Research Article


DOI :10.26650/ASE2020793132   IUP :10.26650/ASE2020793132    Full Text (PDF)

The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations

Mehmet NazGürkan DikenMetin YazıcıYavuz MazlumSelin SayınOktay Söyler

The biochemical compositions and the changes observed in alkaline, neutral and acid protease activities of Artemia enriched with commercial emulsion and different additive combinations were determined. Artemia nauplii (ArtN), GroBiotic-A (GA), Red Algamac (RA), Red Algamac:GroBiotic-A (50:50) (RA:GA (50:50)), Spirulina (SP), Spirulina:Red Algamac (50:50) (SP:RA (50:50)), Spirulina:GroBiotic-A (50:50) (SP:GA (50:50)) and Spirulina:Red Algamac:GroBiotic-A (33:33:33) (SP:RA:GA (33:33:33)) were tested in the study. The lowest and highest protein contents after the enrichment of Artemia were 40.74±1.02% (RA) and 55.03±1.26% (SP:RA:GA (33:33:33)), respectively. The lowest lipid contents of tested groups were found in 5.63±0.47% (GA) and 5.63±0.84% (RA:GA (50:50)). The highest lipid value after the enrichment were observed in 16.98±1.15 % (RA). The lowest and highest ash values observed after the enrichment were 4.51±0.27% (SP:GA (50:50)) and 6.07±0.35% (RA) (p<0.05). The lowest and highest protease activities of the pH=3, pH=4, pH=5, pH=6, pH=7 and pH=8.5 values were 18.18±0.37 U/mg protein (GA), 31.04±0.38 U/mg protein (RA), 9.1±0.32 U/ mg protein (SP), 9.66±0.19 U/mg protein (SP), 16.94±0.61 U/mg protein (SP), 63.09±0.75 U/mg protein (SP) and 33.77±0.59 U/mg protein (RA:GA (50:50)), 57,54±0.34 U/mg protein (RA:GA (50:50)), 23.75±0.28 U/mg protein (GA), 40.82±0.49 U/mg protein (GA), 69.94±0.65 U/mg protein (GA), and 286.14±8.2 U/mg protein (GA) (p<0.05). In conclusion, GA and SP:RA:GA (33:33:33) enrichment combinations are recommended as an alternative to enrichment products. On the other hand, SP and RA should not be used alone due to the disadvantages such as the biochemical composition and proteolytic enzyme activities of Artemia observed in the present study. 


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APA

Naz, M., Diken, G., Yazıcı, M., Mazlum, Y., Sayın, S., & Söyler, O. (2019). The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations. Aquatic Sciences and Engineering, 0(0), 152-158. https://doi.org/10.26650/ASE2020793132


AMA

Naz M, Diken G, Yazıcı M, Mazlum Y, Sayın S, Söyler O. The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations. Aquatic Sciences and Engineering. 2019;0(0):152-158. https://doi.org/10.26650/ASE2020793132


ABNT

Naz, M.; Diken, G.; Yazıcı, M.; Mazlum, Y.; Sayın, S.; Söyler, O. The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations. Aquatic Sciences and Engineering, [Publisher Location], v. 0, n. 0, p. 152-158, 2019.


Chicago: Author-Date Style

Naz, Mehmet, and Gürkan Diken and Metin Yazıcı and Yavuz Mazlum and Selin Sayın and Oktay Söyler. 2019. “The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations.” Aquatic Sciences and Engineering 0, no. 0: 152-158. https://doi.org/10.26650/ASE2020793132


Chicago: Humanities Style

Naz, Mehmet, and Gürkan Diken and Metin Yazıcı and Yavuz Mazlum and Selin Sayın and Oktay Söyler. The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations.” Aquatic Sciences and Engineering 0, no. 0 (May. 2021): 152-158. https://doi.org/10.26650/ASE2020793132


Harvard: Australian Style

Naz, M & Diken, G & Yazıcı, M & Mazlum, Y & Sayın, S & Söyler, O 2019, 'The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations', Aquatic Sciences and Engineering, vol. 0, no. 0, pp. 152-158, viewed 15 May. 2021, https://doi.org/10.26650/ASE2020793132


Harvard: Author-Date Style

Naz, M. and Diken, G. and Yazıcı, M. and Mazlum, Y. and Sayın, S. and Söyler, O. (2019) ‘The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations’, Aquatic Sciences and Engineering, 0(0), pp. 152-158. https://doi.org/10.26650/ASE2020793132 (15 May. 2021).


MLA

Naz, Mehmet, and Gürkan Diken and Metin Yazıcı and Yavuz Mazlum and Selin Sayın and Oktay Söyler. The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations.” Aquatic Sciences and Engineering, vol. 0, no. 0, 2019, pp. 152-158. [Database Container], https://doi.org/10.26650/ASE2020793132


Vancouver

Naz M, Diken G, Yazıcı M, Mazlum Y, Sayın S, Söyler O. The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations. Aquatic Sciences and Engineering [Internet]. 15 May. 2021 [cited 15 May. 2021];0(0):152-158. Available from: https://doi.org/10.26650/ASE2020793132 doi: 10.26650/ASE2020793132


ISNAD

Naz, Mehmet - Diken, Gürkan - Yazıcı, Metin - Mazlum, Yavuz - Sayın, Selin - Söyler, Oktay. The changes in Alkaline, Neutral and Acid Protease Activities of Artemia Enriched with Commercial Emulsion and Different Additive Combinations”. Aquatic Sciences and Engineering 0/0 (May. 2021): 152-158. https://doi.org/10.26650/ASE2020793132



TIMELINE


Submitted10.09.2020
Accepted15.12.2020
Published Online20.04.2021

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