The Effect of Whey Proteins on the Brain and Small  Intestine Nitric Oxide Levels: Protein Profiles in  Methotrexate-Induced Oxidative Stress

Yılmaz Sümeyye; Tufan Elif; Göksun Sivas Güzin; Gökmen Begüm; Dursun Ercan; Özbeyli Dilek; Şener Göksel; Tunalı Akbay Tuğba

doi:https://dx.doi.org/10.26650/experimed.1189898

Research Article

DOI :10.26650/experimed.1189898 IUP :10.26650/experimed.1189898 Full Text (PDF)

The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress

Sümeyye Yılmaz, Elif Tufan, Güzin Göksun Sivas, Begüm Gürel Gökmen, Ercan Dursun, Dilek Özbeyli, Göksel Şener, Tuğba Tunalı Akbay

Objectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage. Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-S-transferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out. Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTXinduced oxidative stress. MTX administration did not change the brain’s NO level, while an increase in intestinal NO level was detected. Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage.

Keywords: Methotrexate, whey protein, brain, small intestine, nitric oxide, oxidative stress

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APA

Yılmaz, S., Tufan, E., Göksun Sivas, G., Gökmen, B.G., Dursun, E., Özbeyli, D., Şener, G., & Tunalı Akbay, T. (2022). The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress. Experimed, 12(3), 113-118. https://doi.org/10.26650/experimed.1189898

AMA

Yılmaz S, Tufan E, Göksun Sivas G, Gökmen B G, Dursun E, Özbeyli D, Şener G, Tunalı Akbay T. The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress. Experimed. 2022;12(3):113-118. https://doi.org/10.26650/experimed.1189898

ABNT

Yılmaz, S.; Tufan, E.; Göksun Sivas, G.; Gökmen, B.G.; Dursun, E.; Özbeyli, D.; Şener, G.; Tunalı Akbay, T. The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress. Experimed, [Publisher Location], v. 12, n. 3, p. 113-118, 2022.

Chicago: Author-Date Style

Yılmaz, Sümeyye, and Elif Tufan and Güzin Göksun Sivas and Begüm Gürel Gökmen and Ercan Dursun and Dilek Özbeyli and Göksel Şener and Tuğba Tunalı Akbay. 2022. “The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress.” Experimed 12, no. 3: 113-118. https://doi.org/10.26650/experimed.1189898

Chicago: Humanities Style

Yılmaz, Sümeyye, and Elif Tufan and Güzin Göksun Sivas and Begüm Gürel Gökmen and Ercan Dursun and Dilek Özbeyli and Göksel Şener and Tuğba Tunalı Akbay. “The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress.” Experimed 12, no. 3 (Sep. 2024): 113-118. https://doi.org/10.26650/experimed.1189898

Harvard: Australian Style

Yılmaz, S & Tufan, E & Göksun Sivas, G & Gökmen, BG & Dursun, E & Özbeyli, D & Şener, G & Tunalı Akbay, T 2022, 'The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress', Experimed, vol. 12, no. 3, pp. 113-118, viewed 9 Sep. 2024, https://doi.org/10.26650/experimed.1189898

Harvard: Author-Date Style

Yılmaz, S. and Tufan, E. and Göksun Sivas, G. and Gökmen, B.G. and Dursun, E. and Özbeyli, D. and Şener, G. and Tunalı Akbay, T. (2022) ‘The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress’, Experimed, 12(3), pp. 113-118. https://doi.org/10.26650/experimed.1189898 (9 Sep. 2024).

MLA

Yılmaz, Sümeyye, and Elif Tufan and Güzin Göksun Sivas and Begüm Gürel Gökmen and Ercan Dursun and Dilek Özbeyli and Göksel Şener and Tuğba Tunalı Akbay. “The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress.” Experimed, vol. 12, no. 3, 2022, pp. 113-118. [Database Container], https://doi.org/10.26650/experimed.1189898

Vancouver

Yılmaz S, Tufan E, Göksun Sivas G, Gökmen BG, Dursun E, Özbeyli D, Şener G, Tunalı Akbay T. The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress. Experimed [Internet]. 9 Sep. 2024 [cited 9 Sep. 2024];12(3):113-118. Available from: https://doi.org/10.26650/experimed.1189898 doi: 10.26650/experimed.1189898

ISNAD

Yılmaz, Sümeyye - Tufan, Elif - Göksun Sivas, Güzin - Gökmen, BegümGürel - Dursun, Ercan - Özbeyli, Dilek - Şener, Göksel - Tunalı Akbay, Tuğba. “The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress”. Experimed 12/3 (Sep. 2024): 113-118. https://doi.org/10.26650/experimed.1189898

Volume 12, Issue 32022, P. 113-118

TIMELINE

Submitted	16.10.2022
Accepted	16.11.2022
Published Online	27.12.2022

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