Journal of Advanced Research in Health Sciences

SUBMITINFORMATION
EnglishTürkçe

Research Article


DOI :10.26650/JARHS2019-651072   IUP :10.26650/JARHS2019-651072    Full Text (PDF)

Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells

Ayşe Berna Yüzbaşıoğulları, Bilge Şadan Özsait Selçuk, Ayşe Evrim Bayrak, Nihan Erginel Ünaltuna

Differential gene expression is important in the regulation and maintenance of developmental stages and tissue specific physiological processes. The subtractive hybridization method is an efficient method for the detection of genes that have tissue specific expressions. In our previous work, our team had constructed a BALB/c mice heart tissue specific subtractive hybridization cDNA library. Taube Nuss (Tbn), which is a crucial transcription factor, is one of the genes that were isolated from this library. In this study, our aim was to analyze the expression of possible Tbn related genes and identify the Tbn pathway. In the scope of this study, the Tbn gene expression in BALB/c mice heart and skeletal tissue was analyzed with the Northern Blot technique. Then, the Tbn gene was silenced in H9c2 rat cardiomyoblast cell line using siRNA specific to the TBN gene. The gene expression levels of Pparg, Cebpa, Taf10 and Myocd genes, which were possibly related to the Tbn pathway, were analyzed in silenced cells. We observed that the expression of the Myocd gene was decreased, whereas the expression of the Pparg gene was increased in these cells. These results suggest that the Tbn gene plays a regulatory role in cardiac tissue. 

Keywords: siRNA, H9c2, Tbn, Pparg, Taf10
DOI :10.26650/JARHS2019-651072   IUP :10.26650/JARHS2019-651072    Full Text (PDF)

Kardiyomiyoblast Hücre Regülasyonunda Taube Nuss Geninin Fonksiyonel Analizi

Ayşe Berna Yüzbaşıoğulları, Bilge Şadan Özsait Selçuk, Ayşe Evrim Bayrak, Nihan Erginel Ünaltuna

Gelişim evrelerinin veya dokuya özgü fizyolojik süreçlerin düzenlenmesinde ve yürütülmesinde farklılaşmış gen ekspresyonu büyük önem taşımaktadır. “Subtractive” hibridizasyon yöntemi dokuya özgü ekspresyonu olan genlerin tespit edilmesi için oldukça etkin bir yöntemdir. Daha önceki çalışmalarda laboratuvarımızda BALB/c ırkı farelerde kalbe özgü ekspresyonu olan genleri içeren “Subtractive” hibridizasyon cDNA kütüphanesi oluşturulmuştur. Önemli bir transkripsiyon faktörü olan Taube Nuss (Tbn), bu kütüphaneden elde edilen genlerden birisidir. Bu çalışmadaki amacımız, Tbn geni ile ilişkili olduğu düşünülen diğer genlerin ekspresyonlarının araştırılması ve Tbn yolağının belirlenmesidir. Çalışma kapsamında BALB/c ırkı farelerden elde edilen kalp ve iskelet kası dokularına özgü Tbn geninin ekspresyonu Northern Blot analizi ile araştırılmıştır. Ardından, Tbn genine özgü siRNA’lar kullanılarak H9c2 rat kardiyomiyoblast hücre soyunda Tbn geni sessizleştirilmiştir. Yolak analizleri sonucunda Tbn ile ilişkili olabileceği tespit edilen Pparg, Cebpa, Taf10 ve Myocd genlerinin sessizleştirilen hücrelerdeki ekspresyon değişimleri araştırılmıştır. Bu hücrelerde, Myocd gen ekspresyonunun azaldığı ve Pparg gen ekspresyonunun arttığı saptanmıştır. Bu sonuçlar, Tbn geninin kalp dokusunda düzenleyici bir rol oynadığını işaret etmektedir. 

