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DOI :10.26650/experimed.2020.0007   IUP :10.26650/experimed.2020.0007    Tam Metin (PDF)

Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi

Arif Çolak, Ayşen Yarat, Turay Yardımcı

Diabetik komplikasyonların gelişmesinde, hiperglisemi kaynaklı oksidatif stres çok önemli bir rol oynamaktadır. Döteryum azlığının hücresel hasara karşı ve tümör baskılayıcı etki üzerine koruyucu etkisi olduğu kanser hücre hatlarında gösterilmiştir. Çalışmanın amacı, döteryum azaltılmasının sıçanlarda streptozotosin (STZ) ile oluşturulmuş deneysel diabette eritrosit oksidan ve antioksidan sistem parametreleri üzerindeki etkisinin araştırılması ve değerlendirilmesidir. STZ enjeksiyonunu takiben 30. veya 40. günde, kan örnekleri alınıp, kan glukoz değerleri ölçülmüş, eritrosit hemolizatları da hazırlanmıştır. Eritrositlerin lipid peroksidasyon ve glutatyon seviyeleri ile glutatyon-S-transferaz, süperoksit dismutaz ve katalaz enzim aktiviteleri tayin edilmiştir. 30 veya 40 günlük periyotlardaki döteryumu azaltılmış su tüketiminin, eritrosit oksidan-antioksidan parametre düzeylerine etkisinin farklı olduğu görülmüştür. İçme suyunda döteryumun 150 ppm den 85 ppm’e azaltılmasının net etkisi glutatyon düzeylerinde gözlenmiş olup hem kontrol hem de diyabetik gruplarda eritrosit glutatyon düzeylerini artırdığı saptanmıştır. Sonuç olarak, döteryumu azaltılmış suyun, diabete bağlı oksidatif hasara karşı koruyucu etkisi olabilir. Döteryumu azaltılmış suyun etki mekanizmasının, uygun dozunun ve uygulama süresinin aydınlatılması için daha fazla çalışmalara ihtiyaç vardır.

Anahtar Kelimeler: Deneysel diabet, eritrosit, döteryum azlığı, antioksidan ve oksidan parametreler
DOI :10.26650/experimed.2020.0007   IUP :10.26650/experimed.2020.0007    Tam Metin (PDF)

Effect of Deuterium Depletion on Erythrocytes in Experimental Diabetes

Arif Çolak, Ayşen Yarat, Turay Yardımcı

In the development of diabetic complications, oxidative stress caused by hyperglycemia plays an important role. Deuterium depletion has protective effect against cellular damage and tumor suppressor effect in cancer cell lines. The purpose of this study was to examine and evaluate the effect of deuterium depletion on erythrocyte oxidant and antioxidant system parameters in experimental diabetes induced by streptozotocin (STZ) in rats. On the 30th or 40th day after STZ injection, blood samples were taken and blood glucose levels were measured. Erythrocyte hemolysates were also prepared. Lipid peroxidation and glutathione levels and glutathione-S-transferase, superoxide dismutase and catalase enzyme activities of erythrocytes were determined. At the 30 or 40 day periods, deuterium depleted water (DDW) consumption had differently affected oxidant-antioxidant parameters of erythrocytes. The clear effect of deuterium depletion in drinking water from 150 ppm to 85 ppm was observed in glutathione levels and it increased erythrocyte glutathione levels in both the control and diabetic groups. In conclusion, deuterium depletion may have protective effects on diabetes induced oxidative stress. Further studies are needed to be done to determine the mechanism of action and the useful dosage and the application period of DDW. 

Anahtar Kelimeler: Experimental diabetes, erythrocyte, deuterium depletion, antioxidant and oxidant parameters

