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DOI :10.26650/EurJBiol.2025.1634721   IUP :10.26650/EurJBiol.2025.1634721    Tam Metin (PDF)

Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1

Nguyen Duy KhanhTruong Quoc Tat

Objective: Phosphatase is primarily derived from soil microorganisms, and the composition of soil microbial communities greatly influences the availability of this enzyme source. Therefore, it is crucial to research soil bacteria capable of producing phosphatase. This study evaluated the phosphatase production ability of two bacterial strains, Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1, isolated from agricultural soil under different conditions. This study was conducted in vitro to assess the effects of shaking, pH level, salt conditions, and carbon and nitrogen nutrient sources on phosphatase production ability.

Materials and Methods: Experiments were conducted under varying environmental conditions, including different shaking speeds (0, 60, 90, 120, and 150 rpm), medium pH levels (3, 5, 7, and 9), and NaCl concentrations (0, 1, 2, 3, 4, and 5%). The carbon sources used in the experiment were lactose, sucrose, maltose, and glucose, each at a concentration of 1%. The inorganic nitrogen sources were KNO₃, (NH₄)₂SO₄, (NH₄)₆Mo₇O₂₄ , and CO(NH₂)₂ at all concentrations of 10%. Each experimental condition was tested in four replications.

Results: B. vietnamiensis 1.7 and P. aryabhattai 6.1 exhibited the highest phosphatase production when cultured at a shaking speed of 150 rpm, pH 7, and a NaCl concentration of 1%, with glucose and urea as the carbon and nitrogen sources, respectively.

Conclusion: These findings indicate that optimising the culture conditions of these two bacterial strains significantly enhances phosphatase production.


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DIŞA AKTAR



APA

Khanh, N.D., & Tat, T.Q. (2019). Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1. European Journal of Biology, 0(0), -. https://doi.org/10.26650/EurJBiol.2025.1634721


AMA

Khanh N D, Tat T Q. Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1. European Journal of Biology. 2019;0(0):-. https://doi.org/10.26650/EurJBiol.2025.1634721


ABNT

Khanh, N.D.; Tat, T.Q. Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1. European Journal of Biology, [Publisher Location], v. 0, n. 0, p. -, 2019.


Chicago: Author-Date Style

Khanh, Nguyen Duy, and Truong Quoc Tat. 2019. “Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1.” European Journal of Biology 0, no. 0: -. https://doi.org/10.26650/EurJBiol.2025.1634721


Chicago: Humanities Style

Khanh, Nguyen Duy, and Truong Quoc Tat. Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1.” European Journal of Biology 0, no. 0 (Sep. 2025): -. https://doi.org/10.26650/EurJBiol.2025.1634721


Harvard: Australian Style

Khanh, ND & Tat, TQ 2019, 'Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1', European Journal of Biology, vol. 0, no. 0, pp. -, viewed 20 Sep. 2025, https://doi.org/10.26650/EurJBiol.2025.1634721


Harvard: Author-Date Style

Khanh, N.D. and Tat, T.Q. (2019) ‘Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1’, European Journal of Biology, 0(0), pp. -. https://doi.org/10.26650/EurJBiol.2025.1634721 (20 Sep. 2025).


MLA

Khanh, Nguyen Duy, and Truong Quoc Tat. Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1.” European Journal of Biology, vol. 0, no. 0, 2019, pp. -. [Database Container], https://doi.org/10.26650/EurJBiol.2025.1634721


Vancouver

Khanh ND, Tat TQ. Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1. European Journal of Biology [Internet]. 20 Sep. 2025 [cited 20 Sep. 2025];0(0):-. Available from: https://doi.org/10.26650/EurJBiol.2025.1634721 doi: 10.26650/EurJBiol.2025.1634721


ISNAD

Khanh, NguyenDuy - Tat, TruongQuoc. Evaluation of the Phosphatase Activities of the Burkholderia vietnamiensis 1.7 and Priestia aryabhattai 6.1”. European Journal of Biology 0/0 (Sep. 2025): -. https://doi.org/10.26650/EurJBiol.2025.1634721



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