Research Article


DOI :10.26650/experimed.1343683   IUP :10.26650/experimed.1343683    Full Text (PDF)

Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation

Nursel İşçiSibel DoğanŞahika YardımSeyhun Solakoğluİlhan TahralıGünnur DenizGülnaz Nural BekiroğluElif Kervancıoğlu Demirci

Objective: This study investigated the potential synergistic effects of propolis, an antitumor and antioxidant natural product, and carboplatin, a frequently used chemotherapeutic agent for endometrial adenocarcinoma, one of the most common gynaecological cancers treatment.

Materials and Methods: Ishikawa endometrial adenocarcinoma and healthy fibroblast (3T3) cell lines were treated with 0.5 µL of carboplatin and 5 µL of propolis. Cell count, viability, migration, ultrastructure, apoptosis, and cell cycle changes were assessed using cytological and immunocytochemical methods, flow cytometry, and transmission electron microscopy (TEM).

Results: Propolis and carboplatin exhibited cytotoxic effects on Ishikawa cells. The combination of the two agents further reduced cell viability and migration. Propolis induced apoptosis through the intrinsic pathway and arrested the cell cycle in the G1 phase. TEM analysis revealed apoptosis in Ishikawa cells treated with propolis or carboplatin, while the carboplatin+propolis combination resulted in severe cell budding, apoptosis, and vacuolization. Migrasome-like structures were only observed in the Ishikawa carboplatin group. Minimal effects were observed on 3T3 cells.

Conclusion: Propolis demonstrated cytotoxic, anti-proliferative, and proapoptotic effects on tumor cells without harming healthy cells. Its ability to prevent migrasome formation suggests it may reduce chemotherapeutic resistance. Therefore, propolis shows promise as a potential enhancer of anticancer treatments.


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APA

İşçi, N., Doğan, S., Yardım, Ş., Solakoğlu, S., Tahralı, İ., Deniz, G., Bekiroğlu, G.N., & Kervancıoğlu Demirci, E. (2023). Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation. Experimed, 13(3), 194-204. https://doi.org/10.26650/experimed.1343683


AMA

İşçi N, Doğan S, Yardım Ş, Solakoğlu S, Tahralı İ, Deniz G, Bekiroğlu G N, Kervancıoğlu Demirci E. Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation. Experimed. 2023;13(3):194-204. https://doi.org/10.26650/experimed.1343683


ABNT

İşçi, N.; Doğan, S.; Yardım, Ş.; Solakoğlu, S.; Tahralı, İ.; Deniz, G.; Bekiroğlu, G.N.; Kervancıoğlu Demirci, E. Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation. Experimed, [Publisher Location], v. 13, n. 3, p. 194-204, 2023.


Chicago: Author-Date Style

İşçi, Nursel, and Sibel Doğan and Şahika Yardım and Seyhun Solakoğlu and İlhan Tahralı and Günnur Deniz and Gülnaz Nural Bekiroğlu and Elif Kervancıoğlu Demirci. 2023. “Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation.” Experimed 13, no. 3: 194-204. https://doi.org/10.26650/experimed.1343683


Chicago: Humanities Style

İşçi, Nursel, and Sibel Doğan and Şahika Yardım and Seyhun Solakoğlu and İlhan Tahralı and Günnur Deniz and Gülnaz Nural Bekiroğlu and Elif Kervancıoğlu Demirci. Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation.” Experimed 13, no. 3 (Mar. 2024): 194-204. https://doi.org/10.26650/experimed.1343683


Harvard: Australian Style

İşçi, N & Doğan, S & Yardım, Ş & Solakoğlu, S & Tahralı, İ & Deniz, G & Bekiroğlu, GN & Kervancıoğlu Demirci, E 2023, 'Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation', Experimed, vol. 13, no. 3, pp. 194-204, viewed 3 Mar. 2024, https://doi.org/10.26650/experimed.1343683


Harvard: Author-Date Style

İşçi, N. and Doğan, S. and Yardım, Ş. and Solakoğlu, S. and Tahralı, İ. and Deniz, G. and Bekiroğlu, G.N. and Kervancıoğlu Demirci, E. (2023) ‘Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation’, Experimed, 13(3), pp. 194-204. https://doi.org/10.26650/experimed.1343683 (3 Mar. 2024).


MLA

İşçi, Nursel, and Sibel Doğan and Şahika Yardım and Seyhun Solakoğlu and İlhan Tahralı and Günnur Deniz and Gülnaz Nural Bekiroğlu and Elif Kervancıoğlu Demirci. Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation.” Experimed, vol. 13, no. 3, 2023, pp. 194-204. [Database Container], https://doi.org/10.26650/experimed.1343683


Vancouver

İşçi N, Doğan S, Yardım Ş, Solakoğlu S, Tahralı İ, Deniz G, Bekiroğlu GN, Kervancıoğlu Demirci E. Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation. Experimed [Internet]. 3 Mar. 2024 [cited 3 Mar. 2024];13(3):194-204. Available from: https://doi.org/10.26650/experimed.1343683 doi: 10.26650/experimed.1343683


ISNAD

İşçi, Nursel - Doğan, Sibel - Yardım, Şahika - Solakoğlu, Seyhun - Tahralı, İlhan - Deniz, Günnur - Bekiroğlu, GülnazNural - Kervancıoğlu Demirci, Elif. Propolis: Intrinsic Pathway-Induced Apoptosis, G1 Cell Cycle Arrest, Reduced Chemotherapeutic Resistance in Adenocarcinoma, and Healthy Cell Preservation”. Experimed 13/3 (Mar. 2024): 194-204. https://doi.org/10.26650/experimed.1343683



TIMELINE


Submitted17.08.2023
Accepted03.11.2023
Published Online04.12.2023

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