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Referanslar

  • 1. Collins CJ, Bowman NS (Eds). Effects In Chemical Reactions. Van Nostrand Reinhold, York, pp. 286-363, 1971. google scholar
  • 2. Wiberg KB. The Deuterium Isotope Effect. Chem Rev. 1955, 55: 713-43. [CrossRef] google scholar
  • 3. Jancso G, Van Hook WA. Condensed Phase Isotope Effects: Especially Vapor Pressure. Isotope Effects 1974; 74: 689-750. [CrossRef] google scholar
  • 4. Rundel PW, Ehleringer JR, Nagy KA: Stable In Ecological Research. Springer, York, pp. 7-9, 1988. [CrossRef] google scholar
  • 5. Blaga L. The Deuterium content of human metabolic fluids in relation with human metabolic processes (in Romania), IFA-Bucuresti, Scientific Report, 1978, 1-4. google scholar
  • 6. Berdea P, Cuna S, Cazacu M, Tudose M. Deuterium Variation of Human Blood Serum. Studia Universitatis Babeş-Bolyai, Physica, Special Issue 2001; 256-8. google scholar
  • 7. Somlyai G, Jancso G, Jakli G, Vass K, Barna B, Lakics V, Gaal T. Naturally occuring deuterium is essential for the normal growth rate of cells. FEBS Letters 1993; 317: 1-4. [CrossRef] google scholar
  • 8. Somlyai G. Defeating Cancer. The Biological Effect of Deuterium Depletion by Gabor Somlyai HYD Ltd. for Research and Development ISBN: 0-7596-9261-0;2002. google scholar
  • 9. Somlyai G, Laskay G, Berkenyi T, Galbacs Z, Galbacks G, Kiss SA, Jakli GY, Jancso G. The Biological Effects of Deuterium-Depleted Water, a Possible New Tool in Cancer Therapy. Journal of Oncology 1998; 30(4): 91-4.135. google scholar
  • 10. Cong F, Zhang Y, Sheng H, Ao Z, Zhang S, Wang J. Deuterium-depleted water inhibits human lung carcinoma cell growth by apoptosis. Experimental and Therapeutic Medicine 2010; 1: 277-83. [CrossRef] google scholar
  • 11. Somlyai G, Molnar M, Laskay G, Szabo M, Berkenyi T, Guller I, Kovaks A. Biological significance of naturally occurring deuterium: the antitumor effect of deuterium depletion. Orvosi Hetilap 2010; 5; 151(36): 1455-60. [CrossRef] google scholar
  • 12. Keempels K, Somlyi I, Somlyai G. A retrospective evaluation of the effects of deuterium depleted water consumption on 4 patients with brain metastases from lung cancer. Integrative Cancer Therapies 2008; 7(3): 172-81. [CrossRef] google scholar
  • 13. Katz JJ, Crespi HL, Hasterlik RJ, Thomson JF, Finkel AJ. Some Observations on Biological Effects of Deuterium, with Special Reference to Effects on Neoplastic Processes. Journal of the National Cancer Institute 1957; 18(5): 641-58. google scholar
  • 14. Synergistic Effects of Deuterium Oxide and Gemcitabine in Human Pancreatic Cancer Cell Line, Cancer Letter 2008; 259: 231-9. [CrossRef] google scholar
  • 15. Bahk JY, Lee JH, Chung HS, LeeHY, Chung BC, Park MS, Min SK, Kim MO. Anticancer Effect of Deuterium Oxide on a Bladder Cancer Cell Related to Bcl-2 and Bax. Journal of Indian Engineering Chemistry 2007; 13(4): 501-7. google scholar
  • 16. MaritimAC, Sanders RA, Watkins JB. Diabetes, Oxidative Stress and Antioxidants: A Review. Journal of Biochemical and Molecular Toxicology 2003; 17(1): 24-38. [CrossRef] google scholar
  • 17. Nwose EU, Jelinek HF, Richards RS, Kerr PG. Erythrocyte oxidative stress in clinical management of diabetes and its cardiovascular complications. British Journal of Biomedical Sciences 2007; 64(1): 35-43. [CrossRef] google scholar
  • 18. Shin S, Ku Y, Babu N, Singh M. Erythrocyte deformability and its variation in diabetes mellitus. Indian Journal of Experimental Biology 2007; 45(1): 121-8. google scholar
  • 19. Leoncini S, Rossi V, Signorini C, Tanganelli I, Comporti M, Ciccoli L. Oxidative stress, erythrocyte ageing and plasma non-proteinbound iron in diabetic patients. Free Radical Research 2008; 42(8): 716-4. [CrossRef] google scholar
  • 20. Alekseevich TA et al. Method for treatment of patients with diabetes mellitus, 01-10(ru2270017), 2006. google scholar
  • 21. Tatsuo U, Kunihiro S. Treatment of Cancer, Diabetes Mellitus, Leukemia, Aplastic Anemia and Insomnia, Treatment and Prevention of Severe Acute Respıratory Syndrome (Sars), Rejuvenation, Activation of Cell and Promotion of Increase of Immune Cell by Soy Sauce Prepared by Using Deuterium-Attrited Water (Super Light Water) as Raw Material, and Method for Producing The Same. Internatl Scient KK. A23L1/238; 12-24 (JP2004357678), 2004. google scholar
