Research Article


DOI :10.18017/iuitfd.315519   IUP :10.18017/iuitfd.315519    Full Text (PDF)

EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES

Özden Büyükbaba BoralDeniz Gözde Çelik PayçuRaim İliazAkın AkgülHalim İşseverFiliz Akyüz

Objective: Blastocystis spp; is a protozoon that can be seen worldwide. Fecal-oral transmission is the most accepted pathway for Blastocystis spp. infection. Most infected patients show no symptoms of infection, however it can cause abdominal pain, acute or chronic diarrhea, vomiting, flatulence and nausea. It is suggested that Blastocystis spp. may cause several pathologies in gut causing an inflammatory response that may lead to Inflammatory Bowel Diseases. Materials and Methods: Our study included 60 IBH patients diagnosed as ulcerative colitis (30) and Crohn’s Disease (30) in Division of Gastroenterology and 40 healty controls with no gastrointestinal disease. Stool samples of all patients were examined for presence of any parasites. Presenceof Blastocystis spp. was evaluated with microscopy, culture with two different media, ELISA and PCR methods. For subtype analyses sequencing was performed. Results: Blastocystis spp. was detected in 6 (10%) IBD patients, 2 (5%) in controls. Blastocystis spp. antigen detected in 12 (20%) IBD patients and 3 (7.5%) in controls. Blastocystis spp. DNA was detected in 8 (13.3%) IBD patients and 6 (15%) controls. No statistical significance was detected between IBD patients and control group in terms of Blastocystis spp positivity. ST3 was the predominant subtype and abdominal pain was the most common clinical symptom. Conclusion: These results suggested that using two different media increases the sensitivity. PCR was the most sensitive method. ELISA results showed high sensitivity and specificity, suggested that ELISA method could be an alternative to the microscopy and culture. No significant relationship between IBD and Blastocystis spp. was determined. Also, no significance was detected between subtypes and clinical symptoms. Although we could not determine a relationship between subtypes and IBD or clinical symptoms, further investigations are needed to clarify this issue. 

DOI :10.18017/iuitfd.315519   IUP :10.18017/iuitfd.315519    Full Text (PDF)

İNFLAMATUAR BARSAK HASTALIĞI OLAN HASTALARDA BLASTOCYSTIS SPP.'NİN FARKLI TANI YÖNTEMLERİ İLE ARAŞTIRILMASI VE GENOTİPLENDİRİLMESİ

Özden Büyükbaba BoralDeniz Gözde Çelik PayçuRaim İliazAkın AkgülHalim İşseverFiliz Akyüz

Amaç: Blastocystis spp; fekal-oral yolla özellikle kötü hijyen koşullarında bulaşan, tüm dünyada yaygın bir protozoondur. Enfeksiyon asemptomatik seyredebildiği gibi karın ağrısı, kabızlık, bulantı gibi gastrointestinal semptomlara neden olabilmektedir. Blastocystis spp.’nin barsakta çeşitli patolojilere neden olabileceği, inflamatuar yanıtı uyararak İnflamatuar Barsak Hastalıkları (IBH)’nın gelişmesi ile ilişkisi olabileceği öne sürülmektedir. Gereç-Yöntem: Çalışmamıza İ.Ü. İstanbul Tıp Fakültesi Gastroenteroloji Bilim Dalı’nda 30 Crohn hastalığı (CH), 30 ülseratif kolit (UK) tanısı alan hasta ve 40 gastrointestinal system hastalığı bulunmayan sağlıklı kontrol grubu dahil edilmiştir. Bu hastaların dışkı örnekleri tüm parazitler yönünden incelenmiş, mikroskopi, kültür, ELISA ve PCR ile Blastocystis spp. varlığı araştırılmıştır. Blastocystis spp. saptanan örneklerde subtip dağılımı sekanslama yöntemi ile araştırılmıştır. Bulgular: İBH grubunda mikroskopi ve kültür yöntemleri ile 6 (%10)’sında, kontrol grubunun 2 (%5)’sinde Blastocystis spp. varlığı saptanmıştır. ELISA ile Blastocystis spp. antijeni IBH grubunun 12 (%20)’sinde, kontrol grubunun 3 (%7,5)’ünde saptanmıştır. Blastocystis spp. DNA’sı PCR ile IBH grubunun 8 (%13,3)’inde, kontrol grubunun 6 (15%)’sında bulunmuştur. Gruplar arasında Blastocystis spp. varlığı yönünden anlamlı bir sonuç saptanamamıştır. Suptipler arasında ST3 en sık olarak saptanmış, bunlarda en yaygın klinik semptomun karın ağrısı olduğu gözlenmiştir. Sonuç: İki farklı besiyeri kullanılması tanı duyarlılığını arttırmıştır. En duyarlı tanı yöntemi PCR olarak saptanmış, ELISA yöntemi de oldukça yüksek duyarlılık ve özgüllükte sonuç vermiş, mikroskopi ve kültüre alternatif bir yöntem olabileceği gözlenmiştir. Sonuçlarımız subtipler ile klinik bulgular arasında bir bağlantı olduğunu göstermese de konuyu aydınlatmak için çalışmalara ihtiyaç olduğuna işaret etmektedir.


