olarak kullanıldı. Hasta ve kontrol grubundan cfDNA, ekso zom, serum miRNA ve serum ekzozomal miRNA izole edildi.

Bulgular: BM hastalarında cfDNA ve eksozom düzeyleri sağlıklı gruba kıyasla daha yüksek bulundu. GKR sonrası cfDNA ve ekso zom düzeyleri azaldığı, ancak nüks döneminde tekrar arttığı tespit edildi. Serum miR-208a ve serum ekzozomal miR-208a ekspresy onu GKR sonrası artmış bulundu. Serum miR-208a ekspresyonu, akciğer kanseri hastalarında sağlıklı kontrollere ve akciğer kanseri olmayan hastalara kıyasla anlamlı derecede daha yüksek olduğu saptandı. Serum ekzozomal miR-208a ekspresyonu ise akciğer kanseri hastalarında sağlıklı kontrollere kıyasla anlamlı derecede yüksek bulundu.

Sonuç: Bulgularımız, cfDNA ve eksozom düzeylerinin BM hastaları için tanısal belirteç olarak kullanılabileceğini göstermektedir.

Keywords: Beyin metastazı, cfDNA, eksozom, miRNA

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APA

Karaçam, B., Khan, I., Elbasan, E.B., Mahfooz, S., Akdur, K., & Hatiboğlu, M.A. (2025). The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis. Journal of Advanced Research in Health Sciences, 8(2), 106-113. https://doi.org/10.26650/JARHS2025-1671717


AMA

Karaçam B, Khan I, Elbasan E B, Mahfooz S, Akdur K, Hatiboğlu M A. The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis. Journal of Advanced Research in Health Sciences. 2025;8(2):106-113. https://doi.org/10.26650/JARHS2025-1671717


ABNT

Karaçam, B.; Khan, I.; Elbasan, E.B.; Mahfooz, S.; Akdur, K.; Hatiboğlu, M.A. The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 8, n. 2, p. 106-113, 2025.


Chicago: Author-Date Style

Karaçam, Büşra, and Imran Khan and Elif Burce Elbasan and Sadaf Mahfooz and Kerime Akdur and Mustafa Aziz Hatiboğlu. 2025. “The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis.” Journal of Advanced Research in Health Sciences 8, no. 2: 106-113. https://doi.org/10.26650/JARHS2025-1671717


Chicago: Humanities Style

Karaçam, Büşra, and Imran Khan and Elif Burce Elbasan and Sadaf Mahfooz and Kerime Akdur and Mustafa Aziz Hatiboğlu. “The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis.” Journal of Advanced Research in Health Sciences 8, no. 2 (Sep. 2025): 106-113. https://doi.org/10.26650/JARHS2025-1671717


Harvard: Australian Style

Karaçam, B & Khan, I & Elbasan, EB & Mahfooz, S & Akdur, K & Hatiboğlu, MA 2025, 'The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis', Journal of Advanced Research in Health Sciences, vol. 8, no. 2, pp. 106-113, viewed 25 Sep. 2025, https://doi.org/10.26650/JARHS2025-1671717


Harvard: Author-Date Style

Karaçam, B. and Khan, I. and Elbasan, E.B. and Mahfooz, S. and Akdur, K. and Hatiboğlu, M.A. (2025) ‘The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis’, Journal of Advanced Research in Health Sciences, 8(2), pp. 106-113. https://doi.org/10.26650/JARHS2025-1671717 (25 Sep. 2025).


MLA

Karaçam, Büşra, and Imran Khan and Elif Burce Elbasan and Sadaf Mahfooz and Kerime Akdur and Mustafa Aziz Hatiboğlu. “The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis.” Journal of Advanced Research in Health Sciences, vol. 8, no. 2, 2025, pp. 106-113. [Database Container], https://doi.org/10.26650/JARHS2025-1671717


Vancouver

Karaçam B, Khan I, Elbasan EB, Mahfooz S, Akdur K, Hatiboğlu MA. The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis. Journal of Advanced Research in Health Sciences [Internet]. 25 Sep. 2025 [cited 25 Sep. 2025];8(2):106-113. Available from: https://doi.org/10.26650/JARHS2025-1671717 doi: 10.26650/JARHS2025-1671717


ISNAD

Karaçam, Büşra - Khan, Imran - Elbasan, ElifBurce - Mahfooz, Sadaf - Akdur, Kerime - Hatiboğlu, MustafaAziz. “The Role of Liquid Biopsy in Patients Treated with Gamma Knife Radiosurgery for Brain Metastasis”. Journal of Advanced Research in Health Sciences 8/2 (Sep. 2025): 106-113. https://doi.org/10.26650/JARHS2025-1671717



TIMELINE


Submitted14.04.2025
Accepted14.04.2025
Published Online19.06.2025

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