Journal of Advanced Research in Health Sciences

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DOI :10.26650/JARHS2019-651072   IUP :10.26650/JARHS2019-651072    Full Text (PDF)

Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells

Ayşe Berna Yüzbaşıoğulları, Bilge Şadan Özsait Selçuk, Ayşe Evrim Bayrak, Nihan Erginel Ünaltuna

Differential gene expression is important in the regulation and maintenance of developmental stages and tissue specific physiological processes. The subtractive hybridization method is an efficient method for the detection of genes that have tissue specific expressions. In our previous work, our team had constructed a BALB/c mice heart tissue specific subtractive hybridization cDNA library. Taube Nuss (Tbn), which is a crucial transcription factor, is one of the genes that were isolated from this library. In this study, our aim was to analyze the expression of possible Tbn related genes and identify the Tbn pathway. In the scope of this study, the Tbn gene expression in BALB/c mice heart and skeletal tissue was analyzed with the Northern Blot technique. Then, the Tbn gene was silenced in H9c2 rat cardiomyoblast cell line using siRNA specific to the TBN gene. The gene expression levels of Pparg, Cebpa, Taf10 and Myocd genes, which were possibly related to the Tbn pathway, were analyzed in silenced cells. We observed that the expression of the Myocd gene was decreased, whereas the expression of the Pparg gene was increased in these cells. These results suggest that the Tbn gene plays a regulatory role in cardiac tissue. 

Keywords: siRNA, H9c2, Tbn, Pparg, Taf10
DOI :10.26650/JARHS2019-651072   IUP :10.26650/JARHS2019-651072    Full Text (PDF)

Kardiyomiyoblast Hücre Regülasyonunda Taube Nuss Geninin Fonksiyonel Analizi

Ayşe Berna Yüzbaşıoğulları, Bilge Şadan Özsait Selçuk, Ayşe Evrim Bayrak, Nihan Erginel Ünaltuna

Gelişim evrelerinin veya dokuya özgü fizyolojik süreçlerin düzenlenmesinde ve yürütülmesinde farklılaşmış gen ekspresyonu büyük önem taşımaktadır. “Subtractive” hibridizasyon yöntemi dokuya özgü ekspresyonu olan genlerin tespit edilmesi için oldukça etkin bir yöntemdir. Daha önceki çalışmalarda laboratuvarımızda BALB/c ırkı farelerde kalbe özgü ekspresyonu olan genleri içeren “Subtractive” hibridizasyon cDNA kütüphanesi oluşturulmuştur. Önemli bir transkripsiyon faktörü olan Taube Nuss (Tbn), bu kütüphaneden elde edilen genlerden birisidir. Bu çalışmadaki amacımız, Tbn geni ile ilişkili olduğu düşünülen diğer genlerin ekspresyonlarının araştırılması ve Tbn yolağının belirlenmesidir. Çalışma kapsamında BALB/c ırkı farelerden elde edilen kalp ve iskelet kası dokularına özgü Tbn geninin ekspresyonu Northern Blot analizi ile araştırılmıştır. Ardından, Tbn genine özgü siRNA’lar kullanılarak H9c2 rat kardiyomiyoblast hücre soyunda Tbn geni sessizleştirilmiştir. Yolak analizleri sonucunda Tbn ile ilişkili olabileceği tespit edilen Pparg, Cebpa, Taf10 ve Myocd genlerinin sessizleştirilen hücrelerdeki ekspresyon değişimleri araştırılmıştır. Bu hücrelerde, Myocd gen ekspresyonunun azaldığı ve Pparg gen ekspresyonunun arttığı saptanmıştır. Bu sonuçlar, Tbn geninin kalp dokusunda düzenleyici bir rol oynadığını işaret etmektedir. 

Keywords: siRNA, H9c2, Tbn, Pparg, Taf10

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APA

Yüzbaşıoğulları, A., Özsait Selçuk, B.Ş., Bayrak, A.E., & Ünaltuna, N.E. (2019). Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells. Journal of Advanced Research in Health Sciences, 2(3), 98-104. https://doi.org/10.26650/JARHS2019-651072


AMA

Yüzbaşıoğulları A, Özsait Selçuk B Ş, Bayrak A E, Ünaltuna N E. Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells. Journal of Advanced Research in Health Sciences. 2019;2(3):98-104. https://doi.org/10.26650/JARHS2019-651072


ABNT

Yüzbaşıoğulları, A.; Özsait Selçuk, B.Ş.; Bayrak, A.E.; Ünaltuna, N.E. Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 2, n. 3, p. 98-104, 2019.


Chicago: Author-Date Style

Yüzbaşıoğulları, Ayşe Berna, and Bilge Şadan Özsait Selçuk and Ayşe Evrim Bayrak and Nihan Erginel Ünaltuna. 2019. “Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells.” Journal of Advanced Research in Health Sciences 2, no. 3: 98-104. https://doi.org/10.26650/JARHS2019-651072


Chicago: Humanities Style

Yüzbaşıoğulları, Ayşe Berna, and Bilge Şadan Özsait Selçuk and Ayşe Evrim Bayrak and Nihan Erginel Ünaltuna. “Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells.” Journal of Advanced Research in Health Sciences 2, no. 3 (May. 2024): 98-104. https://doi.org/10.26650/JARHS2019-651072


Harvard: Australian Style

Yüzbaşıoğulları, A & Özsait Selçuk, BŞ & Bayrak, AE & Ünaltuna, NE 2019, 'Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells', Journal of Advanced Research in Health Sciences, vol. 2, no. 3, pp. 98-104, viewed 19 May. 2024, https://doi.org/10.26650/JARHS2019-651072


Harvard: Author-Date Style

Yüzbaşıoğulları, A. and Özsait Selçuk, B.Ş. and Bayrak, A.E. and Ünaltuna, N.E. (2019) ‘Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells’, Journal of Advanced Research in Health Sciences, 2(3), pp. 98-104. https://doi.org/10.26650/JARHS2019-651072 (19 May. 2024).


MLA

Yüzbaşıoğulları, Ayşe Berna, and Bilge Şadan Özsait Selçuk and Ayşe Evrim Bayrak and Nihan Erginel Ünaltuna. “Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells.” Journal of Advanced Research in Health Sciences, vol. 2, no. 3, 2019, pp. 98-104. [Database Container], https://doi.org/10.26650/JARHS2019-651072


Vancouver

Yüzbaşıoğulları A, Özsait Selçuk BŞ, Bayrak AE, Ünaltuna NE. Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells. Journal of Advanced Research in Health Sciences [Internet]. 19 May. 2024 [cited 19 May. 2024];2(3):98-104. Available from: https://doi.org/10.26650/JARHS2019-651072 doi: 10.26650/JARHS2019-651072


ISNAD

Yüzbaşıoğulları, Ayşe Berna - Özsait Selçuk, BilgeŞadan - Bayrak, AyşeEvrim - Ünaltuna, NihanErginel. “Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells”. Journal of Advanced Research in Health Sciences 2/3 (May. 2024): 98-104. https://doi.org/10.26650/JARHS2019-651072



TIMELINE


Submitted26.11.2019
Accepted04.12.2019
Published Online30.12.2019

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