Research Article


DOI :10.26650/ASE2020785091   IUP :10.26650/ASE2020785091    Full Text (PDF)

Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus

Neslihan ŞenerZeliha DemirelEsra İmamoğluMeltem Conk Dalay

Commercial carotenoids of green microalgae have become significant especially for their applications in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of shaking rate, nitrogen concentration and light intensity affect biomass production and the synthesis of carotenoids in the green microalgae were investigated using the statistical design by Box-Behnken (BBD) employing Response Surface Methodology (RSM). Furthermore, the optimized cultivation conditions using BBD for Chlorella vulgaris, Ankistrodesmus convolutus, Dunaliella salina, Tetraselmis striata were determined using the spectrophotometric method to enhance carotenoid concentration. A. convolutus within the green algae was detected with the highest carotenoid concentration. The optimum conditions results indicated that the growth of A. convolutus (0.55 mg/L) and production of total carotenoids (25.1138 mg/g biomass) were found at the stirrer rate of 100 rpm under the light intensity of 100 μE/m2 s, and in the nutrient component of 8.82 mM NaNO3 . These conditions were validated experimentally for total carotenoid yield (24.13 mg/g biomass). After that the production was performed in a flat-plate photobioreactor with a volume of 6L based on the optimized conditions and the carotenoid profile was defined by HPLC-DAD using standards such as violaxanthin, astaxanthin and β-carotene. This study proposes that the RSM approach can be used to define optimal conditions for large-scale production of carotenoids by A. convolutus. 


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APA

Şener, N., Demirel, Z., İmamoğlu, E., & Conk Dalay, M. (2022). Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aquatic Sciences and Engineering, 37(1), 29-37. https://doi.org/10.26650/ASE2020785091


AMA

Şener N, Demirel Z, İmamoğlu E, Conk Dalay M. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aquatic Sciences and Engineering. 2022;37(1):29-37. https://doi.org/10.26650/ASE2020785091


ABNT

Şener, N.; Demirel, Z.; İmamoğlu, E.; Conk Dalay, M. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aquatic Sciences and Engineering, [Publisher Location], v. 37, n. 1, p. 29-37, 2022.


Chicago: Author-Date Style

Şener, Neslihan, and Zeliha Demirel and Esra İmamoğlu and Meltem Conk Dalay. 2022. “Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus.” Aquatic Sciences and Engineering 37, no. 1: 29-37. https://doi.org/10.26650/ASE2020785091


Chicago: Humanities Style

Şener, Neslihan, and Zeliha Demirel and Esra İmamoğlu and Meltem Conk Dalay. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus.” Aquatic Sciences and Engineering 37, no. 1 (Sep. 2022): 29-37. https://doi.org/10.26650/ASE2020785091


Harvard: Australian Style

Şener, N & Demirel, Z & İmamoğlu, E & Conk Dalay, M 2022, 'Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus', Aquatic Sciences and Engineering, vol. 37, no. 1, pp. 29-37, viewed 28 Sep. 2022, https://doi.org/10.26650/ASE2020785091


Harvard: Author-Date Style

Şener, N. and Demirel, Z. and İmamoğlu, E. and Conk Dalay, M. (2022) ‘Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus’, Aquatic Sciences and Engineering, 37(1), pp. 29-37. https://doi.org/10.26650/ASE2020785091 (28 Sep. 2022).


MLA

Şener, Neslihan, and Zeliha Demirel and Esra İmamoğlu and Meltem Conk Dalay. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus.” Aquatic Sciences and Engineering, vol. 37, no. 1, 2022, pp. 29-37. [Database Container], https://doi.org/10.26650/ASE2020785091


Vancouver

Şener N, Demirel Z, İmamoğlu E, Conk Dalay M. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus. Aquatic Sciences and Engineering [Internet]. 28 Sep. 2022 [cited 28 Sep. 2022];37(1):29-37. Available from: https://doi.org/10.26650/ASE2020785091 doi: 10.26650/ASE2020785091


ISNAD

Şener, Neslihan - Demirel, Zeliha - İmamoğlu, Esra - Conk Dalay, Meltem. Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus”. Aquatic Sciences and Engineering 37/1 (Sep. 2022): 29-37. https://doi.org/10.26650/ASE2020785091



TIMELINE


Submitted25.08.2020
Accepted13.05.2021
Published Online29.12.2021

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