Keywords: siRNA, H9c2, Tbn, Pparg, Taf10

PDF View

References

  • 1. Calore M., De Windt L.J., Rampazzo A. (2015): Genetics meets epigenetics: genetic variants that modulate noncoding RNA in cardiovascular diseases, J Mol Cell Cardiol, 89: 27-34. google scholar
  • 2. Elia L., Condorelli G. (2015): RNA (Epi)genetics in cardiovascular diseases, J Mol Cell Cardiol, 89: 11-16. google scholar
  • 3. Diatchenko L., Lau Y.F., Campbell A.P., Chenchik A., Moqadam F., Huang B., Lukyanov S., Lukyanov K., Gurskaya N., Sverdlov E.D., Siebert P.D. (1996): Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries, Proc Natl Acad Sci U S A, 93(12): 6025-30. google scholar
  • 4. Özsait B. (2003): “Subtractive” Hibridizasyon Kütüphanesinden izole edilen ve kalp gelişiminde rolü olduğu düşünülen genlerin analizi, Yayınlanmamış Yüksek Lisans Tezi, İstanbul Üniversitesi Sağlık Bilimleri Enstitüsü, İstanbul. google scholar
  • 5. Komurcu-Bayrak E., Ozsait B., Erginel-Unaltuna N. (2012): Isolation and analysis of genes mainly expressed in adult mouse heart using subtractive hybridization cDNA library, Mol Biol Rep,39(8):8065-74. google scholar
  • 6. Ozsait Selcuk B., Kömürcü Bayrak E., Erginal Ünaltuna N. (2016): Higher Expression level of Bat3 is associated with silencing of Midn gene in primary mouse cardiomyocytes, Turk J Biol, 40: 1295-1302. google scholar
  • 7. Guermah M., Ge K., Chiang C.M., Roeder R.G. Guermah M., Ge K., Chiang C.M., Roeder R.G. (2003): The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis, Mol Cell, 12: 991-1001. google scholar
  • 8. Voss A.K., Thomas T., Petrou P., Anastassiadis K., Schöler H., Gruss P. (2000): Taube Nuss is a Novel Gene Essential for the Survival of Pluripotent Cells of Early Mouse Embryos, Development, 127: 5449-61. google scholar
  • 9. Brison D.R., Schultz R.M. (1997): Apoptosis during mouse blastocyst formation: evidence for a role for survival factors including transforming growth factor alpha, Biol Reprod, 56: 1088-96. google scholar
  • 10. Demény M.A., Soutoglou E., Nagy Z., Scheer E., Jànoshàzi A., Richardot M., Argentini M., Kessler P., Tora L. (2007): Identification of a small TAF complex and its role in the assembly of TAF-containing complexes, PLoS One, 2(3):e316. google scholar
  • 11. Trowitzsch S., Viola C., Scheer E., Conic S., Chavant V., Fournier M., Papai G., Ebong I.O., Schaffitzel C., Zou J., Haffke M., Rappsilber J., Robinson C.V., Schultz P., Tora L., Berger I. (2015): Cytoplasmic TAF2-TAF8TAF10 complex provides evidence for nuclear holoTFIID assembly from preformed submodules, Nat Commun, 14(6): 6011. google scholar
  • 12. Tatarakis A., Margaritis T., Martinez-Jimenez C.P., Kouskouti A., Mohan W.S. 2nd, Haroniti A., Kafetzopoulos D., Tora L., Talianidis I.. (2008): Dominant and redundant functions of TFIID involved in the regulation of hepatic genes, Mol Cell, 31: 531–543. google scholar
  • 13. Mouse ENCODE Consortium. (2014): A comparative encyclopedia of DNA elements in the mouse genome, Nature, 515(7527):355-64. google scholar
  • 14. El-Jack A.K., Hamm J.K., Pilch P.F., Farmer S.R.. (1999): Reconstitution of Insulin-sensitive Glucose Transport in Fibroblasts Requires Expression of Both PPARγ and C/EBPα, J Biol Chem, 274: 7946-51. google scholar
  • 15. Gordon J.W. (2018): Regulation of cardiac myocyte cell death and differentiation by myocardin, Mol Cell Biochem, 437(1-2): 119-131. google scholar
  • 16. Raphel L., Talasila A., Cheung C., Sinha S. (2012): Myocardin overexpression is sufficient for promoting the development of a mature smooth muscle cell-like phenotype from human embryonic stem cells, PLoS One, 7(8): e44052. google scholar
  • 17. Singh J., Verma N.K., Kansagra S.M., Kate B.N. Dey C.S. (2007): Altered PPARgamma expression inhibits myogenic differentiation in C2C12 skeletal muscle cells, Molecular and Cellular Biochemistry, 294: 163–171. google scholar
  • 18. He K., Wu G., Li W.X., Guan D., Lv W., Gong M., Ye S., Lu A. (2017): A transcriptomic study of myogenic differentiation under the overexpression of PPARγ by RNA-Seq, Sci Rep, 7(1):15308. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Yüzbaşıoğulları, A., Özsait Selçuk, B.Ş., Bayrak, A.E., & Ünaltuna, N.E. (2019). Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells. Journal of Advanced Research in Health Sciences, 2(3), 98-104. https://doi.org/10.26650/JARHS2019-651072


