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DOI :10.26650/experimed.1268503   IUP :10.26650/experimed.1268503    Full Text (PDF)

Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish

Gizem Gündüz, Merih Beler, Ismail Unal, Derya Cansız, Ebru Emekli Alturfan, Kemal Naci Köse

Objective: Porphyromonas gingivalis (P. gingivalis), a major periodontopathogen, is associated with overfeeding disorders, including metabolic syndrome. Gingipains are one of the most powerful endotoxins of P. gingivalis. Our aim was to reveal the effects of gingipain injections on the intestinal oxidant-antioxidant status and alkaline phosphatase (ALP) activity in overfed zebrafish.

Materials and Methods: Four groups of healthy adult zebrafish were placed in random tanks as C: Control (n=15); GP: Gingipain (n=15); OF: Overfeeding (n=15); and OF+GP: Overfeeding+Gingipain (n=15) groups. At the end of the experiment, levels of intestinal lipid peroxidation (LPO) and ALP, glutathione S-transferase (GST), and catalase (CAT) activities were evaluated.

Results: Intestinal LPO was significantly lower in the GP and OF groups compared to C. Gingipain injection in OF (OF+GP) significantly elevated LPO when compared to C, GP, and OF groups. ALP activities decreased significantly in the GP, OF, and OF+GP compared to C. GST activities increased significantly in the GP when compared to C. Decreased GST activities were observed in the OF and OF+GP. This decrease was less in OF+GP. CAT activities significantly decreased in all groups when compared to C.

Conclusion: Our findings demonstrate that gingipain injection alters the ALP activity and intestinal oxidant-antioxidant status in overfed zebrafish.

Keywords: Gingipain, zebrafish, oxidative stress

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APA

Gündüz, G., Beler, M., Unal, I., Cansız, D., Emekli Alturfan, E., & Köse, K.N. (2023). Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish. Experimed, 13(2), 80-85. https://doi.org/10.26650/experimed.1268503


AMA

Gündüz G, Beler M, Unal I, Cansız D, Emekli Alturfan E, Köse K N. Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish. Experimed. 2023;13(2):80-85. https://doi.org/10.26650/experimed.1268503


ABNT

Gündüz, G.; Beler, M.; Unal, I.; Cansız, D.; Emekli Alturfan, E.; Köse, K.N. Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish. Experimed, [Publisher Location], v. 13, n. 2, p. 80-85, 2023.


Chicago: Author-Date Style

Gündüz, Gizem, and Merih Beler and Ismail Unal and Derya Cansız and Ebru Emekli Alturfan and Kemal Naci Köse. 2023. “Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish.” Experimed 13, no. 2: 80-85. https://doi.org/10.26650/experimed.1268503


Chicago: Humanities Style

Gündüz, Gizem, and Merih Beler and Ismail Unal and Derya Cansız and Ebru Emekli Alturfan and Kemal Naci Köse. “Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish.” Experimed 13, no. 2 (Sep. 2023): 80-85. https://doi.org/10.26650/experimed.1268503


Harvard: Australian Style

Gündüz, G & Beler, M & Unal, I & Cansız, D & Emekli Alturfan, E & Köse, KN 2023, 'Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish', Experimed, vol. 13, no. 2, pp. 80-85, viewed 30 Sep. 2023, https://doi.org/10.26650/experimed.1268503


Harvard: Author-Date Style

Gündüz, G. and Beler, M. and Unal, I. and Cansız, D. and Emekli Alturfan, E. and Köse, K.N. (2023) ‘Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish’, Experimed, 13(2), pp. 80-85. https://doi.org/10.26650/experimed.1268503 (30 Sep. 2023).


MLA

Gündüz, Gizem, and Merih Beler and Ismail Unal and Derya Cansız and Ebru Emekli Alturfan and Kemal Naci Köse. “Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish.” Experimed, vol. 13, no. 2, 2023, pp. 80-85. [Database Container], https://doi.org/10.26650/experimed.1268503


Vancouver

Gündüz G, Beler M, Unal I, Cansız D, Emekli Alturfan E, Köse KN. Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish. Experimed [Internet]. 30 Sep. 2023 [cited 30 Sep. 2023];13(2):80-85. Available from: https://doi.org/10.26650/experimed.1268503 doi: 10.26650/experimed.1268503


ISNAD

Gündüz, Gizem - Beler, Merih - Unal, Ismail - Cansız, Derya - Emekli Alturfan, Ebru - Köse, KemalNaci. “Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish”. Experimed 13/2 (Sep. 2023): 80-85. https://doi.org/10.26650/experimed.1268503



TIMELINE


Submitted24.03.2023
Accepted05.05.2023
Published Online03.08.2023

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