Research Article


DOI :10.26650/experimed.1374959   IUP :10.26650/experimed.1374959    Full Text (PDF)

Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer

Gozde Ulfer

Objective: The aim of the study was to quantitatively evaluate HER2/neu oncogene amplification and p53 tumor suppressor gene deletion in breast cancer with real-time polymerase chain reaction (RT-PCR).

Materials and Methods: Sections obtained from the tumor tissues of 50 patients were paraffinized on slides, and DNA extraction was performed. HER2/neu amplification and p53 deletion were analyzed using RT-PCR with respect to immunohistochemistry (IHC).

Results: For 25 patients with breast cancer, we compared IHC and RT-PCR results for the quantitative measurement of HER2/neu expression. We found a significant correlation between the results obtained using IHC and RT-PCR (p < 0.05). Taking the results of IHC as reference, the sensitivity and specificity of the RT-PCR method were 57% and 83%, respectively. HER2/neu amplification and p53 deletion did not have a significant correlation with tumor size, histological grade, lymph node invasion, and status of estrogen receptor and progesterone receptor (p>0.05, for all).

Conclusion: RT-PCR measured gene levels reliably and accurately with high sensitivity and specificity, making it superior to IHC, which is subjective.


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APA

Ulfer, G. (2023). Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer. Experimed, 13(3), 257-262. https://doi.org/10.26650/experimed.1374959


AMA

Ulfer G. Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer. Experimed. 2023;13(3):257-262. https://doi.org/10.26650/experimed.1374959


ABNT

Ulfer, G. Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer. Experimed, [Publisher Location], v. 13, n. 3, p. 257-262, 2023.


Chicago: Author-Date Style

Ulfer, Gozde,. 2023. “Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer.” Experimed 13, no. 3: 257-262. https://doi.org/10.26650/experimed.1374959


Chicago: Humanities Style

Ulfer, Gozde,. Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer.” Experimed 13, no. 3 (Jul. 2024): 257-262. https://doi.org/10.26650/experimed.1374959


Harvard: Australian Style

Ulfer, G 2023, 'Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer', Experimed, vol. 13, no. 3, pp. 257-262, viewed 24 Jul. 2024, https://doi.org/10.26650/experimed.1374959


Harvard: Author-Date Style

Ulfer, G. (2023) ‘Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer’, Experimed, 13(3), pp. 257-262. https://doi.org/10.26650/experimed.1374959 (24 Jul. 2024).


MLA

Ulfer, Gozde,. Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer.” Experimed, vol. 13, no. 3, 2023, pp. 257-262. [Database Container], https://doi.org/10.26650/experimed.1374959


Vancouver

Ulfer G. Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer. Experimed [Internet]. 24 Jul. 2024 [cited 24 Jul. 2024];13(3):257-262. Available from: https://doi.org/10.26650/experimed.1374959 doi: 10.26650/experimed.1374959


ISNAD

Ulfer, Gozde. Quantitative Measurement of HER2/neu Oncogene Amplification and p53 Tumor Suppressor Gene Deletion by RT-PCR in Breast Cancer”. Experimed 13/3 (Jul. 2024): 257-262. https://doi.org/10.26650/experimed.1374959



TIMELINE


Submitted16.10.2023
Accepted29.11.2023
Published Online28.12.2023

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