Journal of Istanbul Faculty of Medicine

SUBMITINFORMATION
EnglishTürkçe

Research Article


DOI :10.26650/IUITFD.2019.0023   IUP :10.26650/IUITFD.2019.0023    Full Text (PDF)

THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION

Sacide Pehlivan, Savaş Gürsoy, Ayşe Feyda Nursal, Mazlum Serdar Akaltun, Kürşat Özdilli, Mustafa Pehlivan

Objective: Ankylosing spondylitis (AS) is a chronic inflammatory disorder. MicroRNAs (miRNAs) can function as either oncogenes or tumor suppressor genes. Altered miRNA expression has been implicated in the pathogenesis of several diseases. Therefore, we aimed to explore the effects of miR-196a2T/C (rs11614913) variant profile on susceptibility to AS in a Turkish population.

Materials and Methods: Blood samples were collected from 78 AS patients and 79 healthy controls. miR-196a2T/C variant was genotyped by PCR-RFLP. Odds ratio (OR) with 95% confidence interval (95%CI) were calculated using the χ2 test.

Results: The frequency of T/C and T/T genotypes of the miR196a2T/C were higher in AS patients compared to healthy controls (p=0.034 and p=0.028, respectively). The subjects carrying the miR-196a2T/C variant T/T genotype showed a 2.542-fold increased AS risk than the control group. However, no difference was observed in the allele frequencies of miR-196a2T/C between AS patients and the controls. It was found that C/C genotype of miR-196a2T/C variant was more frequent in AS patients with enthesitis than AS patients without enthesitis (p=0.042).

Conclusion: The miR-196a2T/C variant represents a genetic risk factor for increased susceptibility to AS and is associated with enthesitis in the Turkish population.

Keywords: Ankylosing spondylitis, miR-196a2, variant, enthesis
DOI :10.26650/IUITFD.2019.0023   IUP :10.26650/IUITFD.2019.0023    Full Text (PDF)

TÜRK POPÜLASYONUNDA ANKİLOZAN SPONDİLİTE YATKINLIK SAĞLAYICI FAKTÖR OLARAK miR-196a2T/C VARYANTI

Sacide Pehlivan, Savaş Gürsoy, Ayşe Feyda Nursal, Mazlum Serdar Akaltun, Kürşat Özdilli, Mustafa Pehlivan

Amaç: Ankilozan spondilit (AS) kronik, enflamatuar bir hastalıktır. MikroRNA’lar (miRNA’,lar) onkogen veya tümör baskılayıcı genler olarak fonksiyon yapabilirler. Çeşitli hastalıkların patogenezinde miRNA ekspresyonu’nun değişmesi bulunmaktadır. Bu nedenle, Türk toplumunda AS yatkınlığında miR-196a2T/C (rs11614913) varyant profilinin etkilerini araştırmayı amaçladık.

Gereç ve Yöntem: 78 AS hastası ve 79 kontrolden kan örnekleri toplandı. miR-196a2T/C varyantı PCR-RFLP ile genotiplendi. χ2 testi kullanılarak %95 güven aralığı ile Odds oranı (OR) hesaplandı.

Bulgular: miR-196a2T/C T/C ve T/T genotipleri AS hastalarında sağlıklı kontrollere kıyasla daha yüksekti (sırasıyla, p=0.034 and p=0.028). miR-196a2T/C varyant T/T genotipini taşıyan kişiler kontrol grubundan 2,542 kat artmış AS riski gösterdiler. Ancak, miR-196a2T/C allele frekansları açısından AS hastaları ve kontroller arasında fark saptanmadı. miR-196a2T/C varyant C/C genotipinin entezitli AS hastalarında entezit bulunmayan AS hastalarından daha çok olduğu bulundu (p=0.042).

Sonuç: miR-196a2T/C varyantı Türk toplumunda AS ve hastalığın entezit ile ilişkisine yatkınlık sağlayan genetik risk faktörü rolü göstermektedir.


