Journal of Advanced Research in Health Sciences

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Research Article


DOI :10.26650/JARHS2024-1496234   IUP :10.26650/JARHS2024-1496234    Full Text (PDF)

INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR

Burak Karacan, Özden Büyükbaba Boral, Gülay İmadoğlu Yetkin, Aslı Çifcibaşı Örmeci, Ozan Özkaya, Murat Sütçü, Muradiye Acar, Bülent Ediz

Objective: This study aimed to contribute to surveillance data by determining the prevalence of pathogenic intestinal protozoans in patients with gastrointestinal complaints and to comparatively evaluate the performance of microscopy and multiplex real-time PCR methods.

Materials and Methods: Forty adults (18 years or older) and 40 children (aged 5 to 12 years) who volunteered patients with at least one gastrointestinal complaint were included in the study. Stool samples collected three times every other day from each patient were examined under a light microscope using Native-Lugol, modified formol-ether concentration, Wheatley’s modified trichrome staining, and Modified Ziehl-Neelsen staining techniques. In addition, the first stool samples were tested using multiplex real-time PCR with the Allplex™ GI Parasite Assay panel after DNA extraction. Differences between the sensitivities and specificities between the methods were analysed using Fisher’s exact test. A p-value of <0.05 was considered statistically significant.

Results: The prevalence of pathogenic intestinal protozoans was 25% in adults and 35% in children. Differences in the sensitivities and specificities of the methods were not found to be statistically significant (p=0.999).

Conclusion: Multiplex real-time PCR method performed using the Allplex™ GI Parasite Assay panel was effective even when only the first sample tested, suggesting that this method would be used for the routine diagnosis of pathogenic intestinal protozoans.

Keywords: Pathogen, intestinal, protozoon, prevalence, microscopy, PCR
DOI :10.26650/JARHS2024-1496234   IUP :10.26650/JARHS2024-1496234    Full Text (PDF)

GASTROİNTESTİNAL ŞİKÂYETİ OLAN HASTALARDA BAĞIRSAK PATOJENİ PROTOZOONLARIN PREVALANSININ MİKROSKOPİ VE MULTİPLEKS REAL-TIME PCR İLE ARAŞTIRILMASI

Burak Karacan, Özden Büyükbaba Boral, Gülay İmadoğlu Yetkin, Aslı Çifcibaşı Örmeci, Ozan Özkaya, Murat Sütçü, Muradiye Acar, Bülent Ediz

Amaç: Bu çalışmanın amacı, hem gastrointestinal şikâyeti olan hastalarda bağırsak patojeni protozoonların prevalansını tespit ederek sürveyans verilerine katkı sağlamak hem de mikroskopi ve multipleks real-time PCR yöntemlerinin performanslarını karşılaştırmalı olarak değerlendirmektir.

Gereç ve Yöntem: Çalışmaya, en az bir gastrointestinal şikâyeti olan 40 yetişkin (18 yaş ve üzerinde) ve 40 çocuk (5-12 yaş arasında) gönüllü hasta dâhil edildi. Her hastadan günaşırı olarak 3 kez alınan gaita örnekleri; Nativ-Lugol, modifiye formol-eter çöktürme, Wheatley’in trikrom boyama ve modifiye Ziehl-Neelsen boyama teknikleri uygulandıktan sonra ışık mikroskobunda incelendi. Ayrıca, yalnızca ilk olarak alınan gaita örnekleri; DNA ekstraksiyonundan sonra, Allplex™ GI Parasite Assay paneli kullanılarak multipleks real-time PCR yöntemiyle test edildi. Yöntemlerin duyarlılıkları ve özgüllükleri arasındaki farklılıklar, Fisher’in kesin testi ile analiz edildi. p değerinin <0,05 olması, istatistiksel olarak anlamlı kabul edildi.

Bulgular: Bağırsak patojeni protozoonların prevalansının yetişkin grubunda %25, çocuk grubunda ise %35 olduğu tespit edildi. Yöntemlerin duyarlılıkları ve özgüllükleri arasındaki farklılıklar, istatistiksel olarak anlamlı bulunmadı (p=0,999).

Sonuç: Allplex™ GI Parasite Assay paneli kullanılarak gerçekleştirilen multipleks real-time PCR yönteminin yalnızca ilk örnek test edildiğinde bile etkili olması; bu yöntemin, bağırsak patojeni protozoonların rutin tanısında kullanılabileceğini düşündürmektedir.