  • 22. Habig WH, Jacoby WB. Assays for differentiation of glutathione-s- transferases. Methods in Enzymology 1981; 77: 398-405. [CrossRef] google scholar
  • 23. Aebi H.: Catalase invitro. In: Methods of enzymatic Analysis, 2nd Edition, vol.2, Bergmeyer HU (Ed), FL., pp.121-126. google scholar
  • 24. Mylorie AA, Collins H, Umbles C, Kyle J. Erythrocyte superoxide dismutase activity and other parameters of cupper status in rats ingesting lead acetate. Toxicology and Applied Pharmacology 1986; 82: 512-520. [CrossRef] google scholar
  • 25. Ledwozyw A, Michalak D, Stepien A, Kadziolka A. The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis. Clinica Chimica Acta 1986; 155; 275-83. [CrossRef] google scholar
  • 26. Beutler E.: Glutathione in Red Cell Metabolism: A manual of Biochemical methods, 2nd ed.: Grune and Stratton, NY., pp.112-114. google scholar
  • 27. Watkins PJ. ABC of Diabetes. 5th ed, BMJ Publishing Group Ltd, London, p.2-3,2003. google scholar
  • 28. Pietropaolo M, Trucco M.Genetics of Type I Diabetes. In: Type 1 Diabetes. Ed: Sperling MA, Humana Press Inc, New Jersey, p.28, 2003. google scholar
  • 29. Yarat A, Yanardag R, Tunalı T, Sacan O, Gursoy F, Emekli N, Ustuner A, Ergenekon G. Effects of glibornuride versus metformin on eye lenses and skin in experimental diabetes. Arzneimittel Forschung Drug Research 2006; 56(7): 541-6. [CrossRef] google scholar
  • 30. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiological Research 2001; 50: 53646. google scholar
  • 31. Junod A, Lambert AE, Stauffacher W, Renold AE. Diabetogenic action of streptozotocin: Relation of dose to metabolic response. The Journal of Clinical Investigation 1969; 48: 2129-39. [CrossRef] google scholar
  • 32. Tatsuki R, Satoh K, Yamamoto A, Hoshi K, Ichihara K. Lipid peroxidation in the pancreas and other organs in streptozotocin diabetic rats. Japanese Journal Pharmacology 1997; 75: 267-73. [CrossRef] google scholar
  • 33. Coşkun O, Ocakcı A, Bayraktaroğlu T, Kanter M. Exercise training prevents and protects streptozotocin-induced oxidative stress and β- cell damage in rat pancreas. Tohoku Journal Experimental Medicine 2004; 203: 145-54. [CrossRef] google scholar
  • 34. Atkinson MA, Eisenbarth GS. Type 1 diabetes: new perspectives on disease pathogenesis and treatment. Lancet 2001; 358: 221-9. [CrossRef] google scholar
  • 35. Griesmacher A, Kindhauser M, Andert SE, Schreiner W, Tomo C, Kroebl P, Pietschmann P, Prager R, Schnack C, Schernthaner G, Mueller M. Enhanced Serum levels of thiobarbituric acid - reactive substances in diabetes mellitus. American Journal of Medicine 1995; 98(5): 469-75. [CrossRef] google scholar
  • 36. Giugliano D, Ceriello A, Paolisso G. Oxidative stress and diabetic vascular complications. Diabetes Care 1996; 19: 257-67. [CrossRef] google scholar
  • 37. Qujeq D, Rezvani T. Catalase (antioxidant enzyme) activity in streptozotocin-induced diabetic rats. International Journal of Diabetes and Metabolism 2007; 15: 22-4. google scholar
  • 38. Sekar N, Kanthasamy A, William S, Balasubramaniyan N, Govindasamy S. Antioxidant effect of vanadate on experimental diabetic rats. Acta Diabetologica Latina 1990; 27: 285-93. [CrossRef] google scholar
  • 39. Mukherjee B, Mukherjee JR, Chatterjee M. Lipid peroxidation, glutathione levels and changes in glutathione-related enzyme activities in streptozotocin-induced diabetic rats. Immunology and Cell Biology 1994; 72: 109-14. [CrossRef] google scholar
  • 40. Ramakrishna V, Jailkhani R. Evalution of oxidative stress in insulin dependent diabetes mellitus (IDDM) patients. Diagnostic Pathology 2007; 2: 22. [CrossRef] google scholar
  • 41. Peuchant E, Brun JL, Rigalleau V, Dubourg L, Thomas MJ, Daniel JY, Leng JJ, Gin H. Oxidative and antioxidative status in pregnant women with either gestational or type 1 diabetes. Clinical Biochemistry 2004; 37: 293-8. [CrossRef] google scholar
  • 42. Bhatia S, Shukla R, Madhu SR, Gambhir JK, Prabhu KM. Antioxidant status, lipid peroxidation and nitric oxide end product in patients of type 2 diabetes mellitus with nephropathy. Clinical Biochemistry 2003; 36: 557-62. [CrossRef] google scholar
