Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions
Ismahan Kermani, Zohra FortasObjective: This study focused on two partners: a fungal specie, the desert truffle Tirmania pinoyi (Maire) Malençon and the perennial Cistaceae Helianthemum lippii locally known as “el gassis et terfes” which is a very important and pandemic plant species in the southern West of Algerian sahara. This study aimed to investigate the germination characteristics of ascospores extracted from T. pinoyi ascocarps. The T. pinoyi mycelial culture obtained was used for the first time as an inoculum for the mycorrhization of H. lippii (L.) Pers. under axenic conditions.
Materials and Methods: T. pinoyi (Maire) Malençon mycelium was isolated from the germination of ascospores extracted from ascocarps under sterile conditions and grown on 1% malt extract agar medium. The mycorrhizal synthesis between T. pinoyi mycelial isolate and H. lippii (L.) Pers. (natural host plant of this fungus) was placed in tubes on perlite and vermiculite impregnated with a nutrient solution for 3.5 months in axenic conditions.
Results: inoculated mycelial cultures form endomycorrhizae with H. lippii. The colonisation percentage (F%) was higher in inoculated plants grown on perlite rather than vermiculite in tubes. It was 22-41% respectively for plants on vermiculite and perlite after 2 months of culture and 46-82% for those on vermiculite and perlite after 3.5 months of cultivation.
Conclusion: In vitro symbiotic interaction between T. pinoyi mycelium and H. lippii markedly improves plant growth and positively influences the vitality of the plant.
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APA
Kermani, I., & Fortas, Z. (2019). Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions. European Journal of Biology, 0(0), -. https://doi.org/10.26650/EurJBiol.2025.1539612
AMA
Kermani I, Fortas Z. Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions. European Journal of Biology. 2019;0(0):-. https://doi.org/10.26650/EurJBiol.2025.1539612
ABNT
Kermani, I.; Fortas, Z. Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions. European Journal of Biology, [Publisher Location], v. 0, n. 0, p. -, 2019.
Chicago: Author-Date Style
Kermani, Ismahan, and Zohra Fortas. 2019. “Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions.” European Journal of Biology 0, no. 0: -. https://doi.org/10.26650/EurJBiol.2025.1539612
Chicago: Humanities Style
Kermani, Ismahan, and Zohra Fortas. “Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions.” European Journal of Biology 0, no. 0 (Mar. 2025): -. https://doi.org/10.26650/EurJBiol.2025.1539612
Harvard: Australian Style
Kermani, I & Fortas, Z 2019, 'Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions', European Journal of Biology, vol. 0, no. 0, pp. -, viewed 10 Mar. 2025, https://doi.org/10.26650/EurJBiol.2025.1539612
Harvard: Author-Date Style
Kermani, I. and Fortas, Z. (2019) ‘Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions’, European Journal of Biology, 0(0), pp. -. https://doi.org/10.26650/EurJBiol.2025.1539612 (10 Mar. 2025).
MLA
Kermani, Ismahan, and Zohra Fortas. “Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions.” European Journal of Biology, vol. 0, no. 0, 2019, pp. -. [Database Container], https://doi.org/10.26650/EurJBiol.2025.1539612
Vancouver
Kermani I, Fortas Z. Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions. European Journal of Biology [Internet]. 10 Mar. 2025 [cited 10 Mar. 2025];0(0):-. Available from: https://doi.org/10.26650/EurJBiol.2025.1539612 doi: 10.26650/EurJBiol.2025.1539612
ISNAD
Kermani, Ismahan - Fortas, Zohra. “Ascospores Germination of Tirmania pinoyi (Maire) Malençon and In Vitro Mycorrhization of Helianthemum lippii (L.) Pers. with Tirmania pinoyi Mycelial Isolate in Axenic Conditions”. European Journal of Biology 0/0 (Mar. 2025): -. https://doi.org/10.26650/EurJBiol.2025.1539612