Keywords: Patojen, bağırsak, protozoon, prevalans, mikroskopi, PCR

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APA

Karacan, B., Büyükbaba Boral, Ö., İmadoğlu Yetkin, G., Çifcibaşı Örmeci, A., Özkaya, O., Sütçü, M., Acar, M., & Ediz, B. (2024). INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR. Journal of Advanced Research in Health Sciences, 7(3), 180-184. https://doi.org/10.26650/JARHS2024-1496234


AMA

Karacan B, Büyükbaba Boral Ö, İmadoğlu Yetkin G, Çifcibaşı Örmeci A, Özkaya O, Sütçü M, Acar M, Ediz B. INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR. Journal of Advanced Research in Health Sciences. 2024;7(3):180-184. https://doi.org/10.26650/JARHS2024-1496234


ABNT

Karacan, B.; Büyükbaba Boral, Ö.; İmadoğlu Yetkin, G.; Çifcibaşı Örmeci, A.; Özkaya, O.; Sütçü, M.; Acar, M.; Ediz, B. INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR. Journal of Advanced Research in Health Sciences, [Publisher Location], v. 7, n. 3, p. 180-184, 2024.


Chicago: Author-Date Style

Karacan, Burak, and Özden Büyükbaba Boral and Gülay İmadoğlu Yetkin and Aslı Çifcibaşı Örmeci and Ozan Özkaya and Murat Sütçü and Muradiye Acar and Bülent Ediz. 2024. “INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR.” Journal of Advanced Research in Health Sciences 7, no. 3: 180-184. https://doi.org/10.26650/JARHS2024-1496234


Chicago: Humanities Style

Karacan, Burak, and Özden Büyükbaba Boral and Gülay İmadoğlu Yetkin and Aslı Çifcibaşı Örmeci and Ozan Özkaya and Murat Sütçü and Muradiye Acar and Bülent Ediz. “INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR.” Journal of Advanced Research in Health Sciences 7, no. 3 (Nov. 2024): 180-184. https://doi.org/10.26650/JARHS2024-1496234


Harvard: Australian Style

Karacan, B & Büyükbaba Boral, Ö & İmadoğlu Yetkin, G & Çifcibaşı Örmeci, A & Özkaya, O & Sütçü, M & Acar, M & Ediz, B 2024, 'INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR', Journal of Advanced Research in Health Sciences, vol. 7, no. 3, pp. 180-184, viewed 15 Nov. 2024, https://doi.org/10.26650/JARHS2024-1496234


Harvard: Author-Date Style

Karacan, B. and Büyükbaba Boral, Ö. and İmadoğlu Yetkin, G. and Çifcibaşı Örmeci, A. and Özkaya, O. and Sütçü, M. and Acar, M. and Ediz, B. (2024) ‘INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR’, Journal of Advanced Research in Health Sciences, 7(3), pp. 180-184. https://doi.org/10.26650/JARHS2024-1496234 (15 Nov. 2024).


MLA

Karacan, Burak, and Özden Büyükbaba Boral and Gülay İmadoğlu Yetkin and Aslı Çifcibaşı Örmeci and Ozan Özkaya and Murat Sütçü and Muradiye Acar and Bülent Ediz. “INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR.” Journal of Advanced Research in Health Sciences, vol. 7, no. 3, 2024, pp. 180-184. [Database Container], https://doi.org/10.26650/JARHS2024-1496234


Vancouver

Karacan B, Büyükbaba Boral Ö, İmadoğlu Yetkin G, Çifcibaşı Örmeci A, Özkaya O, Sütçü M, Acar M, Ediz B. INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR. Journal of Advanced Research in Health Sciences [Internet]. 15 Nov. 2024 [cited 15 Nov. 2024];7(3):180-184. Available from: https://doi.org/10.26650/JARHS2024-1496234 doi: 10.26650/JARHS2024-1496234


ISNAD

Karacan, Burak - Büyükbaba Boral, Özden - İmadoğlu Yetkin, Gülay - Çifcibaşı Örmeci, Aslı - Özkaya, Ozan - Sütçü, Murat - Acar, Muradiye - Ediz, Bülent. “INVESTIGATION OF THE PREVALENCE OF PATHOGENIC INTESTINAL PROTOZOANS IN PATIENTS WITH GASTROINTESTINAL COMPLAINTS VIA MICROSCOPY AND MULTIPLEX REAL-TIME PCR”. Journal of Advanced Research in Health Sciences 7/3 (Nov. 2024): 180-184. https://doi.org/10.26650/JARHS2024-1496234



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Submitted05.06.2024
Published Online23.10.2024

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