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DOI :10.26650/EurJBiol.2019.0020   IUP :10.26650/EurJBiol.2019.0020    Tam Metin (PDF)

Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells

Ayşegül Yıldız

Objective: Targeted cancer therapy using targeted cell proliferation inhibitors has become increasingly more critical. Studies conducted over the last decade have shown that non-steroidal drugs containing salicylic acid (SA) such as aspirin reduce mortality in many cancers. From this perspective, there are data suggesting SA as a potential inhibitor of the mitogenic MEK1/2 (mitogen-activated-protein-kinase, MAPK), extracellular-signal regulated-protein-kinase (ERK)) signaling, which could be highly effective in the prevention of proliferation in cancer. To date, no study has been conducted on the effect of SA on MEK1/2 signaling in neuroblastoma cells. Thus, the aim of this study is to reveal whether SA has an effect on MEK1/2 signaling in neuroblastoma cancer which is a frequent pediatric cancer with poor prognosis. Materials and Methods: The purpose of this study was to investigate whether a SA analog acibenzolar-S-methyl had an effect on the MEK1/2 signaling pathway and on cell viability in SH-SY5Y neuroblastoma cells by MTS (3-(4,5-dimethylthiazol2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) cell viability analysis and MEK1/2 and active caspase-3 detection by western blotting technique. Results: MTS cell viability test indicated that 10 mM acibenzolar-S-methyl reduces cell viability by 50%. Western blotting results of 10 mM acibenzolar-S-methyl–treated cells showed that MEK1/2 signaling was significantly inhibited in SH-SY5H cells. Besides, an increase in active-caspase-3 levels provided insight into acibenzolar-S-methyl’s apoptotic effect which needs further morphological apoptotic data. Conclusion: Our research is the first to show that SA analog acibenzolar-S-methyl negatively affects MEK1/2 signaling causing the death of SH-SY5Y neuroblastoma cells. Our results can give insight not only into understanding the mechanisms of carcinogenesis but also into developing effective treatment methods.

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DIŞA AKTAR



APA

Yıldız, A. (2019). Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells. European Journal of Biology, 78(2), 83-88. https://doi.org/10.26650/EurJBiol.2019.0020


AMA

Yıldız A. Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells. European Journal of Biology. 2019;78(2):83-88. https://doi.org/10.26650/EurJBiol.2019.0020


ABNT

Yıldız, A. Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells. European Journal of Biology, [Publisher Location], v. 78, n. 2, p. 83-88, 2019.


Chicago: Author-Date Style

Yıldız, Ayşegül,. 2019. “Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells.” European Journal of Biology 78, no. 2: 83-88. https://doi.org/10.26650/EurJBiol.2019.0020


Chicago: Humanities Style

Yıldız, Ayşegül,. Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells.” European Journal of Biology 78, no. 2 (Nov. 2024): 83-88. https://doi.org/10.26650/EurJBiol.2019.0020


Harvard: Australian Style

Yıldız, A 2019, 'Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells', European Journal of Biology, vol. 78, no. 2, pp. 83-88, viewed 22 Nov. 2024, https://doi.org/10.26650/EurJBiol.2019.0020


Harvard: Author-Date Style

Yıldız, A. (2019) ‘Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells’, European Journal of Biology, 78(2), pp. 83-88. https://doi.org/10.26650/EurJBiol.2019.0020 (22 Nov. 2024).


MLA

Yıldız, Ayşegül,. Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells.” European Journal of Biology, vol. 78, no. 2, 2019, pp. 83-88. [Database Container], https://doi.org/10.26650/EurJBiol.2019.0020


Vancouver

Yıldız A. Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells. European Journal of Biology [Internet]. 22 Nov. 2024 [cited 22 Nov. 2024];78(2):83-88. Available from: https://doi.org/10.26650/EurJBiol.2019.0020 doi: 10.26650/EurJBiol.2019.0020


ISNAD

Yıldız, Ayşegül. Acibenzolar-S-Methyl Inhibits MEK1/2 Signaling in SH-SY5Y Neuroblastoma Cells”. European Journal of Biology 78/2 (Nov. 2024): 83-88. https://doi.org/10.26650/EurJBiol.2019.0020



ZAMAN ÇİZELGESİ


Gönderim18.07.2019
Kabul16.10.2019
Çevrimiçi Yayınlanma06.12.2019

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