İstanbul Journal of Pharmacy

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DOI :10.26650/IstanbulJPharm.2023.1024145   IUP :10.26650/IstanbulJPharm.2023.1024145    Full Text (PDF)

Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species

Mehmet Akdeniz, İsmail Yener, Şafak Özhan Kocakaya, Murat Yolcu, Serkan Yiğitkan, Fırat Aydın, Fatma Pınar Türkmenoğlu, Abdulselam Ertaş

Background and Aims: The importance of Hypericum species that are used traditionally against many diseases is increasing day by day.

Methods: In this study, the essential oil contents of Hypericum triquetrifolium, H. empetrifolium subsp. empetrifolium, and H. pruinatum species were determined with GC-MS/FID. This is the first study on the antioxidant, anticholinesterase, antiurease, antityrosinase, antielastase, and anticollagenase activities of these species. Also, in silico and in vitro enzyme inhibitory activities of the major compounds in the essential oil samples of the species have been evaluated. In addition, the cytotoxic effects of the essential oils were determined by the MTT method.

Results: According to GC-MS/FID results, the major compounds were determined as caryophyllene oxide (16.76%) for H. triquetrifolium, α-pinene (21.67%) for H. empetrifolium subsp. empetrifolium, and germacrene D (22.47%) for H. pruinatum. Especially, H. pruinatum sample showed a high cytotoxic effect (IC50: 34.78±0.22 and 29.06±0.40 µg/mL, respectively) on HT29 and MCF-7 cell lines. It was determined that the same sample showed a promising inhibitory activity on acetyl (18.33±2.79, 36.48±2.40, and 56.97±0.94, respectively) and butyryl (71.63±2.78, 73.88±1.16, and 56.97±0.97, respectively) cholinesterase enzymes.

Conclusion: Results of the in-vitro activity studies indicated that H. pruinatum essential oil could be used in the pharmaceutical industry.

Keywords: Hypericum triquetrifolium, H. empetrifolium, H. pruinatum, essential oil, cytotoxicity, anti-aging, in silico

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APA

Akdeniz, M., Yener, İ., Kocakaya, Ş.Ö., Yolcu, M., Yiğitkan, S., Aydın, F., Türkmenoğlu, F.P., & Ertaş, A. (2023). Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species. İstanbul Journal of Pharmacy, 53(2), 177-185. https://doi.org/10.26650/IstanbulJPharm.2023.1024145


AMA

Akdeniz M, Yener İ, Kocakaya Ş Ö, Yolcu M, Yiğitkan S, Aydın F, Türkmenoğlu F P, Ertaş A. Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species. İstanbul Journal of Pharmacy. 2023;53(2):177-185. https://doi.org/10.26650/IstanbulJPharm.2023.1024145


ABNT

Akdeniz, M.; Yener, İ.; Kocakaya, Ş.Ö.; Yolcu, M.; Yiğitkan, S.; Aydın, F.; Türkmenoğlu, F.P.; Ertaş, A. Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species. İstanbul Journal of Pharmacy, [Publisher Location], v. 53, n. 2, p. 177-185, 2023.


Chicago: Author-Date Style

Akdeniz, Mehmet, and İsmail Yener and Şafak Özhan Kocakaya and Murat Yolcu and Serkan Yiğitkan and Fırat Aydın and Fatma Pınar Türkmenoğlu and Abdulselam Ertaş. 2023. “Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species.” İstanbul Journal of Pharmacy 53, no. 2: 177-185. https://doi.org/10.26650/IstanbulJPharm.2023.1024145


Chicago: Humanities Style

Akdeniz, Mehmet, and İsmail Yener and Şafak Özhan Kocakaya and Murat Yolcu and Serkan Yiğitkan and Fırat Aydın and Fatma Pınar Türkmenoğlu and Abdulselam Ertaş. “Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species.” İstanbul Journal of Pharmacy 53, no. 2 (Dec. 2023): 177-185. https://doi.org/10.26650/IstanbulJPharm.2023.1024145


Harvard: Australian Style

Akdeniz, M & Yener, İ & Kocakaya, ŞÖ & Yolcu, M & Yiğitkan, S & Aydın, F & Türkmenoğlu, FP & Ertaş, A 2023, 'Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species', İstanbul Journal of Pharmacy, vol. 53, no. 2, pp. 177-185, viewed 9 Dec. 2023, https://doi.org/10.26650/IstanbulJPharm.2023.1024145


Harvard: Author-Date Style

Akdeniz, M. and Yener, İ. and Kocakaya, Ş.Ö. and Yolcu, M. and Yiğitkan, S. and Aydın, F. and Türkmenoğlu, F.P. and Ertaş, A. (2023) ‘Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species’, İstanbul Journal of Pharmacy, 53(2), pp. 177-185. https://doi.org/10.26650/IstanbulJPharm.2023.1024145 (9 Dec. 2023).


MLA

Akdeniz, Mehmet, and İsmail Yener and Şafak Özhan Kocakaya and Murat Yolcu and Serkan Yiğitkan and Fırat Aydın and Fatma Pınar Türkmenoğlu and Abdulselam Ertaş. “Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species.” İstanbul Journal of Pharmacy, vol. 53, no. 2, 2023, pp. 177-185. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2023.1024145


Vancouver

Akdeniz M, Yener İ, Kocakaya ŞÖ, Yolcu M, Yiğitkan S, Aydın F, Türkmenoğlu FP, Ertaş A. Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species. İstanbul Journal of Pharmacy [Internet]. 9 Dec. 2023 [cited 9 Dec. 2023];53(2):177-185. Available from: https://doi.org/10.26650/IstanbulJPharm.2023.1024145 doi: 10.26650/IstanbulJPharm.2023.1024145


ISNAD

Akdeniz, Mehmet - Yener, İsmail - Kocakaya, ŞafakÖzhan - Yolcu, Murat - Yiğitkan, Serkan - Aydın, Fırat - Türkmenoğlu, FatmaPınar - Ertaş, Abdulselam. “Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species”. İstanbul Journal of Pharmacy 53/2 (Dec. 2023): 177-185. https://doi.org/10.26650/IstanbulJPharm.2023.1024145



TIMELINE


Submitted17.11.2021
Accepted28.12.2022
Published Online28.08.2023

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