Orijinal Araştırma


DOI :10.17096/jiufd.40536   IUP :10.17096/jiufd.40536    Tam Metin (PDF)

TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU

Burcu ÖzdemirBülent KurtişGülay TüterBurcu ŞengüvenBenay YıldırımGönen Özcan

Amaç: Trombositten-zengin plazma (TZP) otojen büyüme faktörlerini yara bölgesine taşıyan bir yöntemdir. Bu araştırmada çift TZP uygulamasının (Ç-TZP) tavşan kraniyal defektlerinde kemik iyileşmesi üzerine etkilerini osteonektin (ON) ve osteokalsin (OC) ekspresyonlarının değerlendirilerek incelenmesi amaçlandı.

Gereç ve Yöntem: Her birinde cerrahi olarak oluşturulmuş iki adet kraniyal defekt olan yirmi sekiz tavşan çalışmaya dâhil edildi. Defektler (n=56) tek TZP uygulamasıyla (T-TZP)(n=10), tek TZP uygulaması ve betatrikalsiyumfosfat kombinasyonuyla (T-TZP+β-TCP)(n=10), Ç-TZP ile (n=8) veya Ç-TZP ve β-TCP kombinasyonuyla (Ç-TZP+ β-TCP) (n=8) tedavi edilenler ve control grubu (n=10) olmak üzere altı gruba ayrıldı. Deney hayvanları 30. Günde sakrifiye edildiler ve örnekler immunohistokimyasal olarak ON ve OC için incelendi.

Bulgular: Ç-TZP uygulamasının T-TZP ya da kontrol gruplarında elde edilen ON ve OC yüzdelerini anlamlı olarak arttırmadığı görüldü. β-TCP uygulanan üç grupta, β-TCP uygulanmayan gruplara göre daha yüksek ON yüzdeleri saptandı (p<0.05). β-TCP uygulanan gruplar ile T-TZP uygulanan grupta Ç-TZP ve kontrol grubuna göre daha yüksek OC yüzdeleri izlendi (p<0.05).

Sonuç: Mevcut çalışmamızın sonuçları Ç-TZP’nin kritik boyutlu olmayan tavşan kraniyal defektlerinde iyileşme üzerine anlamlı etkileri olmadığını göstermiştir.

DOI :10.17096/jiufd.40536   IUP :10.17096/jiufd.40536    Tam Metin (PDF)

OSTEOCALCIN AND OSTEONECTIN EXPRESSION AFTER DOUBLEAPPLICATION OF PLATELET-RICH PLASMA IN RABBITS

Burcu ÖzdemirBülent KurtişGülay TüterBurcu ŞengüvenBenay YıldırımGönen Özcan

Purpose: Platelet-rich plasma (PRP) is a novel method for transferring autogenous growth factors to the wound area. The aim of this study was to evaluate the efficacy of double-application of PRP (DA-PRP) on bone healing in rabbit cranial defects by examining osteonectin (ON) and osteocalcin (OC) expression.

Materials and Methods: Twenty-eight rabbits, each with two surgically prepared calvarial bone defects, were included in this study and divided into six groups: The defects (N=56) were treated with either a single-application of PRP (SA-PRP) (n=10), a combination of SA-PRP and betatricalciumphosphate (SA-PRP+β-TCP) (n=10), only DAPRP (n=8), both DA-PRP and beta-tricalciumphosphate (DA-PRP+β-TCP) (n=8), only beta-tricalciumphosphate (β-TCP) (n=10), or controls (n=10). The animals were sacrificed at 30th day postoperatively and samples were immunohistochemically examined for ON and OC expressions.

Results: It was determined that DA-PRP did not significantly improve the ON and OC percentages achieved by SA-PRP or the controls. The three groups treated with β-TCP showed a higher percentage of ON than those treated without β-TCP (p<0.05). The β-TCP treated groups and SA-PRP group demonstrated higher OC percentage than DA-PRP and control groups (p<0.05).

Conclusion: The present findings suggest that DAPRP did not have a significant effect on the healing of non-critical size rabbit cranial bone defects.


