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DOI :10.26650/JARHS2025-1608689   IUP :10.26650/JARHS2025-1608689    Tam Metin (PDF)

Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line

Ezgi Nurdan Yenilmez TunoğluGamze Nur ÖterRemzi Okan AkarEngin UlukayaSevda Tanrıkulu KüçükFatma Hande Karpuzoğlu

Objective: In the presented research, we aimed to examine the expression of microRNA-128 (miR-128) and long non-coding RNA (lncRNA) the plasmacytoma variant translocation 1 (PVT1) in non-cancerous cells BEAS-2B and human lung carcinoma A549 to identify any potential roles these molecules might play in the cancer development in lung tissue.

Material and Methods: The cell lines used in this research were A549 and BEAS-2B. Total RNA was isolated from these cell lines, and then complementary DNA (cDNA) was synthesised. Using the quantitative real-time PCR (RT-qPCR) method, the expression levels of lncRNA PVT1 and miR-128 were evaluated. The 2-∆∆Ct method was employed to analyse fold changes by normalising to U6 snRNA and GAPDH expressions.

Results: Elevated PVT1 expression was determined in the lung cancer cells (p<0.01). Nevertheless, miR-128 expression did not change statistically significantly (p>0.05). Even though miR-128 is a potential binding target of lncRNA PVT1 according to in silico analysis, the expression profile does not correlate.

Conclusion: Our evaluation of the relationship between lncRNA PVT1 and miR-128 in cancerous cells A549 compared with non-cancerous cells BEAS-2B reveals that PVT1 expression is significantly elevated in lung cancer cells, whereas miR-128 expression remains unchanged. Further investigation is needed to explore whether the interaction between lncRNA PVT1 and miR-128 is indirect. 

Anahtar Kelimeler: Lung cancerA549 cell linePVT1miR-128RT-qPCR
DOI :10.26650/JARHS2025-1608689   IUP :10.26650/JARHS2025-1608689    Tam Metin (PDF)

İnsan Akciğer Karsinomu Hücre Hattında LncRNA PVT1 ve MiR-128'in İfade Düzeylerinin Belirlenmesi

Ezgi Nurdan Yenilmez TunoğluGamze Nur ÖterRemzi Okan AkarEngin UlukayaSevda Tanrıkulu KüçükFatma Hande Karpuzoğlu

Amaç: Araştırmamızın amacı, normal akciğer epitel hücre hatlarında (BEAS-2B) ve insan akciğer karsinomunda (A549), mikroRNA-128 (miR-128) ve uzun kodlamayan RNA (lncRNA) PVT1 ekspresyonunu inceleyerek, bu moleküllerin akciğer kanserinin gelişimi ve ilerlemesinde oynayabileceği potansiyel rolleri belirlemektir.

Gereç ve Yöntemler: Bu araştırmada kullanılan hücre hatları A549 ve BEAS-2B'dir. Gerçek zamanlı kantitatif PCR (RT-qPCR) yöntemi kullanılarak PVT1 ve miR-128'in ifade seviyeleri değerlendirildi. 2-∆∆Ct yöntemi, U6 snRNA ve GAPDH ifadelerine göre normalize ederek kat değişimlerini incelemek için kullanıldı.

Bulgular: Akciğer kanseri hücrelerinde PVT1 ekspresyonunun yükseldiği belirlendi (p<0,01). Bununla birlikte, miR-128 ekspresyonu istatistiksel olarak anlamlı bir şekilde değişmedi (p>0,05). Mir-128, in-silico analizde lncRNA PVT1'in potansiyel bir bağlanma hedefi olmasına rağmen, ekspresyon profili sonuçlarına göre korelasyon görülmedi.

Sonuç: Normal akciğer epitel hücrelerine kıyasla akciğer kanseri hücrelerinde lncRNA PVT1 ve miR-128 arasındaki ilişkinin incelendiği çalışmamız, PVT1 ekspresyonunun akciğer kanseri hücrelerinde önemli ölçüde yüksek olduğunu, buna karşın miR-128 ekspresyonunun değişmeden kaldığını ortaya koymaktadır. LncRNA PVT1 ve miR-128 arasındaki etkileşimin dolaylı olup olmadığını keşfetmek için daha fazla çalışmaya ihtiyaç vardır.