PDF View

References

  • 1. Popruk P, Pintong A, Radomyos P. Diversity of Blastocystis subtypes in humans. J Trop Med Parasitol 2013; 36:88-97. google scholar
  • 2. Dağcı.H: Blastocystis: Türkiye’deki Son Gelişmeler. 18. Ulusal Parazitoloji Kongresi Kitapçığı, 29 Eylül - 5 Ekim 2013, Denizli, 2013;30-4. google scholar
  • 3. Wawrzyniak I, Poirier P, Viscogliosi E, Dionigia M, Texier C, Delbac F, El-Alaoui H. Blastocystis, an unrecognized parasite:an overview of pathogenesis and diagnosis. Ther Adv Infect Dis 2013; 1:167-78. google scholar
  • 4. Koltas IS, Özcan K, Tanriverdi S, Paydas S, Paydas S, Baslamisli F. The prevalence of Blastocystis hominis in immunosupressed patients. Ann Med Sci 1999; 8:117- 9. google scholar
  • 5. Tasova Y., Sahin B., Koltas S., Paydas S.: Clinical significance and frequency of Blastocystis hominis in Turkish patients with hematological malignancy. Acta Med Okayama 2000; 54:133-6. google scholar
  • 6. Zoglool DAM., Khodari YAW., Frooq MU.: Blastocystis hominis and allergic skin diseases; a single center experience. Journal Of Health Sciences 2012; 2 : 66 google scholar
  • 7. Horiki N, Kaneda Y, Maruyama M, Fujita Y, Tachibana H. Intestinal blockage by carcinoma and Blastocystis hominis infection. Am J Trop Med Hyg 1999; 60:400-2. google scholar
  • 8. Yakoob J., Jafri W., Jafri N., Khan R., Islam M., Beg MA., Zaman V.: Irritable bowel syndrome: in search of an etiology: role of Blastocystis hominis. Am J Trop Med Hyg 2004; 70: 383-5. google scholar
  • 9. Nagler J.,Brown M., Soave R.: Blastocystis hominis in inflammatory bowel disease. J Clin Gastroenterol 1992; 16:109-12. google scholar
  • 10. Giacometti A., Cirioni O., Fiorentini A., Fortuna M., Scalise G.: Irritable bowel syndrome in patients with Blastocystis hominis infection. Eur J Clin Microbiol Infect Dis 1999;18:436-9. google scholar
  • 11. Yakoob J., Jafri W., Beg MA., Abbas Z., Naz S., Islam M., Khan R.: Irrıtable Bowl Sendrome: It is associated with genotypes of Blastocystis hominis. Parasitol Res 2010; 106:1033-8. google scholar
  • 12. Phillips BP, Zierdt CH.: Blastocystis hominis: Pathogenic potential in human patients and in gnotobiotics. Exp Parasitol 1976;39: 358-64. google scholar
  • 13. Long HY., Handschack A., Konig W., Ambrosch A.: Blastocytis hominis modulates immune responses and cytokine release in colonic epithelial cells. Parasitol Res 2001; 87:1029-30. google scholar
  • 14. Alfellani MA, Mulla DT, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG. Genetic diversity of Blastocystis in livestock and zoo animals. Protist 2013;164:497-509. google scholar
  • 15. Noël C, Dufernez F, Gerbod D, Edgcomb VP, Delgado-Viscogliosi P, Ho L.C, Singh M, Wintjens R, Sogin ML, Capron M, Pierce R, Zenner L, Viscogliosi E. Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J Clin Microbiol 2005; 43: 348-55. google scholar
  • 16. Tan KS. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 2008; 21:639-65. google scholar
  • 17. Scanlan PD, Marchesi JR. Micro-eukaryotic diversity of the human distal gut microbiota: quantitative assessment using culture-dependent and independent analysis of faeces. ISME J 2008; 2:1183-93 google scholar