AMA

Yüzbaşıoğulları A, Özsait Selçuk B Ş, Bayrak A E, Ünaltuna N E. Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells. Journal of Advanced Research in Health Sciences. 2019;2(3):98-104. https://doi.org/10.26650/JARHS2019-651072


ABNT

Yüzbaşıoğulları, A.; Özsait Selçuk, B.Ş.; Bayrak, A.E.; Ünaltuna, N.E. Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 2, n. 3, p. 98-104, 2019.


Chicago: Author-Date Style

Yüzbaşıoğulları, Ayşe Berna, and Bilge Şadan Özsait Selçuk and Ayşe Evrim Bayrak and Nihan Erginel Ünaltuna. 2019. “Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells.” Journal of Advanced Research in Health Sciences 2, no. 3: 98-104. https://doi.org/10.26650/JARHS2019-651072


Chicago: Humanities Style

Yüzbaşıoğulları, Ayşe Berna, and Bilge Şadan Özsait Selçuk and Ayşe Evrim Bayrak and Nihan Erginel Ünaltuna. “Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells.” Journal of Advanced Research in Health Sciences 2, no. 3 (Dec. 2024): 98-104. https://doi.org/10.26650/JARHS2019-651072


Harvard: Australian Style

Yüzbaşıoğulları, A & Özsait Selçuk, BŞ & Bayrak, AE & Ünaltuna, NE 2019, 'Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells', Journal of Advanced Research in Health Sciences, vol. 2, no. 3, pp. 98-104, viewed 22 Dec. 2024, https://doi.org/10.26650/JARHS2019-651072


Harvard: Author-Date Style

Yüzbaşıoğulları, A. and Özsait Selçuk, B.Ş. and Bayrak, A.E. and Ünaltuna, N.E. (2019) ‘Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells’, Journal of Advanced Research in Health Sciences, 2(3), pp. 98-104. https://doi.org/10.26650/JARHS2019-651072 (22 Dec. 2024).


MLA

Yüzbaşıoğulları, Ayşe Berna, and Bilge Şadan Özsait Selçuk and Ayşe Evrim Bayrak and Nihan Erginel Ünaltuna. “Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells.” Journal of Advanced Research in Health Sciences, vol. 2, no. 3, 2019, pp. 98-104. [Database Container], https://doi.org/10.26650/JARHS2019-651072


Vancouver

Yüzbaşıoğulları A, Özsait Selçuk BŞ, Bayrak AE, Ünaltuna NE. Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells. Journal of Advanced Research in Health Sciences [Internet]. 22 Dec. 2024 [cited 22 Dec. 2024];2(3):98-104. Available from: https://doi.org/10.26650/JARHS2019-651072 doi: 10.26650/JARHS2019-651072


ISNAD

Yüzbaşıoğulları, Ayşe Berna - Özsait Selçuk, BilgeŞadan - Bayrak, AyşeEvrim - Ünaltuna, NihanErginel. “Functional Analysis of Taube Nuss Gene in the Regulation of Cardiomyoblast Cells”. Journal of Advanced Research in Health Sciences 2/3 (Dec. 2024): 98-104. https://doi.org/10.26650/JARHS2019-651072



TIMELINE


Submitted26.11.2019
Accepted04.12.2019
Published Online30.12.2019

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.