Journal of Advanced Research in Health Sciences

SUBMITINFORMATION
EnglishTürkçe

Research Article


DOI :10.26650/JARHS2019-652926   IUP :10.26650/JARHS2019-652926    Full Text (PDF)

Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy

Çiğdem Kekik Çınar, Sonay Temurhan, Sebahat Usta Akgül, Filiz Aydın

Spondyloarthropathy (SpA) is a group of multi-systemic diseases, whose pathogenesis is not known, characterized by spinal inflammation, peripheral arthritis, and with a lower frequency by extra-articular involvement. Brevverton and Schlosstein introduced the relationship between HLA-B27 and the disease. Along with HLA-B27, the relationship of the disease with other HLA molecules was also shown in studies. Taking this information as a starting point and knowing that the disease is related to ethnic differences, we aimed to investigate the role of the HLA-A and –B alleles in Turkish patients with SpA. Typing of the patients (n=784) was performed by the complement-dependent lymphotoxicity method. The HLA-A and –B tissue groups of the control group (n: 1060) were determined by using serological or molecular methods. The frequency of HLA-B27 in patients was determined as 27%. When B27-negative patients were compared with B27-negative controls, HLA-A29 was found significantly higher in the patients (p: 0.0003, pc: 0.004). Although HLA-B60 was found significantly higher in the patients (p: 0.02), a statistical significance could not be obtained after performing the Bonferroni correction method (pc>0.05). When B27positive patients and controls were compared, HLA-A3 (p:0.0005, pc:0.008), HLA-B35 (P<0.0001, pc<0.003), HLA-B51 (P<0.0001, pc<0.003), and HLA-B52 (P<0.0001, pc:0.03) were found significantly higher in the control group, while HLA-B27 allele is related with the development of the disease. It has been shown in other studies that other HLA molecules together with ethnic differences may have an effect in liability to and protectiveness from the disease. 

Keywords: HLA, Spondyloarthropaty, HLA-B27
DOI :10.26650/JARHS2019-652926   IUP :10.26650/JARHS2019-652926    Full Text (PDF)

Spondiloartropatili Hastalarda HLA Sınıf I Allel Sıklığı

Çiğdem Kekik Çınar, Sonay Temurhan, Sebahat Usta Akgül, Filiz Aydın

Spondiloartropati (SpA), spinal inflamasyon ve periferal artrit ile daha az oranda da eklem dışı tutulumla karakterize, patogenezi henüz tam olarak bilinmeyen multisistemik bir grup hastalıktır. Genetik faktörlerin hastalığın gelişiminde önemli rolü vardır. 1973 yılında Brevverton ve Schlosstein, HLA-B27 ile hastalık ilişkisini ortaya çıkarmıştır. HLA-B27 ile birlikte diğer HLA moleküllerinin (DR1, DR4, DR8, DR15, A24, B39 ve B60)hastalıkla ilişkisi yapılan çalışmalarda gösterilmiştir. Bu bilgilerden yola çıkarak ve etnik farklılığın da hastalıkla ilişkili olduğunu düşünerek SpA’lı Türk hastalarda HLA-A ve –B allellerinin rolünü araştırmayı amaçladık. Hastalardan (n=784) heparinli kan örneği alındı. Lenfosit izolasyonunu takiben komplemana bağımlı lenfositotoksisite yöntemi ile ticari kitler (Biotest- HLA-ABC tipleme plağı, 144X2, USA) kullanılarak tipleme yapıldı. Kontrol grubunun (n:1060) HLA-A ve –B doku grupları, serolojik veya moleküler yöntemler kullanılarak tespit edildi. Hastalarda HLA-B27 sıklığı %27 olarak saptandı. B27 negatif hastalar ile B27 negatif kontroller karşılaştırıldığında, hastalarda HLA-A29 anlamlı olarak yüksek bulundu (p:0.0003, pc:0.004, OR:2.6, CI:1.5-4.4). HLA-B60 hastalarda anlamlı olarak yüksek (p:0.02, OR:0.5, CI:0.2-0.9) bulunmasına rağmen Bonferroni doğrulama testi sonrası istatistiksel anlamlılık elde edilemedi (pc>0.05). B27 pozitif hastalarla kontrolleri karşılaştırdığımızda HLA-A3 (p:0.0005, pc:0.008, OR:0.4, CI:0.3-0.7), HLA-B35 (p<0.0001, pc<0.003, OR:0.3, CI:0.2-0.4), HLA-B51 (P<0.0001, pc<0.003, OR:0.3, CI:0.2-0.6) ve HLA-B52 (p:0.001, pc:0.03, OR:0.04, CI:0.002-0.7) kontrol grubunda, HLA-B27 (p<0.0001, pc<0.003, OR:52, CI:36.2-74.7) ise hasta grubunda anlamlı olarak yüksek bulundu. Patogenezi tam olarak bilinmeyen SpA’ların gelişiminde genetik faktörlerin rolü büyüktür. HLA-B27 allelinin hastalığın gelişimi ile ilişkili olduğu bilinmektedir. Etnik farklılıklarla birlikte diğer HLA moleküllerinin de hastalığa karşı yatkınlık ve koruyuculukta etkili olabileceği yapılan çalışmalarla gösterilmiştir. 