  • 18. Dominguez-Marquez M, Guna R, Munoz C, Gomez-Munoz M, Borras R. High prevalence of subtip 4 among isolates of Blastocystis hominis from symptomatic patients of health district of Valencia (Spain). Parasitol Res 2009; 105:949-55. google scholar
  • 19. Stendsvolt C, Christiansen D, Olsen K, Nielsen H. Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea. Am J Trop Med Hyg 2011; 84:883-5. google scholar
  • 20. Zaman V, Ng GC, Suresh K, Yap EH, Singh M. Isolation of Blastocystis from the cockroach (Dictyoptera, Blattidae). Parasitol Res 1993;79:73-4. google scholar
  • 21. Jones, WR. The experimental infection of rats with Entamoeba histolytica; with a method for evaluating the anti-amoebic properties of new compounds. Annals of Tropical Medicine and Parasitology 1946; 40: 130-40. google scholar
  • 22. Grecu (Mătiut) DS, Neagu AN, Hărmănescu EA, Moglan I, In vitro division modalities developed by Blastocystis hominis examined with the acridine orange stain. Analele Stiintifice ale Universitătii Alexandru Ioan Cuza” din Iaşi, s. Biologie animală, Tom LIX 2013;13-8 google scholar
  • 23. Stensvold CR. Comparison of sequencing (barcode region) and sequence-tagged-site PCR for Blastocystis subtyping. J. Clin. Microbiol 2013, 51: 190–4. google scholar
  • 24. Böhm-Gloning B, Knobloch J, Walderich B. Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR-amplified 16S-like rDNA. Trop Med In Health 1997;2 (8):771–8. google scholar
  • 25. Santín M, Gómez-Muñoz MT, Solano-Aguilar G, Fayer R. Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals. Parasitol Res. 2011;109(1):205-12. google scholar
  • 26. Roberts T, Barrat J, Harkness J, Ellis J, Stark D. Comparison of microscopy, culture and conventional polymerase chain reaction for detection of Blastocystis sp. in clinical stool samples. Am J Trop Med Hy.g 2011; 84:308-12. google scholar
  • 27. Zaman V, Khan KZ. A comparison of direct microscopy with culture for the diagnosis of Blastocysis hominis.Southeast Asian J Trop Med Public Health 1994; 25:792-3. google scholar
  • 28. Kukoschke KG, Necker A, Muller HE. Detection of Blastocystis hominis by direct microscopy and culture. Eur J Clin Microbiol Infec Dis 1990; 9:305-7. google scholar
  • 29. Boeck, W. C., and J. Drbohlav. The cultivation of Endamoeba histolytica. Am. J. Hyg 1925; 5:371-407. google scholar
  • 30. Leelayoova S, Taamasri P, Rangsin R, Naaglor T, Thathaisong U, Mungthin M. In-vitro cultivation: a sensitive method for detecting Blastocystis hominis. Ann Trop Med Parasitol 2002; 96:803–7. google scholar
  • 31. Stensvold CR, Arendrup MC, Jespersgaard C, Molbak K, Nielsen HV. Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study. Diagn Microbiol Infect Dis 2007;59:303–7. google scholar
  • 32. Dogruman-Al F, Turk S, Adiyaman-Korkmaz G, Hananel A, Levi L, Kopelowitz J,et al. A novel ELISA test for laboratory diagnosis of Blastocystis spp. in human stool spesimens. Parasitol Res 2015; 114:495-500. google scholar
