Research Article


DOI :10.26650/IstanbulJPharm.2022.1094112   IUP :10.26650/IstanbulJPharm.2022.1094112    Full Text (PDF)

Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines

İrem AtlıhanSevgi Koçyiğit SevinçOya OrunÖzgür YılmazŞükriye Güniz KüçükgüzelPınar Mega Tiber

Background and Aims: The incidence of breast cancer is increasing day by day, especially in women. The search for new drugs against breast cancer is the focus of attention in research. Breast cancer and prostate cancer have remarkable biological similarities. Therefore, the 4-(4-chlorophenyl)-3-(1-(2-fluoro-[1,1'-biphenyl]-4-yl)ethyl)-5-((4-fluorobenzyl)thio)-4H-1,2,4-triazole (SGK597) compound that is suppressing cell proliferation in prostate cancer, was studied in MCF-7 breast cancer and MCF-10A mammary epithelial cell lines. Methods: The WST-8 method was used to determine cell viability and cytotoxicity of SGK597 in MCF-7 and MCF10-A cell lines. The JC-1 test was applied to determine changes in mitochondrial membrane potential. The protein expression levels of Bax, Bcl-2, and c-PARP associated with apoptosis were determined using Western blot analysis. Results: After 24 and 48 hours of incubation of SGK597, the IC50 values were 28.74 μM and 17.28 μM for MCF-7; 65.9 μM and 50.5 μM for MCF-10A, respectively. Mitochondrial membrane potential showed a tendency toward depolarization in MCF-7 cells as a result of increasing concentration of SGK597, while the same tendency was not seen for MCF-10A. As a result of western blot experiments, no increase in the Bax/Bcl-2 ratio and c-PARP expression level was observed, indicating no apoptosis. Conclusion: It was observed that the compound SGK597 suppressed MCF-7 cell proliferation. These results indicate that SGK597 may be a candidate compound for use as an anticancer agent.


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APA

Atlıhan, İ., Koçyiğit Sevinç, S., Orun, O., Yılmaz, Ö., Güniz Küçükgüzel, Ş., & Mega Tiber, P. (2022). Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines. İstanbul Journal of Pharmacy, 52(3), 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112


AMA

Atlıhan İ, Koçyiğit Sevinç S, Orun O, Yılmaz Ö, Güniz Küçükgüzel Ş, Mega Tiber P. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines. İstanbul Journal of Pharmacy. 2022;52(3):265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112


ABNT

Atlıhan, İ.; Koçyiğit Sevinç, S.; Orun, O.; Yılmaz, Ö.; Güniz Küçükgüzel, Ş.; Mega Tiber, P. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines. İstanbul Journal of Pharmacy, [Publisher Location], v. 52, n. 3, p. 265-270, 2022.


Chicago: Author-Date Style

Atlıhan, İrem, and Sevgi Koçyiğit Sevinç and Oya Orun and Özgür Yılmaz and Şükriye Güniz Küçükgüzel and Pınar Mega Tiber. 2022. “Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines.” İstanbul Journal of Pharmacy 52, no. 3: 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112


Chicago: Humanities Style

Atlıhan, İrem, and Sevgi Koçyiğit Sevinç and Oya Orun and Özgür Yılmaz and Şükriye Güniz Küçükgüzel and Pınar Mega Tiber. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines.” İstanbul Journal of Pharmacy 52, no. 3 (Feb. 2023): 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112


Harvard: Australian Style

Atlıhan, İ & Koçyiğit Sevinç, S & Orun, O & Yılmaz, Ö & Güniz Küçükgüzel, Ş & Mega Tiber, P 2022, 'Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines', İstanbul Journal of Pharmacy, vol. 52, no. 3, pp. 265-270, viewed 1 Feb. 2023, https://doi.org/10.26650/IstanbulJPharm.2022.1094112


Harvard: Author-Date Style

Atlıhan, İ. and Koçyiğit Sevinç, S. and Orun, O. and Yılmaz, Ö. and Güniz Küçükgüzel, Ş. and Mega Tiber, P. (2022) ‘Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines’, İstanbul Journal of Pharmacy, 52(3), pp. 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112 (1 Feb. 2023).


MLA

Atlıhan, İrem, and Sevgi Koçyiğit Sevinç and Oya Orun and Özgür Yılmaz and Şükriye Güniz Küçükgüzel and Pınar Mega Tiber. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines.” İstanbul Journal of Pharmacy, vol. 52, no. 3, 2022, pp. 265-270. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2022.1094112


Vancouver

Atlıhan İ, Koçyiğit Sevinç S, Orun O, Yılmaz Ö, Güniz Küçükgüzel Ş, Mega Tiber P. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines. İstanbul Journal of Pharmacy [Internet]. 1 Feb. 2023 [cited 1 Feb. 2023];52(3):265-270. Available from: https://doi.org/10.26650/IstanbulJPharm.2022.1094112 doi: 10.26650/IstanbulJPharm.2022.1094112


ISNAD

Atlıhan, İrem - Koçyiğit Sevinç, Sevgi - Orun, Oya - Yılmaz, Özgür - Güniz Küçükgüzel, Şükriye - Mega Tiber, Pınar. Effect of flurbiprofen derivative (SGK597) on cell proliferation and apoptosis of breast cancer cell lines”. İstanbul Journal of Pharmacy 52/3 (Feb. 2023): 265-270. https://doi.org/10.26650/IstanbulJPharm.2022.1094112



TIMELINE


Submitted30.03.2022
Accepted22.09.2022
Published Online30.12.2022

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