Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential
Truong Quoc Tat, Nguyen Duy KhanhObjective: This study aims to demonstrate the potential reuse of Cau banana peel (CBP), a fruit by-product in the Mekong Delta, Vietnam, based on its antioxidant and antibacterial activity.
Materials and Methods: The determination of optimal extraction conditions for phenolics using response surface methodology from CBP powder. Then, the CBP extract was evaluated for total phenolic content (TPC), flavonoid content (TFC), phytochemical constituents, and antioxidant activity by the DPPH free radical scavenging assay, whereas antibacterial effects against the pathogens Staphylococcus aureus and Escherichia coli were determined by the agar diffusion and broth dilution methods.
Results: The highest TPC (38.42 mgGAE/g) and TFC (9.75 mgQE/g) were achieved with 1 g of the CBP powder, 58 mL of 60% ethanol solution, and 78 min of processing time at 56°C. The CBP extract contained tannins, flavonoids, alkaloids, saponins, and glycosides, with TPC and TFC of 543.48 mgGAE/g and 158.96 mgQE/g, respectively. The IC50 was 105.85 μg/mL, and the MBC/MIC ratios were ≤2.0 mg/mL. Therefore, it could be a strong antioxidant and bactericidal agent.
Conclusion: These results show that the peel waste of CBP could be helpful in the medical or health food industry for various applications instead of dumping them in landfills.
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References
- 1. Gonzalez-Montelongo R, Lobo MG, Gonzalez M. Antioxidant activity in banana peel extracts: Testing extraction conditions and related bioactive compounds. Food Chem. 2010;119(3):1030-1039. google scholar
- 2. Isibika A, Vinneras B, Kibazohi O, Zurbrügg C, Lalander C. Co-composting of banana peel and orange peel waste with fish waste to improve conversion by black soldier fly (Hermetia illucens (L.), Diptera: Stratiomyidae) larvae. J Clean Prod. 2021;318:128570. doi:10.1016/j.jclepro.2021.128570 google scholar
- 3. Budhalakoti N. Extraction of protein from banana by-product and its characterization. J Food Meas Charact. 2021;15(3):2202-2210. google scholar
- 4. Palacios AS, Llyina A, Ramos-Gonzalez R, et al. Ethanol pro-duction from banana peels at high pretreated substrate loading: comparison of two operational strategies. Biomass Conv Bioref. 2021;11:1587-1596. google scholar
- 5. Unakal C, Kallur RI, Kaliwal BB. Production of a-amylase using banana waste by Bacillus subtilis under solid state fermentation. Eur J Exp Biol. 2012;2:1044-1052. google scholar
- 6. Eshak NS. Sensory evaluation and nutritional value of balady flat bread supplemented with banana peels as a natural source of dietary fiber. Ann Agric Sci. 2016;61(2):229-235. google scholar
- 7. Kumar KS, Bhowmik D, Duraivel S, Umadevi M. Traditional and medicinal uses of banana. J Pharmacogn Phytochem. 2012;1(3):51-63. google scholar
- 8. Hikal WM, Ahl SA, Hussein AH et al. Banana peels: A waste treasure for human being. Evid Based Complement Alternat Med. 2022;7616452. doi:10.1155/2022/7616452 google scholar
- 9. Vu HT, Scarlett CJ, Vuong QV. Phenolic compounds within banana peel and their potential uses: A review. J Funct Foods. 2018;40:238-248. google scholar
- 10. Zaini HM, Roslan J, Saallah S, et al. Banana peels as a bioactive ingredient and its potential application in the food industry. J Funct Foods. 2022;92:105054. doi:10.1016/j.jff.2022.105054. google scholar
- 11. Sundaram S, Anjum S, Dwivedi P, Rai GK. Antioxidant activity and protective effect of banana peel against oxidative hemolysis of human erythrocyte at different stages of ripening. Biotechnol Appl Biochem. 2011;64:1192-1206. google scholar
- 12. Yan L, Fernando WM, Brennan M, et al. Effect of extraction method and ripening stage on banana peel pigments. Int J Food Sci Technol. 2016;51(6):1449-1456. google scholar