Keywords: HLA, Spondiloartropati, HLA-B27

PDF View

References

  • 1. Kahn MA. HLA-B27 subtypes in world populations. Curr Opin Rheumatol 1995;7:263. google scholar
  • 2. Chopra A, Raghunath D, Singh A. Spectrum of seronegative arthropathies (SSA) with special reference to HLA profiles. J Assoc Phys India 1990;38:351-5. google scholar
  • 3. Lau CS, Burgos-Vargas R, Louthrenoo W, Mok MY, Wordsworth P,Zheng QY. Features of spondyloarthropathies around the world. Rheum Dis Clin North Am 1998;24:753-70. google scholar
  • 4. Lopez de Castro JA. HLA-B27 and the pathogenesis of spondyloarthrapathies. Immunol Lett 2006;15:27-33. google scholar
  • 5. Reveille JD, Arnett FC. Spondyloarthritis: update on pathogenesis and management. Am J Med 2005;118:592-603. google scholar
  • 6. Brewerton DA, Caffrey M, Hart FD et al. Ankylosing spondylitis and HLA-B27. Lancet 1973;1:904-7. google scholar
  • 7. Schlostein L, Terasaki PI, Bluestone R, Pearson CM. High association of an HLA antigen, BW27, with ankylosing spondylitis. N Engl J Med 1973;288:704-6. google scholar
  • 8. Gonzales-Roces S, Alvarez MV, Gonzales S et al. HLA-B27 polymorphism and worldwide susceptibility to ankylosing spondylitis. Tissue Antigens 1997;49:116-2. google scholar
  • 9. Nasution AR, Mardjuadi A, Kunmartini S, Suryadhana NG et al. HLA-B27 subtypes positively and negatively associated with spondyloarthropathy. J Rheumatol 1997;24:1111-4. google scholar
  • 10. Oguz FS, Ocal L, Diler AS, Ozkul H, Asicioglu F, Kasapoglu E, Bozkurt G, Konice M, Carin M. HLA B-27 subtypes in Turkish patients with spondyloarthropathy and healthy controls. Dis Markers. 2004;20(6):309-12. google scholar
  • 11. Abualrous ET, Fritzsche S, Hein Z, Al-Balushi MS, Reinink P, Boyle LH, Wellbrock U, Antoniou AN, Springer S. F pocket flexibility influences the tapasin dependence of two differentially disease-associated MHC Class I proteins. Eur J Immunol. 2015 Jan 23. doi: 10.1002/eji.201445307. google scholar
  • 12. Gunal EK, Sarvan FO, Kamali S, Gul A, Inanc M, Carin M, Konice M, Aral O, Ocal L. Low frequency of HLA-B27 in ankylosing spondylitis patients from Turkey. Joint Bone Spine. 2008 May;75(3):299-302. google scholar
  • 13. Toussirot E, Wendling D. Immunogenetic of ankylosing spondylitis. Rev Med Interne 2006;27:762-71. google scholar
  • 14. Brown MA, Kennedy GL, Darke C, Gibson K, Pile KD, Shatford JL, et al. The effect of HLA-DR genes on susceptibility to and severity of ankylosing spondylitis. Arthritis Rheum 1998;41:460–5. google scholar
  • 15. Miehle W, Schattenkirchner M, Albert D, Bunge M. HLA-DR4 in ankylosing spondylitis with different patterns of joint involvement. Ann Rheum Dis 1985;44:39–44. google scholar
  • 16. Robinson WP, Van Der Linden SM, Khan MA, Rentsch HU, Cats A, Russell A, et al. HLA-Bw60 increases susceptibility to ankylosing spondylitis in HLA-B27 + patients. Arthritis Rheum 1989;32:1135–41. google scholar
  • 17. De Juan MD, Reta A, Cancio J, Belzunegui J, Cuadrado E. HLA-A*9, a probable secondary susceptibility marker to ankylosing spondylitis in Basque patients. Tissue Antigens 1999;53:161–6. google scholar
  • 18. Islam SM, Numaga J, Fujino Y, Masuda K, Ohda H, Hirata R, et al. HLA-DR8 and acute anterior uveitis in ankylosing spondylitis. Arthritis Rheum 1995;38:547–50. google scholar
  • 19. Vargas-Alarcón G, García A, Bahena S, Melín-Aldana H, Andrade F, Ibañez-de-Kasep G, et al. HLA-B and complotypes in Mexican patients with seronegative spondyloarthropathies. Ann Rheum Dis 1994;53:755–8. google scholar
  • 20. Maksymowych WP, Gorodezky C, Olivo A, Alaez C, Wong C, Burgos-Vargas R, et al. HLA-DRB1*08 influences the development of disease in Mexican mestizo with spondyloarthropathy. J Rheumatol 1997;24:904–7. google scholar
