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DOI :10.26650/experimed.1172749   IUP :10.26650/experimed.1172749    Full Text (PDF)

Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?

Sertaç Argun Kıvanç, Berna Akova, Merih Kıvanç

Objective: Our aim was to investigate the formation of Staphylococcus (S.) epidermidis biofilm on hydrophobic acrylic lenses and whether the inhibition of the formed biofilm is possible with probiotic Lactobacillus (L.) rhamnosus 312 and parabiotic prepared from it. Materials and Methods: The probiotic bacteria L. rhamnosus 312 and intercellular adhesion (ICA) gene-positive tested bacteria S. epidermidis KA15.8 were used in the study from stock. To obtain the parabiotic the cultures were developed in De Man Rogosa and Sharpe (MRS) broth for 48 hours and autoclaved at 121ºC for 15 minutes. Biofilms on hydrophobic acrylic intraocular lenses and the antibiofilm effects of parabiotic and probiotic L. rhamnosus were evaluated. Scanning electron microscopy photos of biofilms produced on intraocular lenses (IOLs) were taken. Results: Probiotic L. rhamnosus 312 and the parabiotic test showed antibacterial activity on test bacteria, ICA positive S. epidermidis KA15.8. However, the probiotic L. rhamnosus 312 zone diameter was found to be wider. After the biofilm was formed, the addition of parabiotic inhibited the biofilm formed by S. epidermis KA15.8 by 58.29%. The number of S. epidermidis KA15.8 in the biofilm also decreased. Conclusion: Parabiotic and probiotic L. rhamnosus 312 was found effective for its antibiofilm effect. However, further studies with different concentrations are needed.

Keywords: Probiotic, parabiotic, ophthalmology, cataract, intraocular lens, biofilm

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APA

Kıvanç, S.A., Akova, B., & Kıvanç, M. (2022). Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?. Experimed, 12(3), 103-107. https://doi.org/10.26650/experimed.1172749


AMA

Kıvanç S A, Akova B, Kıvanç M. Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?. Experimed. 2022;12(3):103-107. https://doi.org/10.26650/experimed.1172749


ABNT

Kıvanç, S.A.; Akova, B.; Kıvanç, M. Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?. Experimed, [Publisher Location], v. 12, n. 3, p. 103-107, 2022.


Chicago: Author-Date Style

Kıvanç, Sertaç Argun, and Berna Akova and Merih Kıvanç. 2022. “Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?.” Experimed 12, no. 3: 103-107. https://doi.org/10.26650/experimed.1172749


Chicago: Humanities Style

Kıvanç, Sertaç Argun, and Berna Akova and Merih Kıvanç. “Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?.” Experimed 12, no. 3 (Sep. 2024): 103-107. https://doi.org/10.26650/experimed.1172749


Harvard: Australian Style

Kıvanç, SA & Akova, B & Kıvanç, M 2022, 'Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?', Experimed, vol. 12, no. 3, pp. 103-107, viewed 9 Sep. 2024, https://doi.org/10.26650/experimed.1172749


Harvard: Author-Date Style

Kıvanç, S.A. and Akova, B. and Kıvanç, M. (2022) ‘Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?’, Experimed, 12(3), pp. 103-107. https://doi.org/10.26650/experimed.1172749 (9 Sep. 2024).


MLA

Kıvanç, Sertaç Argun, and Berna Akova and Merih Kıvanç. “Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?.” Experimed, vol. 12, no. 3, 2022, pp. 103-107. [Database Container], https://doi.org/10.26650/experimed.1172749


Vancouver

Kıvanç SA, Akova B, Kıvanç M. Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?. Experimed [Internet]. 9 Sep. 2024 [cited 9 Sep. 2024];12(3):103-107. Available from: https://doi.org/10.26650/experimed.1172749 doi: 10.26650/experimed.1172749


ISNAD

Kıvanç, SertaçArgun - Akova, Berna - Kıvanç, Merih. “Do Lactobacillus rhamnosus-Originated Probiotic and Parabiotic Have Inhibitory Effects on Intraocular Lens Biofilm?”. Experimed 12/3 (Sep. 2024): 103-107. https://doi.org/10.26650/experimed.1172749



TIMELINE


Submitted08.09.2022
Accepted12.10.2022
Published Online29.12.2022

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