- 13. Hikal WM, Said-Al, Ahl HA. Banana peels as possible an-tioxidant and antimicrobial agents. Asian J Res Rev Agrc. 2021;3(3):35-45. google scholar
- 14. Alam MJ, Akter S, Afroze S, Islam MT, Sayeem EH. Devel-opment of fiber and mineral enriched cookies by utilization of banana and banana peel flour. J Microbiol Biotechnol Food Sci. 2020;10(3):329-334. google scholar
- 15. Singh B, Singh JP, Kaur A, Singh N. Bioactive compounds in banana and their associated health benefits-A review. Food Chem. 2016;206:1-11. google scholar
- 16. Crozie A, Jaganath IB, Clifford MN. Phenols, polyphenols and tannins: an overview. Plant secondary metabolites: Occurrence, structure and role in the human diet 2006;1:1-25. google scholar
- 17. Pereira GA, Molina G, Arruda HS, Pastore GM. Optimiz-ing the homogenizer-assisted extraction (HAE) of total phe-nolic compounds from banana peel. J Food Process Eng. 2017;40(3):e12438. doi:10.1111/jfpe.12438 google scholar
- 18. Anhwange BA, Ugye TJ, Nyiaatagher TD. Chemical composi-tion of Musa sapientum (banana) peels. Electron J Environ Agr Food Chem. 2009;8(6):437-442. google scholar
- 19. Chinemerem ND, Ugwu MC, Oliseloke AC, et al. Antibiotic resistance: The challenges and some emerging strategies for tackling a global menace. J Clin Lab Anal. 2022;36(9):e24655. doi:10.1002/jcla.24655 google scholar
- 20. Atef NM, Shanab SM, Negm SI, Abbas YA. Evaluation of an-timicrobial activity of some plant extracts against antibiotic sus-ceptible and resistant bacterial strains causing wound infection. Bull Natl Res Cent. 2019;43(1):1-11. google scholar
- 21. Mpharm SR. Anti-inflammatory and antioxidant activities of extracts from Musa sapientum peel. J Med Assoc Thai. 2012;95(1):142-146. google scholar
- 22. Kumari P, Pillai VV, Benedetto A. Mechanisms of action of ionic liquids on living cells: The state of the art. Biophys Rev. 2020;12(5):1187-1215. google scholar
- 23. Kapadia SP, Pudakalkatti S, Shivanaikar S. Detection of an-timicrobial activity of banana peel (Musa paradisiaca L.) on Porphyromonas gingivalis and Aggregatibacter actinomycetem-comitans: An in vitro study. Contemp Clin Dent. 2015;6(4):496-499. google scholar
- 24. Tai NV, Minh VQ, Thuy NM. Food processing waste in Vietnam: Utilization and prospects in food industry for sustainability de-velopment. J Microbiol Biotechnol Food Sci. 2023;13(1):e9926. doi:10.55251/jmbfs.9926 google scholar
- 25. Tai NV, Linh MN, Thuy NM. Optimization of extraction condi-tions of phytochemical compounds in “Xiem” banana peel pow-der using response surface methodology. J App Biol Biotech. 2021;9(6):56-62. google scholar
- 26. Sofowora A. Screening plants for bioactive agents. Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Spectrum Books Ltd, Sunshine House, Ibadan, Nigeria, 1993:134-156. google scholar
- 27. Tiwari P, Kumar B, Kaur M, Kaur GH. Phytochemical screening and extraction: A review. Int Pharm Sci. 2011;1(1): 98-106. google scholar
- 28. Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol. 2011;3(12):10-14. google scholar
- 29. Mandal B, Madan S. Preliminary phytochemical screen-ing and evaluation of free radical scavenging activity of Stevia rebaudiana Bertoni from different geographical sources. J Pharmacogn Phytochem. 2013;2(1):14-19. google scholar
- 30. Navghare VV, Dhawale SC. In vitro antioxidant, hypoglycemic and oral glucose tolerance test of banana peels. Alex J Med. 2017;53(3):237-243. google scholar
- 31. Natta L, Orapin K, Krittika N, Pantip B. Essential oil from five Zingiberaceae for anti food-borne bacteria. Int Food Res J. 2008;15(3):337-46. google scholar
- 32. Setyowati WAE, Susanti VHE. Antibacterial activity of natural paper from banana peel (Musa paradisiaca Linn.) with additive essential oils. In IOP Conference Series: Materials Science and Engineering. 2019;578(1):012048. IOP Publishing. google scholar