  • 21. Parasannanavar DJ, Rajadhyaksha A, Ghosh K. Role of HLA-B Alleles and Clinical Presentation of B27 Negative Spondyloarthritis Patients from Mumbai, Western India. Autoimmune Dis. 2014;2014:327315. doi: 10.1155/2014/327315. google scholar
  • 22. Koehler L, Kuipers JG, Zeidler H. Managing seronegative spondyloarthritis. Rheumatology 2000;39:360-8. google scholar
  • 23. Prete M, Guerriero S, Dammacco R, Fatone MC, Vacca A, Dammacco F, Racanelli V. Autoimmune uveitis: a retrospective analysis of 104 patients from a tertiary reference center. J Ophthalmic Inflamm Infect. 2014 Jul 24;4:17. google scholar
  • 24. Radouane A, Oudghiri M, Chakib A, Naya A, Belhouari A, El Malki A, Bennani S. HLA-B*27 allele associated to Behc¸ et’s disease and to anterior uveitis in Moroccan patients. Ann Biol Clin (Paris). 2011 JulAug;69(4):419-24. google scholar
  • 25. Gustincich S, Manfiolett G, Del Sal G, Schneider C, Carninci P. A fast method for high-quality genomic DNA extraction from whole human blood. Bio Techniques 1991;11:298-302. google scholar
  • 26. Olerup O, Zetterquist H. HLA-DRB1*01 subtyping by allele-specific PCR amplification: A sensitive, specific and rapid technique. Tissue Antigens 1991;37:197204. google scholar
  • 27. Terasaki PI, McClelland JD. Microdroplet assay of human serum cytotoxins. Nature 1964:204;998-1000. google scholar
  • 28. Şendur ÖF, Aydeniz A. Spondiloartropatilerin temel özellikleri ve ayırıcı tanı ve tedavisinin genel kriterleri. ADÜ Tıp Fakültesi Dergisi 2001:2(2);31-5. google scholar
  • 29. Vargas-Alarcón G, Londono JD, Hernandez-Pacheco G, Pacheco-Tena C, Castillo E, Cardiel MH, Granados J, Burgos-Vargas R. Effect of HLA-B and HLA-DR genes on susceptibility to and severity of spondyloarthropathies in Mexican patients. Ann Rheum Dis 2002:61;714-7. google scholar
  • 30. Madhavan R, Parthiban M, Panchapakesa Rajendran C, Chandrasekaran AN, Zake L, Sanjeevi B. HLA Class I and Class II association with Ankylosing spondylitis in a Southern Indian population. Ann NY Acad Sci 2002:958;403-7. google scholar
  • 31. Arnais-Villena A, Carin M, Bendikuze N, GomezCasado E, Moscosa J, Oguz FS, Sarper Diler A, De Pacho A, Allende J, Guillen J, Martinez Laso J. HLA alleles and haplotypes in Turkish population: relatedness to Kurds, Armanians and other Mediterraneans. Tissue Antigens 2001:57(4);308-17. google scholar
  • 32. Karahan GE, Seyhun Y, Oguz SF, Kekik C, Onal AE, Yazici H, Turkmen A, Aydin AE, Sever MS, Eldegez U, Carin MN. Impact of HLA on the underlying primary diseases in Turkish patients with end-stage renal disease. Renal Failure 2009:31;44-49. google scholar
  • 33. Wei JCC, Tsai WC, Lin HS, Tsai CY, Chou CT. HLA-B60 and B61 are strongly associated with ankylosing spondylitis in HLA-B27 negative Taiwan Chinese patients. Rheumatalogy 2004:43;839-42. google scholar
  • 34. Mahfoudh N, Siala M, Rihl M, Kammoun A, Frikha F, Fourati H, Younes M, Gdoura R, Gaddour L, Hakim F, Bahloul Z, Baklouti S, Bargaoui N, Sellami S , Hammami A, Makni H. Association and frequency of HLA-A, B and HLA-DR genes in south Tunisian patients with spondyloarthritis (SpA).Clin Rheumatol 2011: DOI 10.1007/s10067-011-1705-6 google scholar
  • 35. Pimentel-Santos FM, Matos M, Ligeiro D, Moura AF, Ribeiro C, Costa J, Santos H, Barcelos A, Pinto P, Cruz M, Sousa E, Santos RA, Fonseca JE, Trindade H, Guedes-Pinto H, Branco JC, CORPOREA Study Group. HLA alleles and HLA-B27 haplotypes associated with susceptibility and severity of ankylosing spondylitis in a Portuguese population. Tissue Antigens, 2013:82;374– 379. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Kekik Çınar, Ç., Temurhan, S., Usta Akgül, S., & Aydın, F. (2019). Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy. Journal of Advanced Research in Health Sciences, 2(3), 93-97. https://doi.org/10.26650/JARHS2019-652926


