Research Article


DOI :10.26650/IstanbulJPharm.2020.0044   IUP :10.26650/IstanbulJPharm.2020.0044    Full Text (PDF)

The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study

Robert TungadiWidysusanti AbdulkadirMunafri Tahir

Background and Aims: The study was conducted to determine the optimal concentration of water-soluble snakehead fish dry extract (SFDE) in nanoemulsion and the amount of albumin required to penetrate the skin in order to accelerate the wound healing process. Methods: The snakehead fish (SF) was extracted using an atomizer while the nanoemulsion basis was optimized using oleic acid, Tween 80, and propylene glycol. The developed SFDE in nanoemulsion was characterized based on droplet size, PDI, and zeta potential. The ability of the mixture to penetrate the snakeskin was tested using Franz diffusion cells. The effectiveness of the nanoemulsion was evaluated by dividing the rabbits used for experiment into 6 treatment groups including SFDE F1 0.25%, F2 0.5%, F3 1%, F4 SF 2% cream, F5 nanoemulsion basis, and F6 no treatment. Results: The SFDE nanoemulsion produced a particle size of 147.5 nm with acceptable PDI (0.23) and zeta potential (+13.38 mV). The most effective SFDE to accelerate the healing of open wounds in rabbits was a concentration of 1%, which was found to have dried and closed the wound on the 3rd day. Conclusion: The permeation study and the effectiveness test showed the 1% SFDE nanoemulsion is the best concentration in accelerating the wound healing process and ensuring the highest albumin penetration into the skin.


