Journal of Istanbul Faculty of Medicine

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DOI :10.26650/IUITFD.1458482   IUP :10.26650/IUITFD.1458482    Full Text (PDF)

EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER

Zeynep Akbulut, Beste Erdem, Hatice Maraş, Gamze Demirel, Ranan Gülhan Aktaş

Objective: In cancer research, studies showing the behaviour of cells in different environments is important for developing new treatment methods. The microenvironment is essential for both regular and cancerous tissues. The main component of the extracellular matrix (ECM) is collagen. In HepG2 cells, a hepatocellular carcinoma cell line, the effects of Type 1 and Type 3 collagen on cell proliferation and the expression of alpha-feto protein (AFP), which is an indicator of carcinogenicity, was examined. 

Material and Method: HepG2 cells were grown in Type 1 or Type 3 covered surfaces, whereas no treatment was applied to the control group. Proliferation analysis was performed via microscopic examination and cell viability assessmet kit (Cell Counting Kit-8, CCK-8). AFP was measured using a confocal microscope using immunological staining.

Result: The results showed that the viability rate of HepG2 cells growing in Type 3 collagen medium was statistically higher than in the control group (p<0.0001). AFP expression increased significantly in the presence of Type 3 collagen compared with the control group at the 24th hour (p<0.05), and at other culture times, AFP expression was seen more in Type 1 collagen culture medium.  

Conclusion: HepG2 cells cultured in both types of collagen have a morphologically similar structure, and Type 1 and Type 3 collagen have a proliferation-enhancing effect on cancer cells. AFP, an indicator of liver cancer, is high in culture media containing Type 1 and Type 3 collagen and this finding is considered as the tendency of bad prognosis of collagen types on AFP expression.

Keywords: Alpha-fetoprotein, Extracellular matrix, Hepatocellu lar carcinoma, Type 1 collagen, Type 3 collagen
DOI :10.26650/IUITFD.1458482   IUP :10.26650/IUITFD.1458482    Full Text (PDF)

EKSTRASELLÜLER MATRİKS BİLEŞENLERİNDEN TİP 1 VE TİP 3 KOLLAJENİN KARACİĞER KANSERİ İNSAN HÜCRE HATTI MODELİNDE HÜCRE PROLİFERASYONUNA VE ALFA FETO PROTEİN EKSPRESYONU ÜZERİNE ETKİLERİ

Zeynep Akbulut, Beste Erdem, Hatice Maraş, Gamze Demirel, Ranan Gülhan Aktaş

Amaç: Kanser araştırmalarında, hücrelerin farklı ortamlardaki davranışlarını gösteren çalışmalar yeni tedavi yöntemlerinin geliştirilmesi için büyük önem taşımaktadır. Mikroçevre hem normal doku hem de kanserli doku için çok önemlidir. Ekstraselüler matriks (ECM) ana bileşenleri kollajenlerdir. Hepatosellüler karsinom hücre hattı olan HepG2 hücrelerinde Tip 1 ve Tip 3 kollajenin hücre proliferasyonu ve karsinojenite göstergesi olan alfa-feto protein (AFP) ekspresyonu üzerine etkisi incelenmiştir. 

Gereç ve Yöntem: Proliferasyon analizi mikroskobik inceleme ve hücre canlılığı değerlendirme kiti (Cell Counting Kit-8, CCK-8) ile yapıldı. AFP ölçümleri immünolojik boyama yöntemleri kullanıla rak konfokal mikroskop ile yapıldı.

Bulgular: Sonuçlar, Tip 3 kollajen ortamında büyüyen HepG2 hücrelerinin canlılık oranının kontrol grubuna göre istatistiksel olarak daha yüksek olduğunu gösterdi (p<0,0001). AFP ekspre syonu 24. saatte kontrol grubuna kıyasla Tip 3 kollajen varlığında anlamlı olarak artmış (p<0,05), diğer kültür zamanlarında ise AFP ekspresyonu Tip 1 kollajen kültür ortamında daha fazla görülmüştür.

Sonuç: Her iki tip kollajende kültüre edilen HepG2 hücrelerinin morfolojik olarak benzer bir yapıya sahip olduğu, Tip I kollajen ve Tip 3 kollajenin kanser hücreleri üzerinde proliferasyonu artırıcı etkiye sahip olduğu belirlenmiştir. Karaciğer kanserinin göstergelerinden biri olan AFP, Tip 1 kollajen ve Tip 3 kollajen içeren kültür ortamlarında yüksek bulunmuş, bunun da kollajen tiplerinin AFP ekspresyonu üzerinde kötü prognoza doğru bir eğilim gösterdiğini düşündürmektedir.