Keywords: Ankilozan spondilit, miR-196a2, varyant, entezit

PDF View

References

  • 1. Han R, Xia Q, Xu S, Fan D, Pan F. Interleukin-23 receptor polymorphism (rs10889677 A/C) in ankylosing spondylitis: Meta-analysis in Caucasian and Asian populations. Clin Chim Acta 2018;477:53-9. google scholar
  • 2. Wang Y, Yi XD, Lu HL. Influence of CYP2C9 and COX2 Genetic Polymorphisms on Clinical Efficacy of NonSteroidal Anti-Inflammatory Drugs in Treatment of Ankylosing Spondylitis. Med Sci Monit 2017;23:1775-82. google scholar
  • 3. Niu Z, Wang J, Zou H, Yang C, Huang W, Jin L. Common MIR146A Polymorphisms in Chinese Ankylosing Spondylitis Subjects and Controls. PLoS One 2015;10:e0137770. google scholar
  • 4. Filipowicz W, Bhattacharyya SN, Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat Rev Genet 2008;9:102-14. google scholar
  • 5. Lu J, Getz G, Miska EA, Alvarez-Salveda E, Lamb J, Peck D, et al. MicroRNA expression profiles classify human cancers. Nature 2005;435:834-38. [CrossRef] google scholar
  • 6. O’Connell RM, Rao DS, Chaudhuri AA, Baltimore D. Physiological and pathological roles for microRNAs in the immune system. Nat Rev Immunol 2010;10:111-22. google scholar
  • 7. Hornstein E, Mansfield JH, Yekta S, Hu JK, Harfe BD, McManus MT, et al. The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development. Nature 2005;438:67174. google scholar
  • 8. Lim JJ, Shin DA, Jeon YJ, Kumar H, Sohn S, Min HS, et al. Association of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ossification of the Posterior Longitudinal Ligament of the Cervical Spine. PLoS One 2016;11:e0159756. google scholar
  • 9. van der Linden S, Valkenburg HA, Cats A. Evaluation of diagnostic criteria for ankylosing spondylitis. A proposal for modification of the New York criteria. Arthritis Rheum 1984;27:361-8. google scholar
  • 10. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16:1215. google scholar
  • 11. Bodal VK, Sangwan S, Bal MS, Kaur M, Sharma S, Kaur B. Association between Microrna 146a and Microrna 196a2 Genes Polymorphism and Breast Cancer Risk in North Indian Women. Asian Pac J Cancer Prev 2017;18:2345-8. google scholar
  • 12. Smith JA. Update on ankylosing spondylitis: current concepts in pathogenesis.Curr Allergy Asthma Rep 2015;15:489. google scholar
  • 13. Xiao C, Rajewsky K. MicroRNA control in the immune system: basic principles. Cell 2009;136:26-36. google scholar
  • 14. Hackl M, Heilmeier U, Weilner S, Grillari J. Circulating microRNAs as novel biomarkers for bone diseases - Complex signatures for multifactorial diseases? Mol Cell Endocrinol 2016;432:83-95. google scholar
  • 15. Arfat Y, Xiao WZ, Ahmad M, Zhao F, Li DJ, Sun YL, et al. Role of microRNAs in osteoblasts differentiation and bone disorders. Curr Med Chem 2015;22:748-58. [CrossRef] 16. Xu HY, Wang ZY, Chen JF, Wang TY, Wang LL, Tang LL, et al. Association between Ankylosing Spondylitis and the miR-146a and miR-499 Polymorphisms. PLoS One 2015;10:e0122055. google scholar
  • 17. Yang L, Du L, Yue Y, Huang Y, Zhou Q, Cao S, et al. miRNA Copy Number Variants Confer Susceptibility to Acute Anterior Uveitis With or Without Ankylosing Spondylitis. Invest Ophthalmol Vis Sci 2017;58:1991-2001. google scholar
  • 18. Qian BP, Ji ML, Qiu Y, Wang B, Yu Y, Shi W, et al. Identification of Serum miR-146a and miR-155 as Novel Noninvasive Complementary Biomarkers for Ankylosing Spondylitis. Spine (Phila Pa 1976) 2016;41:735-42. google scholar
  • 19. Duan S, Mi S, Zhang W, Dolan ME. Comprehensive analysis of the impact of SNPs and CNVs on human microRNAs and their regulatory genes. RNA Biol 2009;6:412-25. google scholar
  • 20. Lu J, Clark AG. Impact of microRNA regulation on variation in human gene expression. Genome Res 2012;22:1243-54. [CrossRef] google scholar
  • 21. Park R, Lee WJ, Ji JD. Association between the three functional miR-146a single-nucleotide polymorphisms, rs2910164, rs57095329, and rs2431697, and autoimmune disease susceptibility: A meta-analysis. Autoimmunity 2016;49:451-8. google scholar
  • 22. Fattah SA, Ghattas MH, Saleh SM, Abo-Elmatty DM. Premicro RNA-499 Gene Polymorphism rs3746444 T/C is Associated with Susceptibility to Rheumatoid Arthritis in Egyptian Population.Indian J Clin Biochem 2018;33:96-101. google scholar
  • 23. Hu Z, Chen J, Tian T, Zhou X, Gu H, Xu L, et al. Genetic variants of miRNA sequences and non-small cell lung cancer survival. J Clin Invest 2008;118:2600-8. [CrossRef] 24. Vinci S, Gelmini S, Pratesi N, Conti S, Malentacchi F, Simi L, et al. Genetic variants in miR-146a, miR-149, miR-196a2, miR-499 and their influence on relative expression in lung cancers. Clin Chem Lab Med 2011;49:2073-80. google scholar
  • 25. Ahn DH, Rah H, Choi YK, Jeon YJ, Min KT, Kwack K, et al. Association of the miR-146aC>G, miR-196a2C>T, and miR499A>G polymorphisms with gastric cancer and survival in the Korean population. Mol Carcinog 2013;52(S1):E39-E51. google scholar
  • 26. Jeon YJ, Kim OJ, Kim SY, Oh SH, Oh D, Kim OJ, et al. Association of the miR-146a, miR-149, miR-196a2, and miR499 polymorphisms with ischemic stroke and silent brain infarction risk. Arterioscler Thromb Vasc Biol 2013;33:42030. google scholar
  • 27. Jeon YJ, Kim SY, Rah H, Choi DH, Cha SH, Yoon TK, et al. Association of the miR-146aC>G, miR-149T>C, miR196a2T>C, and miR-499A>G polymorphisms with risk of spontaneously aborted fetuses. Am J Reprod Immunol 2012;68:408-17. google scholar
  • 28. Qi J, Hou S, Zhang Q, Liao D, Wei L, Fang J, et al. A functional variant of pre-miRNA-196a2 confers risk for Behcet’s disease but not for Vogt-Koyanagi-Harada syndrome or AAU in ankylosing spondylitis. Hum Genet 2013;132:1395-404. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Pehlivan, S., Gürsoy, S., Nursal, A., Akaltun, M., Özdilli, K., & Pehlivan, M. (2020). THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. Journal of Istanbul Faculty of Medicine, 83(2), 81-85. https://doi.org/10.26650/IUITFD.2019.0023


