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DOI :10.26650/IUITFD.2020.0093   IUP :10.26650/IUITFD.2020.0093    Tam Metin (PDF)

OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ

Bülent Çakal, Alp Atasoy, Bilger Çavuş, Aslı Çifcibaşı Örmeci, Mehveş Poda, Mesut Bulakçı, Mine Güllüoğlu, Mehmet Güven Günver, Filiz Akyüz

Amaç: Okült Hepatit B Virus (HBV) enfeksiyonu (Occult HBV infection; OBI) mevcut serolojik testler ile HBV yüzey antijeni (Surface antigen; HBsAg) negatif tespit edilen bireylerin karaciğerinde HBV genomunun uzun süreli persistan varlığı ile karakterizedir. Bu çalışmada OBI tanılı hastalardan elde edilen HBV’lerin genotip, subgenotip ve subtip profillerinin karakteristiğinin irdelenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmaya HBsAg negatif farklı klinik endikasyonlar nedeniyle karaciğer parankim biyopsisi gerçekleştirilen hastaların karaciğer biyopsi örneklerinde Nested PCR yöntemiyle HBV DNA varlığı tanımlanan 32 OBI tanılı hasta ile 17 kronik hepatit B hastası dahil edildi. HBV S gen MHR bölgesini hedefleyen DNA fragmanları nested PCR kullanılarak amplifiye edildi. HBV’nin genotip, subgenotip ve subtip/serotiplerinin belirlemek için Sanger sekans metodu kullanıldı. Bulgular: Bu çalışmada OBI ve kronik hepatit B hastalarından izole edilen HBV’lerin tümününün genotipi D, subgenotipler sırasıyla HBV/D1 (%75,5), HBV/D2 (%6,1) ve HBV/D3 (%18,4) serotipler sırasıyla ayw2 (%73,5) ve ayw3 (%26,5) olarak belirlenmiştir. OBI’li hastaların %28,1’nin subgenotipi HBV/D3 olarak belirlenmesine karşın kronik hepatit B hastalarının hiç birinde HBV/D3 subgenotipi saptanmamıştır. Sonuç: Subgenotip HBV/D3’ün viral biyogenezin dinamiği üzerindeki potansiyel etkileri HBsAg negatifliği ve düşük replikasyon yetkinliği ile karakterize OBI ile ilişkili olabilir. 

Anahtar Kelimeler: OBI, Genetik varyasyon, Genotip, Subgenotip, HBsAg Subtipleri, Hepatit B virüsü
DOI :10.26650/IUITFD.2020.0093   IUP :10.26650/IUITFD.2020.0093    Tam Metin (PDF)

ANALYSIS OF GENOTYPE, SUBGENOTYPE AND SEROTYPE OF OCCULT HEPATITIS B VIRUS

Bülent Çakal, Alp Atasoy, Bilger Çavuş, Aslı Çifcibaşı Örmeci, Mehveş Poda, Mesut Bulakçı, Mine Güllüoğlu, Mehmet Güven Günver, Filiz Akyüz

Objective: Occult hepatitis B infection (OBI) can be characterized by the long-term persistence of HBV DNA in the liver of individuals who test negative for the hepatitis B surface antigen (HBsAg) using the currently available assays. This study aimed to evaluate genotype, subgenotype and subtype profiles of HBVs obtained from patients with OBI. Material and Method: The study included 32 patients with OBI who were diagnosed with HBV DNA by Nested PCR method in liver biopsy samples of patients who underwent liver parenchymal biopsy due to HBsAg negative different clinic indications and 17 chronic hepatitis B patients. The MHR region of the HBV S gene was amplified using nested PCR. Sanger sequencing method was used to determine the genotype, subgenotypes and subtype/serotype of HBV. Results: In this study it was determined that the genotypes of all HBVs isolated from OBI and chronic hepatitis B patients were HBV/D, subgenotypes were respectively HBV/ D1 (75.5%), HBV/ D2 (6.1%) and HBV/D3 (18.4%), serotypes were respectively ayw2 (73.5%) and ayw3 (26.5%). Although the subgenotype of 28.1% of patients with OBI was determined as HBV/D3, none of the chronic hepatitis B patients had the HBV/D3 subgenotype. Conclusion: The potential effects of subgenotype HBV/D3 on the dynamics of viral biogenesis may be associated with OBI, characterized by low replication competence and HBsAg negativity.

