Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children

Karaaslan, Ayşe; Ulger Toprak, Nurver; Akkoç, Gülşen; Akgül, Öncü; Kepenekli Kadayifci, Eda; Öcal Demir, Sevliya; Yakut, Nurhayat; Atıcı, Serkan; Soysal, Ahmet; Bakır, Mustafa; Söyletir, Güner

doi:https://dx.doi.org/10.5222/j.child.2017.169

Research Article

DOI :10.5222/j.child.2017.169 IUP :10.5222/j.child.2017.169 Full Text (PDF)

Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children

Ayşe Karaaslan, Nurver Ulger Toprak, Gülşen Akkoç, Öncü Akgül, Eda Kepenekli Kadayifci, Sevliya Öcal Demir, Nurhayat Yakut, Serkan Atıcı, Ahmet Soysal, Mustafa Bakır, Güner Söyletir

Objective: The aim of this study was to investigate the carbapenem resistance profiles and risk factors for colonization with carbapenem-resistant Bacteroides group (CR-B) in pediatric patients hospitalized in a tertiary university hospital in Turkey. Material and Methods: A prospective case-control study was performed. Rectal swab specimens (n=1361) were collected from 680 hospitalized pediatric patients. All anaerobic colonies isolated from selective medium were identified using MALDI-TOF (Vitek MS, bioMérieux) automated system and antibiotic susceptibility testing for meropenem by e-test. The presence of the cfiA gene was investigated by PCR method. Results: A total of 1361 rectal swab specimens were collected from all 680 hospitalized children. During the screening period 80 Bacteroides fragilis group (BFG) microorganisms with a MIC>0.125 mg/L (12%) were isolated from rectal swab specimens of 680 patients. Seven different BFG species were recovered, the main species were B.fragilis (n=29; 36%), B.ovatus (n=16; 20%), B.vulgatus (n=13; 16%), P.distasonis (n=12; 15%), B.theoiotomicron (n=5; 6%), B.uniformis (n=4; 5%) and B.caccae (n=1; 1%). The presence of cfiA gene was detected in 25 (31%) isolates, while 29 (36%) isolates were not susceptible to meropenem (MIC>2 mg/L). Conclusion: The high carriage rate in our hospitalized children for carbapenem-non-susceptible Bacteroides fragilis group creates a great risk for serious infections and mortality and deserves significant attention.

Keywords: Bacteroides fragilis group, carbapenem nonsusceptible, children

DOI :10.5222/j.child.2017.169 IUP :10.5222/j.child.2017.169 Full Text (PDF)

Hastanede Yatan Çocukların Rektal Sürüntülerinde Karbapenem Duyarlı Olmayan Bacteroides fragilis Grup Bakteri Taraması

Ayşe Karaaslan, Nurver Ulger Toprak, Gülşen Akkoç, Öncü Akgül, Eda Kepenekli Kadayifci, Sevliya Öcal Demir, Nurhayat Yakut, Serkan Atıcı, Ahmet Soysal, Mustafa Bakır, Güner Söyletir

Amaç: Bu çalışmanın amacı, Türkiye’de üçüncü düzey bir üniversite hastanesinde yatmakta olan çocuk hastalarda karbapenem dirençli Bacteroides grup (KD-B) bakteriler ile kolonizasyon için risk faktörlerini ve karbapenem direnç profilini araştırmaktı. Gereç ve Yöntem: Prospektif vaka-kontrol çalışması uygulandı. Hastanede yatmakta olan 680 çocuk hastadan 1361 rektal swab toplandı. Selektif mediumdan izole edilen tüm anaerobik kolonilere identifikasyon için MALDI-TOF (Vitek MS, bioMérieux) otomatize sistem ve meropenem duyarlılığı için e-test uygulandı. cfiA geni PCR yöntemiyle araştırıldı. Bulgular: Hastanede yatmakta olan 680 çocuk hastadan 1361 rektal swab toplandı. Tarama süresince MIC>0.125 mg/L olan 80 Bacteroides fragilis group (BFG) bakteri izole edildi. Yedi farklı tür BFG saptandı, sıklık sırasına göre şöyleydi: B.fragilis (n=29; %36), B.ovatus (n=16; %20), B.vulgatus (n=13; %16), P.distasonis (n=12; %15), B.theoiotomicron (n=5; %6), B.uniformis (n=4; %5) ve B.caccae (n=1; %1). 25 izolatda (%31) cfiA geni saptanırken, meropeneme hassas olmama (MIC>2 mg/L) 29 izolatda (%36) belirlendi. Sonuç: Çalışmamızda, hastanede yatan çocuklarda karbapenem hassas olmayan Bacteroides fragilis grup bakteriler ile kolonizasyon oranları yüksek bulunmuştur ki, bu durum ciddi enfeksiyonların oluşması, artmış mortalite açısından önemlidir ve dikkate alınmalıdır.