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DIŞA AKTAR



APA

Yenilmez Tunoğlu, E., Öter, G.N., Akar, R.O., Ulukaya, E., Tanrıkulu Küçük, S., & Karpuzoğlu, F.H. (2019). Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, 0(0), -. https://doi.org/10.26650/JARHS2025-1608689


AMA

Yenilmez Tunoğlu E, Öter G N, Akar R O, Ulukaya E, Tanrıkulu Küçük S, Karpuzoğlu F H. Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line. Sağlık Bilimlerinde İleri Araştırmalar Dergisi. 2019;0(0):-. https://doi.org/10.26650/JARHS2025-1608689


ABNT

Yenilmez Tunoğlu, E.; Öter, G.N.; Akar, R.O.; Ulukaya, E.; Tanrıkulu Küçük, S.; Karpuzoğlu, F.H. Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, [Publisher Location], v. 0, n. 0, p. -, 2019.


Chicago: Author-Date Style

Yenilmez Tunoğlu, Ezgi Nurdan, and Gamze Nur Öter and Remzi Okan Akar and Engin Ulukaya and Sevda Tanrıkulu Küçük and Fatma Hande Karpuzoğlu. 2019. “Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi 0, no. 0: -. https://doi.org/10.26650/JARHS2025-1608689


Chicago: Humanities Style

Yenilmez Tunoğlu, Ezgi Nurdan, and Gamze Nur Öter and Remzi Okan Akar and Engin Ulukaya and Sevda Tanrıkulu Küçük and Fatma Hande Karpuzoğlu. Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi 0, no. 0 (Jun. 2025): -. https://doi.org/10.26650/JARHS2025-1608689


Harvard: Australian Style

Yenilmez Tunoğlu, E & Öter, GN & Akar, RO & Ulukaya, E & Tanrıkulu Küçük, S & Karpuzoğlu, FH 2019, 'Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line', Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 0, no. 0, pp. -, viewed 26 Jun. 2025, https://doi.org/10.26650/JARHS2025-1608689


Harvard: Author-Date Style

Yenilmez Tunoğlu, E. and Öter, G.N. and Akar, R.O. and Ulukaya, E. and Tanrıkulu Küçük, S. and Karpuzoğlu, F.H. (2019) ‘Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line’, Sağlık Bilimlerinde İleri Araştırmalar Dergisi, 0(0), pp. -. https://doi.org/10.26650/JARHS2025-1608689 (26 Jun. 2025).


MLA

Yenilmez Tunoğlu, Ezgi Nurdan, and Gamze Nur Öter and Remzi Okan Akar and Engin Ulukaya and Sevda Tanrıkulu Küçük and Fatma Hande Karpuzoğlu. Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 0, no. 0, 2019, pp. -. [Database Container], https://doi.org/10.26650/JARHS2025-1608689


Vancouver

Yenilmez Tunoğlu E, Öter GN, Akar RO, Ulukaya E, Tanrıkulu Küçük S, Karpuzoğlu FH. Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line. Sağlık Bilimlerinde İleri Araştırmalar Dergisi [Internet]. 26 Jun. 2025 [cited 26 Jun. 2025];0(0):-. Available from: https://doi.org/10.26650/JARHS2025-1608689 doi: 10.26650/JARHS2025-1608689


ISNAD

Yenilmez Tunoğlu, Ezgi Nurdan - Öter, GamzeNur - Akar, RemziOkan - Ulukaya, Engin - Tanrıkulu Küçük, Sevda - Karpuzoğlu, FatmaHande. Determining the Expression Levels of LncRNA PVT1 and MiR-128 in the Human Lung Carcinoma Cell Line”. Sağlık Bilimlerinde İleri Araştırmalar Dergisi 0/0 (Jun. 2025): -. https://doi.org/10.26650/JARHS2025-1608689



ZAMAN ÇİZELGESİ


Gönderim29.12.2024
Kabul17.03.2025
Çevrimiçi Yayınlanma09.05.2025

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