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DOI :10.26650/experimed.1319722   IUP :10.26650/experimed.1319722    Full Text (PDF)

Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells

Rengin Reis, Kübra Kolci

Objective: Thirdhand smoke (THS) is described as the accumulated chemicals left on indoor surfaces after tobacco smoking. Individuals can be exposed to THS by dermal or oral contact with THS-embedded surfaces or by breathing in the off-gasses. In the present study, the cytotoxic, oxidative, and inflammatory responses of THS extracts yielded from conventional (THS-C) and heated-tobacco products (THS-H) were examined in BEAS-2B human bronchial epithelial cell line.

Materials and Methods: The terrycloth samples were exposed to smoke in a closed polystyrene box and extracted in a complete cell culture medium for 24 hours at 37°C. Following this, the cytotoxicity of THS was assessed by MTT assay. Malondialdehyde (MDA) and intracellular glutathione (GSH) levels were determined in BEAS-2B cell lysate to assess oxidative response. The aryl hydrocarbon receptor

(AhR) and interleukine-6 (IL-6) levels were determined via an ELISA kit.

Results: Both types of THS led to dose-dependent cytotoxicity in cells, which was remarkable with THS-C (50%, v/v). Moreover, GSH depletion and MDA increase were remarkable with both THS, particularly with THS-C. AhR activation was also slightly elevated with THS-C, whereas the increase in IL-6 was notable compared to THS-H.

Conclusion: THS exposure might lead to potential health risks particularly for respiratory health and the results support the need for comprehensive regulations and public health initiatives to minimize the harmful effects of THS.

Keywords: Thirdhand smoke, heated-tobacco product, oxidative stress, aryl hydrocarbon receptor, inflammation, interleukine-6.

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APA

Reis, R., & Kolci, K. (2023). Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells. Experimed, 13(2), 127-132. https://doi.org/10.26650/experimed.1319722


AMA

Reis R, Kolci K. Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells. Experimed. 2023;13(2):127-132. https://doi.org/10.26650/experimed.1319722


ABNT

Reis, R.; Kolci, K. Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells. Experimed, [Publisher Location], v. 13, n. 2, p. 127-132, 2023.


Chicago: Author-Date Style

Reis, Rengin, and Kübra Kolci. 2023. “Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells.” Experimed 13, no. 2: 127-132. https://doi.org/10.26650/experimed.1319722


Chicago: Humanities Style

Reis, Rengin, and Kübra Kolci. “Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells.” Experimed 13, no. 2 (Dec. 2023): 127-132. https://doi.org/10.26650/experimed.1319722


Harvard: Australian Style

Reis, R & Kolci, K 2023, 'Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells', Experimed, vol. 13, no. 2, pp. 127-132, viewed 8 Dec. 2023, https://doi.org/10.26650/experimed.1319722


Harvard: Author-Date Style

Reis, R. and Kolci, K. (2023) ‘Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells’, Experimed, 13(2), pp. 127-132. https://doi.org/10.26650/experimed.1319722 (8 Dec. 2023).


MLA

Reis, Rengin, and Kübra Kolci. “Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells.” Experimed, vol. 13, no. 2, 2023, pp. 127-132. [Database Container], https://doi.org/10.26650/experimed.1319722


Vancouver

Reis R, Kolci K. Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells. Experimed [Internet]. 8 Dec. 2023 [cited 8 Dec. 2023];13(2):127-132. Available from: https://doi.org/10.26650/experimed.1319722 doi: 10.26650/experimed.1319722


ISNAD

Reis, Rengin - Kolci, Kübra. “Comparative Toxicity Responses of Thirdhand Smoke Derived from Conventional Cigarette and Heated Tobacco Products in Human Bronchial Epithelial Cells”. Experimed 13/2 (Dec. 2023): 127-132. https://doi.org/10.26650/experimed.1319722



TIMELINE


Submitted25.06.2023
Accepted27.07.2023
Published Online31.07.2023

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