Keywords: Bacteroides fragilis grup, karbapenem hassas olmama, çocuklar

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APA

Karaaslan, A., Ulger Toprak, N., Akkoç, G., Akgül, Ö., Kepenekli Kadayifci, E., Öcal Demir, S., Yakut, N., Atıcı, S., Soysal, A., Bakır, M., & Söyletir, G. (2017). Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children. Journal of Child, 17(4), 169-173. https://doi.org/10.5222/j.child.2017.169

AMA

Karaaslan A, Ulger Toprak N, Akkoç G, Akgül Ö, Kepenekli Kadayifci E, Öcal Demir S, Yakut N, Atıcı S, Soysal A, Bakır M, Söyletir G. Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children. Journal of Child. 2017;17(4):169-173. https://doi.org/10.5222/j.child.2017.169

ABNT

Karaaslan, A.; Ulger Toprak, N.; Akkoç, G.; Akgül, Ö.; Kepenekli Kadayifci, E.; Öcal Demir, S.; Yakut, N.; Atıcı, S.; Soysal, A.; Bakır, M.; Söyletir, G. Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children. Journal of Child, [Publisher Location], v. 17, n. 4, p. 169-173, 2017.

Chicago: Author-Date Style

Karaaslan, Ayşe, and Nurver Ulger Toprak and Gülşen Akkoç and Öncü Akgül and Eda Kepenekli Kadayifci and Sevliya Öcal Demir and Nurhayat Yakut and Serkan Atıcı and Ahmet Soysal and Mustafa Bakır and Güner Söyletir. 2017. “Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children.” Journal of Child 17, no. 4: 169-173. https://doi.org/10.5222/j.child.2017.169

Chicago: Humanities Style

Karaaslan, Ayşe, and Nurver Ulger Toprak and Gülşen Akkoç and Öncü Akgül and Eda Kepenekli Kadayifci and Sevliya Öcal Demir and Nurhayat Yakut and Serkan Atıcı and Ahmet Soysal and Mustafa Bakır and Güner Söyletir. “Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children.” Journal of Child 17, no. 4 (Apr. 2025): 169-173. https://doi.org/10.5222/j.child.2017.169

Harvard: Australian Style

Karaaslan, A & Ulger Toprak, N & Akkoç, G & Akgül, Ö & Kepenekli Kadayifci, E & Öcal Demir, S & Yakut, N & Atıcı, S & Soysal, A & Bakır, M & Söyletir, G 2017, 'Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children', Journal of Child, vol. 17, no. 4, pp. 169-173, viewed 4 Apr. 2025, https://doi.org/10.5222/j.child.2017.169

Harvard: Author-Date Style

Karaaslan, A. and Ulger Toprak, N. and Akkoç, G. and Akgül, Ö. and Kepenekli Kadayifci, E. and Öcal Demir, S. and Yakut, N. and Atıcı, S. and Soysal, A. and Bakır, M. and Söyletir, G. (2017) ‘Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children’, Journal of Child, 17(4), pp. 169-173. https://doi.org/10.5222/j.child.2017.169 (4 Apr. 2025).

MLA

Karaaslan, Ayşe, and Nurver Ulger Toprak and Gülşen Akkoç and Öncü Akgül and Eda Kepenekli Kadayifci and Sevliya Öcal Demir and Nurhayat Yakut and Serkan Atıcı and Ahmet Soysal and Mustafa Bakır and Güner Söyletir. “Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children.” Journal of Child, vol. 17, no. 4, 2017, pp. 169-173. [Database Container], https://doi.org/10.5222/j.child.2017.169

Vancouver

Karaaslan A, Ulger Toprak N, Akkoç G, Akgül Ö, Kepenekli Kadayifci E, Öcal Demir S, Yakut N, Atıcı S, Soysal A, Bakır M, Söyletir G. Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children. Journal of Child [Internet]. 4 Apr. 2025 [cited 4 Apr. 2025];17(4):169-173. Available from: https://doi.org/10.5222/j.child.2017.169 doi: 10.5222/j.child.2017.169

ISNAD

Karaaslan, Ayşe - Ulger Toprak, Nurver - Akkoç, Gülşen - Akgül, Öncü - Kepenekli Kadayifci, Eda - Öcal Demir, Sevliya - Yakut, Nurhayat - Atıcı, Serkan - Soysal, Ahmet - Bakır, Mustafa - Söyletir, Güner. “Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children”. Journal of Child 17/4 (Apr. 2025): 169-173. https://doi.org/10.5222/j.child.2017.169

Volume 17, Issue 42017, P. 169-173

TIMELINE

Submitted	23.11.2017
Accepted	26.01.2018

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Journal of Child

Research Article

Screening of Rectal Swabs for Carbapenem Non-susceptible Bacteroides fragilis Group Bacteria in Hospitalized Children

Hastanede Yatan Çocukların Rektal Sürüntülerinde Karbapenem Duyarlı Olmayan Bacteroides fragilis Grup Bakteri Taraması

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