- 33. Indriani V, Chiuman L, Wijaya LL, et al. Antibacterial ef-fect of Curcuma zedoaria extract on Bacillus cereus and Staphylococcus epidermidis. Althea Med J. 2020;7(1):6-10. google scholar
- 34. Cheesbrough M. District laboratory practice in tropical coun-tries, Part 2. Cambridge university Press, United Kingdom 2005. google scholar
- 35. Singhal M, Ratra P. Antioxidant activity, total flavonoid and total phenolic content of Musa acuminate peel extracts. Global J Pharmacol. 2013;7(2):118-122. google scholar
- 36. Ishak NA, Raza NAA, Dek SP, Baharuddin AS. Production of high tannin content and antioxidant activity extract from an unripe peel of Musa acuminata (Cavendish) using ultrasound-assisted extraction (UAE). BioRes. 2020;15(1):1877-1893. google scholar
- 37. Said FM, Gan JY, Sulaiman J. Correlation between response sur-face methodology and artificial neural network in the prediction of bioactive compounds of unripe Musa acuminata peel. Eng Sci Technol Int. 2020;3(4):781-787. google scholar
- 38. Anal AK, Jaisanti S, Noomhorm A. Enhanced yield of phenolic extracts from banana peels (Musa acuminata Colla AAA) and cinnamon barks (Cinnamomum varum) and their antioxidative potentials in fish oil. J Food Sci Technol. 2014;51:2632-2639. google scholar
- 39. Lavelli V, Caronni P. Polyphenol oxidase activity and implica-tions on the quality of intermediate moisture and dried apples. Eur Food Res Technol. 2010;231:93-100. google scholar
- 40. Sulastri E, Zubair MS, Anas NI, et al. Total phenolic, total flavonoid, quercetin content and antioxidant activity of standard-ized extract of Moringa oleifera leaf from regions with different elevation. Pharmacogn J. 2018;10(6):104-108. google scholar
- 41. Fidrianny I, Suhendy H, Insanu M. Correlation of phytochem-ical content with antioxidant potential of various sweet potato (Ipomoea batatas) in West Java, Indonesia. Asian Pac J Trop Biomed. 2018;8(1):25-30. google scholar
- 42. Salim E. Dried samples characterization and determination of total phenol and flavonoid of ethanol extract from Musa paradisiaca L. fruit peel. Na J Adv Res Rev. 2022;16(3):941-946. google scholar
- 43. Kabir MR, Hasan MM, Islam MR, et al. Formulation of yo-gurt with banana peel extracts to enhance storability and bioac-tive properties. J Food Process Preserv. 2021;45(3):e15191. doi:10.1111/jfpp.15191 google scholar
- 44. Sharma K, Kumar V, Kaur J, et al. Health effects, sources, utilization and safety of tannins: A critical review. Toxin Rev. 2021;40(4):432-444. google scholar
- 45. Salvamani S, Gunasekaran B, Shaharuddin NA, et al. An-tiartherosclerotic effects of plant flavonoids. Biomed Res Int. 2014;480258. doi:10.1155/2014/480258 google scholar
- 46. Bribi N. Pharmacological activity of alkaloids: A review. Asian J Bot. 2018;1(1):1-6. google scholar
- 47. Zarin MA, Wan HY, Isha A, Armania, N. Antioxidant, antimicrobial and cytotoxic potential of condensed tannins from Leucaena leucocephala hybrid-Rendang. Food Sci Hum Wellness. 2016;5(2):65-75. google scholar
- 48. Sharmin F, Koyama T, Koyama H, Ishizaki S. Cholesterol-binding ability of saponin from Japanese starfish. J Food Sci Technol. 2021;58:3056-3064. google scholar
- 49. Marrelli M, Conforti F, Araniti F, Statti GA. Effects of saponins on lipid metabolism: A review of potential health bene-fits in the treatment of obesity. Molecules. 2016;21(10):1404. doi:10.3390/molecules21101404 google scholar
- 50. Riaz T, Akram M, Laila U, et al. Therapeutic applications of glycosides obtained from medicinal plants. Int Arch Integr Med. 2023;10(8):30-38. google scholar
- 51. Siddique S, Nawaz S, Muhammad F, et al. Phytochemical screen-ing and in-vitro evaluation of pharmacological activities of peels of Musa sapientum and Carica papaya fruit. Nat Prod Res. 2018;32(11):1333-1336. google scholar
