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DOI :10.26650/experimed.1384602   IUP :10.26650/experimed.1384602    Tam Metin (PDF)

Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl

Ayça Doğan, Gudrun A. Brockmann

Objective: We aimed to investigate the differences in the immune response to body fat content between the genetically mutant obese BFMI860 (BFMI) mouse strain and the lean C57BL/6Ncrl (B6) mouse strain as a control and the effects of obesity on gene expression on inflammation-related pathways in epididymal adipose tissue.

Materials and Methods: Six males from each strain were maintained on a standard maintenance diet (SMD) or a high-fat diet (HFD). At the age of 10 weeks, serum and epididymal adipose tissue samples were collected for cytokine and gene expression analyses. RNA samples from epididymal adipose tissue were hybridized using the microarray technique to study the quantitative transcript amounts of genes.

Results: Pathway analysis of gene expression data revealed no considerable development of inflammatory state in BFMI and B6 on SMD. Both strains responded to HFD distinctly; the inflammatory state was more prominent in the obese BFMI group than in the lean B6 group. Several genes, such as Adipoq, NFkbia, Plaur, F2r, C3ar1, and Nfatc4 in pathways involved in the immune system have been found to be differentially regulated in BFMI mice. Under the condition of obesity in BFMI mice, the induction of inflammation-related pathways indicates an increased risk of insulin resistance, atherosclerosis, and cardiovascular disease.

Conclusion: This study identified distinct expression patterns of genes involved in inflammatory pathways, particularly those associated with the adipocytokine signaling pathway and complement and coagulation cascades, in the epididymal adipose tissue of BFMI and B6 mice. The BFMI strain is a valuable and promising model for clarifying the mechanisms underlying obesity and the activation of inflammation in adipose tissue.

Anahtar Kelimeler: Obesity, high-fat diet, adipocytokine signaling pathway, complement and coagulation cascade

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APA

Doğan, A., & Brockmann, G.A. (2024). Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl. Experimed, 14(2), 73-84. https://doi.org/10.26650/experimed.1384602


AMA

Doğan A, Brockmann G A. Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl. Experimed. 2024;14(2):73-84. https://doi.org/10.26650/experimed.1384602


ABNT

Doğan, A.; Brockmann, G.A. Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl. Experimed, [Publisher Location], v. 14, n. 2, p. 73-84, 2024.


Chicago: Author-Date Style

Doğan, Ayça, and Gudrun A. Brockmann. 2024. “Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl.” Experimed 14, no. 2: 73-84. https://doi.org/10.26650/experimed.1384602


Chicago: Humanities Style

Doğan, Ayça, and Gudrun A. Brockmann. “Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl.” Experimed 14, no. 2 (Dec. 2024): 73-84. https://doi.org/10.26650/experimed.1384602


Harvard: Australian Style

Doğan, A & Brockmann, GA 2024, 'Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl', Experimed, vol. 14, no. 2, pp. 73-84, viewed 22 Dec. 2024, https://doi.org/10.26650/experimed.1384602


Harvard: Author-Date Style

Doğan, A. and Brockmann, G.A. (2024) ‘Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl’, Experimed, 14(2), pp. 73-84. https://doi.org/10.26650/experimed.1384602 (22 Dec. 2024).


MLA

Doğan, Ayça, and Gudrun A. Brockmann. “Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl.” Experimed, vol. 14, no. 2, 2024, pp. 73-84. [Database Container], https://doi.org/10.26650/experimed.1384602


Vancouver

Doğan A, Brockmann GA. Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl. Experimed [Internet]. 22 Dec. 2024 [cited 22 Dec. 2024];14(2):73-84. Available from: https://doi.org/10.26650/experimed.1384602 doi: 10.26650/experimed.1384602


ISNAD

Doğan, Ayça - Brockmann, GudrunA.. “Gene Expression Profile as a Precursor of Inflammation in Mouse Models: BFMI860 and C57BL/6NCrl”. Experimed 14/2 (Dec. 2024): 73-84. https://doi.org/10.26650/experimed.1384602



ZAMAN ÇİZELGESİ


Gönderim01.11.2023
Kabul05.08.2024
Çevrimiçi Yayınlanma26.08.2024

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