AMA

Pehlivan S, Gürsoy S, Nursal A, Akaltun M, Özdilli K, Pehlivan M. THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. Journal of Istanbul Faculty of Medicine. 2020;83(2):81-85. https://doi.org/10.26650/IUITFD.2019.0023


ABNT

Pehlivan, S.; Gürsoy, S.; Nursal, A.; Akaltun, M.; Özdilli, K.; Pehlivan, M. THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. Journal of Istanbul Faculty of Medicine, [Publisher Location], v. 83, n. 2, p. 81-85, 2020.


Chicago: Author-Date Style

Pehlivan, Sacide, and Savaş Gürsoy and Ayşe Feyda Nursal and Mazlum Serdar Akaltun and Kürşat Özdilli and Mustafa Pehlivan. 2020. “THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION.” Journal of Istanbul Faculty of Medicine 83, no. 2: 81-85. https://doi.org/10.26650/IUITFD.2019.0023


Chicago: Humanities Style

Pehlivan, Sacide, and Savaş Gürsoy and Ayşe Feyda Nursal and Mazlum Serdar Akaltun and Kürşat Özdilli and Mustafa Pehlivan. “THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION.” Journal of Istanbul Faculty of Medicine 83, no. 2 (Nov. 2024): 81-85. https://doi.org/10.26650/IUITFD.2019.0023


Harvard: Australian Style

Pehlivan, S & Gürsoy, S & Nursal, A & Akaltun, M & Özdilli, K & Pehlivan, M 2020, 'THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION', Journal of Istanbul Faculty of Medicine, vol. 83, no. 2, pp. 81-85, viewed 24 Nov. 2024, https://doi.org/10.26650/IUITFD.2019.0023


Harvard: Author-Date Style

Pehlivan, S. and Gürsoy, S. and Nursal, A. and Akaltun, M. and Özdilli, K. and Pehlivan, M. (2020) ‘THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION’, Journal of Istanbul Faculty of Medicine, 83(2), pp. 81-85. https://doi.org/10.26650/IUITFD.2019.0023 (24 Nov. 2024).


MLA

Pehlivan, Sacide, and Savaş Gürsoy and Ayşe Feyda Nursal and Mazlum Serdar Akaltun and Kürşat Özdilli and Mustafa Pehlivan. “THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION.” Journal of Istanbul Faculty of Medicine, vol. 83, no. 2, 2020, pp. 81-85. [Database Container], https://doi.org/10.26650/IUITFD.2019.0023


Vancouver

Pehlivan S, Gürsoy S, Nursal A, Akaltun M, Özdilli K, Pehlivan M. THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. Journal of Istanbul Faculty of Medicine [Internet]. 24 Nov. 2024 [cited 24 Nov. 2024];83(2):81-85. Available from: https://doi.org/10.26650/IUITFD.2019.0023 doi: 10.26650/IUITFD.2019.0023


ISNAD

Pehlivan, Sacide - Gürsoy, Savaş - Nursal, Ayşe Feyda - Akaltun, Mazlum Serdar - Özdilli, Kürşat - Pehlivan, Mustafa. “THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION”. Journal of Istanbul Faculty of Medicine 83/2 (Nov. 2024): 81-85. https://doi.org/10.26650/IUITFD.2019.0023



TIMELINE


Submitted12.03.2019
Accepted22.08.2019
Published Online24.03.2020

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.