Keywords: Alfa fetoprotein, Ekstrasellüler matriks, He patosellüler karsinoma, Tip 1 kollajen, Tip 3 kollajen

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References

  • Chuang SC, La Vecchia C, Boffetta P. Liver cancer: descriptive epidemiology and risk factors other than HBV and HCV infection. Cancer Lett 2009;286:9-14. [CrossRef] google scholar
  • Stuver S, Trichopoulos D. Cancer of the Liver and Biliary Tract. In: Adami H, Hunter D, Trichopoulos D, eds. Textbook of Cancer 2008. [CrossRef] google scholar
  • Yang X, Chen L, Liang Y, Si R, Jiang Z, Ma B, Gao P. Knockdown of alphafetoprotein expression inhibits HepG2 cell growth and induces apoptosis. J Can Res Ther 2018;14(3):634-43. [CrossRef] google scholar
  • Taş A, Hepatosellüler Karsinom Tanı ve Tedavisi. Güncel Gastroenteroloji 2010;14(3):145-8. google scholar
  • Obuz F. Hepatosellüler karsinomda radyolojik tanı ve evreleme. Trd sem. 2015;3:437-60. [CrossRef] google scholar
  • Sugano S, Miyoshi K, Suzuki T, Kawafune T, Kubota M. Intrahepatic arteriovenous shunting due to the hepatocellular carcinoma and cirrhosis, and its change by transcatheter arterial embolization. Am J Gastroenterol 1994;89(2):184-8. google scholar
  • El-Serag Hb, Rudolph Kl. Gastroenterology 2007;132(7):2557-76. [CrossRef] google scholar
  • Davila JA, Mogan RO, Shaib Y, McGlynn KA, El-Serag HB. Hepatitis C infection and the creasing incidence of hepatocellular carcinoma: A population- based study. Gastroenterology 2004;127(5):1372-80. [CrossRef] google scholar
  • Pickup MW, Mouw JK, Weaver VM. The extracellular matrix modulates the hallmarks of cancer. EMBO Rep 2014;15(12):1243-53. [CrossRef] google scholar
  • Moghe PV, Berthiaume F, Ezzell RM, Toner M, Tompkins RG, Yarmush ML. Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials 1996;17(3):373-85. [CrossRef] google scholar
  • Bager CL, Willumsen N, Leeming DJ, Smith V, Karsdal MA, Dornan D, et al. Collagen degradation products measured in serum can separate ovarian and breast cancer patients from healthy controls: a preliminary study. Cancer Biomark 2015;15(6):783-8. [CrossRef] google scholar
  • Willumsen N, Bager CL, Leeming DJ, Smith V, Karsdal MA, Dornan D, et al. Extracellular matrix specific protein fingerprints measured in serum can seperate pancreatic cancer patients from healthy controls. BMC Cancer 2013;13:554. [CrossRef] google scholar
  • Kauppila S, Stenback F, Risteli J, Jukkola A, Risteli L. Aberrant type I and type III collagen gene expression in human breast cancer in vivo. J Pathol 1998;186(3):262-8. [CrossRef] google scholar
  • Hilska M, Collan Y, Peltonen J, Gullichsen R, Paajanen H, Laato M. The Distribution of Collagen Types I, III, and IV in Normal and Malignant Colorectal Mucosa 1998;164(6):457-64. [CrossRef] google scholar
  • Jensen C, Madsen D, Hansen M, Schmidt H, Svane I, Karsdal M, et al. Altered type III collagen turnover measured in pre treatment serum predicts outcome in metastatic melanoma patients treated with Ipilimumab. Eur J Cancer 2018;92(1):7. [CrossRef] google scholar
  • Menke A, Philippi C, Vogelmann R, Seidel V, Lutz MP, Adler G, et al. Down-regulation of E-cadherin gene expression by collagen type I and type III in pancreatic cancer cell lines. Cancer Res 2001;61(8):3508-17. google scholar
  • Chintala S, Sawaya R, Gokaslan ZL, Rao JS. The effect of type III collagen on migration and invasion of human. Cancer Lett 1996;102:57-63. [CrossRef] google scholar
  • Hirai K, Shimada H, Ogawa T, Taji S. The spread of human lung cancer cells on collagens and its inhibition by type III collagen. Clin Exp Metastasis 1991;9(6):517-27. [CrossRef] google scholar
  • Wang L, Sun Y, Guo Z, Liu H. COL3A1 Overexpression Associates with Poor Prognosis and Cisplatin Resistance in Lung Cancer. Balkan Med J 2022;39(6):393-400. [CrossRef] google scholar
  • Bhattacharjee S, Hamberger F, Ravichandra A, Miller M, Nair A, Affo S, et al. Tumor restriction by type I collagen opposes tumor-promoting effects of cancer-associated f ibroblasts. J Clin Invest 2021;131(11):e146987. [CrossRef] google scholar
  • Badaoui M, Mimsy-Julienne C, Saby C, Van Gulick L, Peretti M, Jeannesson P, et al. Collagen type 1 promotes survival of human breast cancer cells by overexpressing Kv10.1 potassium and Orai1 calcium channels through DDR1 dependent pathway. Oncotarget 2017;9(37):24653-71. [CrossRef] google scholar
  • Brisson BK, Mauldin EA, Lei W, Vogel LK, Power MA, Lo A, et al. Type III collagen directs stromal organization and limits metastasis in a murine model of breast cancer. Am J Pathol 2015;185(5):1471-86. [CrossRef] google scholar

