Sağlık Bilimlerinde İleri Araştırmalar Dergisi

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DOI :10.26650/JARHS2024-1405790   IUP :10.26650/JARHS2024-1405790    Tam Metin (PDF)

NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ

Onur Patan, Özge Sultan Zengin, Mahmoud Abudayyak

Amaç: Nikel oksit nanopartikülleri (NiO-NP) endüstride geniş bir uygulama alanına sahiptir. Artan kullanım alanları ve miktarları ile birlikte kapsamlı araştırmalar ile araştırmacıların da ilgi odağı olmaktadır. Ancak, kanser hücrelerindeki etkinlikleri ve dolayısıyla yeni kanser ilaçları geliştirme olasılığı hala tartışmalıdır. Çalışmamızda NiO-NP’lerin insan servikal karsinom (HeLa) hücreleri üzerine sitotoksik ve genotoksik etki potansiyeli incelenmiştir.

Gereç ve Yöntem: NiO-NP’lerin sitotoksisitesi 3-(4,5-dimetiltiyazol-2-il)- 2,5-difeniltetrazolyum bromür (MTT), laktat dehidrojenaz (LDH) ve nötral kırmızı alımı (NRU) testleri ile değerlendirilirken genotoksisitesi Comet testi ile araştırılmıştır.

Bulgular: Yarı maksimal inhibitör konsantrasyon (IC50) değerleri MTT testi için 419,6 µg/mL, NRU testi için 316,4 µg/mL ve LDH testi için 119,3 µg/ mL olarak bulunmuştur. Comet testi verilerine göre, NiO-NP’lerine maruz bırakılan hücrelerde DNA hasarı önemli ölçüde indüklenmiştir. Kuyruk yoğunluğunun 120 μg/mL maruziyet için %18,20 düzeylerinde olduğu tespit edilmiştir.

Sonuç: NiO-NP’ler HeLa hücrelerinde sitotoksik ve genotoksik etkili olduğu tespit edilmiştir. Normal bir hücre hattı için tehlikeli gibi görünse de kanser hücresi üzerine bu etkiler yeni bir antikanser ajan olarak NiO-NP’lerin umut verici olabileceğini göstermektedir. Ancak, NiO-NP’lerin kanser tedavisinde bir alternatif olma potansiyelini değerlendirmek üzere diğer kanser hücre hatlarında ve ileri çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler: HeLa hücreleri, Sitotoksisite, Genotoksisite, Nikel oksit nanopartikülü
DOI :10.26650/JARHS2024-1405790   IUP :10.26650/JARHS2024-1405790    Tam Metin (PDF)

CYTOTOXIC AND GENOTOXIC EFFECTS OF NICKEL OXIDE NANOPARTICLES ON HeLa CELLS

Onur Patan, Özge Sultan Zengin, Mahmoud Abudayyak

Objective: Nickel oxide nanoparticles (NiO-NPs) are used invarious applications, and their increasing use has stimulated extensive studies both in vivo and in vitro. Nevertheless, their activity in cancer cells and thus the possibility of developing new anticancer drugs still remain controversial. Therefore, this study was conducted to specifically investigate the cytotoxicity and genotoxicity of NiO-NPs in human cervical carcinoma (HeLa) cells.

Material and Methods: The cytotoxicity of NiO-NPs was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH), and neutral red uptake (NRU) cytotoxicity assays. A comet assay was performed to determine the genotoxicity of NiO-NPs.

Results: The half maximal inhibitory concentration (IC50) values were 419.6, 316.4, and 119.3 µg/mL in the MTT, NRU, and LDH assays, respectively. The comet assay revealed that NiO-NPs caused significant induction of DNA damage in the exposed HeLa cells. The tail intensity was 18.20% at 120 μg/mL.

Conclusion: NiO-NPs were cytotoxic and genotoxic to HeLa cells. Although NiO-NPs may be hazardous for a normal cell line, the effects observed on HeLa cells indicate that NiO-NPs can be proposed as a novel anticancer agent. However, the potential for NiO-NPs in cancer treatment will require additional and comprehensive studies on other cancer cell lines. 

Anahtar Kelimeler: HeLa cells, cytotoxicity, genotoxicity, NiO-NPs

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DIŞA AKTAR



APA

Patan, O., Zengin, Ö.S., & Abudayyak, M. (2024). NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, 7(3), 220-226. https://doi.org/10.26650/JARHS2024-1405790


AMA

Patan O, Zengin Ö S, Abudayyak M. NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ. Sağlık Bilimlerinde İleri Araştırmalar Dergisi. 2024;7(3):220-226. https://doi.org/10.26650/JARHS2024-1405790


ABNT

Patan, O.; Zengin, Ö.S.; Abudayyak, M. NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, [Publisher Location], v. 7, n. 3, p. 220-226, 2024.


Chicago: Author-Date Style

Patan, Onur, and Özge Sultan Zengin and Mahmoud Abudayyak. 2024. “NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi 7, no. 3: 220-226. https://doi.org/10.26650/JARHS2024-1405790


Chicago: Humanities Style

Patan, Onur, and Özge Sultan Zengin and Mahmoud Abudayyak. “NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi 7, no. 3 (Nov. 2024): 220-226. https://doi.org/10.26650/JARHS2024-1405790


Harvard: Australian Style

Patan, O & Zengin, ÖS & Abudayyak, M 2024, 'NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ', Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 7, no. 3, pp. 220-226, viewed 15 Nov. 2024, https://doi.org/10.26650/JARHS2024-1405790


Harvard: Author-Date Style

Patan, O. and Zengin, Ö.S. and Abudayyak, M. (2024) ‘NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ’, Sağlık Bilimlerinde İleri Araştırmalar Dergisi, 7(3), pp. 220-226. https://doi.org/10.26650/JARHS2024-1405790 (15 Nov. 2024).


MLA

Patan, Onur, and Özge Sultan Zengin and Mahmoud Abudayyak. “NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 7, no. 3, 2024, pp. 220-226. [Database Container], https://doi.org/10.26650/JARHS2024-1405790


Vancouver

Patan O, Zengin ÖS, Abudayyak M. NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ. Sağlık Bilimlerinde İleri Araştırmalar Dergisi [Internet]. 15 Nov. 2024 [cited 15 Nov. 2024];7(3):220-226. Available from: https://doi.org/10.26650/JARHS2024-1405790 doi: 10.26650/JARHS2024-1405790


ISNAD

Patan, Onur - Zengin, ÖzgeSultan - Abudayyak, Mahmoud. “NİKEL OKSİT NANOPARTİKÜLLERİNİN HeLa HÜCRELERİNDE SİTOTOKSİK VE GENOTOKSİK ETKİSİ”. Sağlık Bilimlerinde İleri Araştırmalar Dergisi 7/3 (Nov. 2024): 220-226. https://doi.org/10.26650/JARHS2024-1405790



ZAMAN ÇİZELGESİ


Gönderim16.12.2023
Kabul27.02.2024
Çevrimiçi Yayınlanma22.10.2024

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