PDF Görünüm

Referanslar

  • Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85(6):638-646. google scholar
  • Tsay RC, Vo J, Burke A, Eisig SB, Lu HH, Landesberg R. Differential growth factor retention by platelet-rich plasma composites. J Oral Maxillofac Surg 2005;63(4):521-528. google scholar
  • Kovacs K, Velich N, Huszar T, Szabo G, Semjen G, Reiczigel J, Suba Z. Comparative study of beta-tricalcium phosphate mixed with platelet-rich plasma versus beta-tricalcium phosphate, a bone substitute material in dentistry. Acta Vet Hung 2003;51(4):475-484. google scholar
  • Monov G, Fuerst G, Tepper G, Watzak G, Zechner W, Watzek G. The effect of platelet-rich plasma upon implant stability measured by resonance frequency analysis in the lower anterior mandibles. Clin Oral Implants Res 2005;16(4):461-465. google scholar
  • Lekovic V, Camargo PM, Weinlaender M, Vasilic N, Kenney EB. Comparison of platelet-rich plasma, bovine porous bone mineral, and guided tissue regeneration versus platelet-rich plasma and bovine porous bone mineral in the treatment of intrabony defects: A reentry study. J Periodontol 2002;73(2):198-205. google scholar
  • Anitua E. Plasma rich in growth factors: Preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14(4):529-535. google scholar
  • Yazawa M, Ogata H, Kimura A, Nakajima T, Mori T, Watanabe N. Basic studies on the bone formation ability by platelet rich plasma in rabbits. J Craniofac Surg 2004;15(3):439-446. google scholar
  • Kovacs K, Velich N, Huszar T, Fenyves B, Suba Z, Szabo G. Histomorphometric and densitometric evaluation of the effects of platelet-rich plasma on the remodeling of beta-tricalcium phosphate in beagle dogs. J Craniofac Surg 2005;16(1):150-154. google scholar
  • Raghoebar GM, Schortinghuis J, Liem RS, Ruben JL, van der Wal JE, Vissink A. Does platelet-rich plasma promote remodeling of autologous bone grafts used for augmentation of the maxillary sinus floor? Clin Oral Implants Res 2005;16(3):349-356. google scholar
  • Thorwarth M, Rupprecht S, Falk S, Felszeghy E, Wiltfang J, Schlegel KA. Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)-an immunohistochemical analysis. Biomaterials 2005;26(15):2575-2584. google scholar
  • Crovetti G, Martinelli G, Issi M, Barone M, Guizzardi M, Campanati B, Moroni M, Carabelli A. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci 2004;30(2):145-151. google scholar
  • Driver VR, Hanft J, Fylling CP, Beriou JM, Autologel Diabetic Foot Ulcer Study G. A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. Ostomy Wound Manage 2006;52(6):68-70, 72, 74 passim. google scholar
  • Redaelli A, Romano D, Marciano A. Face and neck revitalization with platelet-rich plasma (prp): Clinical outcome in a series of 23 consecutively treated patients. J Drugs Dermatol 9(5):466-472. google scholar
  • Kon E, Buda R, Filardo G, Di Martino A, Timoncini A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M. Platelet-rich plasma: Intra-articular knee injections produced favorable results on degenerative cartilage lesions. Knee Surg Sports Traumatol Arthrosc 18(4):472-479. google scholar
  • Ozdemir B, Kurtis B, Tuter G, Senguven B, Tokman B, Pinar-Ozdemir S, Demirel I, Ozcan G. Double-application of platelet-rich plasma on bone healing in rabbits. Med Oral Patol Oral Cir Bucal 17(1):e171-177. google scholar
  • Aghaloo TL, Moy PK, Freymiller EG. Investigation of platelet-rich plasma in rabbit cranial defects: A pilot study. J Oral Maxillofac Surg 2002;60(10):1176-1181. google scholar
  • Merten HA, Wiltfang J, Grohmann U, Hoenig JF. Intraindividual comparative animal study of alpha- and beta-tricalcium phosphate degradation in conjunction with simultaneous insertion of dental implants. J Craniofac Surg 2001;12(1):59-68. google scholar
