Toplantı Özeti


DOI :10.26650/JARHS2021-1142672   IUP :10.26650/JARHS2021-1142672    Tam Metin (PDF)

DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES

Semra DemokanSena ŞenBegüm ÖzemekSevde CömertÖnder EryılmazMurat UlusanNecati EnverUğur SezermanGülsüm AkF. Canan AlatlıMehmet Nejat Dalay

Objectives: DNA methylation, which is the most frequently observed epigenetic change, is very important because it is seen in the early stages of cancer. Abnormal methylation of promoter regions and silencing of tumor suppressor genes play a key role in the development of oral squamous cell carcinoma (OSCC). In our study*, it was aimed to identify and validate new biomarker candidates via epigenomics/transcriptomics approaches, to understand the molecular mechanisms of OSCC and to discover new biomarkers for early diagnosis. Materials and Methods: After DNA/RNA isolation of tumor/matched-normal tissue samples from 6 OSCC patients, BC-DNA/cDNA synthesis was performed, respectively. The methylation and expression profiles were analyzed by the R(v3.5.1) environment methods using IlluminaHumanMethylation450chips and IlluminaiScan, respectively. A candidate gene showing methylation-dependent expression loss after bioinformatic analysis was validated by QRT-PCR AND QMSP methods in tissues and body fluids of 20 OSCC and 20 oral premalignant lesions (OPML) patients, respectively. Results: To identify epigenetic biomarker candidate, we selected gene which show either hypermethylation and lower expression patterns. This candidate gene (unpublished data), which is belong subfamily of the protein-tyrosine kinase, was found to be methylated in 65% of tumors, 20% of matched-normal tissues of OSCC. The methylation rates of the candidate gene in tumor, matched-normal tissues and saliva of OPML patients were found to be 55%, 40% and 10%, respectively. The decreased expression levels of candidate gene were observed in 55% OSCC and 35% OPML tumors, respectively. Conclusions: Our candidate gene could be a biomarker for early detection of OSCC. 


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    APA

    Demokan, S., Şen, S., Özemek, B., Cömert, S., Eryılmaz, Ö., Ulusan, M., Enver, N., Sezerman, U., Ak, G., Alatlı, F.C., & Dalay, M.N. (2022). DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, 5(1), 20-20. https://doi.org/10.26650/JARHS2021-1142672


    AMA

    Demokan S, Şen S, Özemek B, Cömert S, Eryılmaz Ö, Ulusan M, Enver N, Sezerman U, Ak G, Alatlı F C, Dalay M N. DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES. Sağlık Bilimlerinde İleri Araştırmalar Dergisi. 2022;5(1):20-20. https://doi.org/10.26650/JARHS2021-1142672


    ABNT

    Demokan, S.; Şen, S.; Özemek, B.; Cömert, S.; Eryılmaz, Ö.; Ulusan, M.; Enver, N.; Sezerman, U.; Ak, G.; Alatlı, F.C.; Dalay, M.N. DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES. Sağlık Bilimlerinde İleri Araştırmalar Dergisi, [Publisher Location], v. 5, n. 1, p. 20-20, 2022.


    Chicago: Author-Date Style

    Demokan, Semra, and Sena Şen and Begüm Özemek and Sevde Cömert and Önder Eryılmaz and Murat Ulusan and Necati Enver and Uğur Sezerman and Gülsüm Ak and F. Canan Alatlı and Mehmet Nejat Dalay. 2022. “DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi 5, no. 1: 20-20. https://doi.org/10.26650/JARHS2021-1142672


    Chicago: Humanities Style

    Demokan, Semra, and Sena Şen and Begüm Özemek and Sevde Cömert and Önder Eryılmaz and Murat Ulusan and Necati Enver and Uğur Sezerman and Gülsüm Ak and F. Canan Alatlı and Mehmet Nejat Dalay. DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi 5, no. 1 (Dec. 2022): 20-20. https://doi.org/10.26650/JARHS2021-1142672


    Harvard: Australian Style

    Demokan, S & Şen, S & Özemek, B & Cömert, S & Eryılmaz, Ö & Ulusan, M & Enver, N & Sezerman, U & Ak, G & Alatlı, FC & Dalay, MN 2022, 'DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES', Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 5, no. 1, pp. 20-20, viewed 5 Dec. 2022, https://doi.org/10.26650/JARHS2021-1142672


    Harvard: Author-Date Style

    Demokan, S. and Şen, S. and Özemek, B. and Cömert, S. and Eryılmaz, Ö. and Ulusan, M. and Enver, N. and Sezerman, U. and Ak, G. and Alatlı, F.C. and Dalay, M.N. (2022) ‘DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES’, Sağlık Bilimlerinde İleri Araştırmalar Dergisi, 5(1), pp. 20-20. https://doi.org/10.26650/JARHS2021-1142672 (5 Dec. 2022).


    MLA

    Demokan, Semra, and Sena Şen and Begüm Özemek and Sevde Cömert and Önder Eryılmaz and Murat Ulusan and Necati Enver and Uğur Sezerman and Gülsüm Ak and F. Canan Alatlı and Mehmet Nejat Dalay. DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES.” Sağlık Bilimlerinde İleri Araştırmalar Dergisi, vol. 5, no. 1, 2022, pp. 20-20. [Database Container], https://doi.org/10.26650/JARHS2021-1142672


    Vancouver

    Demokan S, Şen S, Özemek B, Cömert S, Eryılmaz Ö, Ulusan M, Enver N, Sezerman U, Ak G, Alatlı FC, Dalay MN. DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES. Sağlık Bilimlerinde İleri Araştırmalar Dergisi [Internet]. 5 Dec. 2022 [cited 5 Dec. 2022];5(1):20-20. Available from: https://doi.org/10.26650/JARHS2021-1142672 doi: 10.26650/JARHS2021-1142672


    ISNAD

    Demokan, Semra - Şen, Sena - Özemek, Begüm - Cömert, Sevde - Eryılmaz, Önder - Ulusan, Murat - Enver, Necati - Sezerman, Uğur - Ak, Gülsüm - Alatlı, F.Canan - Dalay, MehmetNejat. DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES”. Sağlık Bilimlerinde İleri Araştırmalar Dergisi 5/1 (Dec. 2022): 20-20. https://doi.org/10.26650/JARHS2021-1142672



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