  • 33. Termmathurapoj S , Leelayoova S , Aimpun P , Thathaisong U ,Nimmanon T , Taamasri P , Mungthin M. The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens . Parasitol Res 2004; 93: 445 – 7 . google scholar
  • 34. Hanauer SB. Inflammatory bowel disease: epidemiology, pathogenesis, and therapeutic opportunities. Inflamm Bowel Dis 2006;1:3-9. google scholar
  • 35. Cenac N, Andrews CN, Holzhausen M, Chapman K, Cottrell G, Andrade-Gordon P, Steinhoff M, Barbara G, Beck P, Bunnet NW, Sharkey KA, Ferraz JG, Shaffer E, Vergnolle N. Role for protease activity in visceral pain in irritable bowel syndrome. J Clin Invest 2007; 117: 636-47. google scholar
  • 36. Gecse K, Roka R, Ferrier R, Leveque M, Eutamene H, Cartier C, Ait-Belgnaoui A, Rosztoczy A, Izbeki F, Fiaramonti J, Wittmann T, Bueno L. Increased feacal serine protease activity in diarrhoeic IBS patients: a colonic luminal factor impairing colonic permeability and sensitivity 2008;57:591-9. google scholar
  • 37. Puthia M., Vaithilingam A., Lu J., Tan K. Degradation of human secretory immunoglobulin a by Blastocystis. Parasitol Res 2005;97:386-9. google scholar
  • 38. Mahmoud MS, Saleh WA. Secretory and humoral antibody responses to Blastocystis hominis in symptomatic and asymptomatic human infections. J Egypt Soc Parasitol. 2003; 33:13-30. google scholar
  • 39. Markell EK, Udkow MP. Blastocystis hominis: pathogen or fellow traveler?. Am Trop Med Hyg 1986; 35:1023-26. google scholar
  • 40. Petersen AM, Stensvold CR, Mirsepasi H, Engberg J, Friis-Møller A, Porsbo LJ, Hammerum AM, Nordgaard-Lassen I, Nielsen HV, Krogfelt KA. Active ulcerative colitis associated with low prevalence of Blastocystis and Dientamoeba fragilis infection. Scand J Gastroenterol 2013; 48:638-9. google scholar
  • 41. Rossen NG, Bart A, Verhaar N, van Nood E, Kootte R, de Groot PF, D'Haens GR, Ponsioen CY, van Gool T. Low prevalence of Blastocystis sp. in active ulcerative colitis patients. Eur J Clin Microbiol Infect Dis 2015; 34:1039-44. 42. Cekin AH, Cekin Y, Adakan Y, Tasdemir E, Koclar FG, Yolcular BO. Blastocystosis in patients with gastrointestinal symptoms: a case–control study. BMC Gastroenterol 2012; 12:122. google scholar
  • 43. Yamamoto-Furusho JK, Torijano-Carrera E. Intestinal protozoa infections among patients with ulcerative colitis: prevalence and impact on clinical disease course. Digestion 2010;82:18–23. google scholar
  • 44. Tai WP, Hu PJ, Wu J, Lin XC: Six ulcerative colitis patients with refractory symptoms co-infective with Blastocystis hominis in China. Parasitol Res 2011;108:1207–10. google scholar
  • 45. Dogruman-Al F, Kustimur S, Yoshikawa H, Tuncer C, Simsek Z, Tanyuksel M, et al. Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey. Mem Inst Oswaldo Cruz, Rio de Janeiro 2009;104:724-7. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Büyükbaba Boral, Ö., Çelik Payçu, D.G., İliaz, R., Akgül, A., İşsever, H., & Akyüz, F. (2017). EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES. Journal of Istanbul Faculty of Medicine, 80(1), 38-44. https://doi.org/10.18017/iuitfd.315519