  • 43. Memişoğulları R, Taysı S, Bakan E, Capoğlu I. Antioxidant status and lipid peroxidation in type II diabetes mellitus. Cellular Biochemical Function 2003; 21: 291-6. [CrossRef] google scholar
  • 44. Sailaja YR, Baskar R, Saralakumari D. Antioxidant status during maturation of reticulocytes to erythrocytes in type 2 diabetics. Free Radical Biology and Medicine 2003; 35(2): 133-9. [CrossRef] google scholar
  • 45. Vural H, Sabuncu T, Arslan SO, Aksoy N. Melatonin inhibits lipid peroxidation and stimulates the antioxidant status of diabetic rats. Journal of Pineal Research 2001; 31: 193-8. [CrossRef] google scholar
  • 46. Chandramohan G, Al-Numair KS, Pugalendi KV. Restoration of altered plasma, erythrocyte and liver antioxidant levels. International Journal of Integrative Biology 2009; 5(3): 176-81. google scholar
  • 47. Bild W, Nastasa V, Haulica I. In vivo and in vitro research on biological effects of deuterium-depleted water: 1. Influence of deuterium-deoleted water on cultured cell growth. Romanian Journal of Physiology 2004; 41(1-2): 53-67. google scholar
  • 48. Haulica I, Peculea M, Stefanescu I, Titescu G, Todiras M, Bild W. Effects of heavy and deuterium-depleted water on vascular reactivity. Romanian Journal of Physiology 1998; 35(1-2): 25-32. google scholar
  • 49. Bild W, Stefanescu I, Haulica I, Lupusoru C, Titescu G, Iliescu R, Nastas V. Research concerning the radioprotective and immunostimulating effects of deuterium depleted water. Romanian Journal of Physiology 1999; 36(3-4): 205-18. google scholar
  • 50. Loike JD, Caol L, Kuang K, Vera JC, Silverstein SC, Fischbarg J. Role of facilitative glucose transporters in diffusional water permeability through J774 cells. Journal of Genetic Physiology 1993; 102(5): 897-906. [CrossRef] google scholar
  • 51. Kovács A, Guller I, Krempels K, Somlyai I, Jánosi I, Gyomgyi Z, Szabó I, Ember IGS (2011) Deuterium depletion may delay the progression of prostate cancer. J Cancer Ther 2011; 2: 548-56. [CrossRef] google scholar
  • 52. Ávila DS, Somlyai G, Somlyai I, Aschner M. Anti-aging effects of deuterium depletion on Mn-induced toxicity in a C. Elegans Model. Toxicol Lett 2012; 211: 319-24. [CrossRef] google scholar
  • 53. Gyöngyi Z, Budan F, Szabo I, Ember I, Kiss I, Krempels K, Somlyai I, Somlyai G. Deuterium depleted water effects on survival of lung cancer patients and expression of Kras, Bcl2, and Myc genes in Mouse lung. Nutr Cancer 2013; 65(2): 240-6. [CrossRef] google scholar
  • 54. Mladin C, Ciobica A, Lefter R, Popescu A, Bild W. Deuterium depleted water has stimulating effects on long-term memory in rats. Neurosci Lett 2014; 583: 154-8. [CrossRef] google scholar
  • 55. Soleyman-Jahi S, Zendehdel K, Akbarzahed K, Haddadi M, Amanpour S, Muhammadnejad S. In vitro Assessment of antineoplastik effects of döteryum depleted water. Asian Pac J Cancer Prev 2014; 15(5): 2179-83. [CrossRef] google scholar
  • 56. Lisitsyn AB, Baryshev MG, Basov AA, Barysheva EV, Bykov IM, DyDykin AS, Tekutskaia EE, Timakov AA, Fedulova LV, Chernukha IM, Dzhimak SS. Influence of deuterium depleted water on the organism of laboratory animals in various functional conditions of nonspecific protective system. Biofizika 2014; 59(4): 757-65. [CrossRef] google scholar
  • 57. Strekalova T, Evans M, Chernopiatko A, Couch Y, Costa-Nunes J, Cespuglio R, Chesson L, Vignisse J, Steinbusch HW, Anthony DC, Pomytkin I, Lecsh KP. Deuterium content of water increases depression susceptibility: The potential role of a serotonin-related mechanism. Behavioural Brain Res 2015; 277: 237-44. [CrossRef] google scholar
  • 58. Boros LG, D'Agostino DP, Katz HE, Roth JP, Meuillet EJ, Somlyai G. Submolecular regulation of cell transformation by deuterium depleting water exchange reactions in the tricarboxylic acid substrate cycle. Med Hypothesis 2016; 87: 69-74. [CrossRef] google scholar
  • 59. Dzhimak SS, Basov AA, Baryshev MG. Content of deuterium in biological fluids and organs: Influence of deuterium depleted water on D/H gradient and the process of adaptation. Doki Biochem Biophys 2015; 465(1): 370-373. [CrossRef] google scholar

