Assessing E-Cadherin and Connexin 43 Gene  Expressions in Colorectal Cancer

Sürmen Saime; Arıkan Soykan; Timirci Kahraman Özlem; Sürmen Mustafa Gani; Cacina Canan; Yaylım İlhan

doi:https://dx.doi.org/10.26650/experimed.1189327

Research Article

DOI :10.26650/experimed.1189327 IUP :10.26650/experimed.1189327 Full Text (PDF)

Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer

Saime Sürmen, Soykan Arıkan, Özlem Timirci Kahraman, Mustafa Gani Sürmen, Canan Cacina, İlhan Yaylım

Objective: Dysregulation of cellular adhesion is one of the main mechanisms responsible for tumor initiation, proliferation, and survival. E-cadherin is a cell adhesion molecule associated with tissue invasion and metastasis in most epithelial cancers. Gap junctions are known as small molecular channels that allow communication between neighboring cells and consist of connexin molecules. Connexin 43 (Cx43) is a gap junction protein that plays a central role in cell-cycle regulation and has an important function in carcinogenesis. The present study aimed to evaluate the expression levels of E-cadherin and Cx43 in colorectal cancer patients using clinical and prognostic parameters. Materials and Methods: The quantitative real-time polymerase chain reaction (qRT-PCR) method was utilized to characterize the expression patterns of the E-cadherin and Cx43 genes in tumor and adjacent non-tumoral colon tissues from 32 colorectal cancer patients. Analysis of gene expression data was carried out using the delta-CT method. Results: The results show the expression level of Cx43 to decrease 14-fold in tumor tissue compared to normal tissue (p<0.05). However, the study could find no significant difference with regard to E-cadherin expression. Conclusion: The research provides valuable clues to the elucidation of tumor development and metastatic processes for further studies.

Keywords: Colorectal cancer, cell adhesion, connexin 43, E-cadherin, qRT-PCR

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APA

Sürmen, S., Arıkan, S., Timirci Kahraman, Ö., Sürmen, M., Cacina, C., & Yaylım, İ. (2022). Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer. Experimed, 12(3), 119-124. https://doi.org/10.26650/experimed.1189327

AMA

Sürmen S, Arıkan S, Timirci Kahraman Ö, Sürmen M, Cacina C, Yaylım İ. Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer. Experimed. 2022;12(3):119-124. https://doi.org/10.26650/experimed.1189327

ABNT

Sürmen, S.; Arıkan, S.; Timirci Kahraman, Ö.; Sürmen, M.; Cacina, C.; Yaylım, İ. Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer. Experimed, [Publisher Location], v. 12, n. 3, p. 119-124, 2022.

Chicago: Author-Date Style

Sürmen, Saime, and Soykan Arıkan and Özlem Timirci Kahraman and Mustafa Gani Sürmen and Canan Cacina and İlhan Yaylım. 2022. “Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer.” Experimed 12, no. 3: 119-124. https://doi.org/10.26650/experimed.1189327

Chicago: Humanities Style

Sürmen, Saime, and Soykan Arıkan and Özlem Timirci Kahraman and Mustafa Gani Sürmen and Canan Cacina and İlhan Yaylım. “Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer.” Experimed 12, no. 3 (Sep. 2024): 119-124. https://doi.org/10.26650/experimed.1189327

Harvard: Australian Style

Sürmen, S & Arıkan, S & Timirci Kahraman, Ö & Sürmen, M & Cacina, C & Yaylım, İ 2022, 'Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer', Experimed, vol. 12, no. 3, pp. 119-124, viewed 9 Sep. 2024, https://doi.org/10.26650/experimed.1189327

Harvard: Author-Date Style

Sürmen, S. and Arıkan, S. and Timirci Kahraman, Ö. and Sürmen, M. and Cacina, C. and Yaylım, İ. (2022) ‘Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer’, Experimed, 12(3), pp. 119-124. https://doi.org/10.26650/experimed.1189327 (9 Sep. 2024).

MLA

Sürmen, Saime, and Soykan Arıkan and Özlem Timirci Kahraman and Mustafa Gani Sürmen and Canan Cacina and İlhan Yaylım. “Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer.” Experimed, vol. 12, no. 3, 2022, pp. 119-124. [Database Container], https://doi.org/10.26650/experimed.1189327

Vancouver

Sürmen S, Arıkan S, Timirci Kahraman Ö, Sürmen M, Cacina C, Yaylım İ. Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer. Experimed [Internet]. 9 Sep. 2024 [cited 9 Sep. 2024];12(3):119-124. Available from: https://doi.org/10.26650/experimed.1189327 doi: 10.26650/experimed.1189327

ISNAD

Sürmen, Saime - Arıkan, Soykan - Timirci Kahraman, Özlem - Sürmen, Mustafa Gani - Cacina, Canan - Yaylım, İlhan. “Assessing E-Cadherin and Connexin 43 Gene Expressions in Colorectal Cancer”. Experimed 12/3 (Sep. 2024): 119-124. https://doi.org/10.26650/experimed.1189327

Volume 12, Issue 32022, P. 119-124

TIMELINE

Submitted	16.10.2022
Accepted	21.11.2022
Published Online	27.12.2022

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