AMA

Kekik Çınar Ç, Temurhan S, Usta Akgül S, Aydın F. Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy. Journal of Advanced Research in Health Sciences. 2019;2(3):93-97. https://doi.org/10.26650/JARHS2019-652926


ABNT

Kekik Çınar, Ç.; Temurhan, S.; Usta Akgül, S.; Aydın, F. Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 2, n. 3, p. 93-97, 2019.


Chicago: Author-Date Style

Kekik Çınar, Çiğdem, and Sonay Temurhan and Sebahat Usta Akgül and Filiz Aydın. 2019. “Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy.” Journal of Advanced Research in Health Sciences 2, no. 3: 93-97. https://doi.org/10.26650/JARHS2019-652926


Chicago: Humanities Style

Kekik Çınar, Çiğdem, and Sonay Temurhan and Sebahat Usta Akgül and Filiz Aydın. “Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy.” Journal of Advanced Research in Health Sciences 2, no. 3 (May. 2024): 93-97. https://doi.org/10.26650/JARHS2019-652926


Harvard: Australian Style

Kekik Çınar, Ç & Temurhan, S & Usta Akgül, S & Aydın, F 2019, 'Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy', Journal of Advanced Research in Health Sciences, vol. 2, no. 3, pp. 93-97, viewed 19 May. 2024, https://doi.org/10.26650/JARHS2019-652926


Harvard: Author-Date Style

Kekik Çınar, Ç. and Temurhan, S. and Usta Akgül, S. and Aydın, F. (2019) ‘Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy’, Journal of Advanced Research in Health Sciences, 2(3), pp. 93-97. https://doi.org/10.26650/JARHS2019-652926 (19 May. 2024).


MLA

Kekik Çınar, Çiğdem, and Sonay Temurhan and Sebahat Usta Akgül and Filiz Aydın. “Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy.” Journal of Advanced Research in Health Sciences, vol. 2, no. 3, 2019, pp. 93-97. [Database Container], https://doi.org/10.26650/JARHS2019-652926


Vancouver

Kekik Çınar Ç, Temurhan S, Usta Akgül S, Aydın F. Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy. Journal of Advanced Research in Health Sciences [Internet]. 19 May. 2024 [cited 19 May. 2024];2(3):93-97. Available from: https://doi.org/10.26650/JARHS2019-652926 doi: 10.26650/JARHS2019-652926


ISNAD

Kekik Çınar, Çiğdem - Temurhan, Sonay - Usta Akgül, Sebahat - Aydın, Filiz. “Frequency of HLA-Class I Allel in Patients with Spondyloarthropathy”. Journal of Advanced Research in Health Sciences 2/3 (May. 2024): 93-97. https://doi.org/10.26650/JARHS2019-652926



TIMELINE


Submitted29.11.2019
Accepted16.12.2019
Published Online30.12.2019

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.