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DOI :10.26650/IUITFD.2021.849531   IUP :10.26650/IUITFD.2021.849531    Full Text (PDF)

VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS

İlknur Bingül, Canan Küçükgergin, Abdurrahman Fatih Aydın, Asiye Işın Doğan Ekici, Semra Doğru Abbasoğlu, Mehmet Müjdat Uysal

Objective: Vitamin D has antioxidant, anti-inflammatory and antiglycation activities, and hepatoprotective potential. There is a relationship between vitamin D deficiency (VDD) and the severity of liver disorders. VDD has been proposed to contribute to the progression of nonalcoholic fatty liver disease (NAFLD). However, experimental results are not clear. Therefore, in this study, the effects of a VDD diet on high fructose (HFr) drinking-induced NAFLD was evaluated. Material and Method: Male Wistar rats were divided into four groups as control, HFr, VDD+HFr, and VDD. Control and HFr groups were fed a control diet, and other groups with a VDD-diet for 12 weeks. HFr (30%; w/v; in drinking water) was given in the last 8 weeks. Insulin resistance (IR), serum lipids, hepatic triglyceride, lipid peroxide, protein carbonyl, advanced glycation end products (AGEs) and inflammation (TNF-α and myeloperoxidase) parameters, and histopathological changes were investigated. Results: Increases in serum transaminases, hypertriglyceridemia, and IR were observed in HFr and VDD+HFr groups. Increased liver triglyceride, lipid and protein oxidation products, protein glycation and inflammation markers as well as microvesicular hepatic steatosis and hepatocyte ballooning were observed in both groups. Although IR and hepatic inflammation markers were higher in the VDD+HFr group, serum transaminases, hepatic triglyceride, lipid and protein oxidation products, and glycation indicators in the liver did not alter between the two groups. However, Nrf2 mRNA expression and superoxide dismutase and glutathione peroxidase mRNA expression and activities were significantly higher in the VDD+HFr group. Conclusion: Our results show that VDD did not augmented HFr-induced hepatotoxicity and glycooxidative stress in the liver of rats.

Keywords: Vitamin D deficiency, high fructose diet, nonalcoholic liver disease, glycooxidative stress, antioxidant, inflammation
DOI :10.26650/IUITFD.2021.849531   IUP :10.26650/IUITFD.2021.849531    Full Text (PDF)

VİTAMİN D EKSİKLİĞİ SIÇANLARDA ALKOLE BAĞLI OLMAYAN YAĞLI KARACİĞER HASTALIĞININ PROGRESYONUNU ARTTIRMADI

İlknur Bingül, Canan Küçükgergin, Abdurrahman Fatih Aydın, Asiye Işın Doğan Ekici, Semra Doğru Abbasoğlu, Mehmet Müjdat Uysal

Amaç: Vitamin D antioksidan, antiinflamatuvar ve antiglikasyon etkinliğe ve karaciğeri koruyucu potansiyele sahiptir. Vitamin D eksikliği/yetersizliği (VDD/VDI) ile karaciğer bozukluklarının ciddiyeti arasında bir ilişki bulunmaktadır. VDD’nin alkole bağlı olmayan yağlı karaciğer hastalığının (NAFLD) progresyonunda etkili olduğu bildirilmiştir. Fakat deneysel sonuçlar yeterli değildir. Bu nedenle, bu çalışmada VDD’nin yüksek fruktozlu (HFr) içme suyu uygulanarak oluşturulan NAFLD üzerine etkisi incelendi. Gereç ve Yöntemler: Erkek Wistar sıçanlar kontrol, HFr, VDD+Fr ve VDD olmak üzere 4 gruba ayrıldı. Kontrol ve HFr grupları Vit D3 içeren, diğerleri ise Vit D3 içermeyen yemle 12 hafta beslendiler. HFr (%30; w/v) içme suyu ile son 8 hafta uygulandı. İnsulin direnci (IR), serum lipitleri, hepatik trigliserit, lipit peroksit, protein karbonil, ileri glikasyon ürünleri (AGEs) ve inflamasyon (TNF-α ve miyeloperoksidaz) göstergeleri tayin edildi. Bulgular: HFR ve VDD+HFr gruplarında serumda transaminazlar arttı, hipertrigliseridemi, ve insülin direnci oluştu. Her iki grupta da karaciğerde trigliserit düzeyleri, lipit ve protein oksidasyon ürünleri, protein glikasyon ve inflamasyon göstergeleri arttı, mikroveziküler steatoz ve hepatosit balonlaşması saptandı. IR ve inflamasyon göstergeleri VDD+HFr grubunda daha yüksek olmasına rağmen, iki grup arasında serum transaminazları, karaciğer trigliserit, lipit ve protein oksidasyon ürünleri ve glikasyon göstergeleri düzeylerinde bir farklılık bulunmadı. Ancak Nrf2 mRNA ekspresyonu ile süperoksit dismutaz ve glutatyon peroksidazın mRNA ekspresyonları ve aktivitelerinin VDD+HFr grubunda daha yüksek olduğu bulundu. Sonuç: Sonuçlarımız VDD’nin HFr ile oluşturulan karaciğer hasarı ve glikooksidatif streste bir değişiklik oluşturmadığını göstermektedir.