- 52. Molyneux P. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin. J Sci Technol. 2004;26(2):211-219. google scholar
- 53. Ariani N, Nurani LH. The antioxidant activity analysis of the ethanolic extract of banana peel (Musa paradisiaca forma typica) with DPPH method. In Proceedings of the 1st Muhammadiyah International Conference on Health and Pharmaceutical Development. 2018;2018, 44-47. google scholar
- 54. Rit WS, Swantara IMD, Asih IARA, Puspawati NM. Antibac-terial activity and antioxidant capacity of selected local banana peel (Musa sp.) methanol extracts cultivated in Bali. Int J Agri Env Biotech. 2020;5(03):242-251. google scholar
- 55. Behiry SI, Okla MK, Alamri SA, et al. Antifungal and antibacte-rial activities of Musa paradisiaca L. peel Extract: HPLC anal-ysis of phenolic and flavonoid contents. Processes. 2019;7:215. doi:10.3390/pr7040215 google scholar
- 56. Abdel Aziz SM, Ahmed OM, Abd EL-Twab SM, et al. Antihyperglycemic effects and mode of actions of Musa paradisiaca leaf and fruit peel hydroethanolic ex-tracts in nicotinamide/streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med. 2020;2020:9276343. doi:10.1155/2020/9276343 google scholar
- 57. Kumari PS, Ranjitha R, Vidhya N. Revitalizing property of banana peel extracts by antioxidant activity and antibacterial activity against acne causing Staphylococcus epidermidis. Ann Phytomed. 2020;9(2):215-222. google scholar
- 58. Hashim M, Hamid Z, Gul Z, Akbar A. Functional, nutri-tional and medicinal potential of banana peel. Pure Appl Bio. 2023;12(1):470-490. google scholar
- 59. Ruangtong J, Jiraroj T, T-Thienpraser NP. Green synthe-sized ZnO nanosheets from banana peel extract possess anti-bacterial activity and anti-cancer activity. Mater Today Commun. 2020;24:101224. doi:10.1016/j.mtcomm.2020.101224 google scholar
- 60. 60. Azarudeen AM, Nithya R. Pharmaceutical aspects of banana peel: A review. J Pharm Sci Res. 2021;13(2):112-117. google scholar
- 61. Indriani V, Chiuman L, Wijaya LL, et al. Antibacterial ef-fect of Curcuma zedoaria extract on Bacillus cereus and Staphylococcus epidermidis. Althea Med J. 2020;7(1):6-10. google scholar
- 62. Mitscher LA, Leu RP, Bathal MS, et al. Antimicrobial agents from higher plants. I. Introduction, rationale, and methodology. Lloydia. 1972;35(2):157-166. google scholar
- 63. Gas EN, Aho HS, Borquaye LS. Peptide extract from Olivancillaria hiatula exhibits broad-spectrum antibac-terial activity. Biomed Res Int. 2018;2018:6010572. doi:10.1155/2018/6010572 google scholar
- 64. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evalu-ating antimicrobial activity: A review. J Pharm Anal. 2016;6:71-79. google scholar
- 65. Mekinic IG, Skroza D, Ljubenkov I, et al. Antioxidant and an-timicrobial potential of phenolic metabolites from traditionally used mediterranean herbs and spices. Foods. 2019;8:1-16. google scholar
- 66. Fajrih N, Wiryawan KG, Sumiati S, et al. Identification of bioac-tive compounds of banana corm (Musa paradisiaca) using GC-MS and its inhibitory effect against pathogenic bacteria. Biodiv J Biol Div. 2022;23(1):195-204. google scholar
- 67. Vollmer W. "Peptidoglycan." Molecular Medical Microbiology. Academic Press, 2015. 105-124. google scholar
- 68. Yahaya A, Ali M, EL-Hassan FI, Jido BA. Antibacterial activity of guava (Psidium guajava L.) extracts on Staphylococcus aureus isolated from patients with urinary tract infections attending a tertiary-care hospital. Sci World J. 2019;14(1):47-51. google scholar
- 69. Sabir A. Antibacterial activity of flavonoid from propolis trig-ona sp against Streptococcus mutans bacteria (in vitro). Dent J. 2005;38(3):135-141. google scholar
- 70. Cushnie TT, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents. 2011;38:99-107. google scholar