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APA

Akbulut, Z., Erdem, B., Maraş, H., Demirel, G., & Aktaş, R.G. (2024). EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER. Journal of Istanbul Faculty of Medicine, 87(4), 283-290. https://doi.org/10.26650/IUITFD.1458482


AMA

Akbulut Z, Erdem B, Maraş H, Demirel G, Aktaş R G. EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER. Journal of Istanbul Faculty of Medicine. 2024;87(4):283-290. https://doi.org/10.26650/IUITFD.1458482


ABNT

Akbulut, Z.; Erdem, B.; Maraş, H.; Demirel, G.; Aktaş, R.G. EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER. Journal of Istanbul Faculty of Medicine, [Publisher Location], v. 87, n. 4, p. 283-290, 2024.


Chicago: Author-Date Style

Akbulut, Zeynep, and Beste Erdem and Hatice Maraş and Gamze Demirel and Ranan Gülhan Aktaş. 2024. “EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER.” Journal of Istanbul Faculty of Medicine 87, no. 4: 283-290. https://doi.org/10.26650/IUITFD.1458482


Chicago: Humanities Style

Akbulut, Zeynep, and Beste Erdem and Hatice Maraş and Gamze Demirel and Ranan Gülhan Aktaş. “EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER.” Journal of Istanbul Faculty of Medicine 87, no. 4 (Dec. 2024): 283-290. https://doi.org/10.26650/IUITFD.1458482


Harvard: Australian Style

Akbulut, Z & Erdem, B & Maraş, H & Demirel, G & Aktaş, RG 2024, 'EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER', Journal of Istanbul Faculty of Medicine, vol. 87, no. 4, pp. 283-290, viewed 23 Dec. 2024, https://doi.org/10.26650/IUITFD.1458482


Harvard: Author-Date Style

Akbulut, Z. and Erdem, B. and Maraş, H. and Demirel, G. and Aktaş, R.G. (2024) ‘EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER’, Journal of Istanbul Faculty of Medicine, 87(4), pp. 283-290. https://doi.org/10.26650/IUITFD.1458482 (23 Dec. 2024).


MLA

Akbulut, Zeynep, and Beste Erdem and Hatice Maraş and Gamze Demirel and Ranan Gülhan Aktaş. “EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER.” Journal of Istanbul Faculty of Medicine, vol. 87, no. 4, 2024, pp. 283-290. [Database Container], https://doi.org/10.26650/IUITFD.1458482


Vancouver

Akbulut Z, Erdem B, Maraş H, Demirel G, Aktaş RG. EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER. Journal of Istanbul Faculty of Medicine [Internet]. 23 Dec. 2024 [cited 23 Dec. 2024];87(4):283-290. Available from: https://doi.org/10.26650/IUITFD.1458482 doi: 10.26650/IUITFD.1458482


ISNAD

Akbulut, Zeynep - Erdem, Beste - Maraş, Hatice - Demirel, Gamze - Aktaş, RananGülhan. “EFFECTS OF COLLAGEN TYPE 1 AND 3 ON CELL PROLIFERATION AND ALPHA FETO PROTEIN EXPRESSION IN A HUMAN CELL LINE MODEL OF LIVER CANCER”. Journal of Istanbul Faculty of Medicine 87/4 (Dec. 2024): 283-290. https://doi.org/10.26650/IUITFD.1458482



TIMELINE


Submitted27.03.2024
Accepted25.06.2024
Published Online03.09.2024

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