Keywords: Vitamin D yetersizliği, yüksek fruktozlu diyet, alkolik olmayan karaciğer hastalığı, glikooksidatif stres, anti oksidanlar, inflamasyon

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APA

Bingül, İ., Küçükgergin, C., Aydın, A.F., Doğan Ekici, A.I., Doğru Abbasoğlu, S., & Uysal, M.M. (2021). VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS. Journal of Istanbul Faculty of Medicine, 84(3), 360-368. https://doi.org/10.26650/IUITFD.2021.849531


AMA

Bingül İ, Küçükgergin C, Aydın A F, Doğan Ekici A I, Doğru Abbasoğlu S, Uysal M M. VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS. Journal of Istanbul Faculty of Medicine. 2021;84(3):360-368. https://doi.org/10.26650/IUITFD.2021.849531


ABNT

Bingül, İ.; Küçükgergin, C.; Aydın, A.F.; Doğan Ekici, A.I.; Doğru Abbasoğlu, S.; Uysal, M.M. VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS. Journal of Istanbul Faculty of Medicine, [Publisher Location], v. 84, n. 3, p. 360-368, 2021.


Chicago: Author-Date Style

Bingül, İlknur, and Canan Küçükgergin and Abdurrahman Fatih Aydın and Asiye Işın Doğan Ekici and Semra Doğru Abbasoğlu and Mehmet Müjdat Uysal. 2021. “VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS.” Journal of Istanbul Faculty of Medicine 84, no. 3: 360-368. https://doi.org/10.26650/IUITFD.2021.849531


Chicago: Humanities Style

Bingül, İlknur, and Canan Küçükgergin and Abdurrahman Fatih Aydın and Asiye Işın Doğan Ekici and Semra Doğru Abbasoğlu and Mehmet Müjdat Uysal. “VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS.” Journal of Istanbul Faculty of Medicine 84, no. 3 (Dec. 2024): 360-368. https://doi.org/10.26650/IUITFD.2021.849531


Harvard: Australian Style

Bingül, İ & Küçükgergin, C & Aydın, AF & Doğan Ekici, AI & Doğru Abbasoğlu, S & Uysal, MM 2021, 'VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS', Journal of Istanbul Faculty of Medicine, vol. 84, no. 3, pp. 360-368, viewed 23 Dec. 2024, https://doi.org/10.26650/IUITFD.2021.849531


Harvard: Author-Date Style

Bingül, İ. and Küçükgergin, C. and Aydın, A.F. and Doğan Ekici, A.I. and Doğru Abbasoğlu, S. and Uysal, M.M. (2021) ‘VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS’, Journal of Istanbul Faculty of Medicine, 84(3), pp. 360-368. https://doi.org/10.26650/IUITFD.2021.849531 (23 Dec. 2024).


MLA

Bingül, İlknur, and Canan Küçükgergin and Abdurrahman Fatih Aydın and Asiye Işın Doğan Ekici and Semra Doğru Abbasoğlu and Mehmet Müjdat Uysal. “VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS.” Journal of Istanbul Faculty of Medicine, vol. 84, no. 3, 2021, pp. 360-368. [Database Container], https://doi.org/10.26650/IUITFD.2021.849531


Vancouver

Bingül İ, Küçükgergin C, Aydın AF, Doğan Ekici AI, Doğru Abbasoğlu S, Uysal MM. VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS. Journal of Istanbul Faculty of Medicine [Internet]. 23 Dec. 2024 [cited 23 Dec. 2024];84(3):360-368. Available from: https://doi.org/10.26650/IUITFD.2021.849531 doi: 10.26650/IUITFD.2021.849531


ISNAD

Bingül, İlknur - Küçükgergin, Canan - Aydın, AbdurrahmanFatih - Doğan Ekici, AsiyeIşın - Doğru Abbasoğlu, Semra - Uysal, MehmetMüjdat. “VITAMIN D DEFICIENCY DID NOT AUGMENT THE PROGRESSION OF HIGH-FRUCTOSE-INDUCED NONALCOHOLIC FATTY LIVER DISEASE IN RATS”. Journal of Istanbul Faculty of Medicine 84/3 (Dec. 2024): 360-368. https://doi.org/10.26650/IUITFD.2021.849531



TIMELINE


Submitted29.12.2020
Accepted16.04.2021
Published Online14.07.2021

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