PDF View

References

  • Alves, M. P., Scarrone, A. L., Santos, M., Pohlmann, A. R., & Guterren S. S. (2007). Human skin penetration and distribution of nimesu-lide from hydrophilic gels. International Journal of Pharmaceutics, 314(1-2), 215-220. google scholar
  • Barot, B. S., Parejiya, P. B., Patel, H. K, Mehta D. M., & Shelat, P. K. (2012). Microemulsion-based antifungal gel delivery to nail for the treat-ment of onychomycosis: Formulation, optimization, and efficacy studies. Drug Delivery and Translational Research, 2(6), 463-476. google scholar
  • Bernard, P. B. (2012). Modern Aspects of Emulsion Science, Emulsions-Recent Advances in Understanding. UK: Royal Science of Chemistry. google scholar
  • Brandelero, R. P. H., Yamashita, F., & Grossmann, M. V. E. (2010). The effect of surfactant Tween 80 on the hydrophilic water vapor permeation, and the mechanical properties of cassava starch and poly (butylenes adipate-co-terephtalate) (pbat) blend films. Car-bohydrate Polymers, 82, 1102-1109. google scholar
  • Ceve, G. (2004). Lipid vesicles and other colloids as drug carriers on the skin. Advanced Drug Delivery Review, 56(5), 675-711. google scholar
  • Chuesiang, P., Siripatrawan, U., Sanguandeekul, R., McLandsbor-ough, L., & McClements, D. J. (2018). Optimization of cinnamon oil nanoemulsions using phase inversion temperature method: Impact of oil phase composition and surfactant concentration. Journal of Colloid and Interface Science, 514, 208-216. google scholar
  • Devarajan, V., & Ravichandran, V. (2011). Nanoemulsions: As modi-fied drug delivery tool. International Journal of Comprehensive Pharmacy, 2, 1-5. google scholar
  • Hadian, Z., Sahari, M. A., & Moghimi, H. R. (2014). Formulation, characterization and optimization of liposomes containing EPA and DHA; A methodology approach. Iranian Journal of Pharma-ceutical Research, 13(2), 393-404. google scholar
  • Kong, M., Chen, X. G., Kweon, D. K., & Park, H. J. (2011). Investiga-tion on skin hyaluronic acid based on nanoemulsion as transder-mal carrier. Carbohydrate Polymers, 86(2), 837-843. google scholar
  • Kumar, M., Bishnoi, R. S., Shukla, A. K., & Jain, P. (2019). Techniques for formulation of nanoemulsion drug delivery system: A review. Preventive Nutrition and Food Science, 24(3), 225-234. google scholar
  • Lala, R., & Awari, N. (2014). Nanoemulsion-based gel formulations of COX-2 inhibitors for enhanced efficacy in inflammatory condi-tions. Applied Nanoscience, 4, 143-151. google scholar
  • Laxmi, M., Bhardwaj, A., Mehta, S., & Mehta, A. (2015). Develop-ment and characterization of nanoemulsion as carrier for the en-hancement of bioavailability of artemether. Artificial Cells Nano-medicine and Biotechnology, 43(5), 334-344. google scholar
  • Lovelyn, C., & Attama, A. A. (2011). Current state of nanoemulsions in drug delivery. Journal of Biomaterials and Nanobiotechnology, 2(5), 626-639. google scholar
  • Madene, A., Jacquot, M., Scher, J., & Desobry, S. (2006). Flavour encapsulation and controlled release - a review. International Journal of Food Science and Technology, 41, 1-21. google scholar
  • Mansyur. (2010, July 15). Analysis of snakehead fish dry extract. Indonesian Institute of Sciences Biotechnology Research Cen-ter. Retrieved from https://worldwidescience.org/topicpages/s/ snakehead+fish+channa.html google scholar
  • Maryanto, A. (2004, June 18). The impact of albumin serum on length of postoperative wound healing process, Faculty of Medi-cine, University of Gadjah Mada. Retrieved from http://etd.reposi-tory.ugm.ac.id/home/detail_pencarian/25247 google scholar
  • Mou, D., Chen, H., Du, D., Mao, C., Wan, J., Xu, H., & Yang, X. (2008). Hy-drogel thickened nanoemulsion system for topical delivery of lipo-philic drugs. International Journal of Pharmaceutics, 353(1-2), 270-276. google scholar
  • Mustafa, A., Widodo, A., & Kristianto, Y. (2012). Albumin and zinc content of snakehead fish extract and its role in health. Interna-tional. Journal of Science and Technology, 1, 1-8. google scholar
  • Natalia, M. (2012). The stability and antibacterial activity test of black cumin oil (nigella sativa l.) nano-emulsion gel (nano-emulgel). (Master’s thesis). Retrieved from http://lib.ui.ac.id/ file?file=digital/20309121-S43091-Uji%20stabilitas.pdf google scholar
  • Ngawhirunpat, T., Panomsuk, S., Opanasopit, P., Rojanata, T., & Hatanaka, T. (2006). Comparison of the percutaneous absorption of hydrophilic and lipophilic compounds in shed snake skin and human skin. Pharmazie, 61(4), 331-335. google scholar
  • Rehman, F. U., Shah, K. U., Shah, S. U., Khan, I. U., Khan, G. M., & Khan, A. (2017). From nanoemulsions to self-nanoemulsions, with recent advances in self-nanoemulsifying drug delivery systems (SNEDDS). Expert Opinion on Drug Delivery, 14(11), 1325-1340. google scholar
  • Shah, P., Bhalodia, D., & Shelat, P. (2011). Nanoemulsion : A phar-maceutical review. Systematic Reviews in Pharmacy, 1, 24-32. google scholar
  • Singh, R., & Lillard, J. W. (2014). Nanoparticle-based targeted drug delivery. Experimental Molecular and Pathology, 86(3), 215-223. google scholar
  • Suprayitno, E. (2003). Snakehead Fish (Ophiocephalus striatus) al-bumin as functional food to overcome future nutrition problems. Faculty of Fisheries, Brawijaya University, 5(3), 32-36. google scholar
  • Swarbrick, J. (2007). Encyclopedia of pharmaceutical technology. New York: Informa Healthcare USA Press, pp 1548-1565. google scholar
  • Tungadi, R. (2011). The acceleration of wound healing of snake-head fish cream towards rabbit’s skin wound histopathologically. Indonesian Pharmaceutical Journal, 9, 91-97. google scholar
  • Tungadi, R., & Hasan, A. M. (2016). The effect of penetrant en-hancer combination towards the diffusion rate of snakehead fish (Ophiocephalus striatus) cream in vitro and vivo. International Journal of PharmTech Research, 9(6), 508-13. google scholar
  • Tungadi, R., Moo, D. R., & Mozin, W. R. (2017). Characterization and physical stability evaluation of snakehead fish (Ophiocephalus striatus) powder nanoemulsion. International Journal of Pharma-ceutical Sciences and Research, 8(6), 2720-4. google scholar
  • Tungadi, R., Susanty, W., Wicita, P., & Pido, E. (2018). Transdermal delivery of snakehead fish (Ophiocephalus striatus) nanoemulgel containing hydrophobic powder for burn wound. Pharmaceutical Sciences, 24(4), 313-323. google scholar
  • Tungadi, R. (2019). Potential of snakehead fish (Ophiocephalus striatus) in accelerating wound healing. Universal Journal of Phar-maceutical Research, 4(5), 40-44. google scholar
  • Tungadi, R., Abdulkadir, W., Ischak, N. I., & Rahim, B. R. (2019). Li-posomal formulation of snakehead fish (Ophiocephalus striatus) powder and toxicity study in zebrafish (Danio rerio) model. Phar-maceutical Sciences, 25(2), 145-153. google scholar
  • Tungadi, R., & Wicita, P. (2020). Formulation, optimization, and char-acterization of snakehead fish (Ophiocephalus striatus) powder nanoemulgel. Brazilian Journal of Pharmaceutical Sciences, 56, 1-8. Ulandari, A., Kurniawan, D., & Putri, A. S. (2011). Potential of snake-head fish protein in preventing kwashiorkor in toddlers in Jambi Province. Faculty of Medicine, Jambi University. Retrieved from https://adoc.pub/potensi-protein-ikan-gabus-dalam-mence-gah-kwashiorkor-pada-b.html google scholar
  • Vemula, V. R., Lagishetty, V., & Lingala, S. (2010). Solubility en-hancement techniques. International Journal of Pharmaceutical Science Review and Research, 5(1), 41-51. google scholar
  • Winterhalter, M., & Lasic, D. D. (2013). Liposome stability and for-mation: experimental parameters and theories on the size distri-bution. Chemistry and Physics of Lipids, 64, 35-37. google scholar
  • Zhang, L., Zhang, F., Fan, Z., Liu, B., & Meng, X. (2019). DHA and EPA nanoemulsion prepared by the low-energy emulsification method: process factors influencing droplet size physicochemi-cal stability. Food Research International, 121(7), 359-366. google scholar