  • Christenson RH. Biochemical markers of bone metabolism: An overview. Clin Biochem 1997;30(8):573-593. google scholar
  • Heinegard D, Oldberg A. Structure and biology of cartilage and bone matrix noncollagenous macromolecules. FASEB J 1989;3(9):2042-2051. google scholar
  • Termine JD, Kleinman HK, Whitson SW, Conn KM, McGarvey ML, Martin GR. Osteonectin, a bone-specific protein linking mineral to collagen. Cell 1981;26(1 Pt 1):99-105. google scholar
  • Swaminathan R. Biochemical markers of bone turnover. Clin Chim Acta 2001;313(1-2):95-105. google scholar
  • Lieberman JR, Daluiski A, Einhorn TA. The role of growth factors in the repair of bone. Biology and clinical applications. J Bone Joint Surg Am 2002;84-A(6):1032-1044. google scholar
  • Fisher LW, Termine JD. Noncollagenous proteins influencing the local mechanisms of calcification. Clin Orthop Relat Res 1985, 200):362-385. google scholar
  • Otsuka K, Yao KL, Wasi S, Tung PS, Aubin JE, Sodek J, Termine JD. Biosynthesis of osteonectin by fetal porcine calvarial cells in vitro. J Biol Chem 1984;259(15):9805-9812. google scholar
  • Kroese-Deutman HC, Wolke JG, Spauwen PH, Jansen JA. Closing capacity of cranial bone defects using porous calcium phosphate cement implants in a rabbit animal model. J Biomed Mater Res A 2006;79(3):503-511. google scholar
  • Zhu JX, Sasano Y, Takahashi I, Mizoguchi I, Kagayama M. Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. Histochem J 2001;33(1):25-35. google scholar
  • Clarke B. Normal bone anatomy and physiology. Clin J Am Soc Nephrol 2008;3 Suppl 3(S131-139. google scholar
  • Cowles EA, DeRome ME, Pastizzo G, Brailey LL, Gronowicz GA. Mineralization and the expression of matrix proteins during in vivo bone development. Calcif Tissue Int 1998;62(1):74-82. google scholar
  • Wiesmann HP, Meyer U, Plate U, Hohling HJ. Aspects of collagen mineralization in hard tissue formation. Int Rev Cytol 2005;242:121-156. google scholar
  • Boskey AL, Gadaleta S, Gundberg C, Doty SB, Ducy P, Karsenty G. Fourier transform infrared microspectroscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin. Bone 1998;23(3):187-196. google scholar
  • Hunter GK, Hauschka PV, Poole AR, Rosenberg LC, Goldberg HA. Nucleation and inhibition of hydroxyapatite formation by mineralized tissue proteins. Biochem J 1996;317(1):59-64. google scholar
  • Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS. Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 1990;143(3):420-430. google scholar
  • Velich N, Nemeth Z, Hrabak K, Suba Z, Szabo G. Repair of bony defect with combination biomaterials. J Craniofac Surg 2004;15(1):11-15. google scholar
  • Wiltfang J, Kloss FR, Kessler P, Nkenke E, Schultze-Mosgau S, Zimmermann R, Schlegel KA. Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment. Clin Oral Implants Res 2004;15(2):187-193. google scholar
  • Harnack L, Boedeker RH, Kurtulus I, Boehm S, Gonzales J, Meyle J. Use of platelet-rich plasma in periodontal surgery--a prospective randomised double blind clinical trial. Clin Oral Investig 2009;13(2):179-187. google scholar
  • Ozdemir B, Okte E. Treatment of intrabony defects with beta-tricalciumphosphate alone and in combination with platelet-rich plasma. J Biomed Mater Res B Appl Biomater 2012;100(4):976-983. google scholar
  • Kondo N, Ogose A, Tokunaga K, Ito T, Arai K, Kudo N, Inoue H, Irie H, Endo N. Bone formation and resorption of highly purified beta-tricalcium phosphate in the rat femoral condyle. Biomaterials 2005;26(28):5600-5608. google scholar