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APA

Çolak, A., Yarat, A., & Yardımcı, T. (2020). Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi. Experimed, 10(1), 30-37. https://doi.org/10.26650/experimed.2020.0007


AMA

Çolak A, Yarat A, Yardımcı T. Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi. Experimed. 2020;10(1):30-37. https://doi.org/10.26650/experimed.2020.0007


ABNT

Çolak, A.; Yarat, A.; Yardımcı, T. Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi. Experimed, [Publisher Location], v. 10, n. 1, p. 30-37, 2020.


Chicago: Author-Date Style

Çolak, Arif, and Ayşen Yarat and Turay Yardımcı. 2020. “Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi.” Experimed 10, no. 1: 30-37. https://doi.org/10.26650/experimed.2020.0007


Chicago: Humanities Style

Çolak, Arif, and Ayşen Yarat and Turay Yardımcı. “Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi.” Experimed 10, no. 1 (Dec. 2024): 30-37. https://doi.org/10.26650/experimed.2020.0007


Harvard: Australian Style

Çolak, A & Yarat, A & Yardımcı, T 2020, 'Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi', Experimed, vol. 10, no. 1, pp. 30-37, viewed 21 Dec. 2024, https://doi.org/10.26650/experimed.2020.0007


Harvard: Author-Date Style

Çolak, A. and Yarat, A. and Yardımcı, T. (2020) ‘Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi’, Experimed, 10(1), pp. 30-37. https://doi.org/10.26650/experimed.2020.0007 (21 Dec. 2024).


MLA

Çolak, Arif, and Ayşen Yarat and Turay Yardımcı. “Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi.” Experimed, vol. 10, no. 1, 2020, pp. 30-37. [Database Container], https://doi.org/10.26650/experimed.2020.0007


Vancouver

Çolak A, Yarat A, Yardımcı T. Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi. Experimed [Internet]. 21 Dec. 2024 [cited 21 Dec. 2024];10(1):30-37. Available from: https://doi.org/10.26650/experimed.2020.0007 doi: 10.26650/experimed.2020.0007


ISNAD

Çolak, Arif - Yarat, Ayşen - Yardımcı, Turay. “Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi”. Experimed 10/1 (Dec. 2024): 30-37. https://doi.org/10.26650/experimed.2020.0007



ZAMAN ÇİZELGESİ


Gönderim05.03.2020
Kabul18.03.2020
Çevrimiçi Yayınlanma13.04.2020

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