Research Article


DOI :10.26650/experimed.1364227   IUP :10.26650/experimed.1364227    Full Text (PDF)

Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells

Ebru Hacıosmanoğlu AldoğanFulya Dal YöntemŞeyma BulutHande YapışlarBaşak GünçerMuhammet Bektaş

Objective: Inflammation is a complex reaction present in numerous disorders. Indomethacin, a compound possessing an indoline core, is a Nonsteroidal Anti-Inflammatory Drug (NSAID) that is commonly prescribed for inflammation and pain. The actin network, plays a major role in cellular activities and it’s regulated by by Rho GTPases has important implications for cellular dynamics and orientation. In this research, we explore the effects of indomethacin on the inflammatory response as mediated via RhoA and pyrin inflammatory complexes using an inflammatory disease model with relation actin cytoskeleton.

Materials and Methods: This study used Western blotting to examine the impact of indomethacin on the assembly processes related to the pyrin inflammasome complex and the RhoA signaling pathway in Lipopolysaccharide-stimulated THP-1 cells. Actin-indomethacin interaction was analyzed by Differential Scanning Fluorimetry (DSF).

Results: We found that while the expression levels of pyrin decreased, phosphorylated-RhoA increased but overall RhoA levels did not change. The equilibrium dissociation constant (KD) for the G-actin-indomethacin complex was calculated to be 9.591± 1.608 ng/mL (R2 = 0.8582) using ∆Tm measurements of indomethacin by DSF.

Conclusion: Moreover, the effects of indomethacin on inflammation pathways may provide insight into the molecular mechanisms of pyrin inflammasome formation in various autoimmune diseases.


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APA

Hacıosmanoğlu Aldoğan, E., Dal Yöntem, F., Bulut, Ş., Yapışlar, H., Günçer, B., & Bektaş, M. (2023). Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells. Experimed, 13(3), 213-217. https://doi.org/10.26650/experimed.1364227


AMA

Hacıosmanoğlu Aldoğan E, Dal Yöntem F, Bulut Ş, Yapışlar H, Günçer B, Bektaş M. Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells. Experimed. 2023;13(3):213-217. https://doi.org/10.26650/experimed.1364227


ABNT

Hacıosmanoğlu Aldoğan, E.; Dal Yöntem, F.; Bulut, Ş.; Yapışlar, H.; Günçer, B.; Bektaş, M. Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells. Experimed, [Publisher Location], v. 13, n. 3, p. 213-217, 2023.


Chicago: Author-Date Style

Hacıosmanoğlu Aldoğan, Ebru, and Fulya Dal Yöntem and Şeyma Bulut and Hande Yapışlar and Başak Günçer and Muhammet Bektaş. 2023. “Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells.” Experimed 13, no. 3: 213-217. https://doi.org/10.26650/experimed.1364227


Chicago: Humanities Style

Hacıosmanoğlu Aldoğan, Ebru, and Fulya Dal Yöntem and Şeyma Bulut and Hande Yapışlar and Başak Günçer and Muhammet Bektaş. Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells.” Experimed 13, no. 3 (Jul. 2024): 213-217. https://doi.org/10.26650/experimed.1364227


Harvard: Australian Style

Hacıosmanoğlu Aldoğan, E & Dal Yöntem, F & Bulut, Ş & Yapışlar, H & Günçer, B & Bektaş, M 2023, 'Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells', Experimed, vol. 13, no. 3, pp. 213-217, viewed 18 Jul. 2024, https://doi.org/10.26650/experimed.1364227


Harvard: Author-Date Style

Hacıosmanoğlu Aldoğan, E. and Dal Yöntem, F. and Bulut, Ş. and Yapışlar, H. and Günçer, B. and Bektaş, M. (2023) ‘Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells’, Experimed, 13(3), pp. 213-217. https://doi.org/10.26650/experimed.1364227 (18 Jul. 2024).


MLA

Hacıosmanoğlu Aldoğan, Ebru, and Fulya Dal Yöntem and Şeyma Bulut and Hande Yapışlar and Başak Günçer and Muhammet Bektaş. Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells.” Experimed, vol. 13, no. 3, 2023, pp. 213-217. [Database Container], https://doi.org/10.26650/experimed.1364227


Vancouver

Hacıosmanoğlu Aldoğan E, Dal Yöntem F, Bulut Ş, Yapışlar H, Günçer B, Bektaş M. Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells. Experimed [Internet]. 18 Jul. 2024 [cited 18 Jul. 2024];13(3):213-217. Available from: https://doi.org/10.26650/experimed.1364227 doi: 10.26650/experimed.1364227


ISNAD

Hacıosmanoğlu Aldoğan, Ebru - Dal Yöntem, Fulya - Bulut, Şeyma - Yapışlar, Hande - Günçer, Başak - Bektaş, Muhammet. Indomethacin Affects the Inflammatory Response via Interaction with the RhoA-Actin Cytoskeleton in THP-1 Cells”. Experimed 13/3 (Jul. 2024): 213-217. https://doi.org/10.26650/experimed.1364227



TIMELINE


Submitted25.09.2023
Accepted27.11.2023
Published Online28.12.2023

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