Anahtar Kelimeler: OBI, Genetic diversity, Genotypes, Subgenotypes, HBsAg Subtypes, Hepatitis B virus

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APA

Çakal, B., Atasoy, A., Çavuş, B., Çifcibaşı Örmeci, A., Poda, M., Bulakçı, M., Güllüoğlu, M., Günver, M.G., & Akyüz, F. (2021). OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ. İstanbul Tıp Fakültesi Dergisi, 84(3), 402-410. https://doi.org/10.26650/IUITFD.2020.0093


AMA

Çakal B, Atasoy A, Çavuş B, Çifcibaşı Örmeci A, Poda M, Bulakçı M, Güllüoğlu M, Günver M G, Akyüz F. OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ. İstanbul Tıp Fakültesi Dergisi. 2021;84(3):402-410. https://doi.org/10.26650/IUITFD.2020.0093


ABNT

Çakal, B.; Atasoy, A.; Çavuş, B.; Çifcibaşı Örmeci, A.; Poda, M.; Bulakçı, M.; Güllüoğlu, M.; Günver, M.G.; Akyüz, F. OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ. İstanbul Tıp Fakültesi Dergisi, [Publisher Location], v. 84, n. 3, p. 402-410, 2021.


Chicago: Author-Date Style

Çakal, Bülent, and Alp Atasoy and Bilger Çavuş and Aslı Çifcibaşı Örmeci and Mehveş Poda and Mesut Bulakçı and Mine Güllüoğlu and Mehmet Güven Günver and Filiz Akyüz. 2021. “OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ.” İstanbul Tıp Fakültesi Dergisi 84, no. 3: 402-410. https://doi.org/10.26650/IUITFD.2020.0093


Chicago: Humanities Style

Çakal, Bülent, and Alp Atasoy and Bilger Çavuş and Aslı Çifcibaşı Örmeci and Mehveş Poda and Mesut Bulakçı and Mine Güllüoğlu and Mehmet Güven Günver and Filiz Akyüz. “OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ.” İstanbul Tıp Fakültesi Dergisi 84, no. 3 (Dec. 2024): 402-410. https://doi.org/10.26650/IUITFD.2020.0093


Harvard: Australian Style

Çakal, B & Atasoy, A & Çavuş, B & Çifcibaşı Örmeci, A & Poda, M & Bulakçı, M & Güllüoğlu, M & Günver, MG & Akyüz, F 2021, 'OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ', İstanbul Tıp Fakültesi Dergisi, vol. 84, no. 3, pp. 402-410, viewed 23 Dec. 2024, https://doi.org/10.26650/IUITFD.2020.0093


Harvard: Author-Date Style

Çakal, B. and Atasoy, A. and Çavuş, B. and Çifcibaşı Örmeci, A. and Poda, M. and Bulakçı, M. and Güllüoğlu, M. and Günver, M.G. and Akyüz, F. (2021) ‘OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ’, İstanbul Tıp Fakültesi Dergisi, 84(3), pp. 402-410. https://doi.org/10.26650/IUITFD.2020.0093 (23 Dec. 2024).


MLA

Çakal, Bülent, and Alp Atasoy and Bilger Çavuş and Aslı Çifcibaşı Örmeci and Mehveş Poda and Mesut Bulakçı and Mine Güllüoğlu and Mehmet Güven Günver and Filiz Akyüz. “OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ.” İstanbul Tıp Fakültesi Dergisi, vol. 84, no. 3, 2021, pp. 402-410. [Database Container], https://doi.org/10.26650/IUITFD.2020.0093


Vancouver

Çakal B, Atasoy A, Çavuş B, Çifcibaşı Örmeci A, Poda M, Bulakçı M, Güllüoğlu M, Günver MG, Akyüz F. OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ. İstanbul Tıp Fakültesi Dergisi [Internet]. 23 Dec. 2024 [cited 23 Dec. 2024];84(3):402-410. Available from: https://doi.org/10.26650/IUITFD.2020.0093 doi: 10.26650/IUITFD.2020.0093


ISNAD

Çakal, Bülent - Atasoy, Alp - Çavuş, Bilger - Çifcibaşı Örmeci, Aslı - Poda, Mehveş - Bulakçı, Mesut - Güllüoğlu, Mine - Günver, MehmetGüven - Akyüz, Filiz. “OKÜLT HEPATİT B VİRÜSÜNÜN GENOTİP, SUBGENOTİP VE SUBTİP ANALİZİ”. İstanbul Tıp Fakültesi Dergisi 84/3 (Dec. 2024): 402-410. https://doi.org/10.26650/IUITFD.2020.0093



ZAMAN ÇİZELGESİ


Gönderim04.08.2020
Kabul04.11.2020
Çevrimiçi Yayınlanma12.07.2021

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