Atıflar

Biçimlendirilmiş bir atıfı kopyalayıp yapıştırın veya seçtiğiniz biçimde dışa aktarmak için seçeneklerden birini kullanın


DIŞA AKTAR



APA

Özdemir, B., Kurtiş, B., Tüter, G., Şengüven, B., Yıldırım, B., & Özcan, G. (2016). TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU. European Oral Research, 50(2), 1-9. https://doi.org/10.17096/jiufd.40536


AMA

Özdemir B, Kurtiş B, Tüter G, Şengüven B, Yıldırım B, Özcan G. TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU. European Oral Research. 2016;50(2):1-9. https://doi.org/10.17096/jiufd.40536


ABNT

Özdemir, B.; Kurtiş, B.; Tüter, G.; Şengüven, B.; Yıldırım, B.; Özcan, G. TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU. European Oral Research, [Publisher Location], v. 50, n. 2, p. 1-9, 2016.


Chicago: Author-Date Style

Özdemir, Burcu, and Bülent Kurtiş and Gülay Tüter and Burcu Şengüven and Benay Yıldırım and Gönen Özcan. 2016. “TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU.” European Oral Research 50, no. 2: 1-9. https://doi.org/10.17096/jiufd.40536


Chicago: Humanities Style

Özdemir, Burcu, and Bülent Kurtiş and Gülay Tüter and Burcu Şengüven and Benay Yıldırım and Gönen Özcan. TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU.” European Oral Research 50, no. 2 (Sep. 2024): 1-9. https://doi.org/10.17096/jiufd.40536


Harvard: Australian Style

Özdemir, B & Kurtiş, B & Tüter, G & Şengüven, B & Yıldırım, B & Özcan, G 2016, 'TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU', European Oral Research, vol. 50, no. 2, pp. 1-9, viewed 19 Sep. 2024, https://doi.org/10.17096/jiufd.40536


Harvard: Author-Date Style

Özdemir, B. and Kurtiş, B. and Tüter, G. and Şengüven, B. and Yıldırım, B. and Özcan, G. (2016) ‘TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU’, European Oral Research, 50(2), pp. 1-9. https://doi.org/10.17096/jiufd.40536 (19 Sep. 2024).


MLA

Özdemir, Burcu, and Bülent Kurtiş and Gülay Tüter and Burcu Şengüven and Benay Yıldırım and Gönen Özcan. TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU.” European Oral Research, vol. 50, no. 2, 2016, pp. 1-9. [Database Container], https://doi.org/10.17096/jiufd.40536


Vancouver

Özdemir B, Kurtiş B, Tüter G, Şengüven B, Yıldırım B, Özcan G. TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU. European Oral Research [Internet]. 19 Sep. 2024 [cited 19 Sep. 2024];50(2):1-9. Available from: https://doi.org/10.17096/jiufd.40536 doi: 10.17096/jiufd.40536


ISNAD

Özdemir, Burcu - Kurtiş, Bülent - Tüter, Gülay - Şengüven, Burcu - Yıldırım, Benay - Özcan, Gönen. TAVŞANLARDA ÇİFT TROMBOSİTTEN ZENGİN PLAZMA UYGULAMASI SONRASINDA OSTEOKALSİN VE OSTEONEKTİN EKSPRESYONU”. European Oral Research 50/2 (Sep. 2024): 1-9. https://doi.org/10.17096/jiufd.40536



ZAMAN ÇİZELGESİ


Gönderim20.10.2015
Kabul21.01.2016
Çevrimiçi Yayınlanma01.04.2016

PAYLAŞ




İstanbul Üniversitesi Yayınları, uluslararası yayıncılık standartları ve etiğine uygun olarak, yüksek kalitede bilimsel dergi ve kitapların yayınlanmasıyla giderek artan bilimsel bilginin yayılmasına katkıda bulunmayı amaçlamaktadır. İstanbul Üniversitesi Yayınları açık erişimli, ticari olmayan, bilimsel yayıncılığı takip etmektedir.