AMA

Büyükbaba Boral Ö, Çelik Payçu D G, İliaz R, Akgül A, İşsever H, Akyüz F. EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES. Journal of Istanbul Faculty of Medicine. 2017;80(1):38-44. https://doi.org/10.18017/iuitfd.315519


ABNT

Büyükbaba Boral, Ö.; Çelik Payçu, D.G.; İliaz, R.; Akgül, A.; İşsever, H.; Akyüz, F. EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES. Journal of Istanbul Faculty of Medicine, [Publisher Location], v. 80, n. 1, p. 38-44, 2017.


Chicago: Author-Date Style

Büyükbaba Boral, Özden, and Deniz Gözde Çelik Payçu and Raim İliaz and Akın Akgül and Halim İşsever and Filiz Akyüz. 2017. “EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES.” Journal of Istanbul Faculty of Medicine 80, no. 1: 38-44. https://doi.org/10.18017/iuitfd.315519


Chicago: Humanities Style

Büyükbaba Boral, Özden, and Deniz Gözde Çelik Payçu and Raim İliaz and Akın Akgül and Halim İşsever and Filiz Akyüz. EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES.” Journal of Istanbul Faculty of Medicine 80, no. 1 (Sep. 2025): 38-44. https://doi.org/10.18017/iuitfd.315519


Harvard: Australian Style

Büyükbaba Boral, Ö & Çelik Payçu, DG & İliaz, R & Akgül, A & İşsever, H & Akyüz, F 2017, 'EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES', Journal of Istanbul Faculty of Medicine, vol. 80, no. 1, pp. 38-44, viewed 6 Sep. 2025, https://doi.org/10.18017/iuitfd.315519


Harvard: Author-Date Style

Büyükbaba Boral, Ö. and Çelik Payçu, D.G. and İliaz, R. and Akgül, A. and İşsever, H. and Akyüz, F. (2017) ‘EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES’, Journal of Istanbul Faculty of Medicine, 80(1), pp. 38-44. https://doi.org/10.18017/iuitfd.315519 (6 Sep. 2025).


MLA

Büyükbaba Boral, Özden, and Deniz Gözde Çelik Payçu and Raim İliaz and Akın Akgül and Halim İşsever and Filiz Akyüz. EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES.” Journal of Istanbul Faculty of Medicine, vol. 80, no. 1, 2017, pp. 38-44. [Database Container], https://doi.org/10.18017/iuitfd.315519


Vancouver

Büyükbaba Boral Ö, Çelik Payçu DG, İliaz R, Akgül A, İşsever H, Akyüz F. EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES. Journal of Istanbul Faculty of Medicine [Internet]. 6 Sep. 2025 [cited 6 Sep. 2025];80(1):38-44. Available from: https://doi.org/10.18017/iuitfd.315519 doi: 10.18017/iuitfd.315519


ISNAD

Büyükbaba Boral, Özden - Çelik Payçu, DenizGözde - İliaz, Raim - Akgül, Akın - İşsever, Halim - Akyüz, Filiz. EVALUATION OF BLASTOCYSTIS SPP. AMONG INFLAMMATORY BOWEL DISEASE PATIENTS WITH DIFFERENT DIAGNOSTIC METHODS AND SUBTYPE ANALYSES”. Journal of Istanbul Faculty of Medicine 80/1 (Sep. 2025): 38-44. https://doi.org/10.18017/iuitfd.315519



TIMELINE


Submitted30.10.2016
Accepted16.03.2017
Published Online31.03.2017

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE



Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.