Citations

Copy and paste a formatted citation or use one of the options to export in your chosen format


EXPORT



APA

Tungadi, R., Abdulkadir, W., & Tahir, M. (2021). The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. İstanbul Journal of Pharmacy, 51(1), 35-41. https://doi.org/10.26650/IstanbulJPharm.2020.0044


AMA

Tungadi R, Abdulkadir W, Tahir M. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. İstanbul Journal of Pharmacy. 2021;51(1):35-41. https://doi.org/10.26650/IstanbulJPharm.2020.0044


ABNT

Tungadi, R.; Abdulkadir, W.; Tahir, M. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. İstanbul Journal of Pharmacy, [Publisher Location], v. 51, n. 1, p. 35-41, 2021.


Chicago: Author-Date Style

Tungadi, Robert, and Widysusanti Abdulkadir and Munafri Tahir. 2021. “The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study.” İstanbul Journal of Pharmacy 51, no. 1: 35-41. https://doi.org/10.26650/IstanbulJPharm.2020.0044


Chicago: Humanities Style

Tungadi, Robert, and Widysusanti Abdulkadir and Munafri Tahir. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study.” İstanbul Journal of Pharmacy 51, no. 1 (Jun. 2021): 35-41. https://doi.org/10.26650/IstanbulJPharm.2020.0044


Harvard: Australian Style

Tungadi, R & Abdulkadir, W & Tahir, M 2021, 'The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study', İstanbul Journal of Pharmacy, vol. 51, no. 1, pp. 35-41, viewed 23 Jun. 2021, https://doi.org/10.26650/IstanbulJPharm.2020.0044


Harvard: Author-Date Style

Tungadi, R. and Abdulkadir, W. and Tahir, M. (2021) ‘The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study’, İstanbul Journal of Pharmacy, 51(1), pp. 35-41. https://doi.org/10.26650/IstanbulJPharm.2020.0044 (23 Jun. 2021).


MLA

Tungadi, Robert, and Widysusanti Abdulkadir and Munafri Tahir. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study.” İstanbul Journal of Pharmacy, vol. 51, no. 1, 2021, pp. 35-41. [Database Container], https://doi.org/10.26650/IstanbulJPharm.2020.0044


Vancouver

Tungadi R, Abdulkadir W, Tahir M. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study. İstanbul Journal of Pharmacy [Internet]. 23 Jun. 2021 [cited 23 Jun. 2021];51(1):35-41. Available from: https://doi.org/10.26650/IstanbulJPharm.2020.0044 doi: 10.26650/IstanbulJPharm.2020.0044


ISNAD

Tungadi, Robert - Abdulkadir, Widysusanti - Tahir, Munafri. The formulation and characterization of water-soluble snakehead fish (Ophiocephalus striatus) dry extract in nanoemulsion using permeation and in vivo study”. İstanbul Journal of Pharmacy 51/1 (Jun. 2021): 35-41. https://doi.org/10.26650/IstanbulJPharm.2020.0044



TIMELINE


Submitted10.05.2020
Accepted21.09.2020
Published Online30.04.2021

LICENCE


Attribution-NonCommercial (CC BY-NC)

This license lets others remix, tweak, and build upon your work non-commercially, and although their new works must also acknowledge you and be non-commercial, they don’t have to license their derivative works on the same terms.


SHARE




Istanbul University Press aims to contribute to the dissemination of ever growing scientific knowledge through publication of high quality scientific journals and books in accordance with the international publishing standards and ethics. Istanbul University Press follows an open access, non-commercial, scholarly publishing.