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DOI :10.26650/experimed.1489790   IUP :10.26650/experimed.1489790    Tam Metin (PDF)

Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression

Sevgin DeğirmencioğluPınar ÇetinalpMuhammed SeyithanoğluSevda Tanrıkulu KüçükHikmet KoçakYıldız Öner İyidoğan

Objective: Adipose tissue stores lipids necessary for the maintenance of nutritional homeostasis. It is also an endocrine organ that reacts to changes in inflammation and energy status. Capsaicin, the principal bioactive compound in red pepper, has garnered significant attention for its reported anti-obesity, anti-diabetic, anti-oxidant, and anti-inflammatory properties. In this study, we aimed to elucidate the influence and most efficacious dose of capsaicin on the expression of lipid metabolism-related inflammatory proteins and the inhibition of adipocyte cell differentiation.

Materials and Methods: Cell viability analysis was performed using CCK-8, cell differentiation was assessed using Oil Red O, and gene expression levels of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), adiponectin, leptin, cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), nuclear factor kappa B1 (NF-κB1), tumor necrosis factor-alpha (TNF-α), sirtuin-1 (SIRT-1), transient receptor potential vanilloid receptor 1 (TRPV1), and uncoupling protein 2 (UCP2) were evaluated using quantitative real time polymerase chain reaction (qRT-PCR). Statistical analyses were conducted using GraphPad Prism 5. One-way ANOVA was performed to compare quantitative data between the groups.

Results: Capsaicin suppressed preadipocyte-to-adipocyte differentiation and mitigated the release of pro-inflammatory cytokines, particularly at low concentrations. Capsaicin effectively suppressed adiponectin levels at all concentrations but decreased leptin levels at lower concentrations (0.5 µM and 1 µM). Capsaicin stimulated the expressions of SIRT1 and TRPV-1 in adipocytes. According to our findings, the most effective capsaicin dose for the regulation of SIRT1 and TRPV-1 expressions appears to be 20 μM.

Conclusion: Capsaicin’s effect on proteins regulating adipogenesis is not dose-related, but its inhibitory effect on adiposity-dependent inflammation was more pronounced at low concentrations.


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APA

Değirmencioğlu, S., Çetinalp, P., Seyithanoğlu, M., Tanrıkulu Küçük, S., Koçak, H., & Öner İyidoğan, Y. (2024). Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression. Experimed, 14(2), 116-125. https://doi.org/10.26650/experimed.1489790


AMA

Değirmencioğlu S, Çetinalp P, Seyithanoğlu M, Tanrıkulu Küçük S, Koçak H, Öner İyidoğan Y. Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression. Experimed. 2024;14(2):116-125. https://doi.org/10.26650/experimed.1489790


ABNT

Değirmencioğlu, S.; Çetinalp, P.; Seyithanoğlu, M.; Tanrıkulu Küçük, S.; Koçak, H.; Öner İyidoğan, Y. Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression. Experimed, [Publisher Location], v. 14, n. 2, p. 116-125, 2024.


Chicago: Author-Date Style

Değirmencioğlu, Sevgin, and Pınar Çetinalp and Muhammed Seyithanoğlu and Sevda Tanrıkulu Küçük and Hikmet Koçak and Yıldız Öner İyidoğan. 2024. “Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression.” Experimed 14, no. 2: 116-125. https://doi.org/10.26650/experimed.1489790


Chicago: Humanities Style

Değirmencioğlu, Sevgin, and Pınar Çetinalp and Muhammed Seyithanoğlu and Sevda Tanrıkulu Küçük and Hikmet Koçak and Yıldız Öner İyidoğan. Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression.” Experimed 14, no. 2 (Nov. 2024): 116-125. https://doi.org/10.26650/experimed.1489790


Harvard: Australian Style

Değirmencioğlu, S & Çetinalp, P & Seyithanoğlu, M & Tanrıkulu Küçük, S & Koçak, H & Öner İyidoğan, Y 2024, 'Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression', Experimed, vol. 14, no. 2, pp. 116-125, viewed 16 Nov. 2024, https://doi.org/10.26650/experimed.1489790


Harvard: Author-Date Style

Değirmencioğlu, S. and Çetinalp, P. and Seyithanoğlu, M. and Tanrıkulu Küçük, S. and Koçak, H. and Öner İyidoğan, Y. (2024) ‘Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression’, Experimed, 14(2), pp. 116-125. https://doi.org/10.26650/experimed.1489790 (16 Nov. 2024).


MLA

Değirmencioğlu, Sevgin, and Pınar Çetinalp and Muhammed Seyithanoğlu and Sevda Tanrıkulu Küçük and Hikmet Koçak and Yıldız Öner İyidoğan. Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression.” Experimed, vol. 14, no. 2, 2024, pp. 116-125. [Database Container], https://doi.org/10.26650/experimed.1489790


Vancouver

Değirmencioğlu S, Çetinalp P, Seyithanoğlu M, Tanrıkulu Küçük S, Koçak H, Öner İyidoğan Y. Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression. Experimed [Internet]. 16 Nov. 2024 [cited 16 Nov. 2024];14(2):116-125. Available from: https://doi.org/10.26650/experimed.1489790 doi: 10.26650/experimed.1489790


ISNAD

Değirmencioğlu, Sevgin - Çetinalp, Pınar - Seyithanoğlu, Muhammed - Tanrıkulu Küçük, Sevda - Koçak, Hikmet - Öner İyidoğan, Yıldız. Capsaicin Modulates Adipocyte Cell Differentiation and Inflammatory Gene Expression”. Experimed 14/2 (Nov. 2024): 116-125. https://doi.org/10.26650/experimed.1489790



ZAMAN ÇİZELGESİ


Gönderim25.05.2024
Kabul02.08.2024
Çevrimiçi Yayınlanma26.08.2024

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