- 71. Thawabteh A, Juma S, Bader M, et al. The biological activ-ity of natural alkaloids against herbivores, cancerous cells and pathogens. Toxins. 2019;11:1-28. google scholar
- 72. Sandhiutami NMD, Fahleni F, Miftahurrohmah N, et al. En-hanced wound healing effect of Areca catechu L. ointment via antibacterial activity and anti-inflammatory process at grade IIA burns in rats. J Herbmed Pharmacol. 2023;12(3):388-398. google scholar
- 73. Arabski M, Wasik S, Dworecki K, Kaca W. Laser inter-ferometric and cultivation methods for measurement of col-istin/ampicilin and saponin interactions with smooth and rough of Proteus mirabilis lipopolysaccharides and cells. J Microbiol Methods. 2009;77:178-83. google scholar
- 74. Meliawaty F, Fadilah RPN, Mentari P. Ambon banana peel ex-tract gel (Musa paradisiaca var. Sapientum (L.) Kunt) acce-larates of wistar rats gingiva wound healing. J Health Dent Sci. 2021;1(1):90-100. google scholar
- 75. Savitri D, Djawad K, Hatta M, et al. Active compounds in kepok banana peel as anti-inflammatory in acne vul-garis: Review article. Ann Med Surg (Lond). 2022;84:104868. doi:10.1016/j.amsu.2022.104868 google scholar
- 76. Okorondu SI, Mepba HD, Okorondu MMO, Aririat LE. Antibacterial properties of Musa paradisiaca peel extract. Curr Trends Microbiol. 2010;6:21-26. google scholar
- 77. Asoso O, Akharaiyi FC, Animba L. Antibacterial activities of plantain (Musa paradisiaca) peel and fruit. Der Pharm Lett. 2016;8(5):5-11. google scholar
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APA
Tat, T.Q., & Khanh, N.D. (2024). Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential. European Journal of Biology, 0(0), -. https://doi.org/10.26650/EurJBiol.2024.1459106
AMA
Tat T Q, Khanh N D. Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential. European Journal of Biology. 2024;0(0):-. https://doi.org/10.26650/EurJBiol.2024.1459106
ABNT
Tat, T.Q.; Khanh, N.D. Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential. European Journal of Biology, [Publisher Location], v. 0, n. 0, p. -, 2024.
Chicago: Author-Date Style
Tat, Truong Quoc, and Nguyen Duy Khanh. 2024. “Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential.” European Journal of Biology 0, no. 0: -. https://doi.org/10.26650/EurJBiol.2024.1459106
Chicago: Humanities Style
Tat, Truong Quoc, and Nguyen Duy Khanh. “Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential.” European Journal of Biology 0, no. 0 (Oct. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1459106
Harvard: Australian Style
Tat, TQ & Khanh, ND 2024, 'Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential', European Journal of Biology, vol. 0, no. 0, pp. -, viewed 11 Oct. 2024, https://doi.org/10.26650/EurJBiol.2024.1459106
Harvard: Author-Date Style
Tat, T.Q. and Khanh, N.D. (2024) ‘Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential’, European Journal of Biology, 0(0), pp. -. https://doi.org/10.26650/EurJBiol.2024.1459106 (11 Oct. 2024).
MLA
Tat, Truong Quoc, and Nguyen Duy Khanh. “Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential.” European Journal of Biology, vol. 0, no. 0, 2024, pp. -. [Database Container], https://doi.org/10.26650/EurJBiol.2024.1459106
Vancouver
Tat TQ, Khanh ND. Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential. European Journal of Biology [Internet]. 11 Oct. 2024 [cited 11 Oct. 2024];0(0):-. Available from: https://doi.org/10.26650/EurJBiol.2024.1459106 doi: 10.26650/EurJBiol.2024.1459106
ISNAD
Tat, TruongQuoc - Khanh, NguyenDuy. “Optimization of Extraction Parameters of Phenolic Compounds from Cau Banana (Musa paradisiaca) Peel and Evaluation for Antioxidant and Antibacterial Potential”. European Journal of Biology 0/0 (Oct. 2024): -. https://doi.org/